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The map label for this gene is spsK [H]

Identifier: 157961263

GI number: 157961263

Start: 1748985

End: 1750076

Strand: Direct

Name: spsK [H]

Synonym: Spea_1437

Alternate gene names: 157961263

Gene position: 1748985-1750076 (Clockwise)

Preceding gene: 157961262

Following gene: 157961265

Centisome position: 33.8

GC content: 45.42

Gene sequence:

>1092_bases
ATGAGAATTCTTATCACAGGAGCCGCAGGTCAACTTGGACAGGCATTGCTATCGATAGCGGGGCTTACACAGGTTAATTT
GGCAGAGCGAACCGTCGCTCAGCAAATGCTGGTGGCTTTGTTACCCGAAGCGTTAGAGTGCATCGAAACAACAGATGAAG
TGATTGGTGTTTCTCATCAAGCGCTCGATATTTGCGATATAGATTCAATACGTAAAGCATTCGATACTATAGCTCCCGAT
GTTGTGATTAACTGCGCCGCCTATAACGCAGTTGATAAGGCTGAGTTTGATATCGACAAGGCAATGCTTATTAATGCCGA
AGGGCCTAAATTACTCGCCGGTGAGTGTCAGCGCCACAATATAAGACTCGTGCATATCTCAACTGACTTTGTGTTCGATG
GTGAGTTATTACGTGCATACACAGAGCAAGATAGCCCCGCGCCGCTGTCGGTTTATGGCAAGAGTAAGCTAGAAGGGGAG
CGCTGGGTCAGTGATATCTTGGGCTCAAAGGCAACGATTATTCGTACCTCTTGGCTCTACAGTTGTTATGGCCATAATTT
TGTTAAAACCATGCAGAGTTTGTTTAAGGTCAAAGAGAGCCTGTCGGTAATAAACGATCAATATGGTAGCCCTACTTGGT
GTGAAGCCTTAGCCGTAGCAATTTTTATGCTCGTAAAGCAAACTCAGGCGACAATTTTGACTCGCCCGTCAGTATGCGAG
GATAAACCCGCTAAAGGCTTAGCTGATTTATACCACTATGCTGCTAGTGCTAGCGCTTCATGGTATGAGTTTGCCTGTGA
AATTCAACGTCAGGCAGGCTCTGTCACAGAGCTCGCACCAAGCACAAAATTATCACAAAACAAAGAATTCGCACCAAACA
GTGCTGATTGTTCAATACTGCCCATTACGACTAAAGCTTGGCAAGAGTTACATGATAACCGATTAGCATTAAGGCCAAAG
CAAAGTGCTTTGAATACGCAAAAGTTGTGTCATCAGTTAGCTATCGAGCCGGGGAGTCTACTCACTGCAACTTGGCAGCA
ACAGTTAGCAGCCATGGTTAGCTATCAAAAGGTTAAAAGCCGGGAAAGCTGA

Upstream 100 bases:

>100_bases
AAGAGGCCATGGCTCGCTCGATGAATATTCGGCGCTACTCGAAAACTAAAACCAAAACTAAAACTAAAACCAAAAAGATA
GCAGAGATAATTAAAGTAAG

Downstream 100 bases:

>100_bases
GAAGGTCCTAGGAAAAGCAGGTCCTAGGTTAAAGCTAAGATGGAAAAGCGGATCGCCCTGCGGCCTTACGGTAAACGGAA
GGTTCGCATACTCACTCACG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRILITGAAGQLGQALLSIAGLTQVNLAERTVAQQMLVALLPEALECIETTDEVIGVSHQALDICDIDSIRKAFDTIAPD
VVINCAAYNAVDKAEFDIDKAMLINAEGPKLLAGECQRHNIRLVHISTDFVFDGELLRAYTEQDSPAPLSVYGKSKLEGE
RWVSDILGSKATIIRTSWLYSCYGHNFVKTMQSLFKVKESLSVINDQYGSPTWCEALAVAIFMLVKQTQATILTRPSVCE
DKPAKGLADLYHYAASASASWYEFACEIQRQAGSVTELAPSTKLSQNKEFAPNSADCSILPITTKAWQELHDNRLALRPK
QSALNTQKLCHQLAIEPGSLLTATWQQQLAAMVSYQKVKSRES

Sequences:

>Translated_363_residues
MRILITGAAGQLGQALLSIAGLTQVNLAERTVAQQMLVALLPEALECIETTDEVIGVSHQALDICDIDSIRKAFDTIAPD
VVINCAAYNAVDKAEFDIDKAMLINAEGPKLLAGECQRHNIRLVHISTDFVFDGELLRAYTEQDSPAPLSVYGKSKLEGE
RWVSDILGSKATIIRTSWLYSCYGHNFVKTMQSLFKVKESLSVINDQYGSPTWCEALAVAIFMLVKQTQATILTRPSVCE
DKPAKGLADLYHYAASASASWYEFACEIQRQAGSVTELAPSTKLSQNKEFAPNSADCSILPITTKAWQELHDNRLALRPK
QSALNTQKLCHQLAIEPGSLLTATWQQQLAAMVSYQKVKSRES
>Mature_363_residues
MRILITGAAGQLGQALLSIAGLTQVNLAERTVAQQMLVALLPEALECIETTDEVIGVSHQALDICDIDSIRKAFDTIAPD
VVINCAAYNAVDKAEFDIDKAMLINAEGPKLLAGECQRHNIRLVHISTDFVFDGELLRAYTEQDSPAPLSVYGKSKLEGE
RWVSDILGSKATIIRTSWLYSCYGHNFVKTMQSLFKVKESLSVINDQYGSPTWCEALAVAIFMLVKQTQATILTRPSVCE
DKPAKGLADLYHYAASASASWYEFACEIQRQAGSVTELAPSTKLSQNKEFAPNSADCSILPITTKAWQELHDNRLALRPK
QSALNTQKLCHQLAIEPGSLLTATWQQQLAAMVSYQKVKSRES

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=272, Percent_Identity=27.5735294117647, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI11034825, Length=214, Percent_Identity=28.9719626168224, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788352, Length=295, Percent_Identity=33.5593220338983, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 39884; Mature: 39884

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILITGAAGQLGQALLSIAGLTQVNLAERTVAQQMLVALLPEALECIETTDEVIGVSHQ
CEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ALDICDIDSIRKAFDTIAPDVVINCAAYNAVDKAEFDIDKAMLINAEGPKLLAGECQRHN
HCCCCCHHHHHHHHHHHCHHHEEEHHHHCCCCHHHHCCCCEEEEECCCCCEECCCCCCCC
IRLVHISTDFVFDGELLRAYTEQDSPAPLSVYGKSKLEGERWVSDILGSKATIIRTSWLY
EEEEEEECCEEECHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHH
SCYGHNFVKTMQSLFKVKESLSVINDQYGSPTWCEALAVAIFMLVKQTQATILTRPSVCE
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCC
DKPAKGLADLYHYAASASASWYEFACEIQRQAGSVTELAPSTKLSQNKEFAPNSADCSIL
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEE
PITTKAWQELHDNRLALRPKQSALNTQKLCHQLAIEPGSLLTATWQQQLAAMVSYQKVKS
ECCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
RES
CCC
>Mature Secondary Structure
MRILITGAAGQLGQALLSIAGLTQVNLAERTVAQQMLVALLPEALECIETTDEVIGVSHQ
CEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ALDICDIDSIRKAFDTIAPDVVINCAAYNAVDKAEFDIDKAMLINAEGPKLLAGECQRHN
HCCCCCHHHHHHHHHHHCHHHEEEHHHHCCCCHHHHCCCCEEEEECCCCCEECCCCCCCC
IRLVHISTDFVFDGELLRAYTEQDSPAPLSVYGKSKLEGERWVSDILGSKATIIRTSWLY
EEEEEEECCEEECHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHH
SCYGHNFVKTMQSLFKVKESLSVINDQYGSPTWCEALAVAIFMLVKQTQATILTRPSVCE
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCC
DKPAKGLADLYHYAASASASWYEFACEIQRQAGSVTELAPSTKLSQNKEFAPNSADCSIL
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEE
PITTKAWQELHDNRLALRPKQSALNTQKLCHQLAIEPGSLLTATWQQQLAAMVSYQKVKS
ECCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
RES
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]