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The map label for this gene is dmlR [H]

Identifier: 157960225

GI number: 157960225

Start: 478350

End: 479264

Strand: Direct

Name: dmlR [H]

Synonym: Spea_0396

Alternate gene names: 157960225

Gene position: 478350-479264 (Clockwise)

Preceding gene: 157960224

Following gene: 157960228

Centisome position: 9.24

GC content: 48.63

Gene sequence:

>915_bases
ATGCAGCTTAACGATCTGAAACTGTTTATCAGGGTTGCGGACTGCGGTAGTTTGACTGCGGCTGCGGTTGAATTAGACAT
TTCAACTGCGGTGGCGAGCGCAGGCCTGAAGCGGCTAGAAAAAGAGTTAGCTACGACCTTATTCATTCGCAGCACTCGCA
GCCTAAGGTTAACCGATGCCGGCGAGCGCTATCTTATCCACTGCCGTCGAGCCTTAGCCGACCTAGAGCTAGGCCATAGA
GCGATAGAAAGCAGTCAGGCGAGTATTTCAGGGCTGCTTAGCTTAGCGGTTCCTTCAGATATTGGCCGTAACGTTTTACT
CCCTTGGTTAGATGAGTTCCTCGTTGAATATTCAGAGCTTAAGCTGCGCCTACATATTGGCGATAATCTGTCGGATTTTT
ATCATGACAAGATAGATGTGGCATTGCGCTCAGGTGTTCCTAAAGACTCTTCGCTGGTGGCCTTTAAAATCTGTCATTCA
CGCCGGGTGCTTTGTGCTTCGCCTGACTATATTCGACAGTTTGGTGAACCGAGCGCTTTAGATCAATTACCGCAACATAA
TTGCTTATTCTTTATGCTGGATGAGCGCACTCATGATCAGTGGGTCTTCAATAAAGATGGTCTGCAACATAAGGTGAGAG
TGACGGCTAATCGTACCTGCAATGATGCCGATATAGCTCGGCGTTGGGCGGTTGCAGGTCAAGGGGTGGTATTTAAGTCT
TGGTTAGATCTGACCGACGATATCGAGTCTGGGCGATTAGTGCCTTTACTCAATGAATATCAAGGTGAAGAGGCCGACTT
ATATTTAGTTTGCCCAGGGCGGGAGCATGTGACGCCTGTGATCCTGTTGCTGCGAGATATGTTACGTGAGCGCTGTAAAC
AACGTTTAGCGCGGGTGAGTCACTTGATGGAGTGA

Upstream 100 bases:

>100_bases
AGAGCCTGCATTGCGCAGGCTTTTTTGTGGGTGACGTTTAGTCTCTGTTTATCTACAATTTGATTTAAATAAATGCATAT
TTATAAATGGAATATTGATA

Downstream 100 bases:

>100_bases
TCAAATAGCAGACACAAAAAAACCGCACGAGTGCGGTTTTTTTATCAATGACGCCTAAGTTAGCTCTTAGCGCGCTTCAT
CGCAGTGAAGAACTCATCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MQLNDLKLFIRVADCGSLTAAAVELDISTAVASAGLKRLEKELATTLFIRSTRSLRLTDAGERYLIHCRRALADLELGHR
AIESSQASISGLLSLAVPSDIGRNVLLPWLDEFLVEYSELKLRLHIGDNLSDFYHDKIDVALRSGVPKDSSLVAFKICHS
RRVLCASPDYIRQFGEPSALDQLPQHNCLFFMLDERTHDQWVFNKDGLQHKVRVTANRTCNDADIARRWAVAGQGVVFKS
WLDLTDDIESGRLVPLLNEYQGEEADLYLVCPGREHVTPVILLLRDMLRERCKQRLARVSHLME

Sequences:

>Translated_304_residues
MQLNDLKLFIRVADCGSLTAAAVELDISTAVASAGLKRLEKELATTLFIRSTRSLRLTDAGERYLIHCRRALADLELGHR
AIESSQASISGLLSLAVPSDIGRNVLLPWLDEFLVEYSELKLRLHIGDNLSDFYHDKIDVALRSGVPKDSSLVAFKICHS
RRVLCASPDYIRQFGEPSALDQLPQHNCLFFMLDERTHDQWVFNKDGLQHKVRVTANRTCNDADIARRWAVAGQGVVFKS
WLDLTDDIESGRLVPLLNEYQGEEADLYLVCPGREHVTPVILLLRDMLRERCKQRLARVSHLME
>Mature_304_residues
MQLNDLKLFIRVADCGSLTAAAVELDISTAVASAGLKRLEKELATTLFIRSTRSLRLTDAGERYLIHCRRALADLELGHR
AIESSQASISGLLSLAVPSDIGRNVLLPWLDEFLVEYSELKLRLHIGDNLSDFYHDKIDVALRSGVPKDSSLVAFKICHS
RRVLCASPDYIRQFGEPSALDQLPQHNCLFFMLDERTHDQWVFNKDGLQHKVRVTANRTCNDADIARRWAVAGQGVVFKS
WLDLTDDIESGRLVPLLNEYQGEEADLYLVCPGREHVTPVILLLRDMLRERCKQRLARVSHLME

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=301, Percent_Identity=31.5614617940199, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789440, Length=295, Percent_Identity=27.1186440677966, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786401, Length=253, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1789639, Length=268, Percent_Identity=30.2238805970149, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI145693193, Length=279, Percent_Identity=28.3154121863799, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=25, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787128, Length=279, Percent_Identity=24.7311827956989, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=28.6206896551724, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789173, Length=270, Percent_Identity=27.4074074074074, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=232, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790011, Length=325, Percent_Identity=27.3846153846154, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=131, Percent_Identity=26.7175572519084, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788297, Length=139, Percent_Identity=30.9352517985612, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790208, Length=172, Percent_Identity=27.3255813953488, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34362; Mature: 34362

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNDLKLFIRVADCGSLTAAAVELDISTAVASAGLKRLEKELATTLFIRSTRSLRLTDA
CCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
GERYLIHCRRALADLELGHRAIESSQASISGLLSLAVPSDIGRNVLLPWLDEFLVEYSEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHE
KLRLHIGDNLSDFYHDKIDVALRSGVPKDSSLVAFKICHSRRVLCASPDYIRQFGEPSAL
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCHHHHHCCCCHHH
DQLPQHNCLFFMLDERTHDQWVFNKDGLQHKVRVTANRTCNDADIARRWAVAGQGVVFKS
HHCCCCCEEEEEEECCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEHH
WLDLTDDIESGRLVPLLNEYQGEEADLYLVCPGREHVTPVILLLRDMLRERCKQRLARVS
HHCHHCCCCCCCEEEEHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HLME
HHCC
>Mature Secondary Structure
MQLNDLKLFIRVADCGSLTAAAVELDISTAVASAGLKRLEKELATTLFIRSTRSLRLTDA
CCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
GERYLIHCRRALADLELGHRAIESSQASISGLLSLAVPSDIGRNVLLPWLDEFLVEYSEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHE
KLRLHIGDNLSDFYHDKIDVALRSGVPKDSSLVAFKICHSRRVLCASPDYIRQFGEPSAL
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCHHHHHCCCCHHH
DQLPQHNCLFFMLDERTHDQWVFNKDGLQHKVRVTANRTCNDADIARRWAVAGQGVVFKS
HHCCCCCEEEEEEECCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEHH
WLDLTDDIESGRLVPLLNEYQGEEADLYLVCPGREHVTPVILLLRDMLRERCKQRLARVS
HHCHHCCCCCCCEEEEHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HLME
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]