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The map label for this gene is fliA [H]

Identifier: 157961206

GI number: 157961206

Start: 1675741

End: 1676460

Strand: Direct

Name: fliA [H]

Synonym: Spea_1378

Alternate gene names: 157961206

Gene position: 1675741-1676460 (Clockwise)

Preceding gene: 157961205

Following gene: 157961207

Centisome position: 32.38

GC content: 46.67

Gene sequence:

>720_bases
GTGAATAAAGCGGCTGCGTATACTCGTTTTGATAATAAGACGTCTATCGTTGAACAGTATGCACCGCTTGTAAAAAGAAT
AGCCCATCATCTTTTGGCTCGTTTACCTGCATCAGTCCAGTTAGATGACTTGTTGCAGGCCGGCATGATGGGGCTACTCG
AAGCGTCATCAAACTTTGATGGTAGTAAGGGCGCCAAATTCGAAACCTTTGCCGGTATTCGAATTCGGGGCTCTATGTTA
GATGAAATCAGGCGAGGCGACTGGGTACCCCGTTCGGTACACCGTAATCAGCGCCGCGTGGCCCAAGTTATAGATGAACT
TGAGCAGGAGTTAGGCCGTGATGCTCGTGATGGCGAAATCGCCGAGCGACTCGAGGTTTCACTCGAAGAGTATCATGCAA
TTTTAAACGATGTTTCAGTCGGAAAAGTTGTTGGTATAGAAGATTTAGGCGTAGCCGCAGATGGGATAAACCCAGAAGAT
GGCCACGAAGACGATGCTTATGAATCACTCGCTGAAGTTGAGTTTCATTCAGCGCTCGTCGCAGCGATTAAATTATTACC
AGAAAGAGATGCGCTGGTTTTGTCGCTTTATTACGATGAAGCATTAAATTTAAAAGAGATTGGGGCCATTCTTGAGGTCA
GTGAGTCTAGAGTCAGCCAGATCCATAGTCAGGCGATGCTCAGGCTCAAGGGTAAACTCAAGCATTGGACGCAAGAATAA

Upstream 100 bases:

>100_bases
AAGATCATGAGCTGGCCAGTGCCGTCTCAGCCAGGCGGTCATTTAGAGTTTTTCGTTGAGCGTCTAGTTCAGCGTCCTGA
TTATCAAGAGGATAAATCGG

Downstream 100 bases:

>100_bases
AATCATAATCATTGATAGCACATTACACGCGCATATATGGCGCGCTCCGCGGAGGAAACCTTGGACAAGAATATGAAGAT
TCTTGTTGTTGACGATTTTT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFDGSKGAKFETFAGIRIRGSML
DEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEIAERLEVSLEEYHAILNDVSVGKVVGIEDLGVAADGINPED
GHEDDAYESLAEVEFHSALVAAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE

Sequences:

>Translated_239_residues
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFDGSKGAKFETFAGIRIRGSML
DEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEIAERLEVSLEEYHAILNDVSVGKVVGIEDLGVAADGINPED
GHEDDAYESLAEVEFHSALVAAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE
>Mature_239_residues
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFDGSKGAKFETFAGIRIRGSML
DEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEIAERLEVSLEEYHAILNDVSVGKVVGIEDLGVAADGINPED
GHEDDAYESLAEVEFHSALVAAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=225, Percent_Identity=49.7777777777778, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=25.6410256410256, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26625; Mature: 26625

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFD
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
GSKGAKFETFAGIRIRGSMLDEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEI
CCCCCCEEHCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AERLEVSLEEYHAILNDVSVGKVVGIEDLGVAADGINPEDGHEDDAYESLAEVEFHSALV
HHHHHHHHHHHHHHHHCCCCCCEECHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKAAAYTRFDNKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSNFD
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
GSKGAKFETFAGIRIRGSMLDEIRRGDWVPRSVHRNQRRVAQVIDELEQELGRDARDGEI
CCCCCCEEHCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AERLEVSLEEYHAILNDVSVGKVVGIEDLGVAADGINPEDGHEDDAYESLAEVEFHSALV
HHHHHHHHHHHHHHHHCCCCCCEECHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQIHSQAMLRLKGKLKHWTQE
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]