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The map label for this gene is rfbB [C]

Identifier: 157961245

GI number: 157961245

Start: 1724390

End: 1725400

Strand: Direct

Name: rfbB [C]

Synonym: Spea_1419

Alternate gene names: 157961245

Gene position: 1724390-1725400 (Clockwise)

Preceding gene: 157961244

Following gene: 157961246

Centisome position: 33.32

GC content: 42.04

Gene sequence:

>1011_bases
ATGAAGTATTTAGTCACAGGTGCGGCAGGTTTTATTGGCGCAAAAGTGAGTGAGCGCTTATGTGCTGCTGGTCATGAAGT
GGTTGGTATCGACAATATTAACGACTATTACGATGTTAACTTAAAACTGGACCGCCTTAAAAATTTGGAGCCTCTGGCTC
TTTTTTCATTTAAAAAATTAGACTTAGCAGATAGAGAGGGCATTGCCGCACTATTTGCTGAGCAAAGTTTTGATCGAGTG
ATTCATTTAGCCGCTCAAGCCGGAGTTCGTTATTCTATTGATAACCCAATGGCCTATGCCGACAGTAACTTGGTCGGGCA
TTTGACTATATTAGAAGGCTGTCGCCACCATAAGATTCAGCATTTAGTCTACGCTTCATCTAGCTCTGTTTATGGCCTTA
ACTCTAAAATGCCATTCTCAACTGATGATAGTGTGGATCATCCTATCTCTCTATATGCGGCGACTAAGAAAGCCAATGAG
CTGATGTCACATACCTATTCTCATCTTTATAGTGTGCCAACGACTGGCTTGCGTTTCTTTACTGTTTACGGCCCTTGGAG
CCGACCAGATATGGCACTGCTCAAGTTCACCAATAAGATAGTAAAAGGTGAGGCCATTGATGTTTATAACCACGGTAATT
TGAGCCGAGATTTTACCTATATCGATGATATCGTTGAAGGCATCATTCGTATTCAAGATAGTATTCCAAGTGCTAATGCA
GAATGGAATGCGGCTGAGGCAACACCAGCAACAAGCTCTGCACCGTATCGCGTATTTAACATAGGTAACGGCAGTCCGGT
TAAGCTGATGGATTATATCTCTGCACTTGAGAAATCATTGGGGATTGAAGCCATTAAAAACATGATGGACATGCAACCAG
GTGACGTGCATTCAACATGGGCTGATACTGAAGACTTGTTTAAGACTGTTGGTTACAAGCCACAAACTAGCGTCGAAGAA
GGCGTACAAAAGTTTGTTGAATGGTATAAAGAATATTACATGAAAGGCTGA

Upstream 100 bases:

>100_bases
GTTGGTCGGCATTTATGTTGTCGTTTCTTTTGATTTTGTTTTCCTAGCAGGCCAGAAGCCTTCCTAGAATCTAGGGTCTT
CTTTAACAAAAGAGAATTTT

Downstream 100 bases:

>100_bases
GAAGCCCCTAGGTTAAAGCAAAGACGGTATAAGCTAAGACGGACAAGCTGAATTACGGTATACGGAACGTTCGCAGGCTC
ACTCACGGAACGCTGCGCTG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKYLVTGAAGFIGAKVSERLCAAGHEVVGIDNINDYYDVNLKLDRLKNLEPLALFSFKKLDLADREGIAALFAEQSFDRV
IHLAAQAGVRYSIDNPMAYADSNLVGHLTILEGCRHHKIQHLVYASSSSVYGLNSKMPFSTDDSVDHPISLYAATKKANE
LMSHTYSHLYSVPTTGLRFFTVYGPWSRPDMALLKFTNKIVKGEAIDVYNHGNLSRDFTYIDDIVEGIIRIQDSIPSANA
EWNAAEATPATSSAPYRVFNIGNGSPVKLMDYISALEKSLGIEAIKNMMDMQPGDVHSTWADTEDLFKTVGYKPQTSVEE
GVQKFVEWYKEYYMKG

Sequences:

>Translated_336_residues
MKYLVTGAAGFIGAKVSERLCAAGHEVVGIDNINDYYDVNLKLDRLKNLEPLALFSFKKLDLADREGIAALFAEQSFDRV
IHLAAQAGVRYSIDNPMAYADSNLVGHLTILEGCRHHKIQHLVYASSSSVYGLNSKMPFSTDDSVDHPISLYAATKKANE
LMSHTYSHLYSVPTTGLRFFTVYGPWSRPDMALLKFTNKIVKGEAIDVYNHGNLSRDFTYIDDIVEGIIRIQDSIPSANA
EWNAAEATPATSSAPYRVFNIGNGSPVKLMDYISALEKSLGIEAIKNMMDMQPGDVHSTWADTEDLFKTVGYKPQTSVEE
GVQKFVEWYKEYYMKG
>Mature_336_residues
MKYLVTGAAGFIGAKVSERLCAAGHEVVGIDNINDYYDVNLKLDRLKNLEPLALFSFKKLDLADREGIAALFAEQSFDRV
IHLAAQAGVRYSIDNPMAYADSNLVGHLTILEGCRHHKIQHLVYASSSSVYGLNSKMPFSTDDSVDHPISLYAATKKANE
LMSHTYSHLYSVPTTGLRFFTVYGPWSRPDMALLKFTNKIVKGEAIDVYNHGNLSRDFTYIDDIVEGIIRIQDSIPSANA
EWNAAEATPATSSAPYRVFNIGNGSPVKLMDYISALEKSLGIEAIKNMMDMQPGDVHSTWADTEDLFKTVGYKPQTSVEE
GVQKFVEWYKEYYMKG

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=347, Percent_Identity=26.5129682997118, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56237023, Length=352, Percent_Identity=24.4318181818182, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI56118217, Length=352, Percent_Identity=24.4318181818182, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI189083684, Length=352, Percent_Identity=24.4318181818182, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=23.7388724035608, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1788353, Length=369, Percent_Identity=27.9132791327913, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=26.2430939226519, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=21.0826210826211, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=25.5681818181818, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=25.1412429378531, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=22.8486646884273, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=233, Percent_Identity=26.6094420600858, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=361, Percent_Identity=24.9307479224377, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19923002, Length=350, Percent_Identity=25.4285714285714, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=23.2142857142857, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37422; Mature: 37422

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLVTGAAGFIGAKVSERLCAAGHEVVGIDNINDYYDVNLKLDRLKNLEPLALFSFKKL
CCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEHHHHCCCCCHHHHEEHHC
DLADREGIAALFAEQSFDRVIHLAAQAGVRYSIDNPMAYADSNLVGHLTILEGCRHHKIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCCEEEHHHHHHHHHHHHH
HLVYASSSSVYGLNSKMPFSTDDSVDHPISLYAATKKANELMSHTYSHLYSVPTTGLRFF
HEEEECCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWSRPDMALLKFTNKIVKGEAIDVYNHGNLSRDFTYIDDIVEGIIRIQDSIPSANA
EEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
EWNAAEATPATSSAPYRVFNIGNGSPVKLMDYISALEKSLGIEAIKNMMDMQPGDVHSTW
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
ADTEDLFKTVGYKPQTSVEEGVQKFVEWYKEYYMKG
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYLVTGAAGFIGAKVSERLCAAGHEVVGIDNINDYYDVNLKLDRLKNLEPLALFSFKKL
CCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEHHHHCCCCCHHHHEEHHC
DLADREGIAALFAEQSFDRVIHLAAQAGVRYSIDNPMAYADSNLVGHLTILEGCRHHKIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCCEEEHHHHHHHHHHHHH
HLVYASSSSVYGLNSKMPFSTDDSVDHPISLYAATKKANELMSHTYSHLYSVPTTGLRFF
HEEEECCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWSRPDMALLKFTNKIVKGEAIDVYNHGNLSRDFTYIDDIVEGIIRIQDSIPSANA
EEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
EWNAAEATPATSSAPYRVFNIGNGSPVKLMDYISALEKSLGIEAIKNMMDMQPGDVHSTW
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
ADTEDLFKTVGYKPQTSVEEGVQKFVEWYKEYYMKG
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]