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The map label for this gene is yaaJ [C]

Identifier: 157960543

GI number: 157960543

Start: 879989

End: 881359

Strand: Direct

Name: yaaJ [C]

Synonym: Spea_0715

Alternate gene names: 157960543

Gene position: 879989-881359 (Clockwise)

Preceding gene: 157960542

Following gene: 157960544

Centisome position: 17.01

GC content: 48.5

Gene sequence:

>1371_bases
ATGAATTTTCACGCACTTTTAGCCGATATCAACGCCATTGTCTGGGGGCCTATTACCCTGTGCTTATTGGTGGGAACCGG
TCTTTATCTCACTGTTCGACTCAAACTGATCCAAGTTTTTAAATTGCCTATGGCGCTGGGTTTATTGTTTAAACCTGCAA
GTGGTAAGGGCGACTTATCATCATTTGCGGCGCTTTGTACCGCACTGTCGGCAACTATTGGTACCGGTAATATTGTGGGT
GTGGCAACCGCAATTAAAATCGGTGGTCCAGGGGCACTATTTTGGATGTGGCTAGCGGCCTTTTTTGGTATGGCGACCAA
GTACGCCGAATGTATGCTGGCAGTAAAGTATCGCACCACAGATGCCAAAGGTAATATTGCTGGTGGCCCTATGTACTATA
TCGAACGTGGCCTAGGGCTTAAGTGGCTGGCGAAGATCTTTGCCATCTTCGGTGTCGGCGTGGCGTTTTTTGGTATAGGT
ACCTTTGCTCAGGTTAATGCCATCAGTGATGCCATGACTATCGCTTTCGATATTCCTACTTGGGTAACTGCTGTTGCGCT
AACCTTGATGGTGGCGGCGGTGACACTGGGCGGCGTTAAGCGCATCGCTAATGTGGCGCAAAAGCTGGTGCCGACTATGG
CGCTGGGTTACGTGTTAGCCTGTATGTGGGTGCTGTTTATCTTCTCTGATCAAATTATTCCTGCATTTGAGTTGGTACTG
CACTCAGCTTTTACCCCTATCTCGGCTGCAGGTGGCTTCTTAGGCGCAACGGTTGCTCAAGCGCTGCAAATTGGTATCGC
TCGCGGCGTATTCTCTAATGAATCAGGCCTTGGTAGTGCACCTATTGCCGCCGCTGCCGCGAAGACTAACGAGCCGGTAG
AGCAAGGCCTTGTGAGCATGACAGGAACCTTCTTCGATACGATCTTAATTTGTACCATGACAGGTTTAGTACTGATTATT
ACCGGTGTGTGGAGCGGTGATACTGCAGGGGCTGCCATGACCAGCGCCGCATTTGCTGCCAGTGGCTCAGAAGTTATTGG
TCAATATGTGGTAACCATTGCACTGGTTTGTTTTGCCTTTACCACGATTTTAGGTTGGCATTATTACGGTGAGCGCTGCT
GGTATTACCTAACGGGCAGTCGCCTTGGTGATAACGGCATTAAAATCTACCAATTTACCTTTTTAGGCTTAATTGCAGGT
GGCGCATTTATTCACTTAGATCTGATTTGGATGCTAGCCGATACCGTAAACGGCTTGATGGCGATCCCTAACTTGATAGC
CCTCATAGGCTTAAGGCACGTGATCTTAAAAGAGACTCGCAAATATTTTGCTAAGCAGCAAGCCTTAGCGAACCAAAGCT
CGCTGGCTTAA

Upstream 100 bases:

>100_bases
GTTGCATACGTCAGTGCTCTGCAACAGCCATTTCTTTTCCTCCTCCGATTATTAATAACTCTTATTAGAGAGTTTTGTTG
TTTAATACTCGAGGAATACC

Downstream 100 bases:

>100_bases
GCTCTCATGCATGCAAGCGTGGAGCGTAAATAGTTTGCTTTAATTTAACATCGGCTAGATGAAAAATAAGCCCGCCCATC
ATTATTGAGGCGGGCTTTTT

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNFHALLADINAIVWGPITLCLLVGTGLYLTVRLKLIQVFKLPMALGLLFKPASGKGDLSSFAALCTALSATIGTGNIVG
VATAIKIGGPGALFWMWLAAFFGMATKYAECMLAVKYRTTDAKGNIAGGPMYYIERGLGLKWLAKIFAIFGVGVAFFGIG
TFAQVNAISDAMTIAFDIPTWVTAVALTLMVAAVTLGGVKRIANVAQKLVPTMALGYVLACMWVLFIFSDQIIPAFELVL
HSAFTPISAAGGFLGATVAQALQIGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSMTGTFFDTILICTMTGLVLII
TGVWSGDTAGAAMTSAAFAASGSEVIGQYVVTIALVCFAFTTILGWHYYGERCWYYLTGSRLGDNGIKIYQFTFLGLIAG
GAFIHLDLIWMLADTVNGLMAIPNLIALIGLRHVILKETRKYFAKQQALANQSSLA

Sequences:

>Translated_456_residues
MNFHALLADINAIVWGPITLCLLVGTGLYLTVRLKLIQVFKLPMALGLLFKPASGKGDLSSFAALCTALSATIGTGNIVG
VATAIKIGGPGALFWMWLAAFFGMATKYAECMLAVKYRTTDAKGNIAGGPMYYIERGLGLKWLAKIFAIFGVGVAFFGIG
TFAQVNAISDAMTIAFDIPTWVTAVALTLMVAAVTLGGVKRIANVAQKLVPTMALGYVLACMWVLFIFSDQIIPAFELVL
HSAFTPISAAGGFLGATVAQALQIGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSMTGTFFDTILICTMTGLVLII
TGVWSGDTAGAAMTSAAFAASGSEVIGQYVVTIALVCFAFTTILGWHYYGERCWYYLTGSRLGDNGIKIYQFTFLGLIAG
GAFIHLDLIWMLADTVNGLMAIPNLIALIGLRHVILKETRKYFAKQQALANQSSLA
>Mature_456_residues
MNFHALLADINAIVWGPITLCLLVGTGLYLTVRLKLIQVFKLPMALGLLFKPASGKGDLSSFAALCTALSATIGTGNIVG
VATAIKIGGPGALFWMWLAAFFGMATKYAECMLAVKYRTTDAKGNIAGGPMYYIERGLGLKWLAKIFAIFGVGVAFFGIG
TFAQVNAISDAMTIAFDIPTWVTAVALTLMVAAVTLGGVKRIANVAQKLVPTMALGYVLACMWVLFIFSDQIIPAFELVL
HSAFTPISAAGGFLGATVAQALQIGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSMTGTFFDTILICTMTGLVLII
TGVWSGDTAGAAMTSAAFAASGSEVIGQYVVTIALVCFAFTTILGWHYYGERCWYYLTGSRLGDNGIKIYQFTFLGLIAG
GAFIHLDLIWMLADTVNGLMAIPNLIALIGLRHVILKETRKYFAKQQALANQSSLA

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=450, Percent_Identity=35.3333333333333, Blast_Score=244, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 48130; Mature: 48130

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFHALLADINAIVWGPITLCLLVGTGLYLTVRLKLIQVFKLPMALGLLFKPASGKGDLS
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
SFAALCTALSATIGTGNIVGVATAIKIGGPGALFWMWLAAFFGMATKYAECMLAVKYRTT
HHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DAKGNIAGGPMYYIERGLGLKWLAKIFAIFGVGVAFFGIGTFAQVNAISDAMTIAFDIPT
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
WVTAVALTLMVAAVTLGGVKRIANVAQKLVPTMALGYVLACMWVLFIFSDQIIPAFELVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HSAFTPISAAGGFLGATVAQALQIGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSM
HHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
TGTFFDTILICTMTGLVLIITGVWSGDTAGAAMTSAAFAASGSEVIGQYVVTIALVCFAF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TTILGWHYYGERCWYYLTGSRLGDNGIKIYQFTFLGLIAGGAFIHLDLIWMLADTVNGLM
HHHHHHHHHCCEEEEEEECCEECCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AIPNLIALIGLRHVILKETRKYFAKQQALANQSSLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFHALLADINAIVWGPITLCLLVGTGLYLTVRLKLIQVFKLPMALGLLFKPASGKGDLS
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
SFAALCTALSATIGTGNIVGVATAIKIGGPGALFWMWLAAFFGMATKYAECMLAVKYRTT
HHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DAKGNIAGGPMYYIERGLGLKWLAKIFAIFGVGVAFFGIGTFAQVNAISDAMTIAFDIPT
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
WVTAVALTLMVAAVTLGGVKRIANVAQKLVPTMALGYVLACMWVLFIFSDQIIPAFELVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HSAFTPISAAGGFLGATVAQALQIGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSM
HHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
TGTFFDTILICTMTGLVLIITGVWSGDTAGAAMTSAAFAASGSEVIGQYVVTIALVCFAF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TTILGWHYYGERCWYYLTGSRLGDNGIKIYQFTFLGLIAGGAFIHLDLIWMLADTVNGLM
HHHHHHHHHCCEEEEEEECCEECCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AIPNLIALIGLRHVILKETRKYFAKQQALANQSSLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]