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The map label for this gene is aroE

Identifier: 157959870

GI number: 157959870

Start: 42821

End: 43636

Strand: Direct

Name: aroE

Synonym: Spea_0040

Alternate gene names: 157959870

Gene position: 42821-43636 (Clockwise)

Preceding gene: 157959868

Following gene: 157959871

Centisome position: 0.83

GC content: 45.96

Gene sequence:

>816_bases
ATGACCGAACGATATGCAGTATTTGGTAATCCAATTGGTCACAGTAAGTCGCCAGCCATACATTCCATGTTTGCCACAGA
AACGGCTCAGTCATTAACTTATGAGGCAATCTTAGCGCCAATTGATGCATTTGAAGCGACATTCAAAGAGTTTGTGACTA
ATAAGGGCTATGGAGCCAATGTTACCGTGCCTTTTAAAGAGCAAGCATTTGCACTTTGTGATGAGTTAAGTGAGCAAGCT
AAGCTAGCAGGCGCAGTAAACACTCTATCAGTACTGGCCGATGGCAAAATTCGTGGTGATAATACAGATGGGCTAGGCCT
AGTTGCCGATCTGCAACGAAACTTAGGCAGTCTTACAGGGCTTAAAGTTCTTCTGGTTGGGGCTGGTGGTGCGGCTCGGG
GTAGTGTGCTGCCATTATTGCAGGCAGGAATTGCCAAGCTTAGTATCGTTAACCGCACACAGGCGAAAGCAGAGATTCTG
GCTGAACTCTTTTCGAGCTATGGCGATGTGGTTTCTTTACCGATAAGTCATACGGGCAAAAGCTACGATGTGATTATTAA
TTCAACCTCCTCAAGTTTAAGTGGTGAGGTGCCCAATATCAGTAGCGATAGCATTACATCACAAACCGTTTGCTACGATA
TGATGTACGGCAAAGAGCCAACGTCATTTAATGTATGGGCTAAGGCGCAGGGTGCCAAACAGACCGTAGATGGTTTAGGC
ATGCTGGTGGGACAAGCTGCAGAAAGCTTTAATATTTGGCGCAAGGTAAGGCCAAGTGTTGAGCCTGTATTAACTCAGCT
TCGAGCCGAACTCTAA

Upstream 100 bases:

>100_bases
TAAGCTGAAATATAAAAGATGAAAATTACTCCAATAGAGACGATTGCCTAGCAGCGGAACCGAGTTACACTAGAGTTTTT
CGCGGCAAAGAGAAGTAAGC

Downstream 100 bases:

>100_bases
ATTGAGATAAGTTTGAGATAGAAATGAATCAAAGTATTTTATTCCCCGATCTACAAAATTGGGACTCACAAACACAAACC
GTGGTATTTCCTGTTCAGAT

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTERYAVFGNPIGHSKSPAIHSMFATETAQSLTYEAILAPIDAFEATFKEFVTNKGYGANVTVPFKEQAFALCDELSEQA
KLAGAVNTLSVLADGKIRGDNTDGLGLVADLQRNLGSLTGLKVLLVGAGGAARGSVLPLLQAGIAKLSIVNRTQAKAEIL
AELFSSYGDVVSLPISHTGKSYDVIINSTSSSLSGEVPNISSDSITSQTVCYDMMYGKEPTSFNVWAKAQGAKQTVDGLG
MLVGQAAESFNIWRKVRPSVEPVLTQLRAEL

Sequences:

>Translated_271_residues
MTERYAVFGNPIGHSKSPAIHSMFATETAQSLTYEAILAPIDAFEATFKEFVTNKGYGANVTVPFKEQAFALCDELSEQA
KLAGAVNTLSVLADGKIRGDNTDGLGLVADLQRNLGSLTGLKVLLVGAGGAARGSVLPLLQAGIAKLSIVNRTQAKAEIL
AELFSSYGDVVSLPISHTGKSYDVIINSTSSSLSGEVPNISSDSITSQTVCYDMMYGKEPTSFNVWAKAQGAKQTVDGLG
MLVGQAAESFNIWRKVRPSVEPVLTQLRAEL
>Mature_270_residues
TERYAVFGNPIGHSKSPAIHSMFATETAQSLTYEAILAPIDAFEATFKEFVTNKGYGANVTVPFKEQAFALCDELSEQAK
LAGAVNTLSVLADGKIRGDNTDGLGLVADLQRNLGSLTGLKVLLVGAGGAARGSVLPLLQAGIAKLSIVNRTQAKAEILA
ELFSSYGDVVSLPISHTGKSYDVIINSTSSSLSGEVPNISSDSITSQTVCYDMMYGKEPTSFNVWAKAQGAKQTVDGLGM
LVGQAAESFNIWRKVRPSVEPVLTQLRAEL

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=270, Percent_Identity=52.5925925925926, Blast_Score=275, Evalue=2e-75,
Organism=Escherichia coli, GI1787983, Length=280, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320332, Length=181, Percent_Identity=35.9116022099448, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_SHEPA (A8GYI2)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001499904.1
- ProteinModelPortal:   A8GYI2
- SMR:   A8GYI2
- STRING:   A8GYI2
- GeneID:   5660441
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_0040
- NMPDR:   fig|398579.3.peg.37
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   DQYVVFG
- ProtClustDB:   CLSK981509
- BioCyc:   SPEA398579:SPEA_0040-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 28680; Mature: 28549

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERYAVFGNPIGHSKSPAIHSMFATETAQSLTYEAILAPIDAFEATFKEFVTNKGYGAN
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
VTVPFKEQAFALCDELSEQAKLAGAVNTLSVLADGKIRGDNTDGLGLVADLQRNLGSLTG
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCC
LKVLLVGAGGAARGSVLPLLQAGIAKLSIVNRTQAKAEILAELFSSYGDVVSLPISHTGK
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
SYDVIINSTSSSLSGEVPNISSDSITSQTVCYDMMYGKEPTSFNVWAKAQGAKQTVDGLG
EEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
MLVGQAAESFNIWRKVRPSVEPVLTQLRAEL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TERYAVFGNPIGHSKSPAIHSMFATETAQSLTYEAILAPIDAFEATFKEFVTNKGYGAN
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
VTVPFKEQAFALCDELSEQAKLAGAVNTLSVLADGKIRGDNTDGLGLVADLQRNLGSLTG
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCC
LKVLLVGAGGAARGSVLPLLQAGIAKLSIVNRTQAKAEILAELFSSYGDVVSLPISHTGK
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
SYDVIINSTSSSLSGEVPNISSDSITSQTVCYDMMYGKEPTSFNVWAKAQGAKQTVDGLG
EEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
MLVGQAAESFNIWRKVRPSVEPVLTQLRAEL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA