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The map label for this gene is mdh [H]

Identifier: 157960678

GI number: 157960678

Start: 1049981

End: 1050916

Strand: Direct

Name: mdh [H]

Synonym: Spea_0850

Alternate gene names: 157960678

Gene position: 1049981-1050916 (Clockwise)

Preceding gene: 157960675

Following gene: 157960680

Centisome position: 20.29

GC content: 46.79

Gene sequence:

>936_bases
ATGAAAGTTGCTGTACTTGGTGCTGCTGGTGGTATTGGCCAGGCGCTTGCCCTATTGTTAAAAACTCAATTACCTGCGGG
TTCAAAATTGTCGCTTTATGATATCGCTCCAGTGACACCTGGTGTAGCGGTTGATCTAAGCCACATCCCTACAGCGGTTG
AAGTTAAAGGTTTCGCAGGTGAAGATCCGACTGCAGCGCTAGAAGGTGCTGATGTTGTTCTTATCTCTGCTGGCGTTGCA
CGTAAACCTGGAATGGATCGCTCAGATCTATTTAACATCAATGCTGGTATCGTTCGTAACTTAGTTGAAAAGTGCGCCGC
GACTTGCCCTAAAGCATTAATCGGTATTATCACTAACCCTGTCAATACAACTGTAGCGATTGCAGCCGAAGTATTGAAGA
ATGCAGGTGTTTACGACAAGAACCGCTTATTTGGGGTGACAACCCTTGATGTTATCCGCTCTGAAACTTTTGTTGCTGAA
GCAAAAGGCTTGAATGTTGCTGATGTTAAGGTGCCAGTTATTGGCGGCCATAGTGGTGTGACTATTCTGCCGCTGCTTTC
ACAAGTTGAAGGCGTAAGCTTTACTGATGCCGAAGTTGCAGCACTTACTACCCGTATTCAGAACGCGGGTACTGAGGTTG
TTGAGGCTAAAGCCGGTGGGGGTAGTGCGACACTTTCTATGGGGCAAGCGGCATGCCGTTTCGGTCTGTCATTAGTTCGC
GGCCTTCAAGGTGAAGCTAATGTTGTTGAGTGCGCTTACGTCGACGGTGGCAGTGAACATGCTGAATTCTTCGCACAACC
TGTTGTATTAGGTAAAAATGGCGTCGAAGAAGTGTTAGCTTACGGTGAAGTCAGTGCATTTGAAGCAAATGCACGTGATG
CGATGCTAGATACACTTCAGGCTGATATCCAACTAGGTATTGATTTCGTCAAGTAA

Upstream 100 bases:

>100_bases
CGACTAAGGGATAATTGAATTCTTTTTGTGAATCAAGTACTGTTGCGGCATTGGGAAGATGGATCTAAGTCACAAAATCC
GAGAATTAACGGAGATAATT

Downstream 100 bases:

>100_bases
TCAATTAGCTGCAATTAAAAAGGGAGCCACTAGGCTCCCTTTTTTGGTTTCTGATTTAGCCTTGTTGACTAATTGCTACG
TTGAGTCGCAATTTTAGCTA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFAGEDPTAALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNPVNTTVAIAAEVLKNAGVYDKNRLFGVTTLDVIRSETFVAE
AKGLNVADVKVPVIGGHSGVTILPLLSQVEGVSFTDAEVAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANVVECAYVDGGSEHAEFFAQPVVLGKNGVEEVLAYGEVSAFEANARDAMLDTLQADIQLGIDFVK

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFAGEDPTAALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNPVNTTVAIAAEVLKNAGVYDKNRLFGVTTLDVIRSETFVAE
AKGLNVADVKVPVIGGHSGVTILPLLSQVEGVSFTDAEVAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANVVECAYVDGGSEHAEFFAQPVVLGKNGVEEVLAYGEVSAFEANARDAMLDTLQADIQLGIDFVK
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFAGEDPTAALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNPVNTTVAIAAEVLKNAGVYDKNRLFGVTTLDVIRSETFVAE
AKGLNVADVKVPVIGGHSGVTILPLLSQVEGVSFTDAEVAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANVVECAYVDGGSEHAEFFAQPVVLGKNGVEEVLAYGEVSAFEANARDAMLDTLQADIQLGIDFVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=56.4102564102564, Blast_Score=326, Evalue=2e-89,
Organism=Escherichia coli, GI1789632, Length=310, Percent_Identity=81.2903225806452, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=58.14696485623, Blast_Score=331, Evalue=4e-91,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=49.8412698412698, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6324446, Length=369, Percent_Identity=37.6693766937669, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320125, Length=338, Percent_Identity=37.5739644970414, Blast_Score=182, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=56.7741935483871, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=49.8402555910543, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=44, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI17136226, Length=326, Percent_Identity=24.2331288343558, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 31824; Mature: 31824

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEHHHCCCEEEEECCCC
EDPTAALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTTVAIAAEVLKNAGVYDKNRLFGVTTLDVIRSETFVAEAKGLNVADVKVPVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
TILPLLSQVEGVSFTDAEVAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
CHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANVVECAYVDGGSEHAEFFAQPVVLGKNGVEEVLAYGEVSAFEANARDAMLDTLQ
CCCCCCCEEEEEEECCCCHHHHHHHCCEEECCCCHHHHHHHCCCCEECCCCHHHHHHHHH
ADIQLGIDFVK
HHHHHCCEECC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEHHHCCCEEEEECCCC
EDPTAALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTTVAIAAEVLKNAGVYDKNRLFGVTTLDVIRSETFVAEAKGLNVADVKVPVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
TILPLLSQVEGVSFTDAEVAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
CHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANVVECAYVDGGSEHAEFFAQPVVLGKNGVEEVLAYGEVSAFEANARDAMLDTLQ
CCCCCCCEEEEEEECCCCHHHHHHHCCEEECCCCHHHHHHHCCCCEECCCCHHHHHHHHH
ADIQLGIDFVK
HHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA