Click here to switch to the map view.

The map label for this gene is 157959879

Identifier: 157959879

GI number: 157959879

Start: 65250

End: 66323

Strand: Direct

Name: 157959879

Synonym: Spea_0049

Alternate gene names: NA

Gene position: 65250-66323 (Clockwise)

Preceding gene: 157959878

Following gene: 157959880

Centisome position: 1.26

GC content: 42.74

Gene sequence:

>1074_bases
ATGACGCTAATTATTGCAGAAGCTGGTGTGAATCATAACGGTGACGAGAAATTGGCTTTTGAATTAGTCAGTGCGGCGCA
TCAGGCGGGTGCTGACATTGTTAAGTTTCAGACTTTTAAAGCTAAAAATCTCGTTACGGAAGATGCTACTCAAGCTGAAT
ACCAAATTACCAATACACAGGTTCAGGAGTCGCAGCTACTTATGCTGAGTAGACTTGAACTTTCCTATGAGACTCACCAT
AAGTTAGTCCAGTATTGTGCTGAGTTGGGTATCGAGTTCCTTTCTACAGCCTTTGATTTTGAAAGCCTAGATTTTCTTGT
GAATGATTTAAGACTGAGGCGCTTAAAAATACCTTCTGGTGAGATTACAAACGCTCCATTGGTGCTTGAGCATGCTCGTA
CAGGGTGCGACTTAATTGTGTCGACAGGTATGGCAACTCTCTCAGAGATTGAGGCCACATTGGGTGTCATTGCATTTGGG
TATATCGATGATAAAGACATCGAGCCGAATGAGTTAGCATTTCAGCAAGCCTATGCATCAGTTCAAGGTCAGCAACTTCT
AAAGAACAAGGTGACAATACTACATTGCACTACAGAATACCCAGCACCGATGGCGGAAATTAATCTAAAGGTCATGGATA
CGTTAGGTCAAGCATTTGAGTTACCTGCTGGATATTCAGACCATAGTGAAGGTATAGCTATCCCCATTGCAGCAGTGGCT
CGTGGTGCGGTGTTGATTGAAAAACACTTTACATTAGATAGAAATATGCAAGGTCCCGATCATAAAGCCTCTTTAGAGCC
TCAAGAACTTACTGCCATGGTTAATGCAATACGCCAAGTTGAAGTGGCTTTGGGGCGGAGTGTAAAGTGCCCTACTGCAT
CAGAAGTTAAAAATAAGGATGTGGCGCGTAAAAGTTTAGTTGCGGCGAAGGTGATTAAGCAAGGCGAGTTAATTACAGAA
GAAAACCTTACGATAAAGCGCCCTGGTTCAGGTATGTCACCATATATGTATTGGAGGTTACTGAATAAGCCCGCAAGCAA
AAACTACCAAGCTGGGGACTTGATCTTTGAATAA

Upstream 100 bases:

>100_bases
AATGCGAGCAAACAAGTTATTGAAATGATAAAAACACTTTCATTTGAACCAAGTAAAGTGTTCTTCGATATCAACTTTTC
TTGCCAACAGTGAGGTCATC

Downstream 100 bases:

>100_bases
AGTTAAGGCCAAGCCCCTTGTTATTCTCGGTGGTGGAGGGCATGCCAGTGTATTGGTTGATATCTTGCTTTCACAACAAC
GTGAGATAGTCGCAATTGTC

Product: N-acylneuraminate-9-phosphate synthase

Products: phosphate; N-acetylneuraminate

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTLIIAEAGVNHNGDEKLAFELVSAAHQAGADIVKFQTFKAKNLVTEDATQAEYQITNTQVQESQLLMLSRLELSYETHH
KLVQYCAELGIEFLSTAFDFESLDFLVNDLRLRRLKIPSGEITNAPLVLEHARTGCDLIVSTGMATLSEIEATLGVIAFG
YIDDKDIEPNELAFQQAYASVQGQQLLKNKVTILHCTTEYPAPMAEINLKVMDTLGQAFELPAGYSDHSEGIAIPIAAVA
RGAVLIEKHFTLDRNMQGPDHKASLEPQELTAMVNAIRQVEVALGRSVKCPTASEVKNKDVARKSLVAAKVIKQGELITE
ENLTIKRPGSGMSPYMYWRLLNKPASKNYQAGDLIFE

Sequences:

>Translated_357_residues
MTLIIAEAGVNHNGDEKLAFELVSAAHQAGADIVKFQTFKAKNLVTEDATQAEYQITNTQVQESQLLMLSRLELSYETHH
KLVQYCAELGIEFLSTAFDFESLDFLVNDLRLRRLKIPSGEITNAPLVLEHARTGCDLIVSTGMATLSEIEATLGVIAFG
YIDDKDIEPNELAFQQAYASVQGQQLLKNKVTILHCTTEYPAPMAEINLKVMDTLGQAFELPAGYSDHSEGIAIPIAAVA
RGAVLIEKHFTLDRNMQGPDHKASLEPQELTAMVNAIRQVEVALGRSVKCPTASEVKNKDVARKSLVAAKVIKQGELITE
ENLTIKRPGSGMSPYMYWRLLNKPASKNYQAGDLIFE
>Mature_356_residues
TLIIAEAGVNHNGDEKLAFELVSAAHQAGADIVKFQTFKAKNLVTEDATQAEYQITNTQVQESQLLMLSRLELSYETHHK
LVQYCAELGIEFLSTAFDFESLDFLVNDLRLRRLKIPSGEITNAPLVLEHARTGCDLIVSTGMATLSEIEATLGVIAFGY
IDDKDIEPNELAFQQAYASVQGQQLLKNKVTILHCTTEYPAPMAEINLKVMDTLGQAFELPAGYSDHSEGIAIPIAAVAR
GAVLIEKHFTLDRNMQGPDHKASLEPQELTAMVNAIRQVEVALGRSVKCPTASEVKNKDVARKSLVAAKVIKQGELITEE
NLTIKRPGSGMSPYMYWRLLNKPASKNYQAGDLIFE

Specific function: Unknown

COG id: COG2089

COG function: function code M; Sialic acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis spsE [H]

Homologues:

Organism=Homo sapiens, GI12056473, Length=363, Percent_Identity=31.129476584022, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI21357081, Length=295, Percent_Identity=31.864406779661, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006190
- InterPro:   IPR013785
- InterPro:   IPR013132
- InterPro:   IPR013974 [H]

Pfam domain/function: PF03102 NeuB; PF08666 SAF [H]

EC number: 2.5.1.56

Molecular weight: Translated: 39320; Mature: 39189

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50844 AFP_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIIAEAGVNHNGDEKLAFELVSAAHQAGADIVKFQTFKAKNLVTEDATQAEYQITNTQ
CEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCCEEEECCCH
VQESQLLMLSRLELSYETHHKLVQYCAELGIEFLSTAFDFESLDFLVNDLRLRRLKIPSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCC
EITNAPLVLEHARTGCDLIVSTGMATLSEIEATLGVIAFGYIDDKDIEPNELAFQQAYAS
CCCCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHH
VQGQQLLKNKVTILHCTTEYPAPMAEINLKVMDTLGQAFELPAGYSDHSEGIAIPIAAVA
HHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHH
RGAVLIEKHFTLDRNMQGPDHKASLEPQELTAMVNAIRQVEVALGRSVKCPTASEVKNKD
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VARKSLVAAKVIKQGELITEENLTIKRPGSGMSPYMYWRLLNKPASKNYQAGDLIFE
HHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLIIAEAGVNHNGDEKLAFELVSAAHQAGADIVKFQTFKAKNLVTEDATQAEYQITNTQ
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCCEEEECCCH
VQESQLLMLSRLELSYETHHKLVQYCAELGIEFLSTAFDFESLDFLVNDLRLRRLKIPSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCC
EITNAPLVLEHARTGCDLIVSTGMATLSEIEATLGVIAFGYIDDKDIEPNELAFQQAYAS
CCCCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHH
VQGQQLLKNKVTILHCTTEYPAPMAEINLKVMDTLGQAFELPAGYSDHSEGIAIPIAAVA
HHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHH
RGAVLIEKHFTLDRNMQGPDHKASLEPQELTAMVNAIRQVEVALGRSVKCPTASEVKNKD
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VARKSLVAAKVIKQGELITEENLTIKRPGSGMSPYMYWRLLNKPASKNYQAGDLIFE
HHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; N-acetyl-D-mannosamine; H2O

Specific reaction: phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O = phosphate + N-acetylneuraminate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]