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The map label for this gene is purR [H]

Identifier: 157960348

GI number: 157960348

Start: 633517

End: 634521

Strand: Direct

Name: purR [H]

Synonym: Spea_0519

Alternate gene names: 157960348

Gene position: 633517-634521 (Clockwise)

Preceding gene: 157960347

Following gene: 157960351

Centisome position: 12.24

GC content: 50.85

Gene sequence:

>1005_bases
ATGGCCACCATGAAAGATGTCGCCAAACTTGCCAGCGTATCCACATCGACAGTGAGTCATGTGATGAATGGTACTCGCTT
TGTAAGTGATGATATTGCTAAGCGCGTGCAAGATGCCGCGCAGCAGTTAAATTATCATGGCCCTTCAGCGGTGGCTCGTA
GCCTTAAACAGAATTGCACTAAAACCATCGGCATGTTGGTAACCACCTCGACCAACCCATTCTATGCTGAGGTGGTGCGC
AGTGTTGAGCAAGCCTGTTATCAGCAAGGCTATAACCTTATTTTGTGTAATACCGAGGGTGATGCCCAACGCTTAAAGCG
CTCATTAACTACCTTGATTGAGCGACGCGTCGACGGTTTAATTATGATGTGCGCCATGTTAGAAGGTGACGATGCGCTGA
TCTATCGCCGCTTAGGTGGCTTACCTGTCGTGGTGATGGATTGGGGCCAAGTTGACTTTCCTTGCGACAAGATTCAGGGC
CACTCGCGCCAAGGTGGCATACTGGCGACGCAATACCTGATTGATAACGGCCATACGCAAATCGGCTGTATTACCGGCCC
GCGTAATCGCCAACAGGCTGACTTGCGTTACCAAGGTTTTGTCGATGCCATGGCGGCGGCTAATTTACCGATAAATCCGG
ATTGGGTCATTGAAGGTAACTTTGAAACCGAAGGCGGCAAGGCGGCGTTTGCTAAGCTGCAAGTCGCGGGCAAATTACCG
AGTGCGCTATTTGTGTGTAACGACATGATGGCTATTGGCGTACTGCATGAAGCACAGCGCAGCGGCGTGCAGATCCCTGA
GCAGTTATCGGTAATTGGTTACGACAACATTCAGCTGGCTAAGTTTATGACTCCGGCGCTAACCAGTATTCATCAGTCTA
AAGCGCGCTTAGGCGCAGCAGCGGTCAATGCCTTGATTGCACGCCTGCATGATCCAAGCCTGCCTCCGCAGTTTATGGAT
ATTGAGCCACGAGTGACCGAACGCGCCAGTGTGAAAAAAAGCTAA

Upstream 100 bases:

>100_bases
TTTCAAATAAAGAGTCATCAATTAAATCGGCATGAAATCGTCATTTATAACGAAAGTAATAGCGAAAGTAATAGCGTAAT
TCAGATAGGAAGTGAACCTC

Downstream 100 bases:

>100_bases
AAGGGCGAATAAAAAAGGCGAATGAATCAGCTTGGTAATAGTGAGCCACTCGCCTTTAATAACTGATTTATAAAATCAGT
TTATACAGAAAGCTTACTTA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MATMKDVAKLASVSTSTVSHVMNGTRFVSDDIAKRVQDAAQQLNYHGPSAVARSLKQNCTKTIGMLVTTSTNPFYAEVVR
SVEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGLIMMCAMLEGDDALIYRRLGGLPVVVMDWGQVDFPCDKIQG
HSRQGGILATQYLIDNGHTQIGCITGPRNRQQADLRYQGFVDAMAAANLPINPDWVIEGNFETEGGKAAFAKLQVAGKLP
SALFVCNDMMAIGVLHEAQRSGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAAAVNALIARLHDPSLPPQFMD
IEPRVTERASVKKS

Sequences:

>Translated_334_residues
MATMKDVAKLASVSTSTVSHVMNGTRFVSDDIAKRVQDAAQQLNYHGPSAVARSLKQNCTKTIGMLVTTSTNPFYAEVVR
SVEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGLIMMCAMLEGDDALIYRRLGGLPVVVMDWGQVDFPCDKIQG
HSRQGGILATQYLIDNGHTQIGCITGPRNRQQADLRYQGFVDAMAAANLPINPDWVIEGNFETEGGKAAFAKLQVAGKLP
SALFVCNDMMAIGVLHEAQRSGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAAAVNALIARLHDPSLPPQFMD
IEPRVTERASVKKS
>Mature_333_residues
ATMKDVAKLASVSTSTVSHVMNGTRFVSDDIAKRVQDAAQQLNYHGPSAVARSLKQNCTKTIGMLVTTSTNPFYAEVVRS
VEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGLIMMCAMLEGDDALIYRRLGGLPVVVMDWGQVDFPCDKIQGH
SRQGGILATQYLIDNGHTQIGCITGPRNRQQADLRYQGFVDAMAAANLPINPDWVIEGNFETEGGKAAFAKLQVAGKLPS
ALFVCNDMMAIGVLHEAQRSGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAAAVNALIARLHDPSLPPQFMDI
EPRVTERASVKKS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=51.2121212121212, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=42.3423423423423, Blast_Score=279, Evalue=1e-76,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=32.6923076923077, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=32.9896907216495, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=28.0597014925373, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=29.1095890410959, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787580, Length=334, Percent_Identity=27.2455089820359, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786540, Length=307, Percent_Identity=28.6644951140065, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI48994940, Length=326, Percent_Identity=24.8466257668712, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787906, Length=268, Percent_Identity=27.6119402985075, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786268, Length=337, Percent_Identity=22.8486646884273, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789456, Length=341, Percent_Identity=23.4604105571848, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=21.474358974359, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790689, Length=336, Percent_Identity=25.5952380952381, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36422; Mature: 36291

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVAKLASVSTSTVSHVMNGTRFVSDDIAKRVQDAAQQLNYHGPSAVARSLKQNCT
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KTIGMLVTTSTNPFYAEVVRSVEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGL
HHHHEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
IMMCAMLEGDDALIYRRLGGLPVVVMDWGQVDFPCDKIQGHSRQGGILATQYLIDNGHTQ
HHHHHHHCCCCHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCCCCCEEEEEEEEECCCEE
IGCITGPRNRQQADLRYQGFVDAMAAANLPINPDWVIEGNFETEGGKAAFAKLQVAGKLP
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHEEECCCCC
SALFVCNDMMAIGVLHEAQRSGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVNALIARLHDPSLPPQFMDIEPRVTERASVKKS
HHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
ATMKDVAKLASVSTSTVSHVMNGTRFVSDDIAKRVQDAAQQLNYHGPSAVARSLKQNCT
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KTIGMLVTTSTNPFYAEVVRSVEQACYQQGYNLILCNTEGDAQRLKRSLTTLIERRVDGL
HHHHEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
IMMCAMLEGDDALIYRRLGGLPVVVMDWGQVDFPCDKIQGHSRQGGILATQYLIDNGHTQ
HHHHHHHCCCCHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCCCCCEEEEEEEEECCCEE
IGCITGPRNRQQADLRYQGFVDAMAAANLPINPDWVIEGNFETEGGKAAFAKLQVAGKLP
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHEEECCCCC
SALFVCNDMMAIGVLHEAQRSGVQIPEQLSVIGYDNIQLAKFMTPALTSIHQSKARLGAA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVNALIARLHDPSLPPQFMDIEPRVTERASVKKS
HHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]