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The map label for this gene is hprA [H]

Identifier: 157960698

GI number: 157960698

Start: 1069095

End: 1070048

Strand: Direct

Name: hprA [H]

Synonym: Spea_0870

Alternate gene names: 157960698

Gene position: 1069095-1070048 (Clockwise)

Preceding gene: 157960697

Following gene: 157960700

Centisome position: 20.66

GC content: 47.27

Gene sequence:

>954_bases
ATGAAACTTGTTGTGCTCGACGGATATACGTTAAATCCAGGTGATCTCGATTGGCAAGCATTAGCAGCGTTTGGAGAGCT
AAGCGTCTTCGAACGTAGCGCGCCTGAAAAGGTACTCATCCGTAGTCAGGATGCAGAGATTATCTTTACCAATAAGACAG
TGCTCGATGCCGCAACATTAAGGCAACTGCCTAAGCTTAAGTATATCGGCGTCCTTGCTACAGGCACCAATGTGGTCGAT
CTCGAGACTGCAAAAGAGCTGGGAATAGTGGTCACCAATGTGCCCGGTTACGGTCCCGATGCGGTCGCACAGATGGTGTT
TGCTCATATACTCCATGCCACGCAGCAGGTTGCTTTGCACTCTGACGCTGTAAAGCGCGGGATCTGGAGTGAGTCGAAAG
ATTTTTGCTTTACACTTACGCCGCTGCAATCGTTAAAGGGCAAACGTTTAGGTTTAATCGGTTTTGGTGATATAGGTAAA
GTGGTTGCTCAAATTGGGCTGGCCTTTGGTATGGCGGTATTGGTCAATACCCGTAATCCTAAAATCAAATTGCCAGAGGG
TTGCCGTTGGGCCGAGCGAGAGGTGTTATTTAGTCAGTCAGATATTATTTCTCTGCACTGTCCGTTCACCCCTGATACCG
ATAAATTGATCGATGCTAGCGTGCTTGAGAAGATGAAGTCTTCAGCAATCTTGATTAACACCGCTCGTGGTGGTCTTATC
GATGAAGCTGCATTGTCGGCTGCATTAGAGCTAGGAGCGATAGCTTTTGCGGGTGTTGATGTATTGTCTACCGAGCCACC
ATGCCAAGATAACCCATTGCTGAGAGCCAAGAATATCAGTATCTCGCCCCATAACGCCTGGGCAACCATAGAAGCTAGGC
AGAACTTGCTCAATATAGCAGTCGATAATCTTTCGAGTTTTAGTCAAGGCCAAGTGAAAAACCGAGTTAATTGA

Upstream 100 bases:

>100_bases
TAAGTTCTAAGCGCTAATTTTTAGGGACAAGGTTTAGAGCCAACTTTTAGAGCTAAGTTTACGTTTTGGAGTTAGTGATT
TTTTAGCTAAGGTTATTTTG

Downstream 100 bases:

>100_bases
TTTCCTGATATTCTGGTGAAATTTAGCTAGAAGGTATAAAACAAATAAGCCTCAAATTTAACGCTAAACCTTTAGCGAAA
ATTTGAGGCTTTTTAATTAG

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKLVVLDGYTLNPGDLDWQALAAFGELSVFERSAPEKVLIRSQDAEIIFTNKTVLDAATLRQLPKLKYIGVLATGTNVVD
LETAKELGIVVTNVPGYGPDAVAQMVFAHILHATQQVALHSDAVKRGIWSESKDFCFTLTPLQSLKGKRLGLIGFGDIGK
VVAQIGLAFGMAVLVNTRNPKIKLPEGCRWAEREVLFSQSDIISLHCPFTPDTDKLIDASVLEKMKSSAILINTARGGLI
DEAALSAALELGAIAFAGVDVLSTEPPCQDNPLLRAKNISISPHNAWATIEARQNLLNIAVDNLSSFSQGQVKNRVN

Sequences:

>Translated_317_residues
MKLVVLDGYTLNPGDLDWQALAAFGELSVFERSAPEKVLIRSQDAEIIFTNKTVLDAATLRQLPKLKYIGVLATGTNVVD
LETAKELGIVVTNVPGYGPDAVAQMVFAHILHATQQVALHSDAVKRGIWSESKDFCFTLTPLQSLKGKRLGLIGFGDIGK
VVAQIGLAFGMAVLVNTRNPKIKLPEGCRWAEREVLFSQSDIISLHCPFTPDTDKLIDASVLEKMKSSAILINTARGGLI
DEAALSAALELGAIAFAGVDVLSTEPPCQDNPLLRAKNISISPHNAWATIEARQNLLNIAVDNLSSFSQGQVKNRVN
>Mature_317_residues
MKLVVLDGYTLNPGDLDWQALAAFGELSVFERSAPEKVLIRSQDAEIIFTNKTVLDAATLRQLPKLKYIGVLATGTNVVD
LETAKELGIVVTNVPGYGPDAVAQMVFAHILHATQQVALHSDAVKRGIWSESKDFCFTLTPLQSLKGKRLGLIGFGDIGK
VVAQIGLAFGMAVLVNTRNPKIKLPEGCRWAEREVLFSQSDIISLHCPFTPDTDKLIDASVLEKMKSSAILINTARGGLI
DEAALSAALELGAIAFAGVDVLSTEPPCQDNPLLRAKNISISPHNAWATIEARQNLLNIAVDNLSSFSQGQVKNRVN

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=274, Percent_Identity=32.1167883211679, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI145580578, Length=295, Percent_Identity=31.1864406779661, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4557499, Length=295, Percent_Identity=31.1864406779661, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4557497, Length=272, Percent_Identity=32.3529411764706, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI145580575, Length=272, Percent_Identity=32.7205882352941, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI61743967, Length=272, Percent_Identity=32.3529411764706, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI6912396, Length=267, Percent_Identity=31.8352059925094, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI87082289, Length=280, Percent_Identity=35, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787645, Length=336, Percent_Identity=33.0357142857143, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=31.6602316602317, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081824, Length=140, Percent_Identity=33.5714285714286, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=255, Percent_Identity=30.1960784313725, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25147481, Length=283, Percent_Identity=30.3886925795053, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=314, Percent_Identity=29.2993630573248, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=282, Percent_Identity=30.4964539007092, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=236, Percent_Identity=30.5084745762712, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=304, Percent_Identity=27.3026315789474, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28571528, Length=257, Percent_Identity=35.0194552529183, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646446, Length=261, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646448, Length=261, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646452, Length=261, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24646450, Length=261, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI62472511, Length=295, Percent_Identity=31.5254237288136, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28574286, Length=258, Percent_Identity=29.8449612403101, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45552429, Length=258, Percent_Identity=27.906976744186, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28574284, Length=258, Percent_Identity=27.906976744186, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585514, Length=258, Percent_Identity=27.906976744186, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28574282, Length=258, Percent_Identity=27.906976744186, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45551003, Length=258, Percent_Identity=27.906976744186, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585516, Length=266, Percent_Identity=27.0676691729323, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921140, Length=274, Percent_Identity=28.1021897810219, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34181; Mature: 34181

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVVLDGYTLNPGDLDWQALAAFGELSVFERSAPEKVLIRSQDAEIIFTNKTVLDAATL
CEEEEEECEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHH
RQLPKLKYIGVLATGTNVVDLETAKELGIVVTNVPGYGPDAVAQMVFAHILHATQQVALH
HHCCCEEEEEEEECCCCEEEECCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SDAVKRGIWSESKDFCFTLTPLQSLKGKRLGLIGFGDIGKVVAQIGLAFGMAVLVNTRNP
HHHHHHCCCCCCCCEEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHHHCEEEEEECCCC
KIKLPEGCRWAEREVLFSQSDIISLHCPFTPDTDKLIDASVLEKMKSSAILINTARGGLI
CEECCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCH
DEAALSAALELGAIAFAGVDVLSTEPPCQDNPLLRAKNISISPHNAWATIEARQNLLNIA
HHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHH
VDNLSSFSQGQVKNRVN
HHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MKLVVLDGYTLNPGDLDWQALAAFGELSVFERSAPEKVLIRSQDAEIIFTNKTVLDAATL
CEEEEEECEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHH
RQLPKLKYIGVLATGTNVVDLETAKELGIVVTNVPGYGPDAVAQMVFAHILHATQQVALH
HHCCCEEEEEEEECCCCEEEECCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SDAVKRGIWSESKDFCFTLTPLQSLKGKRLGLIGFGDIGKVVAQIGLAFGMAVLVNTRNP
HHHHHHCCCCCCCCEEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHHHCEEEEEECCCC
KIKLPEGCRWAEREVLFSQSDIISLHCPFTPDTDKLIDASVLEKMKSSAILINTARGGLI
CEECCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCH
DEAALSAALELGAIAFAGVDVLSTEPPCQDNPLLRAKNISISPHNAWATIEARQNLLNIA
HHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHH
VDNLSSFSQGQVKNRVN
HHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]