Click here to switch to the map view.

The map label for this gene is glpE [C]

Identifier: 157961301

GI number: 157961301

Start: 1798059

End: 1798418

Strand: Direct

Name: glpE [C]

Synonym: Spea_1475

Alternate gene names: 157961301

Gene position: 1798059-1798418 (Clockwise)

Preceding gene: 157961300

Following gene: 157961302

Centisome position: 34.75

GC content: 43.61

Gene sequence:

>360_bases
ATGAAACACAATCCCGCATTTTTAGCGTTAGTTGAAAGTATTTTGCCTTTAGTGACAGAGGTGAATGTTGAGCAATATCA
AACGATGAATGACTGGACGTTACTTGACGTTCGTGAAGATTCTGAGTGGTTGAAAGGGCGTTTACCTAACGCGCTACATC
TTGGTCGTGGCATTATTGAAAGAGACATCGAGACACTTTTCCCTGAAAAAGATACCCCGCTAGTTTTGTACTGTGGTGGC
GGATATCGTTCAGCGTTAGCGGCGAACAACCTTCAGGTTATGGGTTATACCAAAGTGGTATCCTTAGCCGGTGGTTATAA
AGCGTGGCTTGAGCGACAATTGCCGATAGAAAATGACTAA

Upstream 100 bases:

>100_bases
ACTGTCCACAATGGAAGTGCGTTAATTGCCATGAAATCAATGAATCAAGTTTCGAGCTCTGCTGGAATTGCAGCGCCGAA
CGCAGTGAGAAGTACAGTTA

Downstream 100 bases:

>100_bases
GCTTGCCTGTATTCGTCGATAACTAAGGCTACGGGTCGCTAGTCGGTTAATATCGTTATTATTCGAAAAGGTGTAACTGC
TCAATGCAATATTTTCCGCT

Product: rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: Rhodanese Domain Protein; Rhodanese-Like Protein; Molybdopterin Biosynthesis Protein; Molybdopterin Biosynthesis Protein MoeB; Beta-Lactamase Domain Protein; Rhodanese-Like Domain-Containing Protein; Metallo-Beta-Lactamase Superfamily Protein; Molybdopterin Biosynthesis MoeB Protein; Rhodanese-Like Family Protein; Thiosulfate Sulfurtransferase GlpE; Rhodanese-Like Domain Protein; Rhodanese/Sulfurtransferase-Like Protein; Metallo-Beta-Lactamase/Rhodanese-Like Domain Protein; Rhodanese-Domain Protein; Rhodanese/Moeb/Thif Domain Protein; Rhodanese-Related Sulfurtransferase

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MKHNPAFLALVESILPLVTEVNVEQYQTMNDWTLLDVREDSEWLKGRLPNALHLGRGIIERDIETLFPEKDTPLVLYCGG
GYRSALAANNLQVMGYTKVVSLAGGYKAWLERQLPIEND

Sequences:

>Translated_119_residues
MKHNPAFLALVESILPLVTEVNVEQYQTMNDWTLLDVREDSEWLKGRLPNALHLGRGIIERDIETLFPEKDTPLVLYCGG
GYRSALAANNLQVMGYTKVVSLAGGYKAWLERQLPIEND
>Mature_119_residues
MKHNPAFLALVESILPLVTEVNVEQYQTMNDWTLLDVREDSEWLKGRLPNALHLGRGIIERDIETLFPEKDTPLVLYCGG
GYRSALAANNLQVMGYTKVVSLAGGYKAWLERQLPIEND

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 13444; Mature: 13444

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHNPAFLALVESILPLVTEVNVEQYQTMNDWTLLDVREDSEWLKGRLPNALHLGRGIIE
CCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHH
RDIETLFPEKDTPLVLYCGGGYRSALAANNLQVMGYTKVVSLAGGYKAWLERQLPIEND
HHHHHHCCCCCCCEEEEECCCCCHHHHCCCEEEEEHHHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKHNPAFLALVESILPLVTEVNVEQYQTMNDWTLLDVREDSEWLKGRLPNALHLGRGIIE
CCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHH
RDIETLFPEKDTPLVLYCGGGYRSALAANNLQVMGYTKVVSLAGGYKAWLERQLPIEND
HHHHHHCCCCCCCEEEEECCCCCHHHHCCCEEEEEHHHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA