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The map label for this gene is tgt [H]

Identifier: 157826145

GI number: 157826145

Start: 997444

End: 998529

Strand: Reverse

Name: tgt [H]

Synonym: A1C_05610

Alternate gene names: 157826145

Gene position: 998529-997444 (Counterclockwise)

Preceding gene: 157826146

Following gene: 157826144

Centisome position: 81.11

GC content: 41.71

Gene sequence:

>1086_bases
GTGTCAAAATTCTCATTTAATATTCATCATCAGCATAAAAAAGCTAGAAGCGGTATTATCGCAACTGCACACGGTGAAAT
TCGTACTCCGGCCTTTATGCCGGTCGGGACTAGAGGAACTGTTAAGGCAATGTTGCCTGAATCGGTAGCAGAAACCGGAG
CTGATATATTACTGGGTAATACTTACCACTTAATGCTTCAGCCAAGTGCCGAGCGTATAGCACGCCTTGGCGGTCTGCAT
AAATTCATGAATTGGGATAAGCCGATATTAACCGATTCCGGCGGTTTTCAGGTAATGTCGCTATCGAAGTTACGGAAGAT
AACCGAAGAAGGAGTAAGTTTCAGCTCTCATATTAACGGTGATAAATATATGTTAACGCCTGAGCGTTCTACCGCAATAC
AATATCTGCTTGGTAGCACGATCACTATGGCTTTTGACGAATGTACGCCGTACCCTGTTACATTTGAAGAAGCTAAAACT
TCTATGCAGCTTACGACTAGATGGGCGCATAGATCACGTAATGCCTTTGTTAAGCGGGAAGGCTACGCACAATTCGGTAT
TATTCAAGGTAGTGTTTACGAAGAATTACGTGAGCAATCAGCTAAAGGTTTAGTAGAACTCGATTTTGATGGCTATGCTA
TAGGCGGGCTTGCAGTAGGTGAGGGGCAGGAGCTGATGTTTAAAGTGCTTGATTATGCTCCGGATTTTCTGCCGCAAAAT
AAGCCGCGTTATTTAATGGGAGTCGGTAAGCCTGCTGATATTATCGGTGCGGTTAACCGAGGTATAGATATGTTTGACTG
CGTAATTCCGACACGCTCAGGACGTAACGGTCAGGCTTTTACAAAATACGGTACGGTAAATATCTGCAATAGCAAATACT
CAGGTGATAATGAGCCGCTAGAACATGATTGTCCATGTCCTGCTTGCACTAATTACACGAAAGCTTATCTGCATCATTTA
GTTAGAATCGGTGAGATACTTGGTTCTATGCTAATGACCTGGCATAATTTAACATATTTTCAAAACCTCATAAGCCGTAT
TAGAGAATATATTAAACTAGGTAAAGATTTTGATTTTTATTCTTAA

Upstream 100 bases:

>100_bases
ATAGAGCCGTTAGCAAAATCGCTTTAATTAGCTTTAAAATTATTTTGTTAACATTCCTATAGTATGCTAAGATATTGCTT
TTAAAAACAAGTGATTATAC

Downstream 100 bases:

>100_bases
TAAGCCCTTAGTTGACTCATTATAAAACGTACTTAGATATGTTATGTTATGGACAAGGTTTATGTAGATCATACTTCAGT
GATTGTAGATAGACAAAAAC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSKFSFNIHHQHKKARSGIIATAHGEIRTPAFMPVGTRGTVKAMLPESVAETGADILLGNTYHLMLQPSAERIARLGGLH
KFMNWDKPILTDSGGFQVMSLSKLRKITEEGVSFSSHINGDKYMLTPERSTAIQYLLGSTITMAFDECTPYPVTFEEAKT
SMQLTTRWAHRSRNAFVKREGYAQFGIIQGSVYEELREQSAKGLVELDFDGYAIGGLAVGEGQELMFKVLDYAPDFLPQN
KPRYLMGVGKPADIIGAVNRGIDMFDCVIPTRSGRNGQAFTKYGTVNICNSKYSGDNEPLEHDCPCPACTNYTKAYLHHL
VRIGEILGSMLMTWHNLTYFQNLISRIREYIKLGKDFDFYS

Sequences:

>Translated_361_residues
MSKFSFNIHHQHKKARSGIIATAHGEIRTPAFMPVGTRGTVKAMLPESVAETGADILLGNTYHLMLQPSAERIARLGGLH
KFMNWDKPILTDSGGFQVMSLSKLRKITEEGVSFSSHINGDKYMLTPERSTAIQYLLGSTITMAFDECTPYPVTFEEAKT
SMQLTTRWAHRSRNAFVKREGYAQFGIIQGSVYEELREQSAKGLVELDFDGYAIGGLAVGEGQELMFKVLDYAPDFLPQN
KPRYLMGVGKPADIIGAVNRGIDMFDCVIPTRSGRNGQAFTKYGTVNICNSKYSGDNEPLEHDCPCPACTNYTKAYLHHL
VRIGEILGSMLMTWHNLTYFQNLISRIREYIKLGKDFDFYS
>Mature_360_residues
SKFSFNIHHQHKKARSGIIATAHGEIRTPAFMPVGTRGTVKAMLPESVAETGADILLGNTYHLMLQPSAERIARLGGLHK
FMNWDKPILTDSGGFQVMSLSKLRKITEEGVSFSSHINGDKYMLTPERSTAIQYLLGSTITMAFDECTPYPVTFEEAKTS
MQLTTRWAHRSRNAFVKREGYAQFGIIQGSVYEELREQSAKGLVELDFDGYAIGGLAVGEGQELMFKVLDYAPDFLPQNK
PRYLMGVGKPADIIGAVNRGIDMFDCVIPTRSGRNGQAFTKYGTVNICNSKYSGDNEPLEHDCPCPACTNYTKAYLHHLV
RIGEILGSMLMTWHNLTYFQNLISRIREYIKLGKDFDFYS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=346, Percent_Identity=39.5953757225434, Blast_Score=269, Evalue=2e-72,
Organism=Escherichia coli, GI1786607, Length=353, Percent_Identity=55.2407932011331, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17544680, Length=339, Percent_Identity=41.8879056047198, Blast_Score=263, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI25146502, Length=163, Percent_Identity=32.5153374233129, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19920510, Length=339, Percent_Identity=43.3628318584071, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI21355311, Length=181, Percent_Identity=27.0718232044199, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 40426; Mature: 40295

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFSFNIHHQHKKARSGIIATAHGEIRTPAFMPVGTRGTVKAMLPESVAETGADILLGN
CCCEEEEHHHHHHHHHCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEECC
TYHLMLQPSAERIARLGGLHKFMNWDKPILTDSGGFQVMSLSKLRKITEEGVSFSSHING
EEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHCCCHHHCCCC
DKYMLTPERSTAIQYLLGSTITMAFDECTPYPVTFEEAKTSMQLTTRWAHRSRNAFVKRE
CEEEECCCHHHHHHHHHCCHHEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEECC
GYAQFGIIQGSVYEELREQSAKGLVELDFDGYAIGGLAVGEGQELMFKVLDYAPDFLPQN
CCCEEEEEECHHHHHHHHHHCCCEEEECCCCEEECCEEECCCHHHHHHHHHHCHHHCCCC
KPRYLMGVGKPADIIGAVNRGIDMFDCVIPTRSGRNGQAFTKYGTVNICNSKYSGDNEPL
CCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECEEEEECCCCCCCCCCC
EHDCPCPACTNYTKAYLHHLVRIGEILGSMLMTWHNLTYFQNLISRIREYIKLGKDFDFY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
S
C
>Mature Secondary Structure 
SKFSFNIHHQHKKARSGIIATAHGEIRTPAFMPVGTRGTVKAMLPESVAETGADILLGN
CCEEEEHHHHHHHHHCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEECC
TYHLMLQPSAERIARLGGLHKFMNWDKPILTDSGGFQVMSLSKLRKITEEGVSFSSHING
EEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHCCCHHHCCCC
DKYMLTPERSTAIQYLLGSTITMAFDECTPYPVTFEEAKTSMQLTTRWAHRSRNAFVKRE
CEEEECCCHHHHHHHHHCCHHEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEECC
GYAQFGIIQGSVYEELREQSAKGLVELDFDGYAIGGLAVGEGQELMFKVLDYAPDFLPQN
CCCEEEEEECHHHHHHHHHHCCCEEEECCCCEEECCEEECCCHHHHHHHHHHCHHHCCCC
KPRYLMGVGKPADIIGAVNRGIDMFDCVIPTRSGRNGQAFTKYGTVNICNSKYSGDNEPL
CCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECEEEEECCCCCCCCCCC
EHDCPCPACTNYTKAYLHHLVRIGEILGSMLMTWHNLTYFQNLISRIREYIKLGKDFDFY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA