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The map label for this gene is hemN [H]

Identifier: 157959926

GI number: 157959926

Start: 107416

End: 108756

Strand: Reverse

Name: hemN [H]

Synonym: Spea_0096

Alternate gene names: 157959926

Gene position: 108756-107416 (Counterclockwise)

Preceding gene: 157959927

Following gene: 157959925

Centisome position: 2.1

GC content: 44.59

Gene sequence:

>1341_bases
ATGATCGAAAAGTACAATTATAGCGGTCCACGTTATACTTCTTATCCGACGGCCTTGGAATTCGATGACTCGTTTACCGA
GGAAAAATTGCTGACTTCTATCAAGAACAGCAAGAGTGACAAACTTTCTCTTTATATCCATATTCCTTTCTGCGCCAAGC
TTTGTTACTACTGTGGCTGCAATAAAGTTATCACTCGTCATCAGCATAAAGCCGATCAGTACATTGAGTACTTAGCAGCA
GAAATCGTTAAACGTGCACCGCTGTTTAAAAATTACTTAGTTACCCAAATTCACTGGGGTGGTGGTACACCGACATTCCT
AACTCCTGAGCAAATTCTTAAGCTATCAGCACTCATTAAGGCTAACTTCAACGTTGCCGATGTAGGTGAATACTCAATCG
AAGTCGACCCGCGTGAAATCGAACTTTCGATGCTAGACACCCTAAAAGAAGCCGGCTTCAACCGTATCTCTATCGGTGTT
CAAGACTTCAACAAGAAAGTGCAAGTTGCAGTAAACCGTGAGCAAGACGAGCAGTTTATCTTCGACCTAATGGCACGCGC
TAAAGAGCTAGGCTTTGTTTCAACTAACATCGACTTAATCTACGGCTTGCCGCACCAGACACCAGAGACCTTTGCTGAAA
CCATGCAGCGCGTACTGGATCTAAACCCAGACCGTCTATCTGTATTTAACTACGCTCACCTGCCATCACGTTTTGCAGCC
CAGCGTAAGATTAAAGACGAAGACTTAGCGTCACCACAGCAGAAGCTTGATATGTTACACCAAACTATCGAGACGCTAAC
GGGCGCAGGTTACCAATACATTGGTATGGACCACTTTGCTAAGCCAGATGACGAGCTGTCTATTCTGCAAAACGAAGGTC
GTCTACACCGTAACTTCCAAGGTTACACGACTCAAGAAGAGTGTGATCTATTGGGTCTAGGTGTGTCTTCAATCAGCCAA
ATTGGCGATTGCTACGCACAGAACCAGAAAGACATTCGTCCATACTTCGAGTCAATCGATGCTAATGGTCATGCACTCTG
GAAAGGTTGTAGCTTAAACCGTGACGACGAAATTCGCCGCGTAGTGATTAAGCAGCTTATCTGCCACTTCGACCTAGATA
TGGCGAAGATTGAAGAGAAGCTAAACATCAACTTCGAAGAGTATTTCGCAGAAGACTTAAAGCTACTGCAAACCTTCATC
GATGATAAGCTAGTCGACATAACAGACCGCAAGATCACTATCAGCCCTACTGGCCGCCTGTTGATCCGTAACATCTGTAT
CTGTTTCGATGTTTACTACCGTGAGAAAGCAAGACAACAGCAGTTCTCTCGCGTTATCTAA

Upstream 100 bases:

>100_bases
GGTTATTCAACAAATAACCGTGCATACTTCACATGTTGCTAAACTACACGCCGGAGGACACGCCTTGAAGCAGCCCACCC
CAATTAGTTGGGATCAGTCA

Downstream 100 bases:

>100_bases
TCGGCTTGTAAAGTACAATTAGAACCTCAAAAAACCGGCCTTAGCCGGTTTTTTTGTGCCTGATAAATATAATACCAATC
AGTATAAGAAGTTGATCTAC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MIEKYNYSGPRYTSYPTALEFDDSFTEEKLLTSIKNSKSDKLSLYIHIPFCAKLCYYCGCNKVITRHQHKADQYIEYLAA
EIVKRAPLFKNYLVTQIHWGGGTPTFLTPEQILKLSALIKANFNVADVGEYSIEVDPREIELSMLDTLKEAGFNRISIGV
QDFNKKVQVAVNREQDEQFIFDLMARAKELGFVSTNIDLIYGLPHQTPETFAETMQRVLDLNPDRLSVFNYAHLPSRFAA
QRKIKDEDLASPQQKLDMLHQTIETLTGAGYQYIGMDHFAKPDDELSILQNEGRLHRNFQGYTTQEECDLLGLGVSSISQ
IGDCYAQNQKDIRPYFESIDANGHALWKGCSLNRDDEIRRVVIKQLICHFDLDMAKIEEKLNINFEEYFAEDLKLLQTFI
DDKLVDITDRKITISPTGRLLIRNICICFDVYYREKARQQQFSRVI

Sequences:

>Translated_446_residues
MIEKYNYSGPRYTSYPTALEFDDSFTEEKLLTSIKNSKSDKLSLYIHIPFCAKLCYYCGCNKVITRHQHKADQYIEYLAA
EIVKRAPLFKNYLVTQIHWGGGTPTFLTPEQILKLSALIKANFNVADVGEYSIEVDPREIELSMLDTLKEAGFNRISIGV
QDFNKKVQVAVNREQDEQFIFDLMARAKELGFVSTNIDLIYGLPHQTPETFAETMQRVLDLNPDRLSVFNYAHLPSRFAA
QRKIKDEDLASPQQKLDMLHQTIETLTGAGYQYIGMDHFAKPDDELSILQNEGRLHRNFQGYTTQEECDLLGLGVSSISQ
IGDCYAQNQKDIRPYFESIDANGHALWKGCSLNRDDEIRRVVIKQLICHFDLDMAKIEEKLNINFEEYFAEDLKLLQTFI
DDKLVDITDRKITISPTGRLLIRNICICFDVYYREKARQQQFSRVI
>Mature_446_residues
MIEKYNYSGPRYTSYPTALEFDDSFTEEKLLTSIKNSKSDKLSLYIHIPFCAKLCYYCGCNKVITRHQHKADQYIEYLAA
EIVKRAPLFKNYLVTQIHWGGGTPTFLTPEQILKLSALIKANFNVADVGEYSIEVDPREIELSMLDTLKEAGFNRISIGV
QDFNKKVQVAVNREQDEQFIFDLMARAKELGFVSTNIDLIYGLPHQTPETFAETMQRVLDLNPDRLSVFNYAHLPSRFAA
QRKIKDEDLASPQQKLDMLHQTIETLTGAGYQYIGMDHFAKPDDELSILQNEGRLHRNFQGYTTQEECDLLGLGVSSISQ
IGDCYAQNQKDIRPYFESIDANGHALWKGCSLNRDDEIRRVVIKQLICHFDLDMAKIEEKLNINFEEYFAEDLKLLQTFI
DDKLVDITDRKITISPTGRLLIRNICICFDVYYREKARQQQFSRVI

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=245, Percent_Identity=28.1632653061224, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI87082341, Length=446, Percent_Identity=65.0224215246637, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1789325, Length=247, Percent_Identity=31.1740890688259, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 51491; Mature: 51491

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKYNYSGPRYTSYPTALEFDDSFTEEKLLTSIKNSKSDKLSLYIHIPFCAKLCYYCGC
CCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHCC
NKVITRHQHKADQYIEYLAAEIVKRAPLFKNYLVTQIHWGGGTPTFLTPEQILKLSALIK
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ANFNVADVGEYSIEVDPREIELSMLDTLKEAGFNRISIGVQDFNKKVQVAVNREQDEQFI
CCCCEEECCCEEEEECHHHHHHHHHHHHHHCCCCEEEEEHHHCCCEEEEEECCCCHHHHH
FDLMARAKELGFVSTNIDLIYGLPHQTPETFAETMQRVLDLNPDRLSVFNYAHLPSRFAA
HHHHHHHHHCCEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHCCHHHHH
QRKIKDEDLASPQQKLDMLHQTIETLTGAGYQYIGMDHFAKPDDELSILQNEGRLHRNFQ
HHCCCCHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCC
GYTTQEECDLLGLGVSSISQIGDCYAQNQKDIRPYFESIDANGHALWKGCSLNRDDEIRR
CCCCHHHCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHH
VVIKQLICHFDLDMAKIEEKLNINFEEYFAEDLKLLQTFIDDKLVDITDRKITISPTGRL
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHH
LIRNICICFDVYYREKARQQQFSRVI
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEKYNYSGPRYTSYPTALEFDDSFTEEKLLTSIKNSKSDKLSLYIHIPFCAKLCYYCGC
CCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHCC
NKVITRHQHKADQYIEYLAAEIVKRAPLFKNYLVTQIHWGGGTPTFLTPEQILKLSALIK
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ANFNVADVGEYSIEVDPREIELSMLDTLKEAGFNRISIGVQDFNKKVQVAVNREQDEQFI
CCCCEEECCCEEEEECHHHHHHHHHHHHHHCCCCEEEEEHHHCCCEEEEEECCCCHHHHH
FDLMARAKELGFVSTNIDLIYGLPHQTPETFAETMQRVLDLNPDRLSVFNYAHLPSRFAA
HHHHHHHHHCCEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHCCHHHHH
QRKIKDEDLASPQQKLDMLHQTIETLTGAGYQYIGMDHFAKPDDELSILQNEGRLHRNFQ
HHCCCCHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCC
GYTTQEECDLLGLGVSSISQIGDCYAQNQKDIRPYFESIDANGHALWKGCSLNRDDEIRR
CCCCHHHCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHH
VVIKQLICHFDLDMAKIEEKLNINFEEYFAEDLKLLQTFIDDKLVDITDRKITISPTGRL
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHH
LIRNICICFDVYYREKARQQQFSRVI
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7768836; 8346018; 9278503 [H]