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The map label for this gene is purF [H]

Identifier: 157961447

GI number: 157961447

Start: 1962075

End: 1963589

Strand: Direct

Name: purF [H]

Synonym: Spea_1621

Alternate gene names: 157961447

Gene position: 1962075-1963589 (Clockwise)

Preceding gene: 157961446

Following gene: 157961451

Centisome position: 37.92

GC content: 45.08

Gene sequence:

>1515_bases
ATGTGTGGTATTGTCGGAATAGTTGGCCAGTCATCGGTTAATCAAACCATTTATGATGCACTGACTGTGCTCCAGCATCG
TGGACAGGATGCTGCTGGCATTGTGACCGTCGATGGCAGCGCGTTTAGATTGCGTAAAGCCAATGGTTTGGTGAAAGACG
TTTTTGAACCAAAGCATATGCAGCGTCTACAAGGCAATGCAGGCATAGGTCACGTTCGTTACCCTACGGCAGGAAGCTCT
AGCGCTTCTGAAGCTCAGCCGTTTTATGTTAACTCTCCTTTTGGTATTTCATTGGCGCACAATGGTAACTTGACCAATAC
GGTTGAGCTGCATGAGCGTTTATTGAAGCAGCGCCGTCATGTTAATACCACATCAGACTCTGAAGTGTTACTTAACTTAC
TCGCCGATGAACTTCAGCAATCTACAACAGATGATCTTAGCGCTGAAGATGTGTTTAATGCTGTTGCCAAGGTGCATGAT
ATTACTCGTGGTGCCTATGCGGTAGCTGCCATGATTATCGGCCAAGGGCTAGTTGCTTTTAGAGATCCATTTGGTATTCG
TCCGTTAGTGCTAGGTAAGCATGAAACGGCGTCAGGCACCGAATACATGATCGCATCTGAAAGCGTTGCGTTAGATGCGG
TGGGTTTTGAGTTTATGCGTGACGTAGCGCCTGGTGAGGCGGTTTATATTACCCTTGATGGTGAGCTATATACTCGCCAA
TGCGCACACTCGCCAAGCTACTCTCCTTGTATCTTTGAATTCGTATATTTTGCTCGTCCTGACTCAACCATTGATAACAT
TTCTGTTTATAGCAGCCGTGTTAATATGGGCACCATGTTGGGCGAAAAAATTAAGAGAGAGTGGGAAGATCATGATATCG
ATGTAGTTATCCCGATCCCTGAAACCTCTTGCGATATCGCACTCGAAATTGCACGTCATATGGATCTGCCATACCGTCAG
GGCTTCGTTAAAAATCGCTATATCGGCCGTACTTTTATCATGCCAGGTCAGCAAGAGCGTAAGAAGTCTGTACGCCGTAA
GCTCAACGCGATTGGTGCAGAGTTTAAAGGCAAGAACGTATTACTTGTTGATGACTCTATTGTGCGTGGTACCACGTCTG
AGCAGATCATCGAGATGGCACGTGATGCTGGCGCTAATAAAGTGTATTTCGCGTCAGCAGCACCAGAAATTCGTTTCCCG
AATGTGTACGGTATCGATATGCCAACCACAAATGAACTCATTGCCCATGGACGCGATGTTGAAGAGATCAATAACTTAAT
CGGCGCCGACGGCATGATTTTCCAATCGCTACCGGATTTAATTGAAGCGGTACGTAAAGAAAATCCAAGTATTAAGCGTT
TTGAAACCTCTGTATTTGACGGTAAGTACGTCACCAATGACGTAAATCAAGAGTACTTAGATTACATTACACAATTGCGT
AACGATGATGCTAAGGCAAATCGTAGCAAAGATATTGGCACTAACTTAGAGATGCATAACGTCTGTCATCCTTAA

Upstream 100 bases:

>100_bases
AAATGCACTAAAGCATTACTTGGTTTTAGGTAGAGACGTCAGTCTCACCTAAAGCAAAAATATTAAATAATAGAACATCT
TACAATGAGGAAGCTTACCC

Downstream 100 bases:

>100_bases
GCAAGTGTCTAGTCCTAAAATAGGTTGACGGTTTTTATTAAGCCAGTTGGAACTCCCGACTGGCTTTTTTATGTACTTCA
TTAGGGGTTTTCATCCCTAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELHERLLKQRRHVNTTSDSEVLLNLLADELQQSTTDDLSAEDVFNAVAKVHD
ITRGAYAVAAMIIGQGLVAFRDPFGIRPLVLGKHETASGTEYMIASESVALDAVGFEFMRDVAPGEAVYITLDGELYTRQ
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIPETSCDIALEIARHMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAIGAEFKGKNVLLVDDSIVRGTTSEQIIEMARDAGANKVYFASAAPEIRFP
NVYGIDMPTTNELIAHGRDVEEINNLIGADGMIFQSLPDLIEAVRKENPSIKRFETSVFDGKYVTNDVNQEYLDYITQLR
NDDAKANRSKDIGTNLEMHNVCHP

Sequences:

>Translated_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELHERLLKQRRHVNTTSDSEVLLNLLADELQQSTTDDLSAEDVFNAVAKVHD
ITRGAYAVAAMIIGQGLVAFRDPFGIRPLVLGKHETASGTEYMIASESVALDAVGFEFMRDVAPGEAVYITLDGELYTRQ
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIPETSCDIALEIARHMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAIGAEFKGKNVLLVDDSIVRGTTSEQIIEMARDAGANKVYFASAAPEIRFP
NVYGIDMPTTNELIAHGRDVEEINNLIGADGMIFQSLPDLIEAVRKENPSIKRFETSVFDGKYVTNDVNQEYLDYITQLR
NDDAKANRSKDIGTNLEMHNVCHP
>Mature_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELHERLLKQRRHVNTTSDSEVLLNLLADELQQSTTDDLSAEDVFNAVAKVHD
ITRGAYAVAAMIIGQGLVAFRDPFGIRPLVLGKHETASGTEYMIASESVALDAVGFEFMRDVAPGEAVYITLDGELYTRQ
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIPETSCDIALEIARHMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAIGAEFKGKNVLLVDDSIVRGTTSEQIIEMARDAGANKVYFASAAPEIRFP
NVYGIDMPTTNELIAHGRDVEEINNLIGADGMIFQSLPDLIEAVRKENPSIKRFETSVFDGKYVTNDVNQEYLDYITQLR
NDDAKANRSKDIGTNLEMHNVCHP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=36.2105263157895, Blast_Score=279, Evalue=5e-75,
Organism=Escherichia coli, GI1788651, Length=503, Percent_Identity=73.558648111332, Blast_Score=760, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=32.7354260089686, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=32.7800829875519, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6323958, Length=508, Percent_Identity=50.9842519685039, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24659598, Length=493, Percent_Identity=34.2799188640974, Blast_Score=275, Evalue=7e-74,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=34.9680170575693, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI28573187, Length=472, Percent_Identity=34.3220338983051, Blast_Score=258, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55796; Mature: 55796

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHCCHHHHHCCHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELHERLLKQRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQQSTTDDLSAEDVFNAVAKVHDITRGAYAVAAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEE
RDPFGIRPLVLGKHETASGTEYMIASESVALDAVGFEFMRDVAPGEAVYITLDGELYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCEEHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARHMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAIGAEFKGKNV
CCCHHHHHHHHHHCCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMARDAGANKVYFASAAPEIRFPNVYGIDMPTTNELIAHGRDV
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHCCCCH
EEINNLIGADGMIFQSLPDLIEAVRKENPSIKRFETSVFDGKYVTNDVNQEYLDYITQLR
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHEEHHHCCCCEECCCCCHHHHHHHHHHC
NDDAKANRSKDIGTNLEMHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHCCHHHHHCCHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELHERLLKQRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQQSTTDDLSAEDVFNAVAKVHDITRGAYAVAAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEE
RDPFGIRPLVLGKHETASGTEYMIASESVALDAVGFEFMRDVAPGEAVYITLDGELYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCEEHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARHMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAIGAEFKGKNV
CCCHHHHHHHHHHCCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMARDAGANKVYFASAAPEIRFPNVYGIDMPTTNELIAHGRDV
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHCCCCH
EEINNLIGADGMIFQSLPDLIEAVRKENPSIKRFETSVFDGKYVTNDVNQEYLDYITQLR
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHEEHHHCCCCEECCCCCHHHHHHHHHHC
NDDAKANRSKDIGTNLEMHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]