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The map label for this gene is purD [H]

Identifier: 157960260

GI number: 157960260

Start: 536172

End: 537473

Strand: Reverse

Name: purD [H]

Synonym: Spea_0431

Alternate gene names: 157960260

Gene position: 537473-536172 (Counterclockwise)

Preceding gene: 157960261

Following gene: 157960259

Centisome position: 10.39

GC content: 48.85

Gene sequence:

>1302_bases
ATGAAAGTATTGATTATAGGTGGCGGCGGTCGCGAACATGCATTGGCATGGAAGGCCGCGCAATCGGCGCAAGTTGAAAC
TGTATTTGTTGCCCCAGGTAACGCTGGTACAGCATTAGAGCCAAAGCTTGAAAACGTGGCGATTAACGTTGAAGAGATTT
CGGCGTTAGTGGCATTTGCCGAAGAGCAAAAAGTCGCTATCACCATTGTTGGCCCTGAAGTACCACTAGCATTGGGTGTA
GTTGACGCCTTTAATGAAGCCGGCCTGCCTATCTTTGGCCCAACTAAAGGTGCAGCGCAGCTAGAATCGTCTAAAGCCTT
TACTAAAGACTTCCTAGCACGTCATAACATTCCAACTGCGGCTTACTCAAACTTTACCGAGATAAAGCCAGCCAAAGCTT
ACGTTAAACAAGTGACTGGCCTGACTGGTTTCCCTATCGTGATCAAGGCCGATGGCCTTGCCGCTGGTAAAGGCGTTATC
ATCGCAGCAGACCTAACTGAAGCCAATGCAGCTATTGAAGATATGCTGGCTGGTAACAAGTTTGGTGAGGCGGGTTCTCG
CGTGGTTATCGAAGAGTTCCTTAAAGGTGAAGAAGCCAGCTTTATCGTAATGGTCGATGGTAAGAACATTCTTGCAATGG
CCACCAGCCAAGACCATAAAGCCCGTGATAATGGCGATCATGGTCCAAACACTGGCGGCATGGGCGCATACTCGCCAGCG
CCTGTTGTTACTCAAGCCGTTCATGACTGGACTATTGAGCATGTTATTCGCCCAACAGTTGATGGCATGGCTGCAGAAGG
CAATGTATACACAGGCTTCTTATATGCAGGCTTGATGATTTCACCGGATGGCAGCGCTAAAGTACTTGAGTACAACTGCC
GCTTTGGCGACCCAGAAACTCAGCCAATTATGATGCGTCTTAAATCTGATCTGGTTGAGCTTTGCCTAGCATCGACTCGT
GGTGAACTGGATCAAGTCACAGCTGAATTTGATTCTCGCGCAGCCGTAGGTGTGGTACTTGCCGCTGGCGGATACCCAGA
TGCATACCGCAAACACGATGTGATTGACGGTCTATCTCTTGGTAACAACGACGCTAAAGTATTCCATGCTGGCACTAGCA
TGCAAGATGGTCACGTTGTGACTAATGGCGGCCGCGTGTTATGTGCTACCGCACTGGGCAATACAGTGACTGAAGCGCAG
CAAGCGGCTTATAACCTAGTTGATGCAATTCACTGGGATGACGTGTACTTTAGAACAGACATTGCTTACCGTGCTATAGC
TCGTGAGAATAACGAAGACTAA

Upstream 100 bases:

>100_bases
ATTCTTTCTAGGTCAAAGAAAGCACGCGCAACGTATGCCAATACGTGAGCATGCTTGATGCAGAGATAGGAAGAAAGGCG
CAGCTATAAAAGGTAAAGAT

Downstream 100 bases:

>100_bases
TCCACTAAAAGCAATTGGGATGACGGCTGTTATAACTTAACCGAACCGATATCGCATACCGCATACCGCGCAATTGCTCG
CGAAAACGGCGAAGGCTAAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKVLIIGGGGREHALAWKAAQSAQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFAEEQKVAITIVGPEVPLALGV
VDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTAAYSNFTEIKPAKAYVKQVTGLTGFPIVIKADGLAAGKGVI
IAADLTEANAAIEDMLAGNKFGEAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLASTR
GELDQVTAEFDSRAAVGVVLAAGGYPDAYRKHDVIDGLSLGNNDAKVFHAGTSMQDGHVVTNGGRVLCATALGNTVTEAQ
QAAYNLVDAIHWDDVYFRTDIAYRAIARENNED

Sequences:

>Translated_433_residues
MKVLIIGGGGREHALAWKAAQSAQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFAEEQKVAITIVGPEVPLALGV
VDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTAAYSNFTEIKPAKAYVKQVTGLTGFPIVIKADGLAAGKGVI
IAADLTEANAAIEDMLAGNKFGEAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLASTR
GELDQVTAEFDSRAAVGVVLAAGGYPDAYRKHDVIDGLSLGNNDAKVFHAGTSMQDGHVVTNGGRVLCATALGNTVTEAQ
QAAYNLVDAIHWDDVYFRTDIAYRAIARENNED
>Mature_433_residues
MKVLIIGGGGREHALAWKAAQSAQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFAEEQKVAITIVGPEVPLALGV
VDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTAAYSNFTEIKPAKAYVKQVTGLTGFPIVIKADGLAAGKGVI
IAADLTEANAAIEDMLAGNKFGEAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLASTR
GELDQVTAEFDSRAAVGVVLAAGGYPDAYRKHDVIDGLSLGNNDAKVFHAGTSMQDGHVVTNGGRVLCATALGNTVTEAQ
QAAYNLVDAIHWDDVYFRTDIAYRAIARENNED

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=432, Percent_Identity=47.6851851851852, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=432, Percent_Identity=47.6851851851852, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=432, Percent_Identity=47.6851851851852, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI28416899, Length=432, Percent_Identity=47.6851851851852, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=433, Percent_Identity=69.7459584295612, Blast_Score=602, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=40.8983451536643, Blast_Score=295, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6321203, Length=449, Percent_Identity=42.3162583518931, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=43.778801843318, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=43.778801843318, Blast_Score=341, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45718; Mature: 45718

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLIIGGGGREHALAWKAAQSAQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFA
CEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHH
EEQKVAITIVGPEVPLALGVVDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHH
AYSNFTEIKPAKAYVKQVTGLTGFPIVIKADGLAAGKGVIIAADLTEANAAIEDMLAGNK
HHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCC
FGEAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPET
HHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCC
QPIMMRLKSDLVELCLASTRGELDQVTAEFDSRAAVGVVLAAGGYPDAYRKHDVIDGLSL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCCCCCCC
GNNDAKVFHAGTSMQDGHVVTNGGRVLCATALGNTVTEAQQAAYNLVDAIHWDDVYFRTD
CCCCCEEEECCCCCCCCEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHEECCCEEEEEC
IAYRAIARENNED
HHHHHHHHCCCCC
>Mature Secondary Structure
MKVLIIGGGGREHALAWKAAQSAQVETVFVAPGNAGTALEPKLENVAINVEEISALVAFA
CEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHH
EEQKVAITIVGPEVPLALGVVDAFNEAGLPIFGPTKGAAQLESSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHH
AYSNFTEIKPAKAYVKQVTGLTGFPIVIKADGLAAGKGVIIAADLTEANAAIEDMLAGNK
HHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCC
FGEAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNGDHGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQAVHDWTIEHVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPET
HHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCC
QPIMMRLKSDLVELCLASTRGELDQVTAEFDSRAAVGVVLAAGGYPDAYRKHDVIDGLSL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCCCCCCC
GNNDAKVFHAGTSMQDGHVVTNGGRVLCATALGNTVTEAQQAAYNLVDAIHWDDVYFRTD
CCCCCEEEECCCCCCCCEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHEECCCEEEEEC
IAYRAIARENNED
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]