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The map label for this gene is mrcB [H]

Identifier: 157960533

GI number: 157960533

Start: 866859

End: 869231

Strand: Direct

Name: mrcB [H]

Synonym: Spea_0705

Alternate gene names: 157960533

Gene position: 866859-869231 (Clockwise)

Preceding gene: 157960532

Following gene: 157960534

Centisome position: 16.75

GC content: 50.91

Gene sequence:

>2373_bases
ATGACAGATAAAGCTACACCTAAAGCGGCCACTAAAAGAGCGCCTGCGAAGAAAAGAGCCACCACCAGAAATCCGAGAGC
GAAAAAGCCAGCGCCAAAGAAAGCCCCGAAGAAAGCGCCTTCGGGGTGGGGTAAGAAGATCTGGTCTATTAGCTGGAAGC
TTGGCTTAGTTTTAGCTGTGGCAGTCGCCTCTTATGGTATCTATCTCGACCAAATCATTGCCCGTAAGTTTGAAGGGCAG
AAGTGGCATCTTCCGGCGCAGGTGTTTAGCCGCTCTATGGCGCTATATCCAGGAGCTGCGGTTAGTCATGCTCAGTTAAT
GTCTGAACTTAAGCTACTTGGCTATCGTAAGGTGGCCAATCCGCGTCAGGTGGGGGAGTTTTCCGCCTCTAAGACCAAGA
TTGATCTTTGGCGTCGACCATTTTTGCATCCTCAAGGAGATCAGGTTGAACAGAGAGTGATGATCACCTTCGACAGTAAT
GGTGTGGTCTCAGTTGCCCGTAACAGCGATAAGCGTCAACTAGCCGTGTTCCACCTCGAACCTGTGCTTCTGGACAGGAT
CATCACAGGTGACGGCGAAGACAGGCTATTTGTGCCGACCGATAAGATCCCTAAGCCTATCGTTAATGCGCTTATCTTAG
TTGAAGACCGCAGTTTCTATGAGCATCATGGTGTTAATCCGTTTGCGATTCTGCGTGCGGCCATGGTGAATATCAGTGCC
GGGCGAACGGTGCAAGGCGGCTCGACCTTGACCCAACAGTTAGCGAAGAACTTCTTCCTGTCTAGTGAGCGCTCGCTTAG
CCGTAAATTGCGTGAAGCGCTGATGGCGATCATTATCGATTTTCGTTATGAAAAAGATGAGATCTTAGAAGCCTACCTCA
ATGAAGTGTACATGGGGCAAGACAAAGCCCGTGGCGTGCACGGTATGGGCTTAGCTTCACAGTTTTATTTTGGTCGACCA
ATCGCAGAGCTTACCGTACCGCAGCAGGCATTTTTGGTGGCGGTGATCAAAGGGCCTTCCTACTACAATCCATGGCGTTA
CCCTGAGCGCGCTCAAGAGCGACGCGATTTAGTATTGCGCTTGCTGATGGAGGCGGGCGATCTGAATGTGGCGCAGTATC
AAGCTGCAGTCGAGTCGCCACTGGGACTAAGAAAAGCCAACAAGTCAGTGCACCAAAAGTTACCGGCCTTCTTCTCAGTA
GTGAAGAATGAACTTGCCAGTCGCTATGGCGATGCCTTGTTGAAGCAATCAGGGGTTAAGGTCTACACCACGTTGGACCC
TATGGCTCAAGAAGCCGCCGAGAAAGCTGTTGAAAAGACCTTGAAGCAGCTGGGTAAAAATGATGAGAGCTTGCAGGTCG
GCATGGTACTAACCGATAAATACTCAGCTGGTATTGCCGCGATGGTAGGCGATAAAGTGCCTAGCTATCAGGGCTTTAAC
CGTGCCGTAGAAATTCGCAGACCCATAGGTTCGTTAATCAAACCTTTTGTCTATGCTACCGCGCTTAACCAAGGCGATAA
GTACAATTTGGTGTCGCCGTTAAAAGATCAGCCAATTACCCTTAAAAATGGTCAAGGTAAGACCTGGTCGCCGCAGAACG
TGGATAAGAAGTTCCGTGGTGAGGTGCCGCTGTTAACCGCATTTAAAAAGTCGATGAACGTGCCAACGGTAAACCTAGGC
ATGGCGATTGGAGTGAGTAGCGTGGCGACCACGCTGGACAAGGCGGGTTGGCGCGAGAAGATCCCTGAGTACCCATCTAT
GTTGCTCGGTGCGGTCAATGGCTCTCCCTTGATGGTGGCGCAGGTGTTCCAAACGATTGCCGATGATGGTCGTTATCGCC
ACTTGAACTCGGTGACTACGGTACTGGATGCGAATAATCAGCCACTAACGGCATCACGGCCTCAAGCGAGTCAAGCCATC
CCTACCGCCAGTAATTACCTGGTTAAATATGCTATGACTCAAGTGGTTGAATCTGGTACGGCTAAACGCTTAGGTAGCTC
ATACCCATATGTCACCTTGGCGGGTAAAACGGGTACCAGTAATGACTCACGAGACTCTTGGTTTGCCGGCTTTGACGAAC
GAAATGTGGCGGCCATCTGGATTGGCCGTGATGATAACGGCAAGACCGGGCTCTACGGCAGTAATGGCGCCATGGCCGTC
TATCAAGCCTTCTTAAGTAACCGTGCACCTCTTAGTCTTCGCATGGCGCCAGTCGATGGTGTAGTACAAGGTTACTTTGA
GCGCGATACTGGCAAAGCGCAGGAGCCACAATGTGCCAATGTGGTAAAGGTGCCAGCCCTGAGAGAAAGTTATCAGCCAG
TGGCTAATTGCGGCGAGCCACTGCCTTGGTGGAAGAAGCTGATTGGTGGTTAA

Upstream 100 bases:

>100_bases
GCATTTATGGCCAGATGATCCAGCCAACACTCAACCGACCAAATTCACTAAAAAGAAGACGTTTAGCTCGCAATAAGCTT
GAGCACGTCGCGGAAGTATA

Downstream 100 bases:

>100_bases
GCCCTTTATTCCCGAGACTTTCGAGCCACCAGGGTGTTTTGAGTCAAGCGACTTTCACTTTAGAGTGCTAGAAGAGCAAG
TCGCTGAGAAAGATTTTGAA

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 790; Mature: 789

Protein sequence:

>790_residues
MTDKATPKAATKRAPAKKRATTRNPRAKKPAPKKAPKKAPSGWGKKIWSISWKLGLVLAVAVASYGIYLDQIIARKFEGQ
KWHLPAQVFSRSMALYPGAAVSHAQLMSELKLLGYRKVANPRQVGEFSASKTKIDLWRRPFLHPQGDQVEQRVMITFDSN
GVVSVARNSDKRQLAVFHLEPVLLDRIITGDGEDRLFVPTDKIPKPIVNALILVEDRSFYEHHGVNPFAILRAAMVNISA
GRTVQGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQDKARGVHGMGLASQFYFGRP
IAELTVPQQAFLVAVIKGPSYYNPWRYPERAQERRDLVLRLLMEAGDLNVAQYQAAVESPLGLRKANKSVHQKLPAFFSV
VKNELASRYGDALLKQSGVKVYTTLDPMAQEAAEKAVEKTLKQLGKNDESLQVGMVLTDKYSAGIAAMVGDKVPSYQGFN
RAVEIRRPIGSLIKPFVYATALNQGDKYNLVSPLKDQPITLKNGQGKTWSPQNVDKKFRGEVPLLTAFKKSMNVPTVNLG
MAIGVSSVATTLDKAGWREKIPEYPSMLLGAVNGSPLMVAQVFQTIADDGRYRHLNSVTTVLDANNQPLTASRPQASQAI
PTASNYLVKYAMTQVVESGTAKRLGSSYPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWIGRDDNGKTGLYGSNGAMAV
YQAFLSNRAPLSLRMAPVDGVVQGYFERDTGKAQEPQCANVVKVPALRESYQPVANCGEPLPWWKKLIGG

Sequences:

>Translated_790_residues
MTDKATPKAATKRAPAKKRATTRNPRAKKPAPKKAPKKAPSGWGKKIWSISWKLGLVLAVAVASYGIYLDQIIARKFEGQ
KWHLPAQVFSRSMALYPGAAVSHAQLMSELKLLGYRKVANPRQVGEFSASKTKIDLWRRPFLHPQGDQVEQRVMITFDSN
GVVSVARNSDKRQLAVFHLEPVLLDRIITGDGEDRLFVPTDKIPKPIVNALILVEDRSFYEHHGVNPFAILRAAMVNISA
GRTVQGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQDKARGVHGMGLASQFYFGRP
IAELTVPQQAFLVAVIKGPSYYNPWRYPERAQERRDLVLRLLMEAGDLNVAQYQAAVESPLGLRKANKSVHQKLPAFFSV
VKNELASRYGDALLKQSGVKVYTTLDPMAQEAAEKAVEKTLKQLGKNDESLQVGMVLTDKYSAGIAAMVGDKVPSYQGFN
RAVEIRRPIGSLIKPFVYATALNQGDKYNLVSPLKDQPITLKNGQGKTWSPQNVDKKFRGEVPLLTAFKKSMNVPTVNLG
MAIGVSSVATTLDKAGWREKIPEYPSMLLGAVNGSPLMVAQVFQTIADDGRYRHLNSVTTVLDANNQPLTASRPQASQAI
PTASNYLVKYAMTQVVESGTAKRLGSSYPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWIGRDDNGKTGLYGSNGAMAV
YQAFLSNRAPLSLRMAPVDGVVQGYFERDTGKAQEPQCANVVKVPALRESYQPVANCGEPLPWWKKLIGG
>Mature_789_residues
TDKATPKAATKRAPAKKRATTRNPRAKKPAPKKAPKKAPSGWGKKIWSISWKLGLVLAVAVASYGIYLDQIIARKFEGQK
WHLPAQVFSRSMALYPGAAVSHAQLMSELKLLGYRKVANPRQVGEFSASKTKIDLWRRPFLHPQGDQVEQRVMITFDSNG
VVSVARNSDKRQLAVFHLEPVLLDRIITGDGEDRLFVPTDKIPKPIVNALILVEDRSFYEHHGVNPFAILRAAMVNISAG
RTVQGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQDKARGVHGMGLASQFYFGRPI
AELTVPQQAFLVAVIKGPSYYNPWRYPERAQERRDLVLRLLMEAGDLNVAQYQAAVESPLGLRKANKSVHQKLPAFFSVV
KNELASRYGDALLKQSGVKVYTTLDPMAQEAAEKAVEKTLKQLGKNDESLQVGMVLTDKYSAGIAAMVGDKVPSYQGFNR
AVEIRRPIGSLIKPFVYATALNQGDKYNLVSPLKDQPITLKNGQGKTWSPQNVDKKFRGEVPLLTAFKKSMNVPTVNLGM
AIGVSSVATTLDKAGWREKIPEYPSMLLGAVNGSPLMVAQVFQTIADDGRYRHLNSVTTVLDANNQPLTASRPQASQAIP
TASNYLVKYAMTQVVESGTAKRLGSSYPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWIGRDDNGKTGLYGSNGAMAVY
QAFLSNRAPLSLRMAPVDGVVQGYFERDTGKAQEPQCANVVKVPALRESYQPVANCGEPLPWWKKLIGG

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=694, Percent_Identity=44.5244956772334, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI87082258, Length=264, Percent_Identity=37.8787878787879, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1788867, Length=529, Percent_Identity=29.3005671077505, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=33.5294117647059, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 87110; Mature: 86979

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKATPKAATKRAPAKKRATTRNPRAKKPAPKKAPKKAPSGWGKKIWSISWKLGLVLAV
CCCCCCCCHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHH
AVASYGIYLDQIIARKFEGQKWHLPAQVFSRSMALYPGAAVSHAQLMSELKLLGYRKVAN
HHHHHHHHHHHHHHHHHCCCCEECCHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
PRQVGEFSASKTKIDLWRRPFLHPQGDQVEQRVMITFDSNGVVSVARNSDKRQLAVFHLE
CHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEEECC
PVLLDRIITGDGEDRLFVPTDKIPKPIVNALILVEDRSFYEHHGVNPFAILRAAMVNISA
HHHHHHHHCCCCCCEEEECCCCCCHHHHCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCC
GRTVQGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQ
CCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
DKARGVHGMGLASQFYFGRPIAELTVPQQAFLVAVIKGPSYYNPWRYPERAQERRDLVLR
HHHCCCCCCCCHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LLMEAGDLNVAQYQAAVESPLGLRKANKSVHQKLPAFFSVVKNELASRYGDALLKQSGVK
HHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VYTTLDPMAQEAAEKAVEKTLKQLGKNDESLQVGMVLTDKYSAGIAAMVGDKVPSYQGFN
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCH
RAVEIRRPIGSLIKPFVYATALNQGDKYNLVSPLKDQPITLKNGQGKTWSPQNVDKKFRG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCC
EVPLLTAFKKSMNVPTVNLGMAIGVSSVATTLDKAGWREKIPEYPSMLLGAVNGSPLMVA
CCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCCHHHH
QVFQTIADDGRYRHLNSVTTVLDANNQPLTASRPQASQAIPTASNYLVKYAMTQVVESGT
HHHHHHHCCCCEEEHHHHHHEEECCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCC
AKRLGSSYPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWIGRDDNGKTGLYGSNGAMAV
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEECCCCHHHH
YQAFLSNRAPLSLRMAPVDGVVQGYFERDTGKAQEPQCANVVKVPALRESYQPVANCGEP
HHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHCCHHHHCCCC
LPWWKKLIGG
CHHHHHHHCC
>Mature Secondary Structure 
TDKATPKAATKRAPAKKRATTRNPRAKKPAPKKAPKKAPSGWGKKIWSISWKLGLVLAV
CCCCCCCHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHH
AVASYGIYLDQIIARKFEGQKWHLPAQVFSRSMALYPGAAVSHAQLMSELKLLGYRKVAN
HHHHHHHHHHHHHHHHHCCCCEECCHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
PRQVGEFSASKTKIDLWRRPFLHPQGDQVEQRVMITFDSNGVVSVARNSDKRQLAVFHLE
CHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEEECC
PVLLDRIITGDGEDRLFVPTDKIPKPIVNALILVEDRSFYEHHGVNPFAILRAAMVNISA
HHHHHHHHCCCCCCEEEECCCCCCHHHHCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCC
GRTVQGGSTLTQQLAKNFFLSSERSLSRKLREALMAIIIDFRYEKDEILEAYLNEVYMGQ
CCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
DKARGVHGMGLASQFYFGRPIAELTVPQQAFLVAVIKGPSYYNPWRYPERAQERRDLVLR
HHHCCCCCCCCHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LLMEAGDLNVAQYQAAVESPLGLRKANKSVHQKLPAFFSVVKNELASRYGDALLKQSGVK
HHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VYTTLDPMAQEAAEKAVEKTLKQLGKNDESLQVGMVLTDKYSAGIAAMVGDKVPSYQGFN
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCH
RAVEIRRPIGSLIKPFVYATALNQGDKYNLVSPLKDQPITLKNGQGKTWSPQNVDKKFRG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCC
EVPLLTAFKKSMNVPTVNLGMAIGVSSVATTLDKAGWREKIPEYPSMLLGAVNGSPLMVA
CCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCCHHHH
QVFQTIADDGRYRHLNSVTTVLDANNQPLTASRPQASQAIPTASNYLVKYAMTQVVESGT
HHHHHHHCCCCEEEHHHHHHEEECCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCC
AKRLGSSYPYVTLAGKTGTSNDSRDSWFAGFDERNVAAIWIGRDDNGKTGLYGSNGAMAV
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEECCCCHHHH
YQAFLSNRAPLSLRMAPVDGVVQGYFERDTGKAQEPQCANVVKVPALRESYQPVANCGEP
HHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHCCHHHHCCCC
LPWWKKLIGG
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]