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The map label for this gene is purH [H]

Identifier: 157960261

GI number: 157960261

Start: 537651

End: 539240

Strand: Reverse

Name: purH [H]

Synonym: Spea_0432

Alternate gene names: 157960261

Gene position: 539240-537651 (Counterclockwise)

Preceding gene: 157960276

Following gene: 157960260

Centisome position: 10.42

GC content: 48.36

Gene sequence:

>1590_bases
ATGAATAATGCCAGACCTATTCGTCGCGCGCTGTTAAGCGTTTCAGATAAAACCGGAATCCTAGAGTTTGCACAAGCGCT
GCATGCTCAAGGTGTTGAGCTACTTTCTACTGGTGGCACCGCACGCCTGCTGGCTGATAATGGCGTGCCTGTCATTGAAG
TATCAGATTATACTGGTCACCCAGAGATCATGGATGGCCGCGTTAAAACCCTGCACCCTAAAGTGCATGGCGGTATTTTA
GCGCGTCGCGGTATCGACGAAATCGTAATGGAACAAAACAACATCAAGCCTATCGACTTGGTTGCAGTTAACTTGTATCC
GTTTGCTGCTACCGTTGCTCAAGAAGGTTGCACGCTTGCTGACGCTATCGAGAACATCGATATCGGCGGCCCTACTATGG
TGCGATCTACCGCTAAAAACCATAAAGACACTACTATCATCGTTAACGCGAAAGATTACGGCCGCGTTATTGCAGAAATG
CAATCAAACGAAGCTAGCACTACCCTTGAGACTCGCTTTGATCTAGCGATTGCAGCGTTTGAGCACACTGCCGCATACGA
TGGCATGATTGCTAACTACTTCGGCACTAAAGTACCTGCACACAGCAATGACGAGTGCCATGAAGATTCTAAGTTCCCAC
GCACTTACAACACTCAGCTAGTTAAGAAGCAAGACTTGCGCTATGGCGAAAATAGCCACCAAACAGCCGCTTTCTATGTT
GATACAAATCTTGATGAAGCCTCTGTTGCCACCGCCGTTCAACTGCAAGGTAAGGCACTGTCATACAATAACATCGCCGA
TACCGACTCTGCACTTGAGTGCGTAAAAGAGTTCGACGAGCCAGCTTGTGTGATTGTTAAGCATGCTAATCCATGTGGTG
TGGCTATTGGTGAAAACCTACTTGAAGCTTATAACCGTGCATTCCAAACTGACCCAACGTCGGCTTTCGGTGGCATCATC
GCCTTTAACGGCGAGCTAGATGCAGCAACTGCTAGCGCGATTGTTGAGCGCCAGTTTGTTGAGGTGATCATAGCCCCTAA
AGTTAGCCAAGCAGCCCGTGACGTTATTGCTGCTAAAGCAAACGTACGCTTGCTTGAGTGTGGTGAGTGGGCAGCAAAAA
CCACCAGCCTAGATTACAAGCGTGTAAACGGCGGCCTATTACTACAAGATCGCGACCAAGGCATGGTTGGTCTTGATGAC
GTTAAGGTTGTTTCTAAGCGCCAACCAACGGCTGAAGAGATGAAAGATCTAATGTTCTGCTGGAAAGTGGCTAAGTTTGT
TAAATCAAACGCCATTGTTTACGCCAAGAACAGCATGACAATCGGTGTAGGTGCAGGCCAAATGAGCCGAGTTTACAGCG
CTAAGGTTGCAGGTATTAAAGCCGCAGACGAAGGCCTAGAAGTACAAAACTCAGTAATGGCATCAGATGCGTTCTTCCCA
TTCCGTGATGGTATCGATGCTGCAGCAGAAGCAGGCATTAGCTGTATCATCCAGCCTGGCGGCTCTATCCGCGATGAAGA
GATCATCGCAGCAGCTGATGAGCACGGCATGGCAATGGTATTTACCGGTATGCGTCACTTCCGTCATTAA

Upstream 100 bases:

>100_bases
GAGGGACTTTTGGAAAGCAGTGTAGGCCGCTATAGAGCGAGCAATACTGTTCTGTTTTCCGAAAGATCCTTAAAAATAAC
TACTGGACCCTGTACCTACA

Downstream 100 bases:

>100_bases
TTGGTTTGCGCGAATTAAGCAATAAAGGACTTTATTCGCGCATTAACAAAATTGATATTTAACGTTTAGAGCAAGCTATT
CTTTCTAGGTCAAAGAAAGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHGGIL
ARRGIDEIVMEQNNIKPIDLVAVNLYPFAATVAQEGCTLADAIENIDIGGPTMVRSTAKNHKDTTIIVNAKDYGRVIAEM
QSNEASTTLETRFDLAIAAFEHTAAYDGMIANYFGTKVPAHSNDECHEDSKFPRTYNTQLVKKQDLRYGENSHQTAAFYV
DTNLDEASVATAVQLQGKALSYNNIADTDSALECVKEFDEPACVIVKHANPCGVAIGENLLEAYNRAFQTDPTSAFGGII
AFNGELDAATASAIVERQFVEVIIAPKVSQAARDVIAAKANVRLLECGEWAAKTTSLDYKRVNGGLLLQDRDQGMVGLDD
VKVVSKRQPTAEEMKDLMFCWKVAKFVKSNAIVYAKNSMTIGVGAGQMSRVYSAKVAGIKAADEGLEVQNSVMASDAFFP
FRDGIDAAAEAGISCIIQPGGSIRDEEIIAAADEHGMAMVFTGMRHFRH

Sequences:

>Translated_529_residues
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHGGIL
ARRGIDEIVMEQNNIKPIDLVAVNLYPFAATVAQEGCTLADAIENIDIGGPTMVRSTAKNHKDTTIIVNAKDYGRVIAEM
QSNEASTTLETRFDLAIAAFEHTAAYDGMIANYFGTKVPAHSNDECHEDSKFPRTYNTQLVKKQDLRYGENSHQTAAFYV
DTNLDEASVATAVQLQGKALSYNNIADTDSALECVKEFDEPACVIVKHANPCGVAIGENLLEAYNRAFQTDPTSAFGGII
AFNGELDAATASAIVERQFVEVIIAPKVSQAARDVIAAKANVRLLECGEWAAKTTSLDYKRVNGGLLLQDRDQGMVGLDD
VKVVSKRQPTAEEMKDLMFCWKVAKFVKSNAIVYAKNSMTIGVGAGQMSRVYSAKVAGIKAADEGLEVQNSVMASDAFFP
FRDGIDAAAEAGISCIIQPGGSIRDEEIIAAADEHGMAMVFTGMRHFRH
>Mature_529_residues
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHGGIL
ARRGIDEIVMEQNNIKPIDLVAVNLYPFAATVAQEGCTLADAIENIDIGGPTMVRSTAKNHKDTTIIVNAKDYGRVIAEM
QSNEASTTLETRFDLAIAAFEHTAAYDGMIANYFGTKVPAHSNDECHEDSKFPRTYNTQLVKKQDLRYGENSHQTAAFYV
DTNLDEASVATAVQLQGKALSYNNIADTDSALECVKEFDEPACVIVKHANPCGVAIGENLLEAYNRAFQTDPTSAFGGII
AFNGELDAATASAIVERQFVEVIIAPKVSQAARDVIAAKANVRLLECGEWAAKTTSLDYKRVNGGLLLQDRDQGMVGLDD
VKVVSKRQPTAEEMKDLMFCWKVAKFVKSNAIVYAKNSMTIGVGAGQMSRVYSAKVAGIKAADEGLEVQNSVMASDAFFP
FRDGIDAAAEAGISCIIQPGGSIRDEEIIAAADEHGMAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=518, Percent_Identity=37.0656370656371, Blast_Score=273, Evalue=3e-73,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=73.7240075614367, Blast_Score=835, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=514, Percent_Identity=33.0739299610895, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71985574, Length=344, Percent_Identity=26.453488372093, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=36.1538461538462, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323768, Length=492, Percent_Identity=35.7723577235772, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6323056, Length=500, Percent_Identity=34.2, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24649832, Length=612, Percent_Identity=33.9869281045752, Blast_Score=294, Evalue=9e-80,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57106; Mature: 57106

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGH
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCCCCC
PEIMDGRVKTLHPKVHGGILARRGIDEIVMEQNNIKPIDLVAVNLYPFAATVAQEGCTLA
CCCCCCCEEEECCHHHCCHHHHCCHHHHHHCCCCCCEEEEEEEEEEHHHHHHHHCCCCHH
DAIENIDIGGPTMVRSTAKNHKDTTIIVNAKDYGRVIAEMQSNEASTTLETRFDLAIAAF
HHHHCCCCCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHEEEEEEH
EHTAAYDGMIANYFGTKVPAHSNDECHEDSKFPRTYNTQLVKKQDLRYGENSHQTAAFYV
HHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEE
DTNLDEASVATAVQLQGKALSYNNIADTDSALECVKEFDEPACVIVKHANPCGVAIGENL
ECCCCHHHHHEEEEECCCEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
LEAYNRAFQTDPTSAFGGIIAFNGELDAATASAIVERQFVEVIIAPKVSQAARDVIAAKA
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHC
NVRLLECGEWAAKTTSLDYKRVNGGLLLQDRDQGMVGLDDVKVVSKRQPTAEEMKDLMFC
CEEEEECCCCHHHCCCCCEEECCCCEEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKNSMTIGVGAGQMSRVYSAKVAGIKAADEGLEVQNSVMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCHHHHHHHHCCCCCC
FRDGIDAAAEAGISCIIQPGGSIRDEEIIAAADEHGMAMVFTGMRHFRH
CCCCCCHHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEHHHHHCC
>Mature Secondary Structure
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGH
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCCCCC
PEIMDGRVKTLHPKVHGGILARRGIDEIVMEQNNIKPIDLVAVNLYPFAATVAQEGCTLA
CCCCCCCEEEECCHHHCCHHHHCCHHHHHHCCCCCCEEEEEEEEEEHHHHHHHHCCCCHH
DAIENIDIGGPTMVRSTAKNHKDTTIIVNAKDYGRVIAEMQSNEASTTLETRFDLAIAAF
HHHHCCCCCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCCCCHHHHHEEEEEEH
EHTAAYDGMIANYFGTKVPAHSNDECHEDSKFPRTYNTQLVKKQDLRYGENSHQTAAFYV
HHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEE
DTNLDEASVATAVQLQGKALSYNNIADTDSALECVKEFDEPACVIVKHANPCGVAIGENL
ECCCCHHHHHEEEEECCCEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
LEAYNRAFQTDPTSAFGGIIAFNGELDAATASAIVERQFVEVIIAPKVSQAARDVIAAKA
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHC
NVRLLECGEWAAKTTSLDYKRVNGGLLLQDRDQGMVGLDDVKVVSKRQPTAEEMKDLMFC
CEEEEECCCCHHHCCCCCEEECCCCEEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKNSMTIGVGAGQMSRVYSAKVAGIKAADEGLEVQNSVMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCHHHHHHHHCCCCCC
FRDGIDAAAEAGISCIIQPGGSIRDEEIIAAADEHGMAMVFTGMRHFRH
CCCCCCHHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA