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The map label for this gene is phoB [H]

Identifier: 157961258

GI number: 157961258

Start: 1740608

End: 1741831

Strand: Reverse

Name: phoB [H]

Synonym: Spea_1432

Alternate gene names: 157961258

Gene position: 1741831-1740608 (Counterclockwise)

Preceding gene: 157961264

Following gene: 157961257

Centisome position: 33.66

GC content: 44.85

Gene sequence:

>1224_bases
ATGCCTTTAACATTGAAGCTCATCAGCCAGCATAAAATCATCGTCGTAGAAGACTGCTATAGCGAGCGTTGTTTGCTACT
GACTTTACTCGAGTCAATGGGGTTTACGGCTCAAGGTTTTTCTAATGCCCAAGAGGCCATAGAACTGCTTCAGCGTGAGC
ATGTCGACATGGTGATCACCGATTGGATGATGCCTAAGATCAGCGGTATTGAGCTATGTAAAACTATAAAATCAATGCCT
TGCTCGCCATATACCATATTACTCACGGGTAATAGCCAGAATGCTCATCTAATAGAAGGGATCGAATCTGGCGCCGATGA
CTTTATTGCAAAGCCCTTTCATAGCGGCGTCCTGAAAGTAAGAATTCTTGCAGGCTTACGGATCATCGCGATGCAACAGA
AATTAGAAAGTCACAATCAAGCGCTCAATAATATGCTGCTAAAAGAGCAAGGTTACCTTAATAATCTTAAACATGACCTG
TCTCTCGCCGCTCAGCTGCAACGCGCTCTACTTCCCAAGAACTCAGATTTAAGCCAAGGCTGGCGAGTCACAACGGAATT
TAAACCAGCCCAAGATCTCGCAGGCGATCTGTTCCAATGCTTCAATATCGACAAGCAGCATATCGGCTTTTACCTATTGG
ATGTCAGCGGTCATGGTACGGCGGCATCGATGCTAAGCTTTACCCTAGCGCACCACTTATCTAGCAGCCAGCAAATTTGG
CAGTCACTTGATTTGGCACAGATAACTAACAAACTCAATCGAGAGTTTGACGACCCAGAGCAGTGTGGACGCTTTGCAAC
ACTACTTATTGGCATCGCAGACACTAACAGCGGAGAGGTTGAGATTATCAACGCTGGGCACCCCTCTCCTATCATAGTGG
GCCCACAAGGCGCTAGATTTGTCGATGATAACCACCTTGGATCACAGCTACCAATTGGCATCAACAAACAGCACAGTTAC
CACAGCCAACATTTCACCTTAGAGAAAGAAGAGCAGCTACTGCTCTATTCTGATGGCATTTATGAATGTCGCAACAGTAA
ATTTGGTAGTTTTGGCTTAGCAAAACTACTGAAAAATATCAGTGATGCCAGAGAGCTACCTGCAGAAAAGTTATTGCACT
ATCTCAGCTATAGCACTGAGCTTTGGCAAGAAAAACAAGCTCAGGACGATATCTCTCTGATGCTAATCTCAACCACAACT
CACTTAGCCCATTGTGATTCTTAA

Upstream 100 bases:

>100_bases
GAACCTAAAACAGAAATAAATATTTTCATTTGTCTGGCACAGAGTTTGCTTTATTACAAAGTACCCCTGAAACGACCGAT
AACAAGAGGAGGCTATGACA

Downstream 100 bases:

>100_bases
ATGTAAGGAAACACTATGAATAGCCTACAGTTTAACCTCGATAGAAATATTATGGCAAAACACTCCATAAGCAGAGAAAT
AGAGCAATTTATGCTGCAAA

Product: response regulator receiver modulated serine phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MPLTLKLISQHKIIVVEDCYSERCLLLTLLESMGFTAQGFSNAQEAIELLQREHVDMVITDWMMPKISGIELCKTIKSMP
CSPYTILLTGNSQNAHLIEGIESGADDFIAKPFHSGVLKVRILAGLRIIAMQQKLESHNQALNNMLLKEQGYLNNLKHDL
SLAAQLQRALLPKNSDLSQGWRVTTEFKPAQDLAGDLFQCFNIDKQHIGFYLLDVSGHGTAASMLSFTLAHHLSSSQQIW
QSLDLAQITNKLNREFDDPEQCGRFATLLIGIADTNSGEVEIINAGHPSPIIVGPQGARFVDDNHLGSQLPIGINKQHSY
HSQHFTLEKEEQLLLYSDGIYECRNSKFGSFGLAKLLKNISDARELPAEKLLHYLSYSTELWQEKQAQDDISLMLISTTT
HLAHCDS

Sequences:

>Translated_407_residues
MPLTLKLISQHKIIVVEDCYSERCLLLTLLESMGFTAQGFSNAQEAIELLQREHVDMVITDWMMPKISGIELCKTIKSMP
CSPYTILLTGNSQNAHLIEGIESGADDFIAKPFHSGVLKVRILAGLRIIAMQQKLESHNQALNNMLLKEQGYLNNLKHDL
SLAAQLQRALLPKNSDLSQGWRVTTEFKPAQDLAGDLFQCFNIDKQHIGFYLLDVSGHGTAASMLSFTLAHHLSSSQQIW
QSLDLAQITNKLNREFDDPEQCGRFATLLIGIADTNSGEVEIINAGHPSPIIVGPQGARFVDDNHLGSQLPIGINKQHSY
HSQHFTLEKEEQLLLYSDGIYECRNSKFGSFGLAKLLKNISDARELPAEKLLHYLSYSTELWQEKQAQDDISLMLISTTT
HLAHCDS
>Mature_406_residues
PLTLKLISQHKIIVVEDCYSERCLLLTLLESMGFTAQGFSNAQEAIELLQREHVDMVITDWMMPKISGIELCKTIKSMPC
SPYTILLTGNSQNAHLIEGIESGADDFIAKPFHSGVLKVRILAGLRIIAMQQKLESHNQALNNMLLKEQGYLNNLKHDLS
LAAQLQRALLPKNSDLSQGWRVTTEFKPAQDLAGDLFQCFNIDKQHIGFYLLDVSGHGTAASMLSFTLAHHLSSSQQIWQ
SLDLAQITNKLNREFDDPEQCGRFATLLIGIADTNSGEVEIINAGHPSPIIVGPQGARFVDDNHLGSQLPIGINKQHSYH
SQHFTLEKEEQLLLYSDGIYECRNSKFGSFGLAKLLKNISDARELPAEKLLHYLSYSTELWQEKQAQDDISLMLISTTTH
LAHCDS

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=35.8974358974359, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786599, Length=138, Percent_Identity=32.6086956521739, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=134, Percent_Identity=27.6119402985075, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=29.2682926829268, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788191, Length=115, Percent_Identity=29.5652173913043, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 45466; Mature: 45334

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTLKLISQHKIIVVEDCYSERCLLLTLLESMGFTAQGFSNAQEAIELLQREHVDMVIT
CCEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEE
DWMMPKISGIELCKTIKSMPCSPYTILLTGNSQNAHLIEGIESGADDFIAKPFHSGVLKV
HHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHCHHHCCCEEE
RILAGLRIIAMQQKLESHNQALNNMLLKEQGYLNNLKHDLSLAAQLQRALLPKNSDLSQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WRVTTEFKPAQDLAGDLFQCFNIDKQHIGFYLLDVSGHGTAASMLSFTLAHHLSSSQQIW
CEEEECCCCHHHHHHHHHHHHCCCCHHCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHH
QSLDLAQITNKLNREFDDPEQCGRFATLLIGIADTNSGEVEIINAGHPSPIIVGPQGARF
HHCCHHHHHHHHCCCCCCHHHHHHHEEHEEEEECCCCCCEEEEECCCCCCEEECCCCCEE
VDDNHLGSQLPIGINKQHSYHSQHFTLEKEEQLLLYSDGIYECRNSKFGSFGLAKLLKNI
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCHHHHCCCCCCCHHHHHHHHHH
SDARELPAEKLLHYLSYSTELWQEKQAQDDISLMLISTTTHLAHCDS
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCC
>Mature Secondary Structure 
PLTLKLISQHKIIVVEDCYSERCLLLTLLESMGFTAQGFSNAQEAIELLQREHVDMVIT
CEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEE
DWMMPKISGIELCKTIKSMPCSPYTILLTGNSQNAHLIEGIESGADDFIAKPFHSGVLKV
HHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHCHHHCCCEEE
RILAGLRIIAMQQKLESHNQALNNMLLKEQGYLNNLKHDLSLAAQLQRALLPKNSDLSQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WRVTTEFKPAQDLAGDLFQCFNIDKQHIGFYLLDVSGHGTAASMLSFTLAHHLSSSQQIW
CEEEECCCCHHHHHHHHHHHHCCCCHHCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHH
QSLDLAQITNKLNREFDDPEQCGRFATLLIGIADTNSGEVEIINAGHPSPIIVGPQGARF
HHCCHHHHHHHHCCCCCCHHHHHHHEEHEEEEECCCCCCEEEEECCCCCCEEECCCCCEE
VDDNHLGSQLPIGINKQHSYHSQHFTLEKEEQLLLYSDGIYECRNSKFGSFGLAKLLKNI
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCHHHHCCCCCCCHHHHHHHHHH
SDARELPAEKLLHYLSYSTELWQEKQAQDDISLMLISTTTHLAHCDS
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]