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The map label for this gene is fliA [H]

Identifier: 157959887

GI number: 157959887

Start: 71412

End: 72134

Strand: Reverse

Name: fliA [H]

Synonym: Spea_0057

Alternate gene names: 157959887

Gene position: 72134-71412 (Counterclockwise)

Preceding gene: 157959888

Following gene: 157959886

Centisome position: 1.39

GC content: 45.23

Gene sequence:

>723_bases
ATGAACACTGCACTATCTGCCTATCAAGAAGCTGCTGAAGAAGTTGCGATTAACCGTCTCGCCGAAAGTCAGGTGATGAA
GCAATACCTCCCTTTAGTTAAGCGTTCCGTATCTCAACTTAGAAGCCATTGTGGTATTGCGCTCGCGATAGAAGATATGG
AACAGATAGGCATGATGGCGCTATTAGAGTCAGCCCGTCGCTACCCAGGTGAGTACGACAATGGCTTTTTGTCTTTTGCA
GGACAACGTATTCGTGGCGCGATTCTAGACGAGCTACGCCGCCAAGATTGGCGGCCACGTCCCGTTAGGCAGCAAGCCCA
CGAACTTAACGATATGGTACGAAAACTCACTCGTTCGTTAAAGCGAGAACCCACTGATTTGGAGGTTGCACAATCTATGG
GACTTAGCCAAGTGGAGTATCGAGAACGCCTATTTGCATCTCAGTCTGAATCGATGCGTAGCTTGGACGAACTCATTGGT
GACGGCGGACACTTTGAAGATAAAAGTGACATGTTGGAGCAATTTTCAACAAAAGAAACGTTATTCCAAGCTATTTTAAA
ATTAAACAAAAGAGAACAGATAATTCTGTCGCTTTACTATCAGCATGAACTCAATTTAAAAGAAATCGCCGCAACATTAG
GACTGACTGAGACACGTATTTGCCAGCTGCACAAACACGCAGTGAAACAACTTCAGCAGATCTATCAAGAATGGGAGCGG
TAA

Upstream 100 bases:

>100_bases
CCCACAGTATCTTATCTGCAGTGCTAGCACAAAATGACTTCAATATAGAGTTAGAAGAAGTGCTGTTTACTCGAATGATT
ATTCAATAGAAGGCTATGCT

Downstream 100 bases:

>100_bases
ATACGTGAGGTAGTATGAGTAAACTTATAGGGTTAGTGATAATTCTGTTATCTGTGTTTGGTGGTTATATGTGGGCTGGC
GGCGTATTACTCGCGCTATG

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-F factor [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNTALSAYQEAAEEVAINRLAESQVMKQYLPLVKRSVSQLRSHCGIALAIEDMEQIGMMALLESARRYPGEYDNGFLSFA
GQRIRGAILDELRRQDWRPRPVRQQAHELNDMVRKLTRSLKREPTDLEVAQSMGLSQVEYRERLFASQSESMRSLDELIG
DGGHFEDKSDMLEQFSTKETLFQAILKLNKREQIILSLYYQHELNLKEIAATLGLTETRICQLHKHAVKQLQQIYQEWER

Sequences:

>Translated_240_residues
MNTALSAYQEAAEEVAINRLAESQVMKQYLPLVKRSVSQLRSHCGIALAIEDMEQIGMMALLESARRYPGEYDNGFLSFA
GQRIRGAILDELRRQDWRPRPVRQQAHELNDMVRKLTRSLKREPTDLEVAQSMGLSQVEYRERLFASQSESMRSLDELIG
DGGHFEDKSDMLEQFSTKETLFQAILKLNKREQIILSLYYQHELNLKEIAATLGLTETRICQLHKHAVKQLQQIYQEWER
>Mature_240_residues
MNTALSAYQEAAEEVAINRLAESQVMKQYLPLVKRSVSQLRSHCGIALAIEDMEQIGMMALLESARRYPGEYDNGFLSFA
GQRIRGAILDELRRQDWRPRPVRQQAHELNDMVRKLTRSLKREPTDLEVAQSMGLSQVEYRERLFASQSESMRSLDELIG
DGGHFEDKSDMLEQFSTKETLFQAILKLNKREQIILSLYYQHELNLKEIAATLGLTETRICQLHKHAVKQLQQIYQEWER

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=216, Percent_Identity=36.1111111111111, Blast_Score=141, Evalue=3e-35,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27857; Mature: 27857

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALSAYQEAAEEVAINRLAESQVMKQYLPLVKRSVSQLRSHCGIALAIEDMEQIGMMA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLESARRYPGEYDNGFLSFAGQRIRGAILDELRRQDWRPRPVRQQAHELNDMVRKLTRSL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
KREPTDLEVAQSMGLSQVEYRERLFASQSESMRSLDELIGDGGHFEDKSDMLEQFSTKET
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LFQAILKLNKREQIILSLYYQHELNLKEIAATLGLTETRICQLHKHAVKQLQQIYQEWER
HHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTALSAYQEAAEEVAINRLAESQVMKQYLPLVKRSVSQLRSHCGIALAIEDMEQIGMMA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLESARRYPGEYDNGFLSFAGQRIRGAILDELRRQDWRPRPVRQQAHELNDMVRKLTRSL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
KREPTDLEVAQSMGLSQVEYRERLFASQSESMRSLDELIGDGGHFEDKSDMLEQFSTKET
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LFQAILKLNKREQIILSLYYQHELNLKEIAATLGLTETRICQLHKHAVKQLQQIYQEWER
HHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501040; 12620739 [H]