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The map label for this gene is kbl [C]

Identifier: 157959937

GI number: 157959937

Start: 120102

End: 121298

Strand: Reverse

Name: kbl [C]

Synonym: Spea_0107

Alternate gene names: 157959937

Gene position: 121298-120102 (Counterclockwise)

Preceding gene: 157959938

Following gene: 157959936

Centisome position: 2.34

GC content: 47.7

Gene sequence:

>1197_bases
GTGCCTAAGACCTCATTCTACGACCAAATAAATCAACAGCTTGTCGACGTTAAAGCCGAAGGTTTGTATAAAAGCGAACG
AATCATTGTTTCTCCTCAACAAACTGCAATTAAAGTTAATGGCGACCAAGTTATTAATTTTTGTGCCAACAACTACCTAG
GTTTAGCTAACCACCCAGAGCTTATTAAGGCAGCACAAGCTGGCCTAGACGCTCACGGTTTTGGCATGGCATCTGTGCGC
TTTATCTGCGGCACCCAAGATATCCATAAGCAACTAGAAGCGAGCCTTAGCGAATTTTTAGGCATGGAAGACACCATTCT
TTACTCTTCATGCTTCGACGCTAACGCCGGTCTTTTCGAAACCCTATTAAGCGCAGAAGACGCGATTATTTCTGATGCAC
TAAACCACGCATCTATTATCGATGGCGTGCGTCTATGTAAGGCGAAGCGCTTCCGCTACGCTAACAACGACATGGCAGAT
TTAGAGGCGCAACTTATCGCCGCTAAAGAAGCTGGCGCTCGCAACATCATGATCGCTACCGACGGCGTGTTCTCAATGGA
CGGCGTAATTGCCAACCTAAAAGGTGTGTGTGATCTTGCTGACAAGTACGGCGCACTCGTGATGGTTGATGACTCACATG
CCGTAGGCTTTGTTGGCCAAGAAGGCCGTGGTACTCACGAGTATTGTGAAGTGATGGATCGCGTAGACATCATCACAGGT
ACTTTAGGTAAAGCCCTTGGTGGTGCATCAGGTGGATTCACTTCTGGTAAAAAAGAAGTCATCGACTGGTTGCGTCAACG
CTCTCGCCCTTACCTATTCTCAAACTCATTAGCGCCATCAATCGTCACGGCATCTATCCATGTACTTGAGATGCTCAAAT
CTGGCCAAGCGCTACGCGAAGCTGTTTGGGAAAATAGCCGTTACTTCCGCGAGCAGATGTCTGCGGCAGGCTTCACCCTA
GGTGGCGCCGATCACGCCATTATTCCTGTAATGATTGGTGACGCAAAACTGGCTGGCGATTTTGCTAACCGTCTATTAAC
TGAAAACATCTACGTTATCGGTTTCTCATTCCCTGTGGTACCAAAGGGTCAGGCACGTATTCGAACTCAGATGTCTGCAG
CTCACACCAAAGAGCAGATCGACAAGGCAATTGAAGCCTTTACCCGTATCGCGAAAGAGATGGGTATCATCAGCTAA

Upstream 100 bases:

>100_bases
CCCGGTTTTAGTCTTAAAAAAAGGCTGTAGAGCCGCGGCAATTAGGGTTAAAATGCCAGCACTCCAATACTCAAAATAAT
GCTAATTAAGGAGAATAAAG

Downstream 100 bases:

>100_bases
AATCAAATCAATAGGGGCTACGTATGTAGCCCTTTTTGAATGACATTCAGCAAGCCGCAAGCCGGCCTGTAATAGGTTAT
AAAATGAAAGCACTCAGCAA

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MPKTSFYDQINQQLVDVKAEGLYKSERIIVSPQQTAIKVNGDQVINFCANNYLGLANHPELIKAAQAGLDAHGFGMASVR
FICGTQDIHKQLEASLSEFLGMEDTILYSSCFDANAGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRFRYANNDMAD
LEAQLIAAKEAGARNIMIATDGVFSMDGVIANLKGVCDLADKYGALVMVDDSHAVGFVGQEGRGTHEYCEVMDRVDIITG
TLGKALGGASGGFTSGKKEVIDWLRQRSRPYLFSNSLAPSIVTASIHVLEMLKSGQALREAVWENSRYFREQMSAAGFTL
GGADHAIIPVMIGDAKLAGDFANRLLTENIYVIGFSFPVVPKGQARIRTQMSAAHTKEQIDKAIEAFTRIAKEMGIIS

Sequences:

>Translated_398_residues
MPKTSFYDQINQQLVDVKAEGLYKSERIIVSPQQTAIKVNGDQVINFCANNYLGLANHPELIKAAQAGLDAHGFGMASVR
FICGTQDIHKQLEASLSEFLGMEDTILYSSCFDANAGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRFRYANNDMAD
LEAQLIAAKEAGARNIMIATDGVFSMDGVIANLKGVCDLADKYGALVMVDDSHAVGFVGQEGRGTHEYCEVMDRVDIITG
TLGKALGGASGGFTSGKKEVIDWLRQRSRPYLFSNSLAPSIVTASIHVLEMLKSGQALREAVWENSRYFREQMSAAGFTL
GGADHAIIPVMIGDAKLAGDFANRLLTENIYVIGFSFPVVPKGQARIRTQMSAAHTKEQIDKAIEAFTRIAKEMGIIS
>Mature_397_residues
PKTSFYDQINQQLVDVKAEGLYKSERIIVSPQQTAIKVNGDQVINFCANNYLGLANHPELIKAAQAGLDAHGFGMASVRF
ICGTQDIHKQLEASLSEFLGMEDTILYSSCFDANAGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRFRYANNDMADL
EAQLIAAKEAGARNIMIATDGVFSMDGVIANLKGVCDLADKYGALVMVDDSHAVGFVGQEGRGTHEYCEVMDRVDIITGT
LGKALGGASGGFTSGKKEVIDWLRQRSRPYLFSNSLAPSIVTASIHVLEMLKSGQALREAVWENSRYFREQMSAAGFTLG
GADHAIIPVMIGDAKLAGDFANRLLTENIYVIGFSFPVVPKGQARIRTQMSAAHTKEQIDKAIEAFTRIAKEMGIIS

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=387, Percent_Identity=54.5219638242894, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI284448556, Length=416, Percent_Identity=50.7211538461538, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI83977442, Length=344, Percent_Identity=35.1744186046512, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI83977440, Length=344, Percent_Identity=35.1744186046512, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI83977444, Length=344, Percent_Identity=35.1744186046512, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI4502025, Length=363, Percent_Identity=33.0578512396694, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI40316939, Length=363, Percent_Identity=33.0578512396694, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI4758668, Length=358, Percent_Identity=36.5921787709497, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI119220554, Length=368, Percent_Identity=33.4239130434783, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI5454084, Length=261, Percent_Identity=31.4176245210728, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1790046, Length=397, Percent_Identity=75.8186397984887, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1786993, Length=347, Percent_Identity=33.7175792507205, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71994529, Length=399, Percent_Identity=55.1378446115288, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI32566772, Length=371, Percent_Identity=32.3450134770889, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17560912, Length=361, Percent_Identity=33.7950138504155, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17560914, Length=361, Percent_Identity=33.7950138504155, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71982617, Length=380, Percent_Identity=23.1578947368421, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982625, Length=380, Percent_Identity=23.1578947368421, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320438, Length=378, Percent_Identity=32.8042328042328, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6320267, Length=373, Percent_Identity=30.2949061662198, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6323954, Length=264, Percent_Identity=28.7878787878788, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24662918, Length=390, Percent_Identity=57.948717948718, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17137420, Length=349, Percent_Identity=32.6647564469914, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17136286, Length=411, Percent_Identity=32.360097323601, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24653280, Length=392, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653276, Length=392, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653278, Length=392, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43129; Mature: 42998

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTSFYDQINQQLVDVKAEGLYKSERIIVSPQQTAIKVNGDQVINFCANNYLGLANHPE
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECHHHHHHHHHCCCCCCCCCHH
LIKAAQAGLDAHGFGMASVRFICGTQDIHKQLEASLSEFLGMEDTILYSSCFDANAGLFE
HHHHHHCCCCCCCCCHHHEEEEECCHHHHHHHHHHHHHHCCCCHHEEEHHCCCCCCHHHH
TLLSAEDAIISDALNHASIIDGVRLCKAKRFRYANNDMADLEAQLIAAKEAGARNIMIAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEE
DGVFSMDGVIANLKGVCDLADKYGALVMVDDSHAVGFVGQEGRGTHEYCEVMDRVDIITG
CCCEEHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHH
TLGKALGGASGGFTSGKKEVIDWLRQRSRPYLFSNSLAPSIVTASIHVLEMLKSGQALRE
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHH
AVWENSRYFREQMSAAGFTLGGADHAIIPVMIGDAKLAGDFANRLLTENIYVIGFSFPVV
HHHCCHHHHHHHHHHCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCC
PKGQARIRTQMSAAHTKEQIDKAIEAFTRIAKEMGIIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PKTSFYDQINQQLVDVKAEGLYKSERIIVSPQQTAIKVNGDQVINFCANNYLGLANHPE
CCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECHHHHHHHHHCCCCCCCCCHH
LIKAAQAGLDAHGFGMASVRFICGTQDIHKQLEASLSEFLGMEDTILYSSCFDANAGLFE
HHHHHHCCCCCCCCCHHHEEEEECCHHHHHHHHHHHHHHCCCCHHEEEHHCCCCCCHHHH
TLLSAEDAIISDALNHASIIDGVRLCKAKRFRYANNDMADLEAQLIAAKEAGARNIMIAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEE
DGVFSMDGVIANLKGVCDLADKYGALVMVDDSHAVGFVGQEGRGTHEYCEVMDRVDIITG
CCCEEHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHH
TLGKALGGASGGFTSGKKEVIDWLRQRSRPYLFSNSLAPSIVTASIHVLEMLKSGQALRE
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHH
AVWENSRYFREQMSAAGFTLGGADHAIIPVMIGDAKLAGDFANRLLTENIYVIGFSFPVV
HHHCCHHHHHHHHHHCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCC
PKGQARIRTQMSAAHTKEQIDKAIEAFTRIAKEMGIIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]