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The map label for this gene is nrfC [H]

Identifier: 157960089

GI number: 157960089

Start: 291532

End: 292218

Strand: Direct

Name: nrfC [H]

Synonym: Spea_0260

Alternate gene names: 157960089

Gene position: 291532-292218 (Clockwise)

Preceding gene: 157960088

Following gene: 157960090

Centisome position: 5.63

GC content: 47.16

Gene sequence:

>687_bases
ATGAGCATGAATAAGAATAGCTCTGAAATGGATACTGGCCGGCGTCACTTCCTTGCCGCTATGGGTGGTGTCGGTATGTT
GATAGGTTTTAGTGGTACAGCCTATGCGATACGCGACGATAAAGTTTATATCAAAAGCTCAGGTGGTGCATTGGTGGTCG
ATCTCAAGAAATGCATGGGCTGTGGTACCTGTATGACTACCTGCTCATTAGTACATCATGGCAAAGCTTCGCTGTCTTTG
TCATGCATTCAGATTGAGCAGGATGTATTTAAGCCGTTTCCACAAGATATTCAGATGAGTACCTGCCTGCAATGTCACGA
TGCCCCTTGCGTGACAGCCTGTCCAGTTAATGCCAATAAGGCGAGTGAAGACTTTGATTTTGTGCGCGATATCGATCCTG
ATAAGTGTATTGGCTGTATGCAGTGTATCGAAGCCTGCCCCCATACCCCATCACGAGTCCAGTGGAACCCGACAACCCGC
TGCGCACAGAAATGCGATTTGTGCCTCAACACTCCCTATATGGAAGAGCAAGGTGGGATTGGTGGTGTTCAGGCATGTAG
GAGAGTCTGTCCAGTTGGTGCAATTGCTTTTATTACTGAGCTACCAGAGCAAAGCCTGCCGCAAAATTATGTCGTGAATT
TGCGAAATGAAAACTGGGCTAAATCCGGTAAGACTATCGAAGACTGA

Upstream 100 bases:

>100_bases
CCATGGTAACTGGTTATGTTAGTCGTGATTATGCGCAAAAAGAACACGCTATATGGTTTAGAGAATTAGAAGAAAAGCAG
CGTCATCAGGAGTCGCAACG

Downstream 100 bases:

>100_bases
GAAATTTCATGAAAAAATTAAAAGGTTACGCAGGCAAAATCCTGCACATTGATTTAAATAATATGACTCATCATGTTTTG
CCTACTGAAGATTATTATGA

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSMNKNSSEMDTGRRHFLAAMGGVGMLIGFSGTAYAIRDDKVYIKSSGGALVVDLKKCMGCGTCMTTCSLVHHGKASLSL
SCIQIEQDVFKPFPQDIQMSTCLQCHDAPCVTACPVNANKASEDFDFVRDIDPDKCIGCMQCIEACPHTPSRVQWNPTTR
CAQKCDLCLNTPYMEEQGGIGGVQACRRVCPVGAIAFITELPEQSLPQNYVVNLRNENWAKSGKTIED

Sequences:

>Translated_228_residues
MSMNKNSSEMDTGRRHFLAAMGGVGMLIGFSGTAYAIRDDKVYIKSSGGALVVDLKKCMGCGTCMTTCSLVHHGKASLSL
SCIQIEQDVFKPFPQDIQMSTCLQCHDAPCVTACPVNANKASEDFDFVRDIDPDKCIGCMQCIEACPHTPSRVQWNPTTR
CAQKCDLCLNTPYMEEQGGIGGVQACRRVCPVGAIAFITELPEQSLPQNYVVNLRNENWAKSGKTIED
>Mature_227_residues
SMNKNSSEMDTGRRHFLAAMGGVGMLIGFSGTAYAIRDDKVYIKSSGGALVVDLKKCMGCGTCMTTCSLVHHGKASLSLS
CIQIEQDVFKPFPQDIQMSTCLQCHDAPCVTACPVNANKASEDFDFVRDIDPDKCIGCMQCIEACPHTPSRVQWNPTTRC
AQKCDLCLNTPYMEEQGGIGGVQACRRVCPVGAIAFITELPEQSLPQNYVVNLRNENWAKSGKTIED

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=191, Percent_Identity=35.0785340314136, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI226510944, Length=198, Percent_Identity=31.8181818181818, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789370, Length=214, Percent_Identity=27.5700934579439, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082179, Length=170, Percent_Identity=31.7647058823529, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082180, Length=173, Percent_Identity=31.7919075144509, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787749, Length=204, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790326, Length=193, Percent_Identity=26.4248704663212, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788811, Length=162, Percent_Identity=28.3950617283951, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=151, Percent_Identity=32.4503311258278, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1789067, Length=164, Percent_Identity=31.0975609756098, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24832; Mature: 24701

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
12.3 %Cys+Met (Translated Protein)
7.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
11.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNKNSSEMDTGRRHFLAAMGGVGMLIGFSGTAYAIRDDKVYIKSSGGALVVDLKKCMG
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCEEEEECCCCEEEEEHHHHCC
CGTCMTTCSLVHHGKASLSLSCIQIEQDVFKPFPQDIQMSTCLQCHDAPCVTACPVNANK
CCHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCCCCCC
ASEDFDFVRDIDPDKCIGCMQCIEACPHTPSRVQWNPTTRCAQKCDLCLNTPYMEEQGGI
CCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHCCHHCCCCCCCCCCCC
GGVQACRRVCPVGAIAFITELPEQSLPQNYVVNLRNENWAKSGKTIED
CHHHHHHHHCCHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SMNKNSSEMDTGRRHFLAAMGGVGMLIGFSGTAYAIRDDKVYIKSSGGALVVDLKKCMG
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCEEEEECCCCEEEEEHHHHCC
CGTCMTTCSLVHHGKASLSLSCIQIEQDVFKPFPQDIQMSTCLQCHDAPCVTACPVNANK
CCHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCCCCCC
ASEDFDFVRDIDPDKCIGCMQCIEACPHTPSRVQWNPTTRCAQKCDLCLNTPYMEEQGGI
CCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHCCHHCCCCCCCCCCCC
GGVQACRRVCPVGAIAFITELPEQSLPQNYVVNLRNENWAKSGKTIED
CHHHHHHHHCCHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]