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The map label for this gene is pucG [H]

Identifier: 157960179

GI number: 157960179

Start: 398269

End: 399405

Strand: Direct

Name: pucG [H]

Synonym: Spea_0350

Alternate gene names: 157960179

Gene position: 398269-399405 (Clockwise)

Preceding gene: 157960178

Following gene: 157960184

Centisome position: 7.7

GC content: 50.84

Gene sequence:

>1137_bases
ATGTTACCCGCACCTTTTATTGAGCCTTTTAGTCCCCCACGTAGAATTTTGATGGGTCCAGGCCCCTCAGATGTTTATCC
TGAGGTGCTGGCGGCGCAAGCCAAGCCAACAATTGGCCACCTTGACCCACTATTTGTCGAGATGATGGATGAGCTTAAAG
CGTTGATCCAATATGCCTTCCAGACCAACAACGAGATGACCATGGCGGTTTCGGCCCCTGGTAGTGCCGGTATGGAAACC
TGCTTTGTGAATCTGGTTGAACCGGGTGAGAAAGTCATTGTTTGCCGTAATGGCGTGTTCGGTGAGCGTATGCGCCAGAA
CGTTGAGCGTGTTGGCGGCGTTGCAGTCGTGGTGGATAACGAATGGGGTGAGGCGGTCGATACCGACCAAGTTGCTAGCG
CATTGGCGGCTAATCCTGATGCAAAATTTCTGGCATTTGTGCATGCTGAAACATCAACGGGTGCTCTATCAGATGCTAAA
GCCTTATGTGCACTAGCCAAGCAGCATGACTGCTTATCGATAGTCGATGCTGTGACCTCTTTAGGTGGCGTCGAGTTGCT
GGTGGATGAATGGGGTATTGATGCGATTTACTCAGGTAGCCAGAAGTGCTTATCTTGCGTGCCAGGCTTATCACCTGTTT
CCTTCTCTCCTGCCGCGGTTGCCAAGTTAAAGGCGCGTAAAACACCTGTGCAGAGTTGGTTCTTGGATCAAACTCTAGTC
ATGGGTTATTGGGATGCATCGAGTGATGGCAAGCGCAGCTACCATCATACCGCGCCAGTCAATGCCCTCTATGCACTGCA
TGAGTCACTGCGTTTATTGGCGGCTGAAGGGCTAGAGCTTGCTTGGCAGCGCCATGCCGATATGCACCAAGTGTTGCGAC
ATGGTTTGCAACGTCTAGGGCTTAAGTTTGTGGTAGCTGAAGCGTGTCGTTTACCTCAATTAAATGCGGTTTATATTCCT
GAGGGGATCGATGACGCTAAGATCAGAAAGCAGCTGCTAGAGAACTATAACCTTGAGATAGGTGCGGGTCTTGGTGCGCT
TTCGGGTAAGGCGTGGCGCATCGGCTTGATGGGCTTTGGTGCAAGGCGCGAAAATGTGGCTTTATGCCTTAAGGCATTAG
AAGAAGTATTGGCTTAG

Upstream 100 bases:

>100_bases
CTGACTAACACGTAGGGGCTTAATGATATTTTCGTTATAGCAGCATGGCGGCAGTCGAACTTTAACCTAATACCAAGCGT
CCAGTTTTAGAGGAAATATC

Downstream 100 bases:

>100_bases
TTTTAAGGGCTTTCTACTGAGCGCTATGCCATTACGTTAAGCCAAAGTCGGATTAATAAACAAAGACCCCAGCTTAAGCT
GGGGTCTTTGTTTTAAAAGC

Product: Serine--pyruvate transaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLPAPFIEPFSPPRRILMGPGPSDVYPEVLAAQAKPTIGHLDPLFVEMMDELKALIQYAFQTNNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGGVAVVVDNEWGEAVDTDQVASALAANPDAKFLAFVHAETSTGALSDAK
ALCALAKQHDCLSIVDAVTSLGGVELLVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPAAVAKLKARKTPVQSWFLDQTLV
MGYWDASSDGKRSYHHTAPVNALYALHESLRLLAAEGLELAWQRHADMHQVLRHGLQRLGLKFVVAEACRLPQLNAVYIP
EGIDDAKIRKQLLENYNLEIGAGLGALSGKAWRIGLMGFGARRENVALCLKALEEVLA

Sequences:

>Translated_378_residues
MLPAPFIEPFSPPRRILMGPGPSDVYPEVLAAQAKPTIGHLDPLFVEMMDELKALIQYAFQTNNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGGVAVVVDNEWGEAVDTDQVASALAANPDAKFLAFVHAETSTGALSDAK
ALCALAKQHDCLSIVDAVTSLGGVELLVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPAAVAKLKARKTPVQSWFLDQTLV
MGYWDASSDGKRSYHHTAPVNALYALHESLRLLAAEGLELAWQRHADMHQVLRHGLQRLGLKFVVAEACRLPQLNAVYIP
EGIDDAKIRKQLLENYNLEIGAGLGALSGKAWRIGLMGFGARRENVALCLKALEEVLA
>Mature_378_residues
MLPAPFIEPFSPPRRILMGPGPSDVYPEVLAAQAKPTIGHLDPLFVEMMDELKALIQYAFQTNNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGGVAVVVDNEWGEAVDTDQVASALAANPDAKFLAFVHAETSTGALSDAK
ALCALAKQHDCLSIVDAVTSLGGVELLVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPAAVAKLKARKTPVQSWFLDQTLV
MGYWDASSDGKRSYHHTAPVNALYALHESLRLLAAEGLELAWQRHADMHQVLRHGLQRLGLKFVVAEACRLPQLNAVYIP
EGIDDAKIRKQLLENYNLEIGAGLGALSGKAWRIGLMGFGARRENVALCLKALEEVLA

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=376, Percent_Identity=39.6276595744681, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17536281, Length=378, Percent_Identity=36.5079365079365, Blast_Score=253, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6321079, Length=372, Percent_Identity=27.6881720430108, Blast_Score=102, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17530823, Length=381, Percent_Identity=37.7952755905512, Blast_Score=262, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 40818; Mature: 40818

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPAPFIEPFSPPRRILMGPGPSDVYPEVLAAQAKPTIGHLDPLFVEMMDELKALIQYAF
CCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QTNNEMTMAVSAPGSAGMETCFVNLVEPGEKVIVCRNGVFGERMRQNVERVGGVAVVVDN
HCCCCEEEEEECCCCCCHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEC
EWGEAVDTDQVASALAANPDAKFLAFVHAETSTGALSDAKALCALAKQHDCLSIVDAVTS
CCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LGGVELLVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPAAVAKLKARKTPVQSWFLDQTLV
CCCEEEEEECCCCCHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCEEE
MGYWDASSDGKRSYHHTAPVNALYALHESLRLLAAEGLELAWQRHADMHQVLRHGLQRLG
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHH
LKFVVAEACRLPQLNAVYIPEGIDDAKIRKQLLENYNLEIGAGLGALSGKAWRIGLMGFG
HHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECC
ARRENVALCLKALEEVLA
CCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLPAPFIEPFSPPRRILMGPGPSDVYPEVLAAQAKPTIGHLDPLFVEMMDELKALIQYAF
CCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QTNNEMTMAVSAPGSAGMETCFVNLVEPGEKVIVCRNGVFGERMRQNVERVGGVAVVVDN
HCCCCEEEEEECCCCCCHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEC
EWGEAVDTDQVASALAANPDAKFLAFVHAETSTGALSDAKALCALAKQHDCLSIVDAVTS
CCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LGGVELLVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPAAVAKLKARKTPVQSWFLDQTLV
CCCEEEEEECCCCCHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCEEE
MGYWDASSDGKRSYHHTAPVNALYALHESLRLLAAEGLELAWQRHADMHQVLRHGLQRLG
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHH
LKFVVAEACRLPQLNAVYIPEGIDDAKIRKQLLENYNLEIGAGLGALSGKAWRIGLMGFG
HHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECC
ARRENVALCLKALEEVLA
CCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]