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The map label for this gene is dnaG [H]

Identifier: 157826345

GI number: 157826345

Start: 1198179

End: 1199966

Strand: Reverse

Name: dnaG [H]

Synonym: A1C_06670

Alternate gene names: 157826345

Gene position: 1199966-1198179 (Counterclockwise)

Preceding gene: 157826346

Following gene: 157826344

Centisome position: 97.47

GC content: 29.7

Gene sequence:

>1788_bases
ATGAGAGTTGCACCGGAATTTTATGAGTTCTTAAGAAACCGTATTAACATATTTGATGTAGTGCGGCAAAAAGTAGTTTT
AACCAGAAAATCCGGTAACTATGTCGGTTTATGTCCTTTCCATCAAGAAAAGACCCCATCTTTTACAGTTAGTGATAGTA
AGAGATTTTTCTATTGTTTTGGCTGTAAAGCAGCTGGTGACGTTATAAAATTTACTTCTAATATTAGCGGTCTATCCTAT
AATGAATCTGCTATAAAGCTTGCAACCGATTACGGTATAGAGATACCGAAATTAACTACGAAACAGAAAGAATTTTATGA
AGAATCCGATGAAATTTTAAATATTTTAGAGCTTGCTAATAAATTTTTTATAAAGCAATTAACGCCTGAAATATTAAATT
ATCTACACCAAAGAAATATTACCGAAACGACAATAAAAGAATTCTCAATAGGTTTTGCTCCAAGAAATAATAAATTTGAA
AAATTTTTTCGTGATAAAAATATAGATATTATAAAACTCGGCAAAGCCGGCTTAATAGGTAAACGAGAAAACGGTGAAAT
ATATAATTTATTTTCTAACCGTATTACTATTCCAATTAGAAATATTTACAATAAAATTGTAGGCTTTGGCGGTAGGGTAC
TTGGGGAAGGGCTACCTAAATACTTAAATTCACCTGAAACTACAGTATTTCAAAAAAGTGAAACTCTATACGGTGAGCAT
AAAGCGATAAGCAGTAGCTATAAAAAAAATCATTCGATTTTAGTAGAAGGTTATTTTGATGTTATAGCACTACATCAAGC
AGGCTTTAGTGAAGCGGTTGCGAGTCTCGGTACTAGCGTTACCGAGAATCACTTACATAAATTATGGCGTGCCGGCGATG
AAATTATATTATGTCTTGATGGAGATAATGCAGGCATAAAAGCTAGCATAAGAACTATTAACTTAGCATTACCGCTTATT
AATAGTGAAAAGAAAATTTCTTTTATTAGGTTACCAAGAGGACTTGATCCAGACGATGCAGTAAATAAACATGGTGCTGA
TTTTGTTGCAAAGCTTATAGATCAAAGAATAAGTCTATCAGAAATGATTTGGCATATTGAATATAGCGGCAAGAATTTTA
AAACCGCAGAAGAAAAAGCTAATTTAGAGAAAAATTTAAAAGATTACTGCAATAAAATATCAGATAGTAATTTAAAAGCA
AGTTATTACAGGTTTTTTAAAGATCAAATATGGCAAAATTTAGTCACAAAGCAGAAGAAAATTATAACTAAAAGCTCAAA
TTTAGCGCCTATAGTTTCAAGTCACGGCTATTCGGAGCTTGAAATACTAGAACACGCTTTTTGTGCTTTGTTGGTTAAAT
TTCCTATAATGCTTGAAGAAAAAGATATTAGAGATTTCATCTTAAATTTAAATTTTAATAATAAATCGCTTGAAGAATTT
CGTAACTGGTATTTAAACGAGATTATAGATAATAATGTAGAAGCAAGTGAAATTACTGCAATTGTAGAAAAAACTAGCTT
TTTTGATATTTTTTTATTATTATCAAAAACGGATAATTTGTTTTTAGATATCTCATTTAATAAAAATAATATCAGACTGG
ATTTATTATGGCAGTGGCTACATAAGAAATATTATCTAATAAATTTACAACAAGAATACGCTATTACCATAAACAGTACA
GACAATCACGACTTTGAAAAAGTTTTAGTATATAAAAAAGAAATTCTGAAGATTGCAAATGAACTTCAAGTTTTAAACGA
ATCTTTTATTAATCACACGATAAATTAA

Upstream 100 bases:

>100_bases
ACTGAATTTTATTCAAAAGATTTAAGAATTAACGATAATTTGTCTATACCACTTTCTTATTGTTTTTCTACAGCTATGCT
TTCCTACATATTGTAATATA

Downstream 100 bases:

>100_bases
TTATTTCAAACTTCTTGTAAAAGAAAAAGCATATAGGTATTTAATGAGCAATAGCAATATAGACAATGATCTAGATCAAA
TAGATAGTTTACTTAAAAAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MRVAPEFYEFLRNRINIFDVVRQKVVLTRKSGNYVGLCPFHQEKTPSFTVSDSKRFFYCFGCKAAGDVIKFTSNISGLSY
NESAIKLATDYGIEIPKLTTKQKEFYEESDEILNILELANKFFIKQLTPEILNYLHQRNITETTIKEFSIGFAPRNNKFE
KFFRDKNIDIIKLGKAGLIGKRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
KAISSSYKKNHSILVEGYFDVIALHQAGFSEAVASLGTSVTENHLHKLWRAGDEIILCLDGDNAGIKASIRTINLALPLI
NSEKKISFIRLPRGLDPDDAVNKHGADFVAKLIDQRISLSEMIWHIEYSGKNFKTAEEKANLEKNLKDYCNKISDSNLKA
SYYRFFKDQIWQNLVTKQKKIITKSSNLAPIVSSHGYSELEILEHAFCALLVKFPIMLEEKDIRDFILNLNFNNKSLEEF
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWLHKKYYLINLQQEYAITINST
DNHDFEKVLVYKKEILKIANELQVLNESFINHTIN

Sequences:

>Translated_595_residues
MRVAPEFYEFLRNRINIFDVVRQKVVLTRKSGNYVGLCPFHQEKTPSFTVSDSKRFFYCFGCKAAGDVIKFTSNISGLSY
NESAIKLATDYGIEIPKLTTKQKEFYEESDEILNILELANKFFIKQLTPEILNYLHQRNITETTIKEFSIGFAPRNNKFE
KFFRDKNIDIIKLGKAGLIGKRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
KAISSSYKKNHSILVEGYFDVIALHQAGFSEAVASLGTSVTENHLHKLWRAGDEIILCLDGDNAGIKASIRTINLALPLI
NSEKKISFIRLPRGLDPDDAVNKHGADFVAKLIDQRISLSEMIWHIEYSGKNFKTAEEKANLEKNLKDYCNKISDSNLKA
SYYRFFKDQIWQNLVTKQKKIITKSSNLAPIVSSHGYSELEILEHAFCALLVKFPIMLEEKDIRDFILNLNFNNKSLEEF
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWLHKKYYLINLQQEYAITINST
DNHDFEKVLVYKKEILKIANELQVLNESFINHTIN
>Mature_595_residues
MRVAPEFYEFLRNRINIFDVVRQKVVLTRKSGNYVGLCPFHQEKTPSFTVSDSKRFFYCFGCKAAGDVIKFTSNISGLSY
NESAIKLATDYGIEIPKLTTKQKEFYEESDEILNILELANKFFIKQLTPEILNYLHQRNITETTIKEFSIGFAPRNNKFE
KFFRDKNIDIIKLGKAGLIGKRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
KAISSSYKKNHSILVEGYFDVIALHQAGFSEAVASLGTSVTENHLHKLWRAGDEIILCLDGDNAGIKASIRTINLALPLI
NSEKKISFIRLPRGLDPDDAVNKHGADFVAKLIDQRISLSEMIWHIEYSGKNFKTAEEKANLEKNLKDYCNKISDSNLKA
SYYRFFKDQIWQNLVTKQKKIITKSSNLAPIVSSHGYSELEILEHAFCALLVKFPIMLEEKDIRDFILNLNFNNKSLEEF
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWLHKKYYLINLQQEYAITINST
DNHDFEKVLVYKKEILKIANELQVLNESFINHTIN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=388, Percent_Identity=35.8247422680412, Blast_Score=258, Evalue=7e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68678; Mature: 68678

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAPEFYEFLRNRINIFDVVRQKVVLTRKSGNYVGLCPFHQEKTPSFTVSDSKRFFYCF
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCEEEECCCEEEEEE
GCKAAGDVIKFTSNISGLSYNESAIKLATDYGIEIPKLTTKQKEFYEESDEILNILELAN
CCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KFFIKQLTPEILNYLHQRNITETTIKEFSIGFAPRNNKFEKFFRDKNIDIIKLGKAGLIG
HHHHHHHCHHHHHHHHHCCCHHHHHHHHEECCCCCCCHHHHHHCCCCCCEEEECCCCCCC
KRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
CCCCCCEEEEECCEEEEEHHHHHHHHHCCCCHHHHCCCHHHHCCCCCEEEECCCHHCCHH
KAISSSYKKNHSILVEGYFDVIALHQAGFSEAVASLGTSVTENHLHKLWRAGDEIILCLD
HHHHHHHCCCCCEEEEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEC
GDNAGIKASIRTINLALPLINSEKKISFIRLPRGLDPDDAVNKHGADFVAKLIDQRISLS
CCCCCEEEEEEEEEEEEEECCCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHCCHH
EMIWHIEYSGKNFKTAEEKANLEKNLKDYCNKISDSNLKASYYRFFKDQIWQNLVTKQKK
HEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IITKSSNLAPIVSSHGYSELEILEHAFCALLVKFPIMLEEKDIRDFILNLNFNNKSLEEF
HHCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEEECCCCCHHHHH
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWL
HHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCEEHHHHHHHH
HKKYYLINLQQEYAITINSTDNHDFEKVLVYKKEILKIANELQVLNESFINHTIN
CCEEEEEEEECEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVAPEFYEFLRNRINIFDVVRQKVVLTRKSGNYVGLCPFHQEKTPSFTVSDSKRFFYCF
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCEEEECCCEEEEEE
GCKAAGDVIKFTSNISGLSYNESAIKLATDYGIEIPKLTTKQKEFYEESDEILNILELAN
CCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KFFIKQLTPEILNYLHQRNITETTIKEFSIGFAPRNNKFEKFFRDKNIDIIKLGKAGLIG
HHHHHHHCHHHHHHHHHCCCHHHHHHHHEECCCCCCCHHHHHHCCCCCCEEEECCCCCCC
KRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
CCCCCCEEEEECCEEEEEHHHHHHHHHCCCCHHHHCCCHHHHCCCCCEEEECCCHHCCHH
KAISSSYKKNHSILVEGYFDVIALHQAGFSEAVASLGTSVTENHLHKLWRAGDEIILCLD
HHHHHHHCCCCCEEEEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEC
GDNAGIKASIRTINLALPLINSEKKISFIRLPRGLDPDDAVNKHGADFVAKLIDQRISLS
CCCCCEEEEEEEEEEEEEECCCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHCCHH
EMIWHIEYSGKNFKTAEEKANLEKNLKDYCNKISDSNLKASYYRFFKDQIWQNLVTKQKK
HEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IITKSSNLAPIVSSHGYSELEILEHAFCALLVKFPIMLEEKDIRDFILNLNFNNKSLEEF
HHCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEEECCCCCHHHHH
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWL
HHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCEEHHHHHHHH
HKKYYLINLQQEYAITINSTDNHDFEKVLVYKKEILKIANELQVLNESFINHTIN
CCEEEEEEEECEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]