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The map label for this gene is proP [H]

Identifier: 157826104

GI number: 157826104

Start: 951095

End: 952375

Strand: Direct

Name: proP [H]

Synonym: A1C_05340

Alternate gene names: 157826104

Gene position: 951095-952375 (Clockwise)

Preceding gene: 157826098

Following gene: 157826106

Centisome position: 77.26

GC content: 36.07

Gene sequence:

>1281_bases
ATGAGCAATTCATTAAGCAAGAGGGATTTTTCTATCATAATCGGTAATGCGATGGACCATTTTGATACTGCACTTTATGG
GTTTCTTGTACCGTTACTTGCTGGTATTTTCTTTCCGCATCATGATAAAATTGTCGCCTTAATACTTACCTATAGTGTTC
TTGCGACATCTTTATTTACACGTCCCATAGGCTCTTATTGTTTTGGCGTAGTTGCTAAAAAATACGGCGGGGTTTTTGCT
TTATCTCATTCTCTAATCGGAGTTGCTTTAACAACTAGCTTAATAGGTCTAATCCCCTCTCATACGCACATAGGCTGGCT
TGCACCGCTATTATTAGTTGTGCTTAGAACGTTGCAAGGAGTATATTCCGAGGGTGAATGTGCTATTGCTAAATTATATA
TTCTAGAGAATAAAGACGAAAAAAAAGCATTTAAAGCTTCTTACCTTTATCAAACCTCTACAATGCTTGGTATTATTCTT
GCTTCATTTATCAGCAGTATAGTTTTAAATCTAGAGTATAATGAATATTGGCGTTTATGTTTCATATTTGGCGGACTTAT
AGCCCTTATAGGTTATTTTTTAAGGAAGAGCGAGGATATTATAGTAAGACCATCATTGTCAAGCCACGGGCTGACAATGA
GATCCTCGCCTCGCAATGACGTGGTATCATCGCTATTACTCGACTTAAACACAATTTGGAACAATAAACTAAGTATCTTA
CGTATTAGTTTTGCCGTAGGTTTCTCATATATTACCTATATAGTGCCTTGCGTCTTTATGAATAGCTTTATTCCGCTTAT
CACTGATATATCGCTTGAAACAATGATGAAATCTAATACGGAATTTCTGATTTGTGATATGATTATGATTCCGATAATTG
GTCATCTAACAAAAAAAGTCCATTACCTTAAAATATTGAAGGGTACTCTCATTATCATGAGTTTAAGCATAATTCCTTTA
TGGTTTTTCTTAGATAATTCGTCTATATGGTATGTTAATTTCGTACGTATTTGGATTATAATACTCGGTGTTGGCTTCTT
AGCACCATTGAATTGCTGGCTAAATAATTTGTTTAAAACTGCCGATAAATATATGTTAGTAGGTATCGGCAGTAGTATAG
GCTCTTCACTGATCGGACGCCTTACCCCGTCAATCTGCCTAATGCTTTGGCATGTAACCGGCATCTCTTTATCAATTGCG
GTTTACATAACTGTAATATCAATGGTAACCTTATGGGCTGTTAGAGGAGTTATTGCACAGGTCAATGTCATGCATGCATA
G

Upstream 100 bases:

>100_bases
CGTATATACCTAACGTGCGGATCAAAAGCGAAAAACAACGACGCCAATTTGTCAAATTCAGCAAGTATACATTATAAATT
TTACATTACATTATTAATAT

Downstream 100 bases:

>100_bases
GCGTGGCTTTGCTTATAGTACTGGACAGTTTTTACTTTATGTCATTTCTGCGGAAGTGGTCATCCAGAAAAAAGCATCAC
TACAGCAAGCTTTTTGAATT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSNSLSKRDFSIIIGNAMDHFDTALYGFLVPLLAGIFFPHHDKIVALILTYSVLATSLFTRPIGSYCFGVVAKKYGGVFA
LSHSLIGVALTTSLIGLIPSHTHIGWLAPLLLVVLRTLQGVYSEGECAIAKLYILENKDEKKAFKASYLYQTSTMLGIIL
ASFISSIVLNLEYNEYWRLCFIFGGLIALIGYFLRKSEDIIVRPSLSSHGLTMRSSPRNDVVSSLLLDLNTIWNNKLSIL
RISFAVGFSYITYIVPCVFMNSFIPLITDISLETMMKSNTEFLICDMIMIPIIGHLTKKVHYLKILKGTLIIMSLSIIPL
WFFLDNSSIWYVNFVRIWIIILGVGFLAPLNCWLNNLFKTADKYMLVGIGSSIGSSLIGRLTPSICLMLWHVTGISLSIA
VYITVISMVTLWAVRGVIAQVNVMHA

Sequences:

>Translated_426_residues
MSNSLSKRDFSIIIGNAMDHFDTALYGFLVPLLAGIFFPHHDKIVALILTYSVLATSLFTRPIGSYCFGVVAKKYGGVFA
LSHSLIGVALTTSLIGLIPSHTHIGWLAPLLLVVLRTLQGVYSEGECAIAKLYILENKDEKKAFKASYLYQTSTMLGIIL
ASFISSIVLNLEYNEYWRLCFIFGGLIALIGYFLRKSEDIIVRPSLSSHGLTMRSSPRNDVVSSLLLDLNTIWNNKLSIL
RISFAVGFSYITYIVPCVFMNSFIPLITDISLETMMKSNTEFLICDMIMIPIIGHLTKKVHYLKILKGTLIIMSLSIIPL
WFFLDNSSIWYVNFVRIWIIILGVGFLAPLNCWLNNLFKTADKYMLVGIGSSIGSSLIGRLTPSICLMLWHVTGISLSIA
VYITVISMVTLWAVRGVIAQVNVMHA
>Mature_425_residues
SNSLSKRDFSIIIGNAMDHFDTALYGFLVPLLAGIFFPHHDKIVALILTYSVLATSLFTRPIGSYCFGVVAKKYGGVFAL
SHSLIGVALTTSLIGLIPSHTHIGWLAPLLLVVLRTLQGVYSEGECAIAKLYILENKDEKKAFKASYLYQTSTMLGIILA
SFISSIVLNLEYNEYWRLCFIFGGLIALIGYFLRKSEDIIVRPSLSSHGLTMRSSPRNDVVSSLLLDLNTIWNNKLSILR
ISFAVGFSYITYIVPCVFMNSFIPLITDISLETMMKSNTEFLICDMIMIPIIGHLTKKVHYLKILKGTLIIMSLSIIPLW
FFLDNSSIWYVNFVRIWIIILGVGFLAPLNCWLNNLFKTADKYMLVGIGSSIGSSLIGRLTPSICLMLWHVTGISLSIAV
YITVISMVTLWAVRGVIAQVNVMHA

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=423, Percent_Identity=26.7139479905437, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788292, Length=409, Percent_Identity=24.4498777506112, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788942, Length=420, Percent_Identity=23.0952380952381, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789941, Length=201, Percent_Identity=31.3432835820896, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47450; Mature: 47319

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSLSKRDFSIIIGNAMDHFDTALYGFLVPLLAGIFFPHHDKIVALILTYSVLATSLFT
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RPIGSYCFGVVAKKYGGVFALSHSLIGVALTTSLIGLIPSHTHIGWLAPLLLVVLRTLQG
HHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VYSEGECAIAKLYILENKDEKKAFKASYLYQTSTMLGIILASFISSIVLNLEYNEYWRLC
HCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHH
FIFGGLIALIGYFLRKSEDIIVRPSLSSHGLTMRSSPRNDVVSSLLLDLNTIWNNKLSIL
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEE
RISFAVGFSYITYIVPCVFMNSFIPLITDISLETMMKSNTEFLICDMIMIPIIGHLTKKV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
HYLKILKGTLIIMSLSIIPLWFFLDNSSIWYVNFVRIWIIILGVGFLAPLNCWLNNLFKT
HHHHHHHHHHHHHHHHHHHEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKYMLVGIGSSIGSSLIGRLTPSICLMLWHVTGISLSIAVYITVISMVTLWAVRGVIAQ
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
VNVMHA
HHHCCC
>Mature Secondary Structure 
SNSLSKRDFSIIIGNAMDHFDTALYGFLVPLLAGIFFPHHDKIVALILTYSVLATSLFT
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RPIGSYCFGVVAKKYGGVFALSHSLIGVALTTSLIGLIPSHTHIGWLAPLLLVVLRTLQG
HHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VYSEGECAIAKLYILENKDEKKAFKASYLYQTSTMLGIILASFISSIVLNLEYNEYWRLC
HCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHH
FIFGGLIALIGYFLRKSEDIIVRPSLSSHGLTMRSSPRNDVVSSLLLDLNTIWNNKLSIL
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEE
RISFAVGFSYITYIVPCVFMNSFIPLITDISLETMMKSNTEFLICDMIMIPIIGHLTKKV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
HYLKILKGTLIIMSLSIIPLWFFLDNSSIWYVNFVRIWIIILGVGFLAPLNCWLNNLFKT
HHHHHHHHHHHHHHHHHHHEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKYMLVGIGSSIGSSLIGRLTPSICLMLWHVTGISLSIAVYITVISMVTLWAVRGVIAQ
CCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
VNVMHA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA