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The map label for this gene is ilvA [H]

Identifier: 157960178

GI number: 157960178

Start: 396382

End: 397932

Strand: Direct

Name: ilvA [H]

Synonym: Spea_0349

Alternate gene names: 157960178

Gene position: 396382-397932 (Clockwise)

Preceding gene: 157960177

Following gene: 157960179

Centisome position: 7.66

GC content: 49.97

Gene sequence:

>1551_bases
ATGTTGGCTCTAGCATCACAATCGCAACTGGATTTGGCTCACTATTATCTGCAGAAAACTTTGCTGTCATCGGTTTATGA
TGTGGCAAAAGTTACGCCGCTTTCCAGCCTTAATAAACTGTCTGCGCGCTTAGGCTGTCAGGTATTTTTAAAACGTGAAG
ATATGCAGCCTGTACACTCTTTCAAGCTGCGCGGTGCTTACAACAAGATAGCCGAATTGACTAAAGAGGAATGTGAATGT
GGCGTGGTGTGCGCCTCGGCGGGCAACCATGCTCAAGGCGTTGCTATGTCGGCGTCAGCTCGTGGTATTAGCGCGGTTAT
TGTGATGCCAGAGACCACTCCTGACATTAAGGTGGATGCGGTTAGGCGTTTAGGTGGTGAAGTGGTACTTCATGGCCAAG
CGTTCGATCAGGCTAATGCTTATGCCCAAACTCTTGCGAAGAATGAAGGTCGAGTCTATGTGGCGCCATTCGATGATGAA
GCTGTGATTGCAGGGCAAGGCACGGTGGCGCAAGAGATGTTGCAGCAGCAGCGCGATCTTGAGGTGGTCTTTGTGCCTGT
GGGCGGTGGCGGCTTGATTGCAGGGGTTGCGGCTTACTATAAAGCGGTCATGCCACAGGTGAAGATTATTGGTGTCGAGC
CGGAAGACTCAGCCTGTTTAAAGGCTGCACTGGATGCCGGTGAGCGAGTGATATTGCCGCAAGTTGGCTTGTTTGCCGAT
GGTGTGGCGGTTAAACAGATAGGCGCCGAGCCTTTTAGATTAGCTCGTGAGTACGTCGATGATGTGATCACCGTGACCTC
AGATGAAATCTGCGCCGCGGTGAAAGATATCTTCGAAGACACTCGCGCCATTGCCGAGCCGTCTGGAGCATTGTCGCTAG
CAGGGCTGAAGAAGTATATCGGCAGTAACGGTAACGGTGAGAAAGTCGGTGCTATTTTGAGTGGCGCTAACGTGAACTTT
CATAGCCTGCGCTATGTGTCTGAACGCTGTGAACTCGGTGAGCAGAAAGAAGCGATTTTGGCGGTGAAAGTACCAGAGCG
CCCTGGCGTGTTTCTGCGCTTTTGTGAGCTGCTAGACAAGCGAGCGATGACCGAATTTAACTACCGTTTCTGTAGCCGTG
ATAAGGCGGTGGTATTTGCGGGAATTCGTTTGACTCAGGGGCAAAGTGAGCTCGATGGGATTATCGCCAAGCTGGAGTCT
GAAGGTTTTGATGTGCAAGACCTGTCTAATGATGAAACTGCCAAATTGCATGTGCGTTACATGGTGGGTGGTCAGCCGCC
AGAGCCACTTGAGGAGCGTTTGTTTAGCTTTGAGTTCCCTGAGCACCCTGGCGCACTGCTTAAGTTTTTAACCACCTTGC
AGAGTAAGTGGAATATCAGCCTATTTCATTATCGTAATCATGGCGCAGCCTACGGCCGAGTATTGGCAGGATTTGAGGTG
CCAGAAAGCGATAATTTGGCGTTTAAACAGTTTTTAACTGAGTTAGGTTTTGTCTATCAAGAGGAAACTGATAGCCCGGC
TTATCAGCTTTTCTTGGATAACCGTAATTAG

Upstream 100 bases:

>100_bases
CTTAAAGCCTACGCATTATTAGCGACCAGTGCCGATAAGGGCGCGGTGCGTGATACTAGCAAGCTAGTTTAATCGATTTA
ACGGGATTAAGAGGTAGTTT

Downstream 100 bases:

>100_bases
GCGGTATAGCTAAGTTAGTCGTTTAAAGTGGGCAGCAGAGATTTTCGATGCTGCCTTTTTTTATCTGCGATCTTCTTGAG
TTTTACTAAAGTCGATACAA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MLALASQSQLDLAHYYLQKTLLSSVYDVAKVTPLSSLNKLSARLGCQVFLKREDMQPVHSFKLRGAYNKIAELTKEECEC
GVVCASAGNHAQGVAMSASARGISAVIVMPETTPDIKVDAVRRLGGEVVLHGQAFDQANAYAQTLAKNEGRVYVAPFDDE
AVIAGQGTVAQEMLQQQRDLEVVFVPVGGGGLIAGVAAYYKAVMPQVKIIGVEPEDSACLKAALDAGERVILPQVGLFAD
GVAVKQIGAEPFRLAREYVDDVITVTSDEICAAVKDIFEDTRAIAEPSGALSLAGLKKYIGSNGNGEKVGAILSGANVNF
HSLRYVSERCELGEQKEAILAVKVPERPGVFLRFCELLDKRAMTEFNYRFCSRDKAVVFAGIRLTQGQSELDGIIAKLES
EGFDVQDLSNDETAKLHVRYMVGGQPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAAYGRVLAGFEV
PESDNLAFKQFLTELGFVYQEETDSPAYQLFLDNRN

Sequences:

>Translated_516_residues
MLALASQSQLDLAHYYLQKTLLSSVYDVAKVTPLSSLNKLSARLGCQVFLKREDMQPVHSFKLRGAYNKIAELTKEECEC
GVVCASAGNHAQGVAMSASARGISAVIVMPETTPDIKVDAVRRLGGEVVLHGQAFDQANAYAQTLAKNEGRVYVAPFDDE
AVIAGQGTVAQEMLQQQRDLEVVFVPVGGGGLIAGVAAYYKAVMPQVKIIGVEPEDSACLKAALDAGERVILPQVGLFAD
GVAVKQIGAEPFRLAREYVDDVITVTSDEICAAVKDIFEDTRAIAEPSGALSLAGLKKYIGSNGNGEKVGAILSGANVNF
HSLRYVSERCELGEQKEAILAVKVPERPGVFLRFCELLDKRAMTEFNYRFCSRDKAVVFAGIRLTQGQSELDGIIAKLES
EGFDVQDLSNDETAKLHVRYMVGGQPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAAYGRVLAGFEV
PESDNLAFKQFLTELGFVYQEETDSPAYQLFLDNRN
>Mature_516_residues
MLALASQSQLDLAHYYLQKTLLSSVYDVAKVTPLSSLNKLSARLGCQVFLKREDMQPVHSFKLRGAYNKIAELTKEECEC
GVVCASAGNHAQGVAMSASARGISAVIVMPETTPDIKVDAVRRLGGEVVLHGQAFDQANAYAQTLAKNEGRVYVAPFDDE
AVIAGQGTVAQEMLQQQRDLEVVFVPVGGGGLIAGVAAYYKAVMPQVKIIGVEPEDSACLKAALDAGERVILPQVGLFAD
GVAVKQIGAEPFRLAREYVDDVITVTSDEICAAVKDIFEDTRAIAEPSGALSLAGLKKYIGSNGNGEKVGAILSGANVNF
HSLRYVSERCELGEQKEAILAVKVPERPGVFLRFCELLDKRAMTEFNYRFCSRDKAVVFAGIRLTQGQSELDGIIAKLES
EGFDVQDLSNDETAKLHVRYMVGGQPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAAYGRVLAGFEV
PESDNLAFKQFLTELGFVYQEETDSPAYQLFLDNRN

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=337, Percent_Identity=32.6409495548961, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI19923959, Length=317, Percent_Identity=33.1230283911672, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI33469958, Length=310, Percent_Identity=29.0322580645161, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI295821202, Length=199, Percent_Identity=30.1507537688442, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI295821200, Length=199, Percent_Identity=30.1507537688442, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4557415, Length=199, Percent_Identity=30.1507537688442, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790207, Length=498, Percent_Identity=62.0481927710843, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI1789505, Length=301, Percent_Identity=38.2059800664452, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789235, Length=250, Percent_Identity=27.6, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI2367138, Length=206, Percent_Identity=27.6699029126214, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71991565, Length=320, Percent_Identity=34.375, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17537387, Length=349, Percent_Identity=30.945558739255, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17508781, Length=278, Percent_Identity=30.5755395683453, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320930, Length=520, Percent_Identity=50.5769230769231, Blast_Score=480, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322631, Length=298, Percent_Identity=37.248322147651, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6319788, Length=286, Percent_Identity=25.5244755244755, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321594, Length=215, Percent_Identity=26.5116279069767, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355833, Length=314, Percent_Identity=33.1210191082803, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645328, Length=250, Percent_Identity=32, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24643623, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129101, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 56317; Mature: 56317

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALASQSQLDLAHYYLQKTLLSSVYDVAKVTPLSSLNKLSARLGCQVFLKREDMQPVHS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCHHHH
FKLRGAYNKIAELTKEECECGVVCASAGNHAQGVAMSASARGISAVIVMPETTPDIKVDA
HHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHH
VRRLGGEVVLHGQAFDQANAYAQTLAKNEGRVYVAPFDDEAVIAGQGTVAQEMLQQQRDL
HHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCHHHHHHHHHCCCC
EVVFVPVGGGGLIAGVAAYYKAVMPQVKIIGVEPEDSACLKAALDAGERVILPQVGLFAD
EEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCEEECCCCCCCCC
GVAVKQIGAEPFRLAREYVDDVITVTSDEICAAVKDIFEDTRAIAEPSGALSLAGLKKYI
CHHHHHHCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
GSNGNGEKVGAILSGANVNFHSLRYVSERCELGEQKEAILAVKVPERPGVFLRFCELLDK
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
RAMTEFNYRFCSRDKAVVFAGIRLTQGQSELDGIIAKLESEGFDVQDLSNDETAKLHVRY
HHHHHHCHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
MVGGQPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAAYGRVLAGFEV
EECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHEECCCC
PESDNLAFKQFLTELGFVYQEETDSPAYQLFLDNRN
CCCCCHHHHHHHHHHCCEEECCCCCCEEEEEEECCC
>Mature Secondary Structure
MLALASQSQLDLAHYYLQKTLLSSVYDVAKVTPLSSLNKLSARLGCQVFLKREDMQPVHS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCHHHH
FKLRGAYNKIAELTKEECECGVVCASAGNHAQGVAMSASARGISAVIVMPETTPDIKVDA
HHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHH
VRRLGGEVVLHGQAFDQANAYAQTLAKNEGRVYVAPFDDEAVIAGQGTVAQEMLQQQRDL
HHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCHHHHHHHHHCCCC
EVVFVPVGGGGLIAGVAAYYKAVMPQVKIIGVEPEDSACLKAALDAGERVILPQVGLFAD
EEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCEEECCCCCCCCC
GVAVKQIGAEPFRLAREYVDDVITVTSDEICAAVKDIFEDTRAIAEPSGALSLAGLKKYI
CHHHHHHCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
GSNGNGEKVGAILSGANVNFHSLRYVSERCELGEQKEAILAVKVPERPGVFLRFCELLDK
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
RAMTEFNYRFCSRDKAVVFAGIRLTQGQSELDGIIAKLESEGFDVQDLSNDETAKLHVRY
HHHHHHCHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
MVGGQPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAAYGRVLAGFEV
EECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHEECCCC
PESDNLAFKQFLTELGFVYQEETDSPAYQLFLDNRN
CCCCCHHHHHHHHHHCCEEECCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]