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The map label for this gene is mltF

Identifier: 157961144

GI number: 157961144

Start: 1603486

End: 1604925

Strand: Reverse

Name: mltF

Synonym: Spea_1316

Alternate gene names: 157961144

Gene position: 1604925-1603486 (Counterclockwise)

Preceding gene: 157961149

Following gene: 157961140

Centisome position: 31.02

GC content: 48.96

Gene sequence:

>1440_bases
ATGAAAAGACTCCTGCTTGTTCTATGCTATATCACCCTACTGGCTGGCTGCCAGAAGGTTGTCGTGGAGCAAGAAAAAAC
CACTCCCCCATTAAAGCCGACTCAATTAATTGTCGGTACTTTATATGGTCCACAAATTTACTTTACCTCAGGGCAAGGGG
ACTCTGGTTATGACTATGAGATGGCCGAGCGCTTCGCCAATTACCTCGATCTCGAGTTAAAGATGAAGCCTTTCGCCAAT
ATCAGCGAGCTTTATAGCGCGATGCATAGTGGAGAAATCGATATTATTGCCGCGGGTTTAGCCGATACGCCTGCAAGACG
CGAGCAGTTTAGACTCGGCCCGCCACTCTATCGAGTTAACCAAGTGTTGGTTTATCGCCAAGGTACACCCATACCTAGAA
GTGTCGACACACTTAAAGGCGAAATCACGGTAACCACTGACTCCTCTTTTGTCGACACCTTAACAGAGCTGCAAAAGTCC
AATCCGGATCTGGTGTGGAATCAGGAAAAAGATAAGGACGCCGAAGAGCTACTCACCATGATTGCCGCCGGAGAGATCCC
CTACACTATCGCCGACTCGACCAGTTTAGACATCAACAGACGTTTTATGCCTGAGCTACGAGAAGGCCTTGTTCTAAAAC
GTAAACAGCCAGTTGTATGGTTATTACCACCCACCAACAGCGACAAGCTGATGAGTGAGCTATTGTCGTTTTGGCATATA
GAAAAACGCAGTGGCACCTTGGCTCACCTCAATGAGAAATACTTTGCTCACGTTGAGCGTTTCGATTATGTCGACACCCG
CGCCTTTATTCGTGCCATCGATAACAAGCTACCTAAATATCAAGCCACGTTTGAAAAATATGCCGAGGGCATAGACTGGC
GTAAGCTTGCCGCAACCGCATACCAGGAGTCCCATTGGAACCCTAATGCTCGTTCACCGACGGGTGTGCGCGGTTTGATG
ATGTTGACCCTGCCAACCGCAAAACAAGTTGGTATCAAAAACCGTCTCGATCCTTACCAGAGCATCAAGGGCGGCGCAAA
GTACCTCAACAGCATGCTTGAGCGTCTGCCAGATTCCATCCCAGAGAGTCAGCGTATGTGGTTCGCCCTCGCCTCCTACA
ACATAGGCTTAGGTCATGTGGAAGATGCGCGCAAGCTGGCACAGTCTCAAGGATTGAACCCGAGTGCTTGGCGAGATGTG
AAATCGGTATTACCACTGCTGCAAAAGCGAAAGTACTACCAAAAAACCCGCTATGGTTACGCAAGAGGCAATGAGGCGGT
GCATTACGTCGATAGTATTCGTCGTTACTATGACACGCTAGTGTGGATCGATAATCAGAATATGCTGCTTGAGCTCAAAC
AGAAGCCGCTTCAAACCGCCGAGGCTAAAGAGACTGAAGAGAAACCCCAGACTGACGCAATCCAGCCGCAACAGCCTTAA

Upstream 100 bases:

>100_bases
AATACTTTTCAATAAAAATTCAGTTTTTTTACGCCAAGCAAGATTGTGGTATAATCGACGACAATTTCGTTAAAATTTAA
GTGCTAATAAAAAATAATCG

Downstream 100 bases:

>100_bases
GCAAGGTCTGATTATAGATAGCCTTGAGCTTAGCCCGATTATAGACAGCCTTGAGGTTTTAAAAATAGCTATACAACCTT
GATCCAATTGAATAGGCCAT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKRLLLVLCYITLLAGCQKVVVEQEKTTPPLKPTQLIVGTLYGPQIYFTSGQGDSGYDYEMAERFANYLDLELKMKPFAN
ISELYSAMHSGEIDIIAAGLADTPARREQFRLGPPLYRVNQVLVYRQGTPIPRSVDTLKGEITVTTDSSFVDTLTELQKS
NPDLVWNQEKDKDAEELLTMIAAGEIPYTIADSTSLDINRRFMPELREGLVLKRKQPVVWLLPPTNSDKLMSELLSFWHI
EKRSGTLAHLNEKYFAHVERFDYVDTRAFIRAIDNKLPKYQATFEKYAEGIDWRKLAATAYQESHWNPNARSPTGVRGLM
MLTLPTAKQVGIKNRLDPYQSIKGGAKYLNSMLERLPDSIPESQRMWFALASYNIGLGHVEDARKLAQSQGLNPSAWRDV
KSVLPLLQKRKYYQKTRYGYARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKQKPLQTAEAKETEEKPQTDAIQPQQP

Sequences:

>Translated_479_residues
MKRLLLVLCYITLLAGCQKVVVEQEKTTPPLKPTQLIVGTLYGPQIYFTSGQGDSGYDYEMAERFANYLDLELKMKPFAN
ISELYSAMHSGEIDIIAAGLADTPARREQFRLGPPLYRVNQVLVYRQGTPIPRSVDTLKGEITVTTDSSFVDTLTELQKS
NPDLVWNQEKDKDAEELLTMIAAGEIPYTIADSTSLDINRRFMPELREGLVLKRKQPVVWLLPPTNSDKLMSELLSFWHI
EKRSGTLAHLNEKYFAHVERFDYVDTRAFIRAIDNKLPKYQATFEKYAEGIDWRKLAATAYQESHWNPNARSPTGVRGLM
MLTLPTAKQVGIKNRLDPYQSIKGGAKYLNSMLERLPDSIPESQRMWFALASYNIGLGHVEDARKLAQSQGLNPSAWRDV
KSVLPLLQKRKYYQKTRYGYARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKQKPLQTAEAKETEEKPQTDAIQPQQP
>Mature_479_residues
MKRLLLVLCYITLLAGCQKVVVEQEKTTPPLKPTQLIVGTLYGPQIYFTSGQGDSGYDYEMAERFANYLDLELKMKPFAN
ISELYSAMHSGEIDIIAAGLADTPARREQFRLGPPLYRVNQVLVYRQGTPIPRSVDTLKGEITVTTDSSFVDTLTELQKS
NPDLVWNQEKDKDAEELLTMIAAGEIPYTIADSTSLDINRRFMPELREGLVLKRKQPVVWLLPPTNSDKLMSELLSFWHI
EKRSGTLAHLNEKYFAHVERFDYVDTRAFIRAIDNKLPKYQATFEKYAEGIDWRKLAATAYQESHWNPNARSPTGVRGLM
MLTLPTAKQVGIKNRLDPYQSIKGGAKYLNSMLERLPDSIPESQRMWFALASYNIGLGHVEDARKLAQSQGLNPSAWRDV
KSVLPLLQKRKYYQKTRYGYARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKQKPLQTAEAKETEEKPQTDAIQPQQP

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=409, Percent_Identity=45.2322738386308, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_SHEPA (A8H256)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501178.1
- ProteinModelPortal:   A8H256
- STRING:   A8H256
- GeneID:   5661715
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1316
- NMPDR:   fig|398579.3.peg.1138
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- OMA:   QYVENIR
- ProtClustDB:   PRK10859
- BioCyc:   SPEA398579:SPEA_1316-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 54808; Mature: 54808

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00922 TRANSGLYCOSYLASE; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLLVLCYITLLAGCQKVVVEQEKTTPPLKPTQLIVGTLYGPQIYFTSGQGDSGYDYE
CHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHEEEEEEECCEEEEECCCCCCCCCHH
MAERFANYLDLELKMKPFANISELYSAMHSGEIDIIAAGLADTPARREQFRLGPPLYRVN
HHHHHHHHHCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCCHHHHC
QVLVYRQGTPIPRSVDTLKGEITVTTDSSFVDTLTELQKSNPDLVWNQEKDKDAEELLTM
EEEEEECCCCCCCCHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHH
IAAGEIPYTIADSTSLDINRRFMPELREGLVLKRKQPVVWLLPPTNSDKLMSELLSFWHI
HHCCCCCEEEECCCCCCCCHHHHHHHHHCCEEECCCCEEEEECCCCHHHHHHHHHHHHHH
EKRSGTLAHLNEKYFAHVERFDYVDTRAFIRAIDNKLPKYQATFEKYAEGIDWRKLAATA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
YQESHWNPNARSPTGVRGLMMLTLPTAKQVGIKNRLDPYQSIKGGAKYLNSMLERLPDSI
HHHHCCCCCCCCCCCCCEEEEEEECCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCC
PESQRMWFALASYNIGLGHVEDARKLAQSQGLNPSAWRDVKSVLPLLQKRKYYQKTRYGY
CCHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKQKPLQTAEAKETEEKPQTDAIQPQQP
CCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKRLLLVLCYITLLAGCQKVVVEQEKTTPPLKPTQLIVGTLYGPQIYFTSGQGDSGYDYE
CHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHEEEEEEECCEEEEECCCCCCCCCHH
MAERFANYLDLELKMKPFANISELYSAMHSGEIDIIAAGLADTPARREQFRLGPPLYRVN
HHHHHHHHHCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCCHHHHC
QVLVYRQGTPIPRSVDTLKGEITVTTDSSFVDTLTELQKSNPDLVWNQEKDKDAEELLTM
EEEEEECCCCCCCCHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHH
IAAGEIPYTIADSTSLDINRRFMPELREGLVLKRKQPVVWLLPPTNSDKLMSELLSFWHI
HHCCCCCEEEECCCCCCCCHHHHHHHHHCCEEECCCCEEEEECCCCHHHHHHHHHHHHHH
EKRSGTLAHLNEKYFAHVERFDYVDTRAFIRAIDNKLPKYQATFEKYAEGIDWRKLAATA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
YQESHWNPNARSPTGVRGLMMLTLPTAKQVGIKNRLDPYQSIKGGAKYLNSMLERLPDSI
HHHHCCCCCCCCCCCCCEEEEEEECCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCC
PESQRMWFALASYNIGLGHVEDARKLAQSQGLNPSAWRDVKSVLPLLQKRKYYQKTRYGY
CCHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARGNEAVHYVDSIRRYYDTLVWIDNQNMLLELKQKPLQTAEAKETEEKPQTDAIQPQQP
CCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA