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The map label for this gene is ywbI [H]

Identifier: 157961279

GI number: 157961279

Start: 1767553

End: 1768434

Strand: Reverse

Name: ywbI [H]

Synonym: Spea_1453

Alternate gene names: 157961279

Gene position: 1768434-1767553 (Counterclockwise)

Preceding gene: 157961281

Following gene: 157961273

Centisome position: 34.18

GC content: 45.58

Gene sequence:

>882_bases
ATGGATATTAAACAGCTAAAATACTTAGATGCCTTAATTACAGCGGGTAGCTTCACTAAAGCCGCCGAGCGGCTCAATAT
GGCGCAGCCAGCGTTGAGTCAAAGCATTAAGCGCTTAGAGCAGGAGCTCGGCGTCACCCTAGTTGATAGAGGGGCGAATA
AAAGCAGTAAAACACTTTCGCTAACTGCTGAAGGCAGCGCTCTACATCAGCATGCCAAGCTGATAATCAAAAATATGGCG
CAAGCTGAAGCGCACATAAAATCGATGGCAAACCTGACGATGGGCGAAGTCAGAGTTGCAGTGCCTGGTATGCTAGGGTC
TTATTATCTACCTAGGCGATTAATGGCATTTCGCCACCAACATTCAGAACTTAAATTATCCCTGTTTGAAGGCGGTACTC
GAGACACCCTCAGAATGCTACAGAATGAAGATGTCGATATCGCCATCATTACCGCCAACGATCTGACCGATGAGTTTGAC
AGTCACCTACTACTGCAGGAGCAGATGGTCGTCGCAGTGGGTAAAGAACACCCTTTAGCCAAATTAGATACGGTGACGCT
TCAAGCGTTTTTCCAGCATGAACTGGTATTCTTTAAACCGGGTTACTTTCACCGTGATTGGATGCTTGAACAGGCGGAGA
AATTAGGCCTTAAGGCAAATATCGCCTTTGAAACCAACCTGATTAATCTCATTAAACAAGTTGTTGCACAGGAGTATGGA
ATAACCAGTGTGCTTGAAATGGTGATAGAAAAACACGACAACATTTGCGCCAAGCCCTTCAACCCACCTATCTTTTTGGA
TTTGCATATTGCTTGGAAGAAACAACGCCCTATCAGTCAAGCCGACAAGGCGTTCGTTGAGTTTCTGATGCAAAACCGTT
AG

Upstream 100 bases:

>100_bases
AATCTAGAACTCCTATAGACTGGTAAACATACACCAATAGCAATAGGTCCATCTCACTTCAGTGCGACCTGCTTATTTAG
CAAAACTCAGTAGAGCAAAG

Downstream 100 bases:

>100_bases
CTAGGTAATTACAGATCCCTGTTATGCTGCATTAAGTGCTATATATCGCCCTAGCTGATAAAAGCGCTCATTGAGTTTCT
ATGCGACACTGCTAGCTGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDIKQLKYLDALITAGSFTKAAERLNMAQPALSQSIKRLEQELGVTLVDRGANKSSKTLSLTAEGSALHQHAKLIIKNMA
QAEAHIKSMANLTMGEVRVAVPGMLGSYYLPRRLMAFRHQHSELKLSLFEGGTRDTLRMLQNEDVDIAIITANDLTDEFD
SHLLLQEQMVVAVGKEHPLAKLDTVTLQAFFQHELVFFKPGYFHRDWMLEQAEKLGLKANIAFETNLINLIKQVVAQEYG
ITSVLEMVIEKHDNICAKPFNPPIFLDLHIAWKKQRPISQADKAFVEFLMQNR

Sequences:

>Translated_293_residues
MDIKQLKYLDALITAGSFTKAAERLNMAQPALSQSIKRLEQELGVTLVDRGANKSSKTLSLTAEGSALHQHAKLIIKNMA
QAEAHIKSMANLTMGEVRVAVPGMLGSYYLPRRLMAFRHQHSELKLSLFEGGTRDTLRMLQNEDVDIAIITANDLTDEFD
SHLLLQEQMVVAVGKEHPLAKLDTVTLQAFFQHELVFFKPGYFHRDWMLEQAEKLGLKANIAFETNLINLIKQVVAQEYG
ITSVLEMVIEKHDNICAKPFNPPIFLDLHIAWKKQRPISQADKAFVEFLMQNR
>Mature_293_residues
MDIKQLKYLDALITAGSFTKAAERLNMAQPALSQSIKRLEQELGVTLVDRGANKSSKTLSLTAEGSALHQHAKLIIKNMA
QAEAHIKSMANLTMGEVRVAVPGMLGSYYLPRRLMAFRHQHSELKLSLFEGGTRDTLRMLQNEDVDIAIITANDLTDEFD
SHLLLQEQMVVAVGKEHPLAKLDTVTLQAFFQHELVFFKPGYFHRDWMLEQAEKLGLKANIAFETNLINLIKQVVAQEYG
ITSVLEMVIEKHDNICAKPFNPPIFLDLHIAWKKQRPISQADKAFVEFLMQNR

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=262, Percent_Identity=29.3893129770992, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790399, Length=266, Percent_Identity=24.812030075188, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693105, Length=293, Percent_Identity=25.2559726962457, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786401, Length=311, Percent_Identity=25.08038585209, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI2367136, Length=290, Percent_Identity=25.5172413793103, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787879, Length=204, Percent_Identity=27.4509803921569, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787601, Length=180, Percent_Identity=28.3333333333333, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788481, Length=297, Percent_Identity=24.9158249158249, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788296, Length=246, Percent_Identity=26.4227642276423, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=27.4193548387097, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=22.8373702422145, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788887, Length=190, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787530, Length=257, Percent_Identity=23.3463035019455, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=256, Percent_Identity=24.21875, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33123; Mature: 33123

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKQLKYLDALITAGSFTKAAERLNMAQPALSQSIKRLEQELGVTLVDRGANKSSKTLS
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEECCCCCCCCEEE
LTAEGSALHQHAKLIIKNMAQAEAHIKSMANLTMGEVRVAVPGMLGSYYLPRRLMAFRHQ
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCHHHHHHHHHHHHHHHHC
HSELKLSLFEGGTRDTLRMLQNEDVDIAIITANDLTDEFDSHLLLQEQMVVAVGKEHPLA
CCCEEEEEECCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCH
KLDTVTLQAFFQHELVFFKPGYFHRDWMLEQAEKLGLKANIAFETNLINLIKQVVAQEYG
HHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCC
ITSVLEMVIEKHDNICAKPFNPPIFLDLHIAWKKQRPISQADKAFVEFLMQNR
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIKQLKYLDALITAGSFTKAAERLNMAQPALSQSIKRLEQELGVTLVDRGANKSSKTLS
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEECCCCCCCCEEE
LTAEGSALHQHAKLIIKNMAQAEAHIKSMANLTMGEVRVAVPGMLGSYYLPRRLMAFRHQ
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCHHHHHHHHHHHHHHHHC
HSELKLSLFEGGTRDTLRMLQNEDVDIAIITANDLTDEFDSHLLLQEQMVVAVGKEHPLA
CCCEEEEEECCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCH
KLDTVTLQAFFQHELVFFKPGYFHRDWMLEQAEKLGLKANIAFETNLINLIKQVVAQEYG
HHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCC
ITSVLEMVIEKHDNICAKPFNPPIFLDLHIAWKKQRPISQADKAFVEFLMQNR
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]