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The map label for this gene is lipA

Identifier: 157826178

GI number: 157826178

Start: 1023624

End: 1024517

Strand: Direct

Name: lipA

Synonym: A1C_05775

Alternate gene names: 157826178

Gene position: 1023624-1024517 (Clockwise)

Preceding gene: 157826177

Following gene: 157826179

Centisome position: 83.15

GC content: 35.79

Gene sequence:

>894_bases
ATGACTAATTTAAATAAAAAACCGGATTGGATAAAGGTTAAAGCTCCTAATTCTGCAGAGTATTATAATACAAAAGATTT
AATAAAGAATCTGAGTTTAAATACTGTGTGCGAAGAAGCTGCTTGTCCTAATATCGGTGAGTGTTGGTCGAAAAAACATG
CAACCATGATTATTTTAGGTTCGGTTTGTACTAGAGCCTGTATGTTTTGTAATGTCAAAACTGGTAGACCTGATTTACTT
GATCCGCACGAACCGCAAAGGTTAGCAGAGGCTGTACAAAAGTTAAATCTTAAACATGTAGTAATTACTTCCGTCGATCG
TGATGACCTTGAAGACGGTGGAGCTTCACATTTTGCGGAGTGTATTAATGAAATTAGAAAATCCTCGACAAATACTACTA
TCGAGATTCTAACACCCGATTTTTTAAGAAAAGAAGGAGCAGCTGAAATAATAGCCAATTCAAAGCCTGACGTATTTAAT
CATAATGTCGAAACAGTACCGTCTTTATATAAAACGATTAGACCTGGAGCTAGATATTATAATTCTTTAAGCTTACTTCA
CAATATCAAAAAATTATCTCCTGAAATTTTTACAAAATCAGGTATGATGGTAGGACTTGGCGAAGAAATAAATGAAGTAG
TACAGGTTATGGATGATTTAAGGGAAGCACAAGTTGATTTCCTCACTATCGGACAGTATCTGCAACCTACTAAAAATCAT
GCCGAGGTCGCAAAATATGTTACTCCTGAAGAGTTTCAATATTTAGGGCGAATAGCAAAAACAAAAGGATTCTTGATGGT
TTCGTCAACCCCGCTAACCCGTTCTTCATACCATGCCGATGAGGATTTTCAAAAATTAAAAGAGAATTACCAACAGCGTC
ATACAGTGGCTTAA

Upstream 100 bases:

>100_bases
GCAGATATTTTAGATGGTCTAAAGAATAATAAAGATAATACTAAAGCTGAACAGCAAGTGCTACATAAAGTAACAAAACT
AATTAAATTATTTCCGTTTT

Downstream 100 bases:

>100_bases
CAGGTGTTGTTGTATGGATACTCGAACCGTCATTACGAGAAGAAGCGTAGTAGCATCGACGTGGCAATCTCATCAAGCAA
GTAGTACCAAAGTCCTGAGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTNLNKKPDWIKVKAPNSAEYYNTKDLIKNLSLNTVCEEAACPNIGECWSKKHATMIILGSVCTRACMFCNVKTGRPDLL
DPHEPQRLAEAVQKLNLKHVVITSVDRDDLEDGGASHFAECINEIRKSSTNTTIEILTPDFLRKEGAAEIIANSKPDVFN
HNVETVPSLYKTIRPGARYYNSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVMDDLREAQVDFLTIGQYLQPTKNH
AEVAKYVTPEEFQYLGRIAKTKGFLMVSSTPLTRSSYHADEDFQKLKENYQQRHTVA

Sequences:

>Translated_297_residues
MTNLNKKPDWIKVKAPNSAEYYNTKDLIKNLSLNTVCEEAACPNIGECWSKKHATMIILGSVCTRACMFCNVKTGRPDLL
DPHEPQRLAEAVQKLNLKHVVITSVDRDDLEDGGASHFAECINEIRKSSTNTTIEILTPDFLRKEGAAEIIANSKPDVFN
HNVETVPSLYKTIRPGARYYNSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVMDDLREAQVDFLTIGQYLQPTKNH
AEVAKYVTPEEFQYLGRIAKTKGFLMVSSTPLTRSSYHADEDFQKLKENYQQRHTVA
>Mature_296_residues
TNLNKKPDWIKVKAPNSAEYYNTKDLIKNLSLNTVCEEAACPNIGECWSKKHATMIILGSVCTRACMFCNVKTGRPDLLD
PHEPQRLAEAVQKLNLKHVVITSVDRDDLEDGGASHFAECINEIRKSSTNTTIEILTPDFLRKEGAAEIIANSKPDVFNH
NVETVPSLYKTIRPGARYYNSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVMDDLREAQVDFLTIGQYLQPTKNHA
EVAKYVTPEEFQYLGRIAKTKGFLMVSSTPLTRSSYHADEDFQKLKENYQQRHTVA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=45.9649122807018, Blast_Score=264, Evalue=6e-71,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=46.0905349794239, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1786846, Length=279, Percent_Identity=50.179211469534, Blast_Score=293, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=46.1240310077519, Blast_Score=236, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=285, Percent_Identity=47.3684210526316, Blast_Score=268, Evalue=8e-73,
Organism=Drosophila melanogaster, GI221513272, Length=295, Percent_Identity=44.7457627118644, Blast_Score=255, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_RICAH (A8GPR5)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493898.1
- ProteinModelPortal:   A8GPR5
- STRING:   A8GPR5
- GeneID:   5645821
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_05775
- NMPDR:   fig|293614.3.peg.1085
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   DPHEPQR
- ProtClustDB:   PRK05481
- BioCyc:   RAKA293614:A1C_05775-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33493; Mature: 33361

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLNKKPDWIKVKAPNSAEYYNTKDLIKNLSLNTVCEEAACPNIGECWSKKHATMIILG
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHCCCCHHHHHCCCCCEEEEHH
SVCTRACMFCNVKTGRPDLLDPHEPQRLAEAVQKLNLKHVVITSVDRDDLEDGGASHFAE
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHHHHH
CINEIRKSSTNTTIEILTPDFLRKEGAAEIIANSKPDVFNHNVETVPSLYKTIRPGARYY
HHHHHHHCCCCCEEEEECHHHHHHCCCHHHHCCCCCCHHCCCHHHHHHHHHHHCCHHHHH
NSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVMDDLREAQVDFLTIGQYLQPTKNH
HHHHHHHHHHHHCHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AEVAKYVTPEEFQYLGRIAKTKGFLMVSSTPLTRSSYHADEDFQKLKENYQQRHTVA
HHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNLNKKPDWIKVKAPNSAEYYNTKDLIKNLSLNTVCEEAACPNIGECWSKKHATMIILG
CCCCCCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHCCCCHHHHHCCCCCEEEEHH
SVCTRACMFCNVKTGRPDLLDPHEPQRLAEAVQKLNLKHVVITSVDRDDLEDGGASHFAE
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHHHHH
CINEIRKSSTNTTIEILTPDFLRKEGAAEIIANSKPDVFNHNVETVPSLYKTIRPGARYY
HHHHHHHCCCCCEEEEECHHHHHHCCCHHHHCCCCCCHHCCCHHHHHHHHHHHCCHHHHH
NSLSLLHNIKKLSPEIFTKSGMMVGLGEEINEVVQVMDDLREAQVDFLTIGQYLQPTKNH
HHHHHHHHHHHHCHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AEVAKYVTPEEFQYLGRIAKTKGFLMVSSTPLTRSSYHADEDFQKLKENYQQRHTVA
HHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA