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The map label for this gene is iscS [H]

Identifier: 157961314

GI number: 157961314

Start: 1809887

End: 1811101

Strand: Direct

Name: iscS [H]

Synonym: Spea_1488

Alternate gene names: 157961314

Gene position: 1809887-1811101 (Clockwise)

Preceding gene: 157961313

Following gene: 157961315

Centisome position: 34.98

GC content: 45.6

Gene sequence:

>1215_bases
ATGAAGCTACCTATCTATCTAGATTATGCTGCGACGACGCCGGTTGACCCTCGTGTCGCAGAGAAAATGATGCAATGCCT
GACTATGGACGGCATTTTCGGCAATCCAGCGTCTCGTTCTCACCGTTACGGTTGGCAAGCTGAAGAAGCTGTCGATATCG
CTCGTAACCAAGTTGCAGAGCTGATTAATGCCGACCCGCGTGAAATCGTGTTTACTTCAGGTGCGACAGAGTCTGACAAC
CTTGCAATTAAAGGTGTTGCTCACTTCTACCATAAGAAGGGTAAGCACATCATCACTAGCAAGACTGAACATAAGGCCGT
ATTAGATACTTGTCGCCAACTTGAGCGTGAAGGTTATGAAGTGACTTATCTTCAGCCTGAGCCAAGTGGTTTGATCCCTG
TATCTATGATTGAAGCCGCTATGCGTGAAGACACAATTTTAGTGAGCATCATGCAAGTGAACAACGAGATTGGTGTTATT
CAAGATATCGATGCTATCGGCGAGCTTTGTCGTTCACGTAAAATCATATTCCACGTCGATGCGGCGCAAAGCGCGGGTAA
ATTACCTATCGATGTGCAAACGACAAAAGTTGATCTAATGTCTATCTCTGGGCACAAGATGTATGGCCCTAAAGGTATCG
GTGCGCTTTATGTCAGCCGTAAGCCGCGTATTCGCCTAGAAGCAGCAATGCATGGTGGCGGACATGAACGCGGTATGCGT
AGTGGTACTCTTGCGACTCACCAAATTGTGGGTATGGGCGAAGCTGCTGCTATTGCTAAAGCTGATATGGAAGTTGATAA
CGAACGTATTCGTCGTTTACGCGATAAGTTATGGAACGGCATCAATCACATCGAAGAAACCTATATTAATGGTGACGTGG
AAAAAAGAGCCTGCGGTAGCTTAAACGTGAGCTTTAACTTCGTTGAAGGTGAATCATTGATGATGGCACTGAAAGACCTA
GCGGTTTCATCGGGCTCAGCATGTACTTCAGCAAGTCTTGAACCAAGTTATGTTCTACGTGCACTTGGTCTAAATGACGA
AATGGCTCATAGCTCAATTCGATTCTCAATCGGTCGCTTTACGACAGATGAAGAGATCGACCATGCAATAGAAACGATTA
AAGAATCTATTGGTAATTTAAGGGAGATGTCTCCGCTTTGGGAAATGTTCAAAGACGGTATCGATCTGGACTCTGTCCAG
TGGGCACATCACTAA

Upstream 100 bases:

>100_bases
AAAGGCTAAGTATTTAATAGCTTTTGTTATGAATATAAAAATTATTTTTTGTACGTTGTTAAGTGGCCTCCATTAGAGGC
TACAAAGTACGGAGTGTGTG

Downstream 100 bases:

>100_bases
CCTTAACTTAATTCACGAATAGATAAATTGGAGTAGTATCATGGCTTATAGCGAAAAAGTAATCGATCATTATGAAAATC
CGCGTAACGTCGGTTCGTTT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAELINADPREIVFTSGATESDN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYEVTYLQPEPSGLIPVSMIEAAMREDTILVSIMQVNNEIGVI
QDIDAIGELCRSRKIIFHVDAAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
SGTLATHQIVGMGEAAAIAKADMEVDNERIRRLRDKLWNGINHIEETYINGDVEKRACGSLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQ
WAHH

Sequences:

>Translated_404_residues
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAELINADPREIVFTSGATESDN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYEVTYLQPEPSGLIPVSMIEAAMREDTILVSIMQVNNEIGVI
QDIDAIGELCRSRKIIFHVDAAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
SGTLATHQIVGMGEAAAIAKADMEVDNERIRRLRDKLWNGINHIEETYINGDVEKRACGSLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQ
WAHH
>Mature_404_residues
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAELINADPREIVFTSGATESDN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYEVTYLQPEPSGLIPVSMIEAAMREDTILVSIMQVNNEIGVI
QDIDAIGELCRSRKIIFHVDAAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
SGTLATHQIVGMGEAAAIAKADMEVDNERIRRLRDKLWNGINHIEETYINGDVEKRACGSLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQ
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=58.7064676616915, Blast_Score=509, Evalue=1e-144,
Organism=Homo sapiens, GI156713448, Length=419, Percent_Identity=31.5035799522673, Blast_Score=191, Evalue=8e-49,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=78.4653465346535, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=31.6279069767442, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787970, Length=224, Percent_Identity=32.1428571428571, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=58.8089330024814, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=33.3333333333333, Blast_Score=154, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.6059850374065, Blast_Score=492, Evalue=1e-140,
Organism=Drosophila melanogaster, GI20129463, Length=405, Percent_Identity=57.2839506172839, Blast_Score=489, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44725; Mature: 44725

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADPREIVFTSGATESDNLAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYE
HHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLQPEPSGLIPVSMIEAAMREDTILVSIMQVNNEIGVIQDIDAIGELCRSRKIIFHVD
EEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEECHHHHHHHHHCCEEEEEEE
AAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCEEEEECCCCCEEEEEEECCCCHHCCCC
SGTLATHQIVGMGEAAAIAKADMEVDNERIRRLRDKLWNGINHIEETYINGDVEKRACGS
CCCCHHHHEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
LNVSFNFVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
CEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEEEECCC
TTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADPREIVFTSGATESDNLAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYE
HHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLQPEPSGLIPVSMIEAAMREDTILVSIMQVNNEIGVIQDIDAIGELCRSRKIIFHVD
EEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEECHHHHHHHHHCCEEEEEEE
AAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCEEEEECCCCCEEEEEEECCCCHHCCCC
SGTLATHQIVGMGEAAAIAKADMEVDNERIRRLRDKLWNGINHIEETYINGDVEKRACGS
CCCCHHHHEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
LNVSFNFVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
CEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHEEEECCC
TTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA