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The map label for this gene is fadA [H]

Identifier: 157959847

GI number: 157959847

Start: 19009

End: 20172

Strand: Reverse

Name: fadA [H]

Synonym: Spea_0017

Alternate gene names: 157959847

Gene position: 20172-19009 (Counterclockwise)

Preceding gene: 157959848

Following gene: 157959846

Centisome position: 0.39

GC content: 49.4

Gene sequence:

>1164_bases
ATGAAAAACGCCGTTATCGTAGATTGCATTCGTACTCCAATGGGTCGCTCAAAGGCTGGAGTATTTAGAAATGTACGCGC
AGAAACTCTTTCTGCTGAACTAATGAAGTCGCTACTTGAGCGCAACCCAAAGCTTGACCCAAACACCATCGAAGATGTGA
TGTGGGGTTGTGTGCAGCAAACCCTAGAGCAAGGCTTTAATATTGCCCGTAACGCCGCCCTACTCGCTGGCATTCCAAAA
CAAGTCGGTGCTGTTACCGTAAACCGTTTATGTGGTTCATCTATGGATGCCCTACATCAGGCTGCTCGTGCGATTATGAC
TGGCCAAGGTGATACCTTTATTGTTGGTGGTGTTGAACACATGGGCCACGTACCGATGAACCATGGTGTCGACTTCCATC
CAGGTCTTGCTAATAACGTAGCAAAAGCTTCTGCCATGATGGGGCTTACAGCTGAGATGCTAGGTAAGATGCATGGAATT
ACTCGTGAGCAGCAAGATGCCTTTGCGGTTCGCTCGCATCAACGTGCTCACGCAGCTACAGTTGAAGGCCGCTTCGCCAA
TGAAATCGTAGCGATTGAAGGTCATGACGCCGATGGCGCATTGATTAAAGTCGAGCATGATGAAGTGATCCGCCCTGAAA
CCTCGATGGAATCGCTATCGGGTTTACGCCCAGCTTTTGACCCAGCTAACGGCACAGTTACCGCAGGTACCTCTTCTGCA
CTTTCAGATGGCGCCTCTGCCATGTTAGTCATGGAAGAGGAAAAAGCCAAAGCGTTAGGCCTGCCTATTCGTGCCCGTAT
CCGCTCTATGGCTATTGCCGGTTGTGATGCTGCTATTATGGGTTATGGCCCTGTACCTGCCACTCAAAAAGCCCTTAAGC
GTGCAGGCCTAACCGTTGATGACATGGATGTTATCGAGCTTAATGAAGCCTTCGCCGCTCAGTCTCTACCTTGTGTTAAA
GACCTAGGTTTGATGGATGTGGTCGAAGACAAAGTTAACCTCAACGGTGGTGCCATTGCACTAGGTCATCCACTAGGTTG
TTCAGGTACGCGTATTTCAACGACCTTGATTAACCTGATGGAAGCCAAAGATGCCAAATATGGTCTTGCGACCATGTGTA
TCGGTCTAGGCCAAGGTATCGCGACTATCTTCGAGCGCCCATAA

Upstream 100 bases:

>100_bases
ACACCTAGGTGGTCTATACCAAGTGACCGACACTATGCGCGAACTTGCCGCCAACAATGGCAGCTACTACCAGCAGGCTT
AAGCAGGAAGGATTAGAACA

Downstream 100 bases:

>100_bases
GGCTCGATTAATAATAGATAGAAGCCAGCGCAAGCTGGCTTTTTTGTGTTCAGTGTCTTGTCCTGAAATGTGTTTACACA
TTTAGGACTTTATAATGACT

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEHMGHVPMNHGVDFHPGLANNVAKASAMMGLTAEMLGKMHGI
TREQQDAFAVRSHQRAHAATVEGRFANEIVAIEGHDADGALIKVEHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVDDMDVIELNEAFAAQSLPCVK
DLGLMDVVEDKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP

Sequences:

>Translated_387_residues
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEHMGHVPMNHGVDFHPGLANNVAKASAMMGLTAEMLGKMHGI
TREQQDAFAVRSHQRAHAATVEGRFANEIVAIEGHDADGALIKVEHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVDDMDVIELNEAFAAQSLPCVK
DLGLMDVVEDKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP
>Mature_387_residues
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEHMGHVPMNHGVDFHPGLANNVAKASAMMGLTAEMLGKMHGI
TREQQDAFAVRSHQRAHAATVEGRFANEIVAIEGHDADGALIKVEHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVDDMDVIELNEAFAAQSLPCVK
DLGLMDVVEDKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=37.9396984924623, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=40.3553299492386, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=37.7833753148615, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4504327, Length=432, Percent_Identity=32.8703703703704, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI4557237, Length=399, Percent_Identity=33.5839598997494, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI194353979, Length=94, Percent_Identity=52.1276595744681, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI48994986, Length=386, Percent_Identity=72.279792746114, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=47.4074074074074, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.3940149625935, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=44.5, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=33.5766423357664, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=37.75, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=35.3383458646617, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=433, Percent_Identity=32.7944572748268, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=396, Percent_Identity=32.8282828282828, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17535917, Length=382, Percent_Identity=29.3193717277487, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=395, Percent_Identity=38.7341772151899, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6325229, Length=404, Percent_Identity=34.4059405940594, Blast_Score=192, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=38, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI17648125, Length=334, Percent_Identity=39.8203592814371, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=34.1860465116279, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24640423, Length=396, Percent_Identity=32.8282828282828, Blast_Score=177, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40832; Mature: 40832

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQ
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TLEQGFNIARNAALLAGIPKQVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECHHH
MGHVPMNHGVDFHPGLANNVAKASAMMGLTAEMLGKMHGITREQQDAFAVRSHQRAHAAT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHE
VEGRFANEIVAIEGHDADGALIKVEHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
ECCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCHH
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVD
HCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCC
DMDVIELNEAFAAQSLPCVKDLGLMDVVEDKVNLNGGAIALGHPLGCSGTRISTTLINLM
CCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
EAKDAKYGLATMCIGLGQGIATIFERP
HHCCCCHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQ
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TLEQGFNIARNAALLAGIPKQVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECHHH
MGHVPMNHGVDFHPGLANNVAKASAMMGLTAEMLGKMHGITREQQDAFAVRSHQRAHAAT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHE
VEGRFANEIVAIEGHDADGALIKVEHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
ECCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCHH
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVD
HCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCC
DMDVIELNEAFAAQSLPCVKDLGLMDVVEDKVNLNGGAIALGHPLGCSGTRISTTLINLM
CCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
EAKDAKYGLATMCIGLGQGIATIFERP
HHCCCCHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA