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The map label for this gene is ligA

Identifier: 157961514

GI number: 157961514

Start: 2052358

End: 2054364

Strand: Direct

Name: ligA

Synonym: Spea_1689

Alternate gene names: 157961514

Gene position: 2052358-2054364 (Clockwise)

Preceding gene: 157961513

Following gene: 157961515

Centisome position: 39.66

GC content: 49.48

Gene sequence:

>2007_bases
ATGCCAGCTATTCAAAACGAAATTGCTCAGCTAACTGCTGAACTCAATCAACATAACTATCGCTATTATGTCGATGACAG
TCCTTCTATTCCCGATGCCGAGTACGACCGTTTATTGAATAGGCTTAAAGCCTTAGAGACTGAGCATCCAGAGTTTTGTC
GACCTGACTCGCCAACACAAAGAGTTGGAGGCGAGGCATTAGCTAAATTCGAGCAAATTAAACACTTAAAGCCAATGTTG
AGCTTAGATAACGTGTTTGATGAGCAAGAGTTCACTGCATTCAATCAACGGATCGTCGATAAAACAGGCAAAGACTTAAG
CTACTGCTGTGAGCCTAAGCTTGATGGACTCGCGGTGAGTATCGTCTATCGTGATGGCGTATTTGAGCGAGCCGCTACCC
GCGGCGATGGCCAAACTGGCGAAGACATTACTGAGAACGTTCGCACCATTAAATCGATCCCGTTAACACTACGTGGTGAT
AACTTCCCGCCATTGGTTGAGGTGCGCGGTGAGGTGATTATGCCTCATAAAGCATTCGATGCTCTCAACGAGCGCGCTCG
AGTAAAAGGGGATAAGCTATTTGTTAACCCTCGCAATGCTGCCGCTGGTAGTTTACGCCAGCTCGATAGTAAGATCACCG
CCAGTCGCGCCCTTGGTTTCTATGCCTACGCCTTAGGTGTGGTAGAGCCTGAAACATGGGAGCTTGCTGATAGTCATTAT
GGTCAACTTGAGCAGCTGCGATCATGGGGCGTTCCTGTGAGCCAAGAGGTTAGGGGGTGTGAGACCGTTGCCGAGGTGAT
GGCTTATTATTACGATATTCAACAGCGCCGTAGCAGCCTTGAGTTTGAAATCGATGGTGTGGTCGTAAAAGTCAATCAAA
TAGCCCATCAGCTCAGCCTAGGTTTCGTGGCCAAGGCGCCGCGCTGGGCGACAGCGTTTAAATTTCCGGCCCAAGAAGAG
ATGACCCTACTAGAAGGTGTCGATTTTCAAGTGGGGCGTACAGGCGCCGTCACACCTGTGGCTAGGCTGAAACCTGTGTT
TGTCGGTGGCGTTACCGTATCGAATGCAACACTCCATAATGCCGATGAAATTGCACGACTCGGCGTTAAGGTCGGTGACA
CCATCATCATCCGCCGCGCCGGTGACGTGATCCCACAAATTGTTGCCGTAGTGGCAGAAAAGCGTCCAGATGATGCTAAA
GATATTGTTTTCCCTGCTCATTGTCCTGTGTGCGACAGCGAAGTTGAGCGTGTAGAAGGCGAAGCCGTGACTCGTTGCAC
GGGGGGATTATTCTGTGAAGCCCAGCGTAAGGAAGCTATTAAGCACTTTGCTTCACGTAAGGCGCTGGATATCGATGGTA
TGGGCGATAAAGTTGTCGAGCAACTTATCGATAAAGAGTTAGTTGAGAGCCCTGCAGATCTTTTTAGACTCACTGCATCG
GCCATGACTATGCTTGAGCGTATGGGAATGAAGTCGGCAACTAAGTTAGTGGCCGCGATAGAAGTGGCAAAACAGACGAC
GTTTGCACGATTCCTTTATGCATTGGGTATTCGCGAAGTGGGTGAGGCAACCGCAGCTAATCTTGCTGCCTACTTTAAGA
CATTAGAAGCCCTTAAGGCGGCCAGTGCAGAAGAGTTCATCAAAGTCGATGATGTTGGCACTATTGTGGCGGCGCACCTA
GCGCACTTCTTAGCTCAGCCGCATAACCTTGAGGTTATCGACAAGCTAGTTGAGGCCGGGATCCATTGGCCTGCTGTTGA
AGAGGTTGCTGAAGAAGACCTCTCGCTTAAAGGGCAGACTTGGGTATTAACAGGCACCTTGACCCAGCTAAACCGTAATG
ATGCTAAGGCTAAATTGCAGGCATTAGGGGCAAAAGTGGCAGGGAGTGTGTCGAAAAATACCGACTGTCTAGTCGCTGGC
GCTGCAGCGGGTTCTAAGTTGACTAAGGCACAAGAGCTTGGCGTCAAGGTTATCGATGAAGAAGCCTTGATTGCAATTCT
CTCATAA

Upstream 100 bases:

>100_bases
GCGGATCAGGGCTCAGGCCTGATACGAAAGATAGGTTTTTGAAACGTAAACAGATTTGAAAGGCCGCAGCAGCGGCCTTT
GTTATTATTCGGATAATATA

Downstream 100 bases:

>100_bases
CTGCTCGACTAAATTAAAAAAGCTCCTTAGGGAGCTTTTTTAGTGAATGCTTGAAAATCTTTGATTTGTCGCCATATCTA
GTTTACTCCCGATCGATATT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MPAIQNEIAQLTAELNQHNYRYYVDDSPSIPDAEYDRLLNRLKALETEHPEFCRPDSPTQRVGGEALAKFEQIKHLKPML
SLDNVFDEQEFTAFNQRIVDKTGKDLSYCCEPKLDGLAVSIVYRDGVFERAATRGDGQTGEDITENVRTIKSIPLTLRGD
NFPPLVEVRGEVIMPHKAFDALNERARVKGDKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPETWELADSHY
GQLEQLRSWGVPVSQEVRGCETVAEVMAYYYDIQQRRSSLEFEIDGVVVKVNQIAHQLSLGFVAKAPRWATAFKFPAQEE
MTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAK
DIVFPAHCPVCDSEVERVEGEAVTRCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVESPADLFRLTAS
AMTMLERMGMKSATKLVAAIEVAKQTTFARFLYALGIREVGEATAANLAAYFKTLEALKAASAEEFIKVDDVGTIVAAHL
AHFLAQPHNLEVIDKLVEAGIHWPAVEEVAEEDLSLKGQTWVLTGTLTQLNRNDAKAKLQALGAKVAGSVSKNTDCLVAG
AAAGSKLTKAQELGVKVIDEEALIAILS

Sequences:

>Translated_668_residues
MPAIQNEIAQLTAELNQHNYRYYVDDSPSIPDAEYDRLLNRLKALETEHPEFCRPDSPTQRVGGEALAKFEQIKHLKPML
SLDNVFDEQEFTAFNQRIVDKTGKDLSYCCEPKLDGLAVSIVYRDGVFERAATRGDGQTGEDITENVRTIKSIPLTLRGD
NFPPLVEVRGEVIMPHKAFDALNERARVKGDKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPETWELADSHY
GQLEQLRSWGVPVSQEVRGCETVAEVMAYYYDIQQRRSSLEFEIDGVVVKVNQIAHQLSLGFVAKAPRWATAFKFPAQEE
MTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAK
DIVFPAHCPVCDSEVERVEGEAVTRCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVESPADLFRLTAS
AMTMLERMGMKSATKLVAAIEVAKQTTFARFLYALGIREVGEATAANLAAYFKTLEALKAASAEEFIKVDDVGTIVAAHL
AHFLAQPHNLEVIDKLVEAGIHWPAVEEVAEEDLSLKGQTWVLTGTLTQLNRNDAKAKLQALGAKVAGSVSKNTDCLVAG
AAAGSKLTKAQELGVKVIDEEALIAILS
>Mature_667_residues
PAIQNEIAQLTAELNQHNYRYYVDDSPSIPDAEYDRLLNRLKALETEHPEFCRPDSPTQRVGGEALAKFEQIKHLKPMLS
LDNVFDEQEFTAFNQRIVDKTGKDLSYCCEPKLDGLAVSIVYRDGVFERAATRGDGQTGEDITENVRTIKSIPLTLRGDN
FPPLVEVRGEVIMPHKAFDALNERARVKGDKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPETWELADSHYG
QLEQLRSWGVPVSQEVRGCETVAEVMAYYYDIQQRRSSLEFEIDGVVVKVNQIAHQLSLGFVAKAPRWATAFKFPAQEEM
TLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAKD
IVFPAHCPVCDSEVERVEGEAVTRCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVESPADLFRLTASA
MTMLERMGMKSATKLVAAIEVAKQTTFARFLYALGIREVGEATAANLAAYFKTLEALKAASAEEFIKVDDVGTIVAAHLA
HFLAQPHNLEVIDKLVEAGIHWPAVEEVAEEDLSLKGQTWVLTGTLTQLNRNDAKAKLQALGAKVAGSVSKNTDCLVAGA
AAGSKLTKAQELGVKVIDEEALIAILS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=62.1460506706408, Blast_Score=868, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=529, Percent_Identity=23.2514177693762, Blast_Score=116, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SHEPA (A8H376)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501548.1
- ProteinModelPortal:   A8H376
- STRING:   A8H376
- GeneID:   5662085
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1689
- NMPDR:   fig|398579.3.peg.1456
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK906920
- BioCyc:   SPEA398579:SPEA_1689-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73113; Mature: 72982

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 113-113 BINDING 111-111 BINDING 134-134 BINDING 171-171 BINDING 290-290 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIQNEIAQLTAELNQHNYRYYVDDSPSIPDAEYDRLLNRLKALETEHPEFCRPDSPTQ
CCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RVGGEALAKFEQIKHLKPMLSLDNVFDEQEFTAFNQRIVDKTGKDLSYCCEPKLDGLAVS
HHCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEE
IVYRDGVFERAATRGDGQTGEDITENVRTIKSIPLTLRGDNFPPLVEVRGEVIMPHKAFD
EEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHHCCCCEECCHHHHH
ALNERARVKGDKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPETWELADSHY
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GQLEQLRSWGVPVSQEVRGCETVAEVMAYYYDIQQRRSSLEFEIDGVVVKVNQIAHQLSL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEHHHHHHHHHH
GFVAKAPRWATAFKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHN
HHHCCCCCCHHEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECEEECCCCCCC
ADEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAKDIVFPAHCPVCDSEVERVEG
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHCC
EAVTRCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVESPADLFRLTAS
CHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
AMTMLERMGMKSATKLVAAIEVAKQTTFARFLYALGIREVGEATAANLAAYFKTLEALKA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASAEEFIKVDDVGTIVAAHLAHFLAQPHNLEVIDKLVEAGIHWPAVEEVAEEDLSLKGQT
CCHHHHEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCE
WVLTGTLTQLNRNDAKAKLQALGAKVAGSVSKNTDCLVAGAAAGSKLTKAQELGVKVIDE
EEEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHCCCEEECC
EALIAILS
CCEEEECC
>Mature Secondary Structure 
PAIQNEIAQLTAELNQHNYRYYVDDSPSIPDAEYDRLLNRLKALETEHPEFCRPDSPTQ
CCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RVGGEALAKFEQIKHLKPMLSLDNVFDEQEFTAFNQRIVDKTGKDLSYCCEPKLDGLAVS
HHCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEE
IVYRDGVFERAATRGDGQTGEDITENVRTIKSIPLTLRGDNFPPLVEVRGEVIMPHKAFD
EEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHHCCCCEECCHHHHH
ALNERARVKGDKLFVNPRNAAAGSLRQLDSKITASRALGFYAYALGVVEPETWELADSHY
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GQLEQLRSWGVPVSQEVRGCETVAEVMAYYYDIQQRRSSLEFEIDGVVVKVNQIAHQLSL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEHHHHHHHHHH
GFVAKAPRWATAFKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHN
HHHCCCCCCHHEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECEEECCCCCCC
ADEIARLGVKVGDTIIIRRAGDVIPQIVAVVAEKRPDDAKDIVFPAHCPVCDSEVERVEG
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHCC
EAVTRCTGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKELVESPADLFRLTAS
CHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
AMTMLERMGMKSATKLVAAIEVAKQTTFARFLYALGIREVGEATAANLAAYFKTLEALKA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASAEEFIKVDDVGTIVAAHLAHFLAQPHNLEVIDKLVEAGIHWPAVEEVAEEDLSLKGQT
CCHHHHEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCE
WVLTGTLTQLNRNDAKAKLQALGAKVAGSVSKNTDCLVAGAAAGSKLTKAQELGVKVIDE
EEEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHCCCEEECC
EALIAILS
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA