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The map label for this gene is ligA

Identifier: 157826142

GI number: 157826142

Start: 994917

End: 996986

Strand: Reverse

Name: ligA

Synonym: A1C_05595

Alternate gene names: 157826142

Gene position: 996986-994917 (Counterclockwise)

Preceding gene: 157826143

Following gene: 157826141

Centisome position: 80.99

GC content: 35.27

Gene sequence:

>2070_bases
ATGCAAAATATTGATTTTATATCTGAAGCAGAAGCAAAAAAATTACTAGAAGAACTCAGCTGTAAAATAGCAGCGTATAA
CCATGCTTATTATATAGAGGATAATCCTTTAGTGTCAGATGCCGAGTATGATCAGCTAGTTAATATCAATCTAAAATTAG
AGCAGCAATTTCCTCATTTAGTTTTAGAAAATAGTCCTAGCAAGGAAGTAGGGGCTAAAATAGCAAATAAATTTGTTAAA
GTTACGCATCAAGTGCCTATGCTATCTCTTAGTAATGCTTTTGATGAGCAAGATGTAAGAGATTTTGTAGACCGTATAAA
AAATTTCTTACGTCTTGATGAGTTTGCTCCTATCTTTTGTGAACCTAAAATAGATGGCTTATCTTTTTCTGCTATATATA
AAAACGGGCTGCTTATAACAGGAGCGACCAGAGGTGATGGATATGTCGGTGAAGATATAACGGCAAATATTAAAACAATC
AAAAATTTTCCTCATAAAATAGATAATGCTCCGGAATGTTTAGAAGTACGAGGGGAAATTTATATTGAAAAACAAGATTT
TTTAAACCTAAATGAAGAACAAGAAGAGCAAGGCAGAGATCAATTTGCTAACCCCCGTAATGCCGCCGCCGGTTCGCTTC
GTCAGCTTGACGCTTCTATTACGGCTAAACGACCGCTCAAATATTTTGTCTATTCAGGTGGAGTAACTGAGCAGAATCTG
GCTTCTTCTCAAGATCAATTACTTACAAAATTAAAAAAATTTGGTTTTAGCGTGAATGAAATATCTAAGCTTACAAAGTC
CGAAGAAGAAATTTTTGCCTTTTACGAATATTTAAAAACAAATCGAGAAAATTTACCTTATGAAATTGATGGGGTAGTAT
ATAAGCTAAATGATTTTGAACTACAAAATAGAATGGGTTTTATTGCTCGATCTCCACGTTTTGCTACCGCTCATAAATTT
CCCGCAATAATAGGACAAACAAAACTGCTCTCTATTACTGTGCAGGTCGGTAGAACCGGTACGTTAACGCCGGTAGCAGA
GCTTGAGCCTATAGAAATAGGCGGGGTAACCGTTAGCAGAGCAACGCTGCATAATTTCCAAGAAATCGTACGAAAAGATG
TGCAGATTAAGGATTACGTATTCTTGCAGCGTGCCGGTGACGTTATTCCTAAAATCACGGGAGCTGATATAGAGAAGCGT
CCTAATGATACAATAGCATTTGAAGCTCCTTTATTTTGTCCTTCATGTAATTCTAAGTTGTATTACGTTCCTGAAGATAT
TATTATACGCTGTGATAACGGTCTTAATTGTCCAGCTCAGCATTATGAGCGTATACGCCATTTTGTATCAAAAAATGCGA
TGGATATTGCAGGGCTTGGGCGTAAGCAAGTTGAGTTTTTAATAGCTAAGGGGTTGATTAGTAATCCTCTCGATATTTTC
TTTTTAAAAAAAAACAATGAAGCTAGTTTAATCAAACTTGAAAATATGGATGGATGGGGTCAAAAATCAGTAGAAAAGCT
TTTGAAAAATATAGAAAAATCAAAAAATGTTAGCTTGCCAAGATTTATATATGCACTAGGCATAAGGCATATTGGTGAAC
AAAATGCTAAATTACTTGCTAGAGAATTTGGCAGCTATAATAATTTTATAGCTCAAATGGAATTGCTTAGCAAAAACGAT
TCGGATATCTATCAAAAACTAAACAATTTAGAAGGCATCGGTGATAAGATTTTAATAGATATTATTGCCTTTTTGGACGT
TAAAGAAAATATCCAGCTTATCAAAAAACTTGGTGAAATATTAAATATTGAAGATTATAAAGAGACTAGGGCACAAAGCA
GTTTAACAGGTAAGATAGTGGTATTTACCGGTAGCTTACCGACGATATCTAGAGCAGAAGTTAAAGCCACAGCTGAGAAG
CTTGGAGCTAAAGTTGCGGCTAGCGTATCATCGAACACGGATTTAGTGGTAGCAGGCCTTGATGCAGGCAGTAAGCTTCA
AAAAGCTAAAGAGCTTAATATTAAAATTATTGATGAAGAAGAATGGCTTACGCTTATAAAAAACGCTTAG

Upstream 100 bases:

>100_bases
CTTCTCGCAATGACGAAAAACCGGTCCACGCAACAACGCCTCCTCGCAATGACAAAATTTATAGTTATTGAGCAATGTAA
ATTAAAAATATTAACTACTA

Downstream 100 bases:

>100_bases
GAAAAGCAACTGTTGCATGTTTTGCAACAGTTCAAATGGACGGAGAAAAGAGTGTCAAAAGACAAATAGAATTATAATTT
AGATTCAATAATCTTAGTAG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MQNIDFISEAEAKKLLEELSCKIAAYNHAYYIEDNPLVSDAEYDQLVNINLKLEQQFPHLVLENSPSKEVGAKIANKFVK
VTHQVPMLSLSNAFDEQDVRDFVDRIKNFLRLDEFAPIFCEPKIDGLSFSAIYKNGLLITGATRGDGYVGEDITANIKTI
KNFPHKIDNAPECLEVRGEIYIEKQDFLNLNEEQEEQGRDQFANPRNAAAGSLRQLDASITAKRPLKYFVYSGGVTEQNL
ASSQDQLLTKLKKFGFSVNEISKLTKSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFELQNRMGFIARSPRFATAHKF
PAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSRATLHNFQEIVRKDVQIKDYVFLQRAGDVIPKITGADIEKR
PNDTIAFEAPLFCPSCNSKLYYVPEDIIIRCDNGLNCPAQHYERIRHFVSKNAMDIAGLGRKQVEFLIAKGLISNPLDIF
FLKKNNEASLIKLENMDGWGQKSVEKLLKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLAREFGSYNNFIAQMELLSKND
SDIYQKLNNLEGIGDKILIDIIAFLDVKENIQLIKKLGEILNIEDYKETRAQSSLTGKIVVFTGSLPTISRAEVKATAEK
LGAKVAASVSSNTDLVVAGLDAGSKLQKAKELNIKIIDEEEWLTLIKNA

Sequences:

>Translated_689_residues
MQNIDFISEAEAKKLLEELSCKIAAYNHAYYIEDNPLVSDAEYDQLVNINLKLEQQFPHLVLENSPSKEVGAKIANKFVK
VTHQVPMLSLSNAFDEQDVRDFVDRIKNFLRLDEFAPIFCEPKIDGLSFSAIYKNGLLITGATRGDGYVGEDITANIKTI
KNFPHKIDNAPECLEVRGEIYIEKQDFLNLNEEQEEQGRDQFANPRNAAAGSLRQLDASITAKRPLKYFVYSGGVTEQNL
ASSQDQLLTKLKKFGFSVNEISKLTKSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFELQNRMGFIARSPRFATAHKF
PAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSRATLHNFQEIVRKDVQIKDYVFLQRAGDVIPKITGADIEKR
PNDTIAFEAPLFCPSCNSKLYYVPEDIIIRCDNGLNCPAQHYERIRHFVSKNAMDIAGLGRKQVEFLIAKGLISNPLDIF
FLKKNNEASLIKLENMDGWGQKSVEKLLKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLAREFGSYNNFIAQMELLSKND
SDIYQKLNNLEGIGDKILIDIIAFLDVKENIQLIKKLGEILNIEDYKETRAQSSLTGKIVVFTGSLPTISRAEVKATAEK
LGAKVAASVSSNTDLVVAGLDAGSKLQKAKELNIKIIDEEEWLTLIKNA
>Mature_689_residues
MQNIDFISEAEAKKLLEELSCKIAAYNHAYYIEDNPLVSDAEYDQLVNINLKLEQQFPHLVLENSPSKEVGAKIANKFVK
VTHQVPMLSLSNAFDEQDVRDFVDRIKNFLRLDEFAPIFCEPKIDGLSFSAIYKNGLLITGATRGDGYVGEDITANIKTI
KNFPHKIDNAPECLEVRGEIYIEKQDFLNLNEEQEEQGRDQFANPRNAAAGSLRQLDASITAKRPLKYFVYSGGVTEQNL
ASSQDQLLTKLKKFGFSVNEISKLTKSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFELQNRMGFIARSPRFATAHKF
PAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSRATLHNFQEIVRKDVQIKDYVFLQRAGDVIPKITGADIEKR
PNDTIAFEAPLFCPSCNSKLYYVPEDIIIRCDNGLNCPAQHYERIRHFVSKNAMDIAGLGRKQVEFLIAKGLISNPLDIF
FLKKNNEASLIKLENMDGWGQKSVEKLLKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLAREFGSYNNFIAQMELLSKND
SDIYQKLNNLEGIGDKILIDIIAFLDVKENIQLIKKLGEILNIEDYKETRAQSSLTGKIVVFTGSLPTISRAEVKATAEK
LGAKVAASVSSNTDLVVAGLDAGSKLQKAKELNIKIIDEEEWLTLIKNA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=683, Percent_Identity=42.0204978038067, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI87082305, Length=601, Percent_Identity=22.6289517470882, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324791, Length=73, Percent_Identity=50.6849315068493, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_RICAH (A8GPM9)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493862.1
- ProteinModelPortal:   A8GPM9
- STRING:   A8GPM9
- GeneID:   5644750
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_05595
- NMPDR:   fig|293614.3.peg.1055
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   RAKA293614:A1C_05595-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77422; Mature: 77422

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 123-123 BINDING 121-121 BINDING 144-144 BINDING 179-179 BINDING 295-295 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNIDFISEAEAKKLLEELSCKIAAYNHAYYIEDNPLVSDAEYDQLVNINLKLEQQFPHL
CCCCCCCHHHHHHHHHHHHCCEEEEECEEEEECCCCCCCCCCCCEEEEEEEEEECCCCCE
VLENSPSKEVGAKIANKFVKVTHQVPMLSLSNAFDEQDVRDFVDRIKNFLRLDEFAPIFC
EECCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
EPKIDGLSFSAIYKNGLLITGATRGDGYVGEDITANIKTIKNFPHKIDNAPECLEVRGEI
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHCCCHHCCCCCHHHEECCEE
YIEKQDFLNLNEEQEEQGRDQFANPRNAAAGSLRQLDASITAKRPLKYFVYSGGVTEQNL
EEEHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCCCEEEEEEECCCCHHHH
ASSQDQLLTKLKKFGFSVNEISKLTKSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFE
HCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCEEEEECCEE
LQNRMGFIARSPRFATAHKFPAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSR
HHHCCCHHCCCCCCCHHHHCHHHHCCCEEEEEEEEECCCCCCCCCCCCCCEEECCEEEHH
ATLHNFQEIVRKDVQIKDYVFLQRAGDVIPKITGADIEKRPNDTIAFEAPLFCPSCNSKL
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCEECCCCCCEE
YYVPEDIIIRCDNGLNCPAQHYERIRHFVSKNAMDIAGLGRKQVEFLIAKGLISNPLDIF
EECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCEEE
FLKKNNEASLIKLENMDGWGQKSVEKLLKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLA
EEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
REFGSYNNFIAQMELLSKNDSDIYQKLNNLEGIGDKILIDIIAFLDVKENIQLIKKLGEI
HHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LNIEDYKETRAQSSLTGKIVVFTGSLPTISRAEVKATAEKLGAKVAASVSSNTDLVVAGL
CCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHEEECCCCCCEEEEEC
DAGSKLQKAKELNIKIIDEEEWLTLIKNA
CCCHHHHHHHCCCEEEECCHHHHHHHCCC
>Mature Secondary Structure
MQNIDFISEAEAKKLLEELSCKIAAYNHAYYIEDNPLVSDAEYDQLVNINLKLEQQFPHL
CCCCCCCHHHHHHHHHHHHCCEEEEECEEEEECCCCCCCCCCCCEEEEEEEEEECCCCCE
VLENSPSKEVGAKIANKFVKVTHQVPMLSLSNAFDEQDVRDFVDRIKNFLRLDEFAPIFC
EECCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
EPKIDGLSFSAIYKNGLLITGATRGDGYVGEDITANIKTIKNFPHKIDNAPECLEVRGEI
CCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHCCCHHCCCCCHHHEECCEE
YIEKQDFLNLNEEQEEQGRDQFANPRNAAAGSLRQLDASITAKRPLKYFVYSGGVTEQNL
EEEHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCCCEEEEEEECCCCHHHH
ASSQDQLLTKLKKFGFSVNEISKLTKSEEEIFAFYEYLKTNRENLPYEIDGVVYKLNDFE
HCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEECCEEEEECCEE
LQNRMGFIARSPRFATAHKFPAIIGQTKLLSITVQVGRTGTLTPVAELEPIEIGGVTVSR
HHHCCCHHCCCCCCCHHHHCHHHHCCCEEEEEEEEECCCCCCCCCCCCCCEEECCEEEHH
ATLHNFQEIVRKDVQIKDYVFLQRAGDVIPKITGADIEKRPNDTIAFEAPLFCPSCNSKL
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCEECCCCCCEE
YYVPEDIIIRCDNGLNCPAQHYERIRHFVSKNAMDIAGLGRKQVEFLIAKGLISNPLDIF
EECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCEEE
FLKKNNEASLIKLENMDGWGQKSVEKLLKNIEKSKNVSLPRFIYALGIRHIGEQNAKLLA
EEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
REFGSYNNFIAQMELLSKNDSDIYQKLNNLEGIGDKILIDIIAFLDVKENIQLIKKLGEI
HHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LNIEDYKETRAQSSLTGKIVVFTGSLPTISRAEVKATAEKLGAKVAASVSSNTDLVVAGL
CCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHEEECCCCCCEEEEEC
DAGSKLQKAKELNIKIIDEEEWLTLIKNA
CCCHHHHHHHCCCEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA