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The map label for this gene is tuf1 [H]

Identifier: 157960012

GI number: 157960012

Start: 208877

End: 210061

Strand: Direct

Name: tuf1 [H]

Synonym: Spea_0182

Alternate gene names: 157960012

Gene position: 208877-210061 (Clockwise)

Preceding gene: 157960011

Following gene: 157960013

Centisome position: 4.04

GC content: 45.99

Gene sequence:

>1185_bases
GTGGCTAAAGAAAAATTTGAACGCGTAAAACCGCATGTTAACGTTGGTACTATTGGACACGTTGACCATGGTAAAACTAC
TCTAACTGCTGCTATCTCTTCGGTACTAACGAAGACTTACGGTGGTACTAAGCGTGATTTCGCACAGATCGATAACGCTC
CAGAAGAGCGTGAGCGCGGTATTACAATTAATACTTCTCACATCGAATATGACACTCCATCACGTCACTACGCACACGTA
GACTGTCCAGGTCACGCGGATTATGTTAAAAACATGATCACTGGTGCTGCACAGATGGACGGCGCTATTCTAGTAGTTGC
TTCTACAGATGGTCCAATGCCACAAACTCGTGAGCACATCCTGCTTTCACGTCAGGTTGGCGTACCATTCATCATCGTAT
TCATGAACAAGTGTGACATGGTTGATGACGAAGAGCTTCTAGAGCTAGTAGAAATGGAAGTTCGTGAACTTCTATCTGAG
TATGACTTCCCAGGTGATGACCTACCAGTTATCCAGGGTTCTGCTCTTAAAGCTCTTGAAGGCGAGCCAGAGTGGGAAGC
TAAGATCCTTGAGCTAGCTGAAGCTCTAGATACTTACATTCCTGAGCCAGAGCGTGCTATCGATGGTGCATTCATTCTTC
CAATCGAAGATGTATTCTCAATCGCTGGTCGTGGTACTGTTGTTACTGGTCGTGTAGAGCGCGGTATCATCAAAGTTGGT
GAAGAAGTAGAAATCGTTGGTATCAAAGATACAACTAAGTCTACTTGTACTGGTGTTGAAATGTTCCGTAAGCTTCTTGA
CGAAGGTCGTGCTGGCGAGAACTGTGGTGTTCTTCTACGTGGTATCAAGCGTGAAGATGTTGAGCGTGGTCAAGTACTAG
CTGCTCCAGGTTCAATCACTCCACACACTACTTTCAAGTCAGAAATCTATGTTCTGTCTAAAGAAGAAGGCGGACGTCAC
ACTCCATTCTTCAAAGGCTACCGTCCACAGTTCTACTTCCGTACAACTGACGTAACCGGTACTATCGAATTGCCAGAAGG
CGTTGAGATGGTAATGCCTGGTGACAACGTAGCTATGACTGTAACTCTAATCTGCCCAATCGCGATGGACGAAGGTTTAC
GCTTCGCTATCCGTGAAGGTGGCCGTACAGTTGGTGCTGGTGTTGTAGCTGAGATCGTTGCTTAA

Upstream 100 bases:

>100_bases
TAGAGGCCAGAGGCTAAACTGTCTCGCTCTATTGTTGATATACTTAATTGCTATTGCTCGGTTGTAAGCCGAGCATTTTC
TGAAAAGAAAGGAATATATC

Downstream 100 bases:

>100_bases
TTAGCAACGAGTCTTAACTCGATACTGAAAAAGGCAGCATAGGCTGCCTTTTTTATTGCCTGAAGAAAAGTGAGGCGAGG
GCTAGCAATCCAGATCGGAC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERGITINTSHIEYDTPSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIIKVG
EEVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSITPHTTFKSEIYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMTVTLICPIAMDEGLRFAIREGGRTVGAGVVAEIVA

Sequences:

>Translated_394_residues
MAKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERGITINTSHIEYDTPSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIIKVG
EEVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSITPHTTFKSEIYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMTVTLICPIAMDEGLRFAIREGGRTVGAGVVAEIVA
>Mature_393_residues
AKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERGITINTSHIEYDTPSRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEY
DFPGDDLPVIQGSALKALEGEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIIKVGE
EVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSITPHTTFKSEIYVLSKEEGGRHT
PFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMTVTLICPIAMDEGLRFAIREGGRTVGAGVVAEIVA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=54.6835443037975, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=29.1571753986333, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=29.1571753986333, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=28.6353467561521, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=28.4116331096197, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=29.2753623188406, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI194018522, Length=345, Percent_Identity=30.4347826086957, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI194097354, Length=345, Percent_Identity=30.4347826086957, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI194018520, Length=345, Percent_Identity=30.4347826086957, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI46094014, Length=448, Percent_Identity=27.6785714285714, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI4503507, Length=286, Percent_Identity=27.972027972028, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI53729339, Length=233, Percent_Identity=30.0429184549356, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=233, Percent_Identity=30.0429184549356, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=158, Percent_Identity=31.0126582278481, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=86.5482233502538, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=86.5482233502538, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=29.973474801061, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=29.7619047619048, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789108, Length=432, Percent_Identity=25.9259259259259, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.3809523809524, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=45.7142857142857, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=456, Percent_Identity=29.3859649122807, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=456, Percent_Identity=29.3859649122807, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=442, Percent_Identity=27.8280542986425, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=433, Percent_Identity=27.2517321016166, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=27.2093023255814, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=28.7822878228782, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=26.0623229461756, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=161, Percent_Identity=35.4037267080745, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=27.9569892473118, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=62.7848101265823, Blast_Score=505, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=29.5045045045045, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=29.5045045045045, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=457, Percent_Identity=24.2888402625821, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=29.7687861271676, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=279, Percent_Identity=26.8817204301075, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=236, Percent_Identity=28.3898305084746, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=55.012853470437, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=55.012853470437, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.0362694300518, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=27.6315789473684, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=27.6315789473684, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=27.6315789473684, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=27.6315789473684, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=27.8508771929825, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=27.8508771929825, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.4701986754967, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI17137380, Length=335, Percent_Identity=29.2537313432836, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45550900, Length=339, Percent_Identity=28.9085545722714, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24647048, Length=266, Percent_Identity=26.6917293233083, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24647046, Length=266, Percent_Identity=28.1954887218045, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28572034, Length=234, Percent_Identity=31.1965811965812, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43160; Mature: 43029

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERG
CCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHCC
ITINTSHIEYDTPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIIKVG
CCCCHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHHHCCCCEEEECHHHCCCEECC
EEVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSIT
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHCCCEEEECCCCCC
PHTTFKSEIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMT
CCCEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCEEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAEIVA
EEEEEEEHHCCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKEKFERVKPHVNVGTIGHVDHGKTTLTAAISSVLTKTYGGTKRDFAQIDNAPEERERG
CHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHCC
ITINTSHIEYDTPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVVASTDGPMPQTREHI
EEEEECEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPFIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GEPEWEAKILELAEALDTYIPEPERAIDGAFILPIEDVFSIAGRGTVVTGRVERGIIKVG
CCCCHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHHHCCCCEEEECHHHCCCEECC
EEVEIVGIKDTTKSTCTGVEMFRKLLDEGRAGENCGVLLRGIKREDVERGQVLAAPGSIT
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHCCCEEEECCCCCC
PHTTFKSEIYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNVAMT
CCCEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCEEEE
VTLICPIAMDEGLRFAIREGGRTVGAGVVAEIVA
EEEEEEEHHCCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA