Click here to switch to the map view.

The map label for this gene is cysN [H]

Identifier: 157960492

GI number: 157960492

Start: 822927

End: 824366

Strand: Direct

Name: cysN [H]

Synonym: Spea_0664

Alternate gene names: 157960492

Gene position: 822927-824366 (Clockwise)

Preceding gene: 157960491

Following gene: 157960493

Centisome position: 15.9

GC content: 48.82

Gene sequence:

>1440_bases
ATGAATCAAGCAGTAAATAATAACGCGCGCCTGGCGGCAGAACTTGAAGATTTAGGTGTGAAAGAATACCTATCACAACA
GCAGCATAAAGGGCTATTACGCTTCTTAACTTGTGGCAGTGTCGATGACGGCAAGAGTACCCTAATCGGCCGTTTGCTAC
ATGACAGTGCACAAATTTATGAAGACCAACTGGCCAGTCTAAAGAGTGACAGTGCCAAGTTGGGTACAACAGGTGAAGAG
GTCGACTTAGCCCTGCTGGTGGATGGTCTGCAAGCGGAGCGCGAGCAGGGCATCACTATCGATGTAGCGTATCGTTATTT
CTCCAGTGACAAGCGTAAGTTCATTATTGCCGACACCCCGGGTCACGAGCAATACACTCGCAACATGGCTACAGGTGCTT
CGACTTGTGACTTAGCCGTACTCTTGGTCGATGCGCGTTATGGGGTACAAACTCAAACACGTCGTCACGCTTTTATCGCA
TCCCAGCTAGGTATTCGTCACTTTGTTGTGGCAGTAAACAAGATGGATCTGCTTGGTTTCGATGAGAAAGTCTTTAACGA
TATTCGCGCTGAGTTTGCCCAGTTTGCAGAGCAGTTAGGTGATATCGAAATTCGCTACGTACCCCTGTCAGCCTTAAAAG
GTGACAACGTCGTCGATGCCAGCTGTCAAACTCCTTGGTATAGCGGCGGCACCTTACTAGAGCTACTAGAAACGATTGAT
ACCCGTCGTGAACTTAACAGCCTACCAGTGCGTTTTCCGGTGCAATATGTACAGCGTCCAGATCTGGATTTTCGTGGCTT
TTCCGGCACGCTGGCATCGGGCGTAATTAACGTCGGTGACGAGTTGGTGGCACTGCCATCGGGCAAGCGCAGCAAGGTTG
CCCGTATTGTGACCTTCGATGGTGATCTGCCGCAGGCGATTGCGGGCCAAGCGGTGACCTTAACCCTTGAAGATGAGATT
GATATTTCAAGAGGCGATCTGTTGTCGCAGGTAGCTGACGCTCCAGCATTGGCTAACCACGTAAGCGCAGATATCGCTTG
GATGGATGAAAAACCACTACAGCTAGGTCAGTTGTATGATCTAAAAGTGGCAGGTAAAAAGCTGCAAGCGAGCGTAGTCA
AAATTGAATATGTGGCCGATATTAATACCTTAGAGCATAACGACAGCACTCATATTGGCCTTAATGATATGGCGCGAGTT
ACGCTCGAACTGAATGAGTCAATTGCTATCGACCCTTACACCTTGGTGCGTGATACTGGCGGCATGATCTTAATCGATCG
CCTGTCTAACGCGACGGTTGCGGCTGTCATGGCAGTATCGAGCACCAAAGAGCAGAAAACCGCTCAGCAATACAGTGAAT
TTGAGCTAGAGCTTAATGCGTTAATTCGTAAGCAGTATCCTCACTGGAATGCGATCGACATCAGTAAGGTCGAGGGTTAA

Upstream 100 bases:

>100_bases
TCAGATCAGAGTGCGTCGATGGAACTGAAAAAACGTCAGGGGTATTTCTAAGCCCATTACATCACAGATGAATGTGGATT
AAGAATTCTAAGGAATCATT

Downstream 100 bases:

>100_bases
CAGTGCCTGAATTGTGGGTGTTATCGTGCATATTGTTTGCCTTAGTCATCGCCTTAATGGCGGGGCTATGGACACCTGCT
GCGCTGTTTTTTATCGCATC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNQAVNNNARLAAELEDLGVKEYLSQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKLGTTGEE
VDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIA
SQLGIRHFVVAVNKMDLLGFDEKVFNDIRAEFAQFAEQLGDIEIRYVPLSALKGDNVVDASCQTPWYSGGTLLELLETID
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVARIVTFDGDLPQAIAGQAVTLTLEDEI
DISRGDLLSQVADAPALANHVSADIAWMDEKPLQLGQLYDLKVAGKKLQASVVKIEYVADINTLEHNDSTHIGLNDMARV
TLELNESIAIDPYTLVRDTGGMILIDRLSNATVAAVMAVSSTKEQKTAQQYSEFELELNALIRKQYPHWNAIDISKVEG

Sequences:

>Translated_479_residues
MNQAVNNNARLAAELEDLGVKEYLSQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKLGTTGEE
VDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIA
SQLGIRHFVVAVNKMDLLGFDEKVFNDIRAEFAQFAEQLGDIEIRYVPLSALKGDNVVDASCQTPWYSGGTLLELLETID
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVARIVTFDGDLPQAIAGQAVTLTLEDEI
DISRGDLLSQVADAPALANHVSADIAWMDEKPLQLGQLYDLKVAGKKLQASVVKIEYVADINTLEHNDSTHIGLNDMARV
TLELNESIAIDPYTLVRDTGGMILIDRLSNATVAAVMAVSSTKEQKTAQQYSEFELELNALIRKQYPHWNAIDISKVEG
>Mature_479_residues
MNQAVNNNARLAAELEDLGVKEYLSQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKLGTTGEE
VDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIA
SQLGIRHFVVAVNKMDLLGFDEKVFNDIRAEFAQFAEQLGDIEIRYVPLSALKGDNVVDASCQTPWYSGGTLLELLETID
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVARIVTFDGDLPQAIAGQAVTLTLEDEI
DISRGDLLSQVADAPALANHVSADIAWMDEKPLQLGQLYDLKVAGKKLQASVVKIEYVADINTLEHNDSTHIGLNDMARV
TLELNESIAIDPYTLVRDTGGMILIDRLSNATVAAVMAVSSTKEQKTAQQYSEFELELNALIRKQYPHWNAIDISKVEG

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=314, Percent_Identity=32.1656050955414, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI5729864, Length=314, Percent_Identity=32.1656050955414, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI4503475, Length=358, Percent_Identity=29.0502793296089, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI4503471, Length=335, Percent_Identity=29.2537313432836, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI194018520, Length=307, Percent_Identity=29.3159609120521, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI194097354, Length=307, Percent_Identity=29.3159609120521, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI194018522, Length=307, Percent_Identity=29.3159609120521, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI46094014, Length=311, Percent_Identity=28.6173633440514, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI34147630, Length=323, Percent_Identity=31.2693498452012, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI157426893, Length=274, Percent_Identity=27.007299270073, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789108, Length=459, Percent_Identity=61.437908496732, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1790412, Length=309, Percent_Identity=27.1844660194175, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1789737, Length=309, Percent_Identity=27.1844660194175, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI2367247, Length=262, Percent_Identity=29.0076335877863, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=353, Percent_Identity=30.8781869688385, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=353, Percent_Identity=30.8781869688385, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=438, Percent_Identity=26.4840182648402, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=296, Percent_Identity=30.4054054054054, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=296, Percent_Identity=30.4054054054054, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI32566303, Length=322, Percent_Identity=29.1925465838509, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.9565217391304, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=206, Percent_Identity=31.5533980582524, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=351, Percent_Identity=26.2108262108262, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325337, Length=331, Percent_Identity=30.8157099697885, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=331, Percent_Identity=30.8157099697885, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=409, Percent_Identity=29.8288508557457, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320377, Length=309, Percent_Identity=30.42071197411, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324761, Length=283, Percent_Identity=29.3286219081272, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=192, Percent_Identity=30.7291666666667, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550900, Length=456, Percent_Identity=27.1929824561404, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24652838, Length=309, Percent_Identity=29.126213592233, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI17137572, Length=309, Percent_Identity=29.126213592233, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI45553807, Length=270, Percent_Identity=31.1111111111111, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45553816, Length=270, Percent_Identity=31.1111111111111, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24651721, Length=270, Percent_Identity=31.1111111111111, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17864154, Length=270, Percent_Identity=31.1111111111111, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137380, Length=361, Percent_Identity=26.3157894736842, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281363316, Length=312, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17864358, Length=312, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19921738, Length=321, Percent_Identity=28.6604361370716, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52687; Mature: 52687

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAVNNNARLAAELEDLGVKEYLSQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEEHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKSDSAKLGTTGEEVDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIASQLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEECCHHCCCC
DEKVFNDIRAEFAQFAEQLGDIEIRYVPLSALKGDNVVDASCQTPWYSGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHH
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVARIVTFD
HHHHHHCCCCEECHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
GDLPQAIAGQAVTLTLEDEIDISRGDLLSQVADAPALANHVSADIAWMDEKPLQLGQLYD
CCCCHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHCCCEEECCCCCCCCCCEEE
LKVAGKKLQASVVKIEYVADINTLEHNDSTHIGLNDMARVTLELNESIAIDPYTLVRDTG
EEECCCHHEEEEEEEEEEECCCCCCCCCCCEECCCCCEEEEEEECCCEEECCEEEEECCC
GMILIDRLSNATVAAVMAVSSTKEQKTAQQYSEFELELNALIRKQYPHWNAIDISKVEG
CEEEEEECCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC
>Mature Secondary Structure
MNQAVNNNARLAAELEDLGVKEYLSQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEEHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKSDSAKLGTTGEEVDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIASQLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEECCHHCCCC
DEKVFNDIRAEFAQFAEQLGDIEIRYVPLSALKGDNVVDASCQTPWYSGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHH
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVARIVTFD
HHHHHHCCCCEECHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
GDLPQAIAGQAVTLTLEDEIDISRGDLLSQVADAPALANHVSADIAWMDEKPLQLGQLYD
CCCCHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHCCCEEECCCCCCCCCCEEE
LKVAGKKLQASVVKIEYVADINTLEHNDSTHIGLNDMARVTLELNESIAIDPYTLVRDTG
EEECCCHHEEEEEEEEEEECCCCCCCCCCCEECCCCCEEEEEEECCCEEECCEEEEECCC
GMILIDRLSNATVAAVMAVSSTKEQKTAQQYSEFELELNALIRKQYPHWNAIDISKVEG
CEEEEEECCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]