Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 157826325

GI number: 157826325

Start: 1178414

End: 1179982

Strand: Direct

Name: hemK [H]

Synonym: A1C_06570

Alternate gene names: 157826325

Gene position: 1178414-1179982 (Clockwise)

Preceding gene: 157826324

Following gene: 157826336

Centisome position: 95.72

GC content: 32.89

Gene sequence:

>1569_bases
GTGCAATATTCTATAAAACAAGCCTTGAGTACAGCATCAGATAAATTAAATAAAATAGGTATCAGTCCGCCGCAATTAGA
AGCACGAATTTTACTTCAGCATGTCATACATAAACCTATTGAGTATTTGCTTATTAATCTTGATGAACAGTTAAGTGAAG
CTGAGATAAAAGCTTTTGAAAAATTGTTAGAGAGAAGACTAAAGCATGAACCTATAACATATATTACAGGCGTTAAAGAA
TTCTACTCACGTGAATTTATTGTCAATAAACATGTGTTAATTCCTAGAAGTGATACTGAAATTTTGGTGGATGTTGTATT
GCAGCGTTATTCCCATAGCACTCAAGGTCATTCTACCGAAAGCGGGAAACCAGAAACAAAGAAGCTGGATTCTGTGGTCA
ACCCACGGAACGACAGCATACTAGAACTCGGTACAGGTAGCGGCTGCGTTGCTATCAGTCTATTATGTGAGTTACCGAAT
GCTAATATAATTGCAACCGATATAAGCATTGAAGCAATAGAAGTTGCTAAAAGTAATGCCATAAAACATCATGTAACGGA
TCGCATTCAGATTATTCACAGTGATTGGTTTGAAAATTTAGGAAAGCAAAAATTTGATGTTATAGTTAGCAACCCACCAT
ATATATCACACAGCGAAAAATCAGAGATGGCACTTGAGACACTGAACCACGAACCGCATTTTGCATTATTTGCTGAAGAG
GACGGCTTGCAAGCTTATCGTATAATTGCTAAAAATGCCAAACCATTCTTAAAACCAAACGGTAAGATTATATTAGAGAT
AGGGTTTAACCAAGCAGAAAAAGTTAGCAAAATCTTTTTGAATTACGGCTATAATATCGACAATATTTATAAAGATTTGC
AGTCACATAATAGAGTAATAGAAATATCGTCTATTACTCTCAATCGGTCATACGCAAGGCGAGTCGGCAAAAGCTTATCA
GGAGTACAACAGAATTTATTAGATAACGAACTACTAAAATATTTATTTTCCAAAGCAAAACTTGAAAGCGAAAAGCGTAA
AGTATTTCTTGAGATAGGTTTTGGTATGGGTGAGCATTTCATTAACCAAGCAAAAATGAATCCTGATACACTTTTTATTG
GTGTAGAGGTATATTTAAACGGAGTTGCAAATGTTTTAAAACTTGCTGCTGAGCAGAATATCAAGAATTTTTTATTATTT
CCGAATAATTTGGACTTAATATTAAACGATTTACCAAATAATAGTTTAGATGGAATTTATATTTTATTTCCTGATCCATG
GATAAAAAATAAGCAAAAAAAGAAACGTATTTTCAATAAAGAGCGTCTTAAGGTTTTACAAGATAAGCTAAAAGATAACG
GTAATTTAGTATTTGCTTCTGATATCGAAAATTATTTTTATGAAGCAATTGAACTAATAAAACAGCACGGTAATTTTGAA
ATTATGAATAACGATGATTATCTAACCTCACATGATAATTATGTAATGACCAAATATCATCAAAAAGCTATTAAGGAGAA
TAGAACGCCGAAATTTATGATTTTGCGGCACGTTTTAGGCGATCATTAA

Upstream 100 bases:

>100_bases
AACGCTTAATTTTGTGGCATATTATAGCTACTGTTAGCCCCATGATTATGAAAACAATATATAAAGCAATATTAGAAATT
ATAGGAGTTGGACCTGTTCC

Downstream 100 bases:

>100_bases
TTGCAGCACCAATATTTTCTTGAGGTATAGGCGCAACTGCTATACCGCTTACATCATGAGCTGCAGCATAGTCGTCAAGA
ATTCTTAAATACGCATAAAG

Product: bifunctional N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase/tRNA (m7G46) methyltransferase

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MQYSIKQALSTASDKLNKIGISPPQLEARILLQHVIHKPIEYLLINLDEQLSEAEIKAFEKLLERRLKHEPITYITGVKE
FYSREFIVNKHVLIPRSDTEILVDVVLQRYSHSTQGHSTESGKPETKKLDSVVNPRNDSILELGTGSGCVAISLLCELPN
ANIIATDISIEAIEVAKSNAIKHHVTDRIQIIHSDWFENLGKQKFDVIVSNPPYISHSEKSEMALETLNHEPHFALFAEE
DGLQAYRIIAKNAKPFLKPNGKIILEIGFNQAEKVSKIFLNYGYNIDNIYKDLQSHNRVIEISSITLNRSYARRVGKSLS
GVQQNLLDNELLKYLFSKAKLESEKRKVFLEIGFGMGEHFINQAKMNPDTLFIGVEVYLNGVANVLKLAAEQNIKNFLLF
PNNLDLILNDLPNNSLDGIYILFPDPWIKNKQKKKRIFNKERLKVLQDKLKDNGNLVFASDIENYFYEAIELIKQHGNFE
IMNNDDYLTSHDNYVMTKYHQKAIKENRTPKFMILRHVLGDH

Sequences:

>Translated_522_residues
MQYSIKQALSTASDKLNKIGISPPQLEARILLQHVIHKPIEYLLINLDEQLSEAEIKAFEKLLERRLKHEPITYITGVKE
FYSREFIVNKHVLIPRSDTEILVDVVLQRYSHSTQGHSTESGKPETKKLDSVVNPRNDSILELGTGSGCVAISLLCELPN
ANIIATDISIEAIEVAKSNAIKHHVTDRIQIIHSDWFENLGKQKFDVIVSNPPYISHSEKSEMALETLNHEPHFALFAEE
DGLQAYRIIAKNAKPFLKPNGKIILEIGFNQAEKVSKIFLNYGYNIDNIYKDLQSHNRVIEISSITLNRSYARRVGKSLS
GVQQNLLDNELLKYLFSKAKLESEKRKVFLEIGFGMGEHFINQAKMNPDTLFIGVEVYLNGVANVLKLAAEQNIKNFLLF
PNNLDLILNDLPNNSLDGIYILFPDPWIKNKQKKKRIFNKERLKVLQDKLKDNGNLVFASDIENYFYEAIELIKQHGNFE
IMNNDDYLTSHDNYVMTKYHQKAIKENRTPKFMILRHVLGDH
>Mature_522_residues
MQYSIKQALSTASDKLNKIGISPPQLEARILLQHVIHKPIEYLLINLDEQLSEAEIKAFEKLLERRLKHEPITYITGVKE
FYSREFIVNKHVLIPRSDTEILVDVVLQRYSHSTQGHSTESGKPETKKLDSVVNPRNDSILELGTGSGCVAISLLCELPN
ANIIATDISIEAIEVAKSNAIKHHVTDRIQIIHSDWFENLGKQKFDVIVSNPPYISHSEKSEMALETLNHEPHFALFAEE
DGLQAYRIIAKNAKPFLKPNGKIILEIGFNQAEKVSKIFLNYGYNIDNIYKDLQSHNRVIEISSITLNRSYARRVGKSLS
GVQQNLLDNELLKYLFSKAKLESEKRKVFLEIGFGMGEHFINQAKMNPDTLFIGVEVYLNGVANVLKLAAEQNIKNFLLF
PNNLDLILNDLPNNSLDGIYILFPDPWIKNKQKKKRIFNKERLKVLQDKLKDNGNLVFASDIENYFYEAIELIKQHGNFE
IMNNDDYLTSHDNYVMTKYHQKAIKENRTPKFMILRHVLGDH

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=262, Percent_Identity=30.5343511450382, Blast_Score=106, Evalue=7e-23,
Organism=Escherichia coli, GI1787463, Length=288, Percent_Identity=34.375, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI87082085, Length=236, Percent_Identity=31.3559322033898, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789330, Length=204, Percent_Identity=29.9019607843137, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24582226, Length=302, Percent_Identity=26.4900662251656, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 59847; Mature: 59847

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYSIKQALSTASDKLNKIGISPPQLEARILLQHVIHKPIEYLLINLDEQLSEAEIKAFE
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHH
KLLERRLKHEPITYITGVKEFYSREFIVNKHVLIPRSDTEILVDVVLQRYSHSTQGHSTE
HHHHHHHCCCCCHHHHHHHHHHCCCEEEECEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
SGKPETKKLDSVVNPRNDSILELGTGSGCVAISLLCELPNANIIATDISIEAIEVAKSNA
CCCCHHHHHHHHCCCCCCCEEEECCCCCCEEEHHHHCCCCCCEEEEECEEEEHHHHHHCC
IKHHVTDRIQIIHSDWFENLGKQKFDVIVSNPPYISHSEKSEMALETLNHEPHFALFAEE
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECC
DGLQAYRIIAKNAKPFLKPNGKIILEIGFNQAEKVSKIFLNYGYNIDNIYKDLQSHNRVI
CCCCEEHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEE
EISSITLNRSYARRVGKSLSGVQQNLLDNELLKYLFSKAKLESEKRKVFLEIGFGMGEHF
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
INQAKMNPDTLFIGVEVYLNGVANVLKLAAEQNIKNFLLFPNNLDLILNDLPNNSLDGIY
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCEEE
ILFPDPWIKNKQKKKRIFNKERLKVLQDKLKDNGNLVFASDIENYFYEAIELIKQHGNFE
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEE
IMNNDDYLTSHDNYVMTKYHQKAIKENRTPKFMILRHVLGDH
EECCCCCEECCCCEEEHHHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQYSIKQALSTASDKLNKIGISPPQLEARILLQHVIHKPIEYLLINLDEQLSEAEIKAFE
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHH
KLLERRLKHEPITYITGVKEFYSREFIVNKHVLIPRSDTEILVDVVLQRYSHSTQGHSTE
HHHHHHHCCCCCHHHHHHHHHHCCCEEEECEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
SGKPETKKLDSVVNPRNDSILELGTGSGCVAISLLCELPNANIIATDISIEAIEVAKSNA
CCCCHHHHHHHHCCCCCCCEEEECCCCCCEEEHHHHCCCCCCEEEEECEEEEHHHHHHCC
IKHHVTDRIQIIHSDWFENLGKQKFDVIVSNPPYISHSEKSEMALETLNHEPHFALFAEE
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECC
DGLQAYRIIAKNAKPFLKPNGKIILEIGFNQAEKVSKIFLNYGYNIDNIYKDLQSHNRVI
CCCCEEHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEE
EISSITLNRSYARRVGKSLSGVQQNLLDNELLKYLFSKAKLESEKRKVFLEIGFGMGEHF
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
INQAKMNPDTLFIGVEVYLNGVANVLKLAAEQNIKNFLLFPNNLDLILNDLPNNSLDGIY
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCEEE
ILFPDPWIKNKQKKKRIFNKERLKVLQDKLKDNGNLVFASDIENYFYEAIELIKQHGNFE
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEE
IMNNDDYLTSHDNYVMTKYHQKAIKENRTPKFMILRHVLGDH
EECCCCCEECCCCEEEHHHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]