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The map label for this gene is xthA [H]

Identifier: 157826086

GI number: 157826086

Start: 935014

End: 935796

Strand: Direct

Name: xthA [H]

Synonym: A1C_05230

Alternate gene names: 157826086

Gene position: 935014-935796 (Clockwise)

Preceding gene: 157826085

Following gene: 157826087

Centisome position: 75.95

GC content: 30.4

Gene sequence:

>783_bases
ATGAAAATAGCTACTTGGAATATTAATTCCATAAAAACAAGACTTAATTTATTGCGTAATTTTTTATCTCAAGAAAATCC
GGATATTTTATTATTACAAGAAATAAAATGCAAAACCGAAAATTTTCCTTTTGATGAATTATCCGATCTACCTTATAATT
TTTATGTTCACGGACAACAATCATATAACGGCGTTGCTATAATTTCAAAATTTCCTGCGGATGAAATAATTAAGGATTTT
CCGAATAATTACTGCAGTGATCAAGCAAGATTCTTAGAAATAAAATTATCATTACCTATAGGGTTTTGTAATATAATCTC
GCTCTATGCTCCTAACGGTTCATTTGTCGGTAGCGATAAATTTGTTGCAAAGCTAGCATTTTATGATAATTTTATCAATT
ATCTATCAACTAAAAAATCTTTTGACGAAAAAACTATCATAGGAGGAGATCTCAATATTGCACCGTTCGATATAGACGTA
TATTCGCCTAAGATACTTTCTGAAACTACTTGTTTTACTGAGGTTGAACAAAAAAAACTACGTACTATTCTAAATGCCGG
GTTTGAGGATTTATATAGATTGATGCATCCGAGTAAACAAGCATTTTCATGGTGGGATTATAGAGCCGGATGTTTTGAAC
AAAATAAAGGTATGCGAATTGATATGATTCTTGGTTGTAATAATACTATTGACTATTTAGAAAATTGCTATATGGATTAT
AATTTAAGGACCCAAGAAAAACCTTCAGATCATATACCGGTAATTGCAAGCTTTAAGGTGTAG

Upstream 100 bases:

>100_bases
TAGTAATTTCCGGAGCATTTATTACTAGTCTCTTAGTTTATCATTTTGGTTTTCTTGAATGGCGATTCCGTAATGAAAGT
CCAAACTTAGAAAAACCAAC

Downstream 100 bases:

>100_bases
CATGAGTCGTCATTGTCTGCATGGACCGAAAAACGCACTCGGTGTCGTACCGTGGCTTGACCACGGTATCCATAAAAATA
ATTTAAAATACTAATATTAG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKIATWNINSIKTRLNLLRNFLSQENPDILLLQEIKCKTENFPFDELSDLPYNFYVHGQQSYNGVAIISKFPADEIIKDF
PNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDKFVAKLAFYDNFINYLSTKKSFDEKTIIGGDLNIAPFDIDV
YSPKILSETTCFTEVEQKKLRTILNAGFEDLYRLMHPSKQAFSWWDYRAGCFEQNKGMRIDMILGCNNTIDYLENCYMDY
NLRTQEKPSDHIPVIASFKV

Sequences:

>Translated_260_residues
MKIATWNINSIKTRLNLLRNFLSQENPDILLLQEIKCKTENFPFDELSDLPYNFYVHGQQSYNGVAIISKFPADEIIKDF
PNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDKFVAKLAFYDNFINYLSTKKSFDEKTIIGGDLNIAPFDIDV
YSPKILSETTCFTEVEQKKLRTILNAGFEDLYRLMHPSKQAFSWWDYRAGCFEQNKGMRIDMILGCNNTIDYLENCYMDY
NLRTQEKPSDHIPVIASFKV
>Mature_260_residues
MKIATWNINSIKTRLNLLRNFLSQENPDILLLQEIKCKTENFPFDELSDLPYNFYVHGQQSYNGVAIISKFPADEIIKDF
PNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDKFVAKLAFYDNFINYLSTKKSFDEKTIIGGDLNIAPFDIDV
YSPKILSETTCFTEVEQKKLRTILNAGFEDLYRLMHPSKQAFSWWDYRAGCFEQNKGMRIDMILGCNNTIDYLENCYMDY
NLRTQEKPSDHIPVIASFKV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=23.0483271375465, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=23.0483271375465, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=23.0483271375465, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=33.7078651685393, Blast_Score=164, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=26.2357414448669, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI221330655, Length=261, Percent_Identity=26.0536398467433, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136678, Length=261, Percent_Identity=26.0536398467433, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30061; Mature: 30061

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNINSIKTRLNLLRNFLSQENPDILLLQEIKCKTENFPFDELSDLPYNFYVHGQQ
CEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHCCCEEEEEECCC
SYNGVAIISKFPADEIIKDFPNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDK
CCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCEECCHH
FVAKLAFYDNFINYLSTKKSFDEKTIIGGDLNIAPFDIDVYSPKILSETTCFTEVEQKKL
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEECCCCHHHCCHHHHHHHHHHHH
RTILNAGFEDLYRLMHPSKQAFSWWDYRAGCFEQNKGMRIDMILGCNNTIDYLENCYMDY
HHHHHCCHHHHHHHHCCCCHHCCHHCCCCCCEECCCCCEEEEEEECCCHHHHHHHCEECC
NLRTQEKPSDHIPVIASFKV
CCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKIATWNINSIKTRLNLLRNFLSQENPDILLLQEIKCKTENFPFDELSDLPYNFYVHGQQ
CEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHCCCEEEEEECCC
SYNGVAIISKFPADEIIKDFPNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDK
CCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCEECCHH
FVAKLAFYDNFINYLSTKKSFDEKTIIGGDLNIAPFDIDVYSPKILSETTCFTEVEQKKL
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEECCCCHHHCCHHHHHHHHHHHH
RTILNAGFEDLYRLMHPSKQAFSWWDYRAGCFEQNKGMRIDMILGCNNTIDYLENCYMDY
HHHHHCCHHHHHHHHCCCCHHCCHHCCCCCCEECCCCCEEEEEEECCCHHHHHHHCEECC
NLRTQEKPSDHIPVIASFKV
CCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]