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The map label for this gene is lipB [H]

Identifier: 157826356

GI number: 157826356

Start: 1208961

End: 1209725

Strand: Reverse

Name: lipB [H]

Synonym: A1C_06725

Alternate gene names: 157826356

Gene position: 1209725-1208961 (Counterclockwise)

Preceding gene: 157826359

Following gene: 157826355

Centisome position: 98.27

GC content: 31.9

Gene sequence:

>765_bases
ATGTTACAAGTCGTAGTACTCGGGTGTGGAGCATCTATAGGCGTACCGGTGATCGGGTGTGATTGCAGTATTTGTGCATC
TCATTCAAAGTATAATAAAAGGACTAGATCAGCAATATATATTAACGATGAAAATAGTCAAATATTAGTTGATTTCGGTT
TTGATATTAGAAATCAACTATTACGAGAAAAGATCAATAAGCTTGATTGTGCTATATTAACACATTATCATTCCGATCAC
GTTAACGGTATAGATGACTTACGGGTGTTTACTTTTATGCAAGGTAAGCCATTTAAAATCTATACAGATCACGATAGTAC
GGCAAATCTCTATACAAAATTTGATTATCTATTTACTAATAAGTTATTTAAGCTTGGGCGACTGTTCACAACGCAATCTG
TTAGTTTTTTTGATAAAATTAAGATTAATACTATCGAAATACAATTTTTTAGACAACATCATGGTCCTATAGATAGTTTG
GGCTTGCGTATAGGTGATTTTGTTTATTCGCCCGATGTAATCGATTTTCCGCCTGAATCGGAAAAATTTTTAAAAGATAT
AAAAATATGGATATTAGATTGTATGGATTATAAATCAAATCTGAATCATGCAGGGCTTGATAAAGTTCTAGAATGGCGTG
AAAAATATCAACCTGAGCAGATATTATTAACTAATATGAGACATACAATAGATTATCATGAAATTACGAAAATGCTTCCA
AATAACGTTAAACCGCTTTATGACGGTTATAGATTTACGGTTTAG

Upstream 100 bases:

>100_bases
GGTATAGAGCCGATATTAGTAATTTTATTCAAAAATAAGAATATTTCTTGATTTAAGCCGTAAAAATTATAAAAAACTTC
AAACATCATTTTAAATATTT

Downstream 100 bases:

>100_bases
TTTATGATTATTTTAGAAAAAGTTTGTAAGCGTTACGGCAAGAATTATGCAATTAAGAATATTAATTTAAGTTTTACTAC
CAAGGAGACTACTGTAATAA

Product: beta-lactamase superfamily hydrolase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLQVVVLGCGASIGVPVIGCDCSICASHSKYNKRTRSAIYINDENSQILVDFGFDIRNQLLREKINKLDCAILTHYHSDH
VNGIDDLRVFTFMQGKPFKIYTDHDSTANLYTKFDYLFTNKLFKLGRLFTTQSVSFFDKIKINTIEIQFFRQHHGPIDSL
GLRIGDFVYSPDVIDFPPESEKFLKDIKIWILDCMDYKSNLNHAGLDKVLEWREKYQPEQILLTNMRHTIDYHEITKMLP
NNVKPLYDGYRFTV

Sequences:

>Translated_254_residues
MLQVVVLGCGASIGVPVIGCDCSICASHSKYNKRTRSAIYINDENSQILVDFGFDIRNQLLREKINKLDCAILTHYHSDH
VNGIDDLRVFTFMQGKPFKIYTDHDSTANLYTKFDYLFTNKLFKLGRLFTTQSVSFFDKIKINTIEIQFFRQHHGPIDSL
GLRIGDFVYSPDVIDFPPESEKFLKDIKIWILDCMDYKSNLNHAGLDKVLEWREKYQPEQILLTNMRHTIDYHEITKMLP
NNVKPLYDGYRFTV
>Mature_254_residues
MLQVVVLGCGASIGVPVIGCDCSICASHSKYNKRTRSAIYINDENSQILVDFGFDIRNQLLREKINKLDCAILTHYHSDH
VNGIDDLRVFTFMQGKPFKIYTDHDSTANLYTKFDYLFTNKLFKLGRLFTTQSVSFFDKIKINTIEIQFFRQHHGPIDSL
GLRIGDFVYSPDVIDFPPESEKFLKDIKIWILDCMDYKSNLNHAGLDKVLEWREKYQPEQILLTNMRHTIDYHEITKMLP
NNVKPLYDGYRFTV

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=253, Percent_Identity=23.3201581027668, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 29591; Mature: 29591

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQVVVLGCGASIGVPVIGCDCSICASHSKYNKRTRSAIYINDENSQILVDFGFDIRNQL
CEEEEEEECCCCCCCCEEECCCHHHCCCHHCCCCCCEEEEEECCCCEEEEEECCHHHHHH
LREKINKLDCAILTHYHSDHVNGIDDLRVFTFMQGKPFKIYTDHDSTANLYTKFDYLFTN
HHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHH
KLFKLGRLFTTQSVSFFDKIKINTIEIQFFRQHHGPIDSLGLRIGDFVYSPDVIDFPPES
HHHHHHHHHHHCCCCHHHEEEEEEEEEEEEEHHCCCHHHHCCCHHHEEECCCCCCCCCCH
EKFLKDIKIWILDCMDYKSNLNHAGLDKVLEWREKYQPEQILLTNMRHTIDYHEITKMLP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCC
NNVKPLYDGYRFTV
CCCCCHHCCEEECC
>Mature Secondary Structure
MLQVVVLGCGASIGVPVIGCDCSICASHSKYNKRTRSAIYINDENSQILVDFGFDIRNQL
CEEEEEEECCCCCCCCEEECCCHHHCCCHHCCCCCCEEEEEECCCCEEEEEECCHHHHHH
LREKINKLDCAILTHYHSDHVNGIDDLRVFTFMQGKPFKIYTDHDSTANLYTKFDYLFTN
HHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHH
KLFKLGRLFTTQSVSFFDKIKINTIEIQFFRQHHGPIDSLGLRIGDFVYSPDVIDFPPES
HHHHHHHHHHHCCCCHHHEEEEEEEEEEEEEHHCCCHHHHCCCHHHEEECCCCCCCCCCH
EKFLKDIKIWILDCMDYKSNLNHAGLDKVLEWREKYQPEQILLTNMRHTIDYHEITKMLP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCC
NNVKPLYDGYRFTV
CCCCCHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]