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The map label for this gene is glpE [H]

Identifier: 157959935

GI number: 157959935

Start: 118577

End: 118885

Strand: Reverse

Name: glpE [H]

Synonym: Spea_0105

Alternate gene names: 157959935

Gene position: 118885-118577 (Counterclockwise)

Preceding gene: 157959936

Following gene: 157959934

Centisome position: 2.3

GC content: 43.04

Gene sequence:

>309_bases
ATGTCTGCTTTTCAACATCTTAGTGTTAATCAACTTATTCAGATGCAGTCTGAATCAGACGAGTTACAAATCGTGGATAT
CCGAGATGCAGCCAGCTATGAAGCCGGTCATATCCTTGGTTCAGTCAATCTAACCAATGAAAACTTAGCTCGCTTTATTG
GCGATGCCGACATGGATCTGCCACTTATTGTTGTTTGCTATCATGGCATCAGCAGCCAAAGCGCTGCCACTTATCTTAAT
GAGCAAGGCTTTGATGACGTGTTTAGTTTAGATGGCGGCTATAGCGCGTGGCATCAAGCTAACTTATAA

Upstream 100 bases:

>100_bases
GCCAGCTTTTGATTTATTCTCCGAACAAGTGACTGTAATATCTGCTTGAGACTATTACAGTCGCTTTTGTCTTCTCTAAT
CTAATCAATACAGGTTTTCT

Downstream 100 bases:

>100_bases
ACATGTAAGAGGGGGAGAAAATGATTGAGATTGGCAGACTTCCTAACAACCGAGCAGCACAGGCTCTCATTGACTACCTC
AAAGGGCTAAATATTGATTG

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 102; Mature: 101

Protein sequence:

>102_residues
MSAFQHLSVNQLIQMQSESDELQIVDIRDAASYEAGHILGSVNLTNENLARFIGDADMDLPLIVVCYHGISSQSAATYLN
EQGFDDVFSLDGGYSAWHQANL

Sequences:

>Translated_102_residues
MSAFQHLSVNQLIQMQSESDELQIVDIRDAASYEAGHILGSVNLTNENLARFIGDADMDLPLIVVCYHGISSQSAATYLN
EQGFDDVFSLDGGYSAWHQANL
>Mature_101_residues
SAFQHLSVNQLIQMQSESDELQIVDIRDAASYEAGHILGSVNLTNENLARFIGDADMDLPLIVVCYHGISSQSAATYLNE
QGFDDVFSLDGGYSAWHQANL

Specific function: Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide [H]

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1789831, Length=99, Percent_Identity=43.4343434343434, Blast_Score=90, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 11193; Mature: 11062

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFQHLSVNQLIQMQSESDELQIVDIRDAASYEAGHILGSVNLTNENLARFIGDADMDL
CCCCHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEECHHHHHHHHCCCCCCC
PLIVVCYHGISSQSAATYLNEQGFDDVFSLDGGYSAWHQANL
CEEEEEECCCCCCCHHHHHCCCCCCCEEECCCCCCCCEECCC
>Mature Secondary Structure 
SAFQHLSVNQLIQMQSESDELQIVDIRDAASYEAGHILGSVNLTNENLARFIGDADMDL
CCCHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEECHHHHHHHHCCCCCCC
PLIVVCYHGISSQSAATYLNEQGFDDVFSLDGGYSAWHQANL
CEEEEEECCCCCCCHHHHHCCCCCCCEEECCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]