Click here to switch to the map view.

The map label for this gene is dhfrIII [H]

Identifier: 157960685

GI number: 157960685

Start: 1055458

End: 1055940

Strand: Direct

Name: dhfrIII [H]

Synonym: Spea_0857

Alternate gene names: 157960685

Gene position: 1055458-1055940 (Clockwise)

Preceding gene: 157960684

Following gene: 157960686

Centisome position: 20.4

GC content: 41.61

Gene sequence:

>483_bases
ATGAAAATAGCTATGATTGCCGCGATGGCAAATAACCGCGTTATTGGTAAAGATAATCAAATGCCATGGCATCTCCCAGA
AGATCTACGACACTTCAAAGCTATGACCTTAGGTAAGCCTGTAGTTATGGGAAGAAAGACCTATGAGTCGATTGGTCGTC
CTTTACCTGGTCGTCATAATATCGTCATCAGTCGTCAAGATAGTTTGGTTATTGATGGGGTAACAAGGGTTAGCAGCTTT
GAGGAGGCTAAGATAGCCGCTGGCGATTGTGATGAGTTAGTGGTGATGGGCGGTGGTCAGCTGTATGAGATGTTACTTTC
TCAGGCTGATATCTTATATCTCACTGAAATTAATTTAACTGTCGAGGGTGATACCTATTTTCCTGAGTGGGATAATGGTA
GCTGGCAAGAAGTCTCTCGGGATGTTGCTAAAAATGACAAAGATGTTGAATACAGCTTTATCAAGTTGGTAAAAAAGTGT
TAA

Upstream 100 bases:

>100_bases
CCGCTCACGCCCAGTCATCAAATAATTTAACTTTATCAAGCCTACAGGGCAGTTGTGTTAAATTTGGCTTCACATTAGAC
CTATATAAAAGGGAAGTATC

Downstream 100 bases:

>100_bases
ATTCATAGGGTTGTTTAGTACCCTACATAAAGATAAAGACAGCGACTAAACAGCGCTACTTTTCTATAAATAAAAACTAA
AAATAAGGAGTGACCGATGC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHNIVISRQDSLVIDGVTRVSSF
EEAKIAAGDCDELVVMGGGQLYEMLLSQADILYLTEINLTVEGDTYFPEWDNGSWQEVSRDVAKNDKDVEYSFIKLVKKC

Sequences:

>Translated_160_residues
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHNIVISRQDSLVIDGVTRVSSF
EEAKIAAGDCDELVVMGGGQLYEMLLSQADILYLTEINLTVEGDTYFPEWDNGSWQEVSRDVAKNDKDVEYSFIKLVKKC
>Mature_160_residues
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHNIVISRQDSLVIDGVTRVSSF
EEAKIAAGDCDELVVMGGGQLYEMLLSQADILYLTEINLTVEGDTYFPEWDNGSWQEVSRDVAKNDKDVEYSFIKLVKKC

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=151, Percent_Identity=50.9933774834437, Blast_Score=153, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17505899, Length=156, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324810, Length=136, Percent_Identity=31.6176470588235, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116008026, Length=158, Percent_Identity=33.5443037974684, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24647458, Length=158, Percent_Identity=33.5443037974684, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18021; Mature: 18021

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHN
CCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVISRQDSLVIDGVTRVSSFEEAKIAAGDCDELVVMGGGQLYEMLLSQADILYLTEINLT
EEEECCCCEEEECHHHHCCCCCCEECCCCCCEEEEECCHHHHHHHHCCCCEEEEEEEEEE
VEGDTYFPEWDNGSWQEVSRDVAKNDKDVEYSFIKLVKKC
EECCEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHN
CCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVISRQDSLVIDGVTRVSSFEEAKIAAGDCDELVVMGGGQLYEMLLSQADILYLTEINLT
EEEECCCCEEEECHHHHCCCCCCEECCCCCCEEEEECCHHHHHHHHCCCCEEEEEEEEEE
VEGDTYFPEWDNGSWQEVSRDVAKNDKDVEYSFIKLVKKC
EECCEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]