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The map label for this gene is proP [H]

Identifier: 157826307

GI number: 157826307

Start: 1161937

End: 1162494

Strand: Direct

Name: proP [H]

Synonym: A1C_06480

Alternate gene names: 157826307

Gene position: 1161937-1162494 (Clockwise)

Preceding gene: 157826306

Following gene: 157826308

Centisome position: 94.39

GC content: 33.87

Gene sequence:

>558_bases
ATGAGCGTTTTTCCAAAAGCCGATGCTTATGCTATTTCGCTTTACTCTATAGCAGTTTTATGTTCTACTTTTATCTTGAG
AGATCTCGTTTTCGGATATTTAGGCGATACATATGGACGCAAAACTACAGTAGTAATAACAATTCTGATGATGGATACTT
CATGCATAGTCATGGCATTACTTCCTACTTACAATGCAATTGGTATAACAGCTTCTATTATAATAATATTATGTAGAATT
GTTCAAGGTATGTATGCAATTGGAGAGGTAATAGGAACAGAAATTTATTTAACTGAGTTTACTAAACCTCCAATACAATA
TCCTATTGTAGCCTTAGCTACGGTTTTCGCTACTGTAGGAACTTTCGCAGCTTTAGGGGTTGCTACTATTGTTACTTCTT
ATTGCTTTAATTGGCGTTATGCATTTGGTATCGGTGCAATTATTGCCGTTGTAAGAACATTAGTAAGAACCGCATTACGA
GAAACTCCTGAATTTGCTAAAATGCAAAGCTTAAATTACTTAAAACTGTTTTTATTTTGCATATATTTATTGCGGTGA

Upstream 100 bases:

>100_bases
TTGATAAAAGAACAGAAACAAGCTGTAGGGTTGTGATCTGTAGGAACCTTATTGGAATATTTTGATCTTATGCTTTATGT
CCATATGTCGGTCCTTCTTA

Downstream 100 bases:

>100_bases
TATTCTTATAACCAAATTTAACTGTACTCCTGAACAAATTATAAAACAAAATTTCAAATTATCTATGATAGAACTTATAA
GCATAATAATAGTATGCAGC

Product: MFS type sugar transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSVFPKADAYAISLYSIAVLCSTFILRDLVFGYLGDTYGRKTTVVITILMMDTSCIVMALLPTYNAIGITASIIIILCRI
VQGMYAIGEVIGTEIYLTEFTKPPIQYPIVALATVFATVGTFAALGVATIVTSYCFNWRYAFGIGAIIAVVRTLVRTALR
ETPEFAKMQSLNYLKLFLFCIYLLR

Sequences:

>Translated_185_residues
MSVFPKADAYAISLYSIAVLCSTFILRDLVFGYLGDTYGRKTTVVITILMMDTSCIVMALLPTYNAIGITASIIIILCRI
VQGMYAIGEVIGTEIYLTEFTKPPIQYPIVALATVFATVGTFAALGVATIVTSYCFNWRYAFGIGAIIAVVRTLVRTALR
ETPEFAKMQSLNYLKLFLFCIYLLR
>Mature_184_residues
SVFPKADAYAISLYSIAVLCSTFILRDLVFGYLGDTYGRKTTVVITILMMDTSCIVMALLPTYNAIGITASIIIILCRIV
QGMYAIGEVIGTEIYLTEFTKPPIQYPIVALATVFATVGTFAALGVATIVTSYCFNWRYAFGIGAIIAVVRTLVRTALRE
TPEFAKMQSLNYLKLFLFCIYLLR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=176, Percent_Identity=32.3863636363636, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789941, Length=172, Percent_Identity=33.7209302325581, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788292, Length=178, Percent_Identity=24.7191011235955, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 20438; Mature: 20307

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFPKADAYAISLYSIAVLCSTFILRDLVFGYLGDTYGRKTTVVITILMMDTSCIVMAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LPTYNAIGITASIIIILCRIVQGMYAIGEVIGTEIYLTEFTKPPIQYPIVALATVFATVG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHHHHHHHHHHH
TFAALGVATIVTSYCFNWRYAFGIGAIIAVVRTLVRTALRETPEFAKMQSLNYLKLFLFC
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IYLLR
HHHHC
>Mature Secondary Structure 
SVFPKADAYAISLYSIAVLCSTFILRDLVFGYLGDTYGRKTTVVITILMMDTSCIVMAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LPTYNAIGITASIIIILCRIVQGMYAIGEVIGTEIYLTEFTKPPIQYPIVALATVFATVG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHHHHHHHHHHH
TFAALGVATIVTSYCFNWRYAFGIGAIIAVVRTLVRTALRETPEFAKMQSLNYLKLFLFC
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IYLLR
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]