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The map label for this gene is proP [H]

Identifier: 157826154

GI number: 157826154

Start: 1009019

End: 1010290

Strand: Direct

Name: proP [H]

Synonym: A1C_05655

Alternate gene names: 157826154

Gene position: 1009019-1010290 (Clockwise)

Preceding gene: 157826153

Following gene: 157826155

Centisome position: 81.96

GC content: 33.18

Gene sequence:

>1272_bases
ATGAAACAAATAAAACCAAGCAATAAAGTTCTCGGTGCTTTTTTAGGCACTATCATTGAATATTACGACTATAGTTTATA
TGGTTTTTCAGCGTCAATTATCGCAGATAAATTCTTTTCACCCGGTACTGATCCGTTAACAAAGCTTGTAAATGTTTTTG
CCGTTTATGCAGCAGCATATTTATCAAAACCTATAGGGGCCTATATTTTTGGACGTATAGGGGATATATACGGTCGAAAA
AAAGCCTTAAGTTTTACGATTATCGGTATTGTAATACCTACCTTAATAATAGGTTTATTGCCTGATTATTCTTCGATAGG
AGTTTGGAGTACGATAATGTTAGTTTTATGTCGCTTTATGCAAGGTATTTTTATTGGAGGGGAATATGACGGTGCGGCAA
TTTATGTTATTGAACATTTAGAAGCAAAATACCGATTTACTGCCTCTGCAATAACTAGATGTACAGGAGTAATCGGTTTA
TTATGCGGGATTGGTGCAACTAATTTCTTCAATTCTCATATTTTTCCGGAGTGGTGTTGGCGTATTCCATTTTTACTAAG
TTTACCGCTTGCATTAATAACTCTTTATTATCGCCGAAAATTTGATGAAACACCGGAGTTTAAAAAAGCACATCATAGCC
AAGATTCTATCCAAACACTTAGCTCTATAATTAAAAAGCAATGGAAAAATATTGCACGGTTGATATTTTTGGCTGGAGGT
TTTGGAGCAACATATCAAATTGCGATCATCTTTATGAAGCAATATTTACCAATCGTATTACCTTCAGTCGGTATTATTAT
GAGTTCTTTTTCAGTATTAATAGTAATATGTTTTGCTGTTTGCATGCCGATTGCCGGCTTTATTGCTGACCGCTTAGGAG
TTAATTCAGTATTAAAAATCGCATTGATATGTACTATTACAGCAAGTGTATTTTTTGTGATAGCAGTAAAATATCAAATG
ACTAATTTAGGGCTTACAGCTAGTTTAATGTTAGCTGCATCGGTTACTCCTTTTAATGCTCTTGCTCATAGTATAGTTAT
TAAGTCTTTTGTAGTTAAGGAACGTTACCGTGTTATAAGTTTAGGACATAATATCGGTTCAATGTTAATGTCTGGTACTG
CTAATTATATTTGTGCAAAGGTAATAAAATCATTGGATTTTAACCTATTCCCGATTTTATATCTTTGTATGTTTGCCGTA
CTTGCTTATTCTATGACGATGTTGTTTAGTAATGATACCATATTAAATCAAGATCTAAAAAACGACAAATAA

Upstream 100 bases:

>100_bases
CTTTACTGGATTCCTGCCTACGCGGGAATGACATCGAGTAGGTTTTTCAACACATGCAACAAAGCTGGATGACACCAAAA
AACGACAAATCACCTTTAAA

Downstream 100 bases:

>100_bases
ACATAAGTAATAAATTGTAATTATCGTAAAAAATGACATATTATAATAAATGTTAATTTAGAGTTATAATATGGAAAGAA
TAGTGACCAACATGGATCAG

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKQIKPSNKVLGAFLGTIIEYYDYSLYGFSASIIADKFFSPGTDPLTKLVNVFAVYAAAYLSKPIGAYIFGRIGDIYGRK
KALSFTIIGIVIPTLIIGLLPDYSSIGVWSTIMLVLCRFMQGIFIGGEYDGAAIYVIEHLEAKYRFTASAITRCTGVIGL
LCGIGATNFFNSHIFPEWCWRIPFLLSLPLALITLYYRRKFDETPEFKKAHHSQDSIQTLSSIIKKQWKNIARLIFLAGG
FGATYQIAIIFMKQYLPIVLPSVGIIMSSFSVLIVICFAVCMPIAGFIADRLGVNSVLKIALICTITASVFFVIAVKYQM
TNLGLTASLMLAASVTPFNALAHSIVIKSFVVKERYRVISLGHNIGSMLMSGTANYICAKVIKSLDFNLFPILYLCMFAV
LAYSMTMLFSNDTILNQDLKNDK

Sequences:

>Translated_423_residues
MKQIKPSNKVLGAFLGTIIEYYDYSLYGFSASIIADKFFSPGTDPLTKLVNVFAVYAAAYLSKPIGAYIFGRIGDIYGRK
KALSFTIIGIVIPTLIIGLLPDYSSIGVWSTIMLVLCRFMQGIFIGGEYDGAAIYVIEHLEAKYRFTASAITRCTGVIGL
LCGIGATNFFNSHIFPEWCWRIPFLLSLPLALITLYYRRKFDETPEFKKAHHSQDSIQTLSSIIKKQWKNIARLIFLAGG
FGATYQIAIIFMKQYLPIVLPSVGIIMSSFSVLIVICFAVCMPIAGFIADRLGVNSVLKIALICTITASVFFVIAVKYQM
TNLGLTASLMLAASVTPFNALAHSIVIKSFVVKERYRVISLGHNIGSMLMSGTANYICAKVIKSLDFNLFPILYLCMFAV
LAYSMTMLFSNDTILNQDLKNDK
>Mature_423_residues
MKQIKPSNKVLGAFLGTIIEYYDYSLYGFSASIIADKFFSPGTDPLTKLVNVFAVYAAAYLSKPIGAYIFGRIGDIYGRK
KALSFTIIGIVIPTLIIGLLPDYSSIGVWSTIMLVLCRFMQGIFIGGEYDGAAIYVIEHLEAKYRFTASAITRCTGVIGL
LCGIGATNFFNSHIFPEWCWRIPFLLSLPLALITLYYRRKFDETPEFKKAHHSQDSIQTLSSIIKKQWKNIARLIFLAGG
FGATYQIAIIFMKQYLPIVLPSVGIIMSSFSVLIVICFAVCMPIAGFIADRLGVNSVLKIALICTITASVFFVIAVKYQM
TNLGLTASLMLAASVTPFNALAHSIVIKSFVVKERYRVISLGHNIGSMLMSGTANYICAKVIKSLDFNLFPILYLCMFAV
LAYSMTMLFSNDTILNQDLKNDK

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=416, Percent_Identity=29.5673076923077, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=28.6697247706422, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=26.555023923445, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789941, Length=396, Percent_Identity=28.030303030303, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87082404, Length=328, Percent_Identity=25.9146341463415, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=25.5605381165919, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46840; Mature: 46840

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIKPSNKVLGAFLGTIIEYYDYSLYGFSASIIADKFFSPGTDPLTKLVNVFAVYAAAY
CCCCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LSKPIGAYIFGRIGDIYGRKKALSFTIIGIVIPTLIIGLLPDYSSIGVWSTIMLVLCRFM
HHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
QGIFIGGEYDGAAIYVIEHLEAKYRFTASAITRCTGVIGLLCGIGATNFFNSHIFPEWCW
CCEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHH
RIPFLLSLPLALITLYYRRKFDETPEFKKAHHSQDSIQTLSSIIKKQWKNIARLIFLAGG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FGATYQIAIIFMKQYLPIVLPSVGIIMSSFSVLIVICFAVCMPIAGFIADRLGVNSVLKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ALICTITASVFFVIAVKYQMTNLGLTASLMLAASVTPFNALAHSIVIKSFVVKERYRVIS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGHNIGSMLMSGTANYICAKVIKSLDFNLFPILYLCMFAVLAYSMTMLFSNDTILNQDLK
CCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NDK
CCC
>Mature Secondary Structure
MKQIKPSNKVLGAFLGTIIEYYDYSLYGFSASIIADKFFSPGTDPLTKLVNVFAVYAAAY
CCCCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LSKPIGAYIFGRIGDIYGRKKALSFTIIGIVIPTLIIGLLPDYSSIGVWSTIMLVLCRFM
HHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
QGIFIGGEYDGAAIYVIEHLEAKYRFTASAITRCTGVIGLLCGIGATNFFNSHIFPEWCW
CCEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHH
RIPFLLSLPLALITLYYRRKFDETPEFKKAHHSQDSIQTLSSIIKKQWKNIARLIFLAGG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FGATYQIAIIFMKQYLPIVLPSVGIIMSSFSVLIVICFAVCMPIAGFIADRLGVNSVLKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ALICTITASVFFVIAVKYQMTNLGLTASLMLAASVTPFNALAHSIVIKSFVVKERYRVIS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGHNIGSMLMSGTANYICAKVIKSLDFNLFPILYLCMFAVLAYSMTMLFSNDTILNQDLK
CCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NDK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]