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The map label for this gene is gltX

Identifier: 157826025

GI number: 157826025

Start: 885207

End: 886598

Strand: Direct

Name: gltX

Synonym: A1C_04895

Alternate gene names: 157826025

Gene position: 885207-886598 (Clockwise)

Preceding gene: 157826024

Following gene: 157826026

Centisome position: 71.91

GC content: 37.43

Gene sequence:

>1392_bases
ATGACCAACGTAATTACTAGATTTGCTCCGTCACCGACGGGGTTTTTACATATAGGTTCGGCAAGAACTGCCCTTTTTAA
CTACTTGTTCGCAAGGCATAATAACGGTAAGTTTCTGCTCCGCATTGAAGATACCGATAAAGAAAGGTCAACTAAAGCAG
CGGTAGACGCTATATTTTCAGGTCTAAAATGGCTAGGGCTTGATTGGGACGCGGAAGTTATATTCCAGTCTAAACGTAAT
GATCTTTACAAAAAAGCGGCTCTAAGACTCCTGAAAGAAGGTAAGGCATATTATTGTTTTACTAGCCAAGAAGAAATAGA
AAAGCAGCGACAAAAAGCTTTAGAAAATAAACAGCATTTCATTTTTAATAGCGAGTGGCGTGATAAAGAACCTGACGCCT
ATCCTACTGATGTTAAGCCAGTAATACGTCTCAAAACTCCAAGAGAGGGCAGAATAACAATTCATGATACCTTACAAGGC
GAGGTAGTAATTGAGAACTCTCATATAGACGATATGGTACTACTAAGGGCAGATGGCACTGCTACTTACATGCTAGCCGT
AGTAGCAGACGATCATGATATGGGTATCACTCATATTATTAGAGGTGACGATCATTTAACCAATGCAGCAAGGCAAATTG
CTATTTATCAGGCTCTCGGTTATGAAGTACCGAGCATGACTCATATACCGCTAATTCATGGAGCAGACGGAGCAAAACTA
TCAAAAAGACACGGAGCCTTAGGCGTTGACGCTTATAAAAATATGGGATATTTACCTGAAAGTTTGTGTAATTATTTATT
GCGTCTCGGATGGAGTCACGGTAATGATGAAATTATATCAATGACTCAGGCTATAGAATGGTTTAATCTTGATTCGCTCG
GTAAATCACCTTCTAGGCTTGATTTTGCTAAAATGAATAGCCTTAATGCTTATTACTTAAGAATGCTTGACAATGATACC
TTAACTGCAAAGACTGTCGAGATATTGAAACAAAATTATAAGATTAGTGATAAAGAGGTAAGTTATATAAAACAAGCAAT
GTCAAGCTTGCTAGTTAGAAGCGAAACATTGCTAGATTTAGCACAGCTTGCTCAAATTTATCTCGTAGATTCGCCCATCG
TCTACAACCAAGATGCAAAGGAAATTATAGAAAATTGCAATAAAGATTTGATCAAGCAAGTTACAGAAGGTTTAAACAAG
ATTGAGAAGTTTGATAAGGAATCCGTACAGAATAAATTTAAAGAAATAGCAGCAGCAAACGACTTAAAGCTGAGTGACAT
TATGAAGCCCGTAAGAGCTTTAATAACCGGCATGGACGCATCACCTAGCGTATTTGCAATTGCAGAAATTTTAGGAAAAG
AAAATATTTTAAAAAGGTTAGAAACTATATGA

Upstream 100 bases:

>100_bases
ATTCCTGCTTTAGCAGGAATGACATCTTGAACGTTCATACACTAGGACATCAAGACACGGTATGACACAGTTCTTAGTAT
ATATTTAAAAACAATAATAC

Downstream 100 bases:

>100_bases
AAGATTGTATAAAAAAAGTTGCTTTTGTTCTTAGTGGATTATTTATAATAACCAGTACTTTTATTTTATACAGAGTAACA
AATGTCGATGCTTCATCAAC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTNVITRFAPSPTGFLHIGSARTALFNYLFARHNNGKFLLRIEDTDKERSTKAAVDAIFSGLKWLGLDWDAEVIFQSKRN
DLYKKAALRLLKEGKAYYCFTSQEEIEKQRQKALENKQHFIFNSEWRDKEPDAYPTDVKPVIRLKTPREGRITIHDTLQG
EVVIENSHIDDMVLLRADGTATYMLAVVADDHDMGITHIIRGDDHLTNAARQIAIYQALGYEVPSMTHIPLIHGADGAKL
SKRHGALGVDAYKNMGYLPESLCNYLLRLGWSHGNDEIISMTQAIEWFNLDSLGKSPSRLDFAKMNSLNAYYLRMLDNDT
LTAKTVEILKQNYKISDKEVSYIKQAMSSLLVRSETLLDLAQLAQIYLVDSPIVYNQDAKEIIENCNKDLIKQVTEGLNK
IEKFDKESVQNKFKEIAAANDLKLSDIMKPVRALITGMDASPSVFAIAEILGKENILKRLETI

Sequences:

>Translated_463_residues
MTNVITRFAPSPTGFLHIGSARTALFNYLFARHNNGKFLLRIEDTDKERSTKAAVDAIFSGLKWLGLDWDAEVIFQSKRN
DLYKKAALRLLKEGKAYYCFTSQEEIEKQRQKALENKQHFIFNSEWRDKEPDAYPTDVKPVIRLKTPREGRITIHDTLQG
EVVIENSHIDDMVLLRADGTATYMLAVVADDHDMGITHIIRGDDHLTNAARQIAIYQALGYEVPSMTHIPLIHGADGAKL
SKRHGALGVDAYKNMGYLPESLCNYLLRLGWSHGNDEIISMTQAIEWFNLDSLGKSPSRLDFAKMNSLNAYYLRMLDNDT
LTAKTVEILKQNYKISDKEVSYIKQAMSSLLVRSETLLDLAQLAQIYLVDSPIVYNQDAKEIIENCNKDLIKQVTEGLNK
IEKFDKESVQNKFKEIAAANDLKLSDIMKPVRALITGMDASPSVFAIAEILGKENILKRLETI
>Mature_462_residues
TNVITRFAPSPTGFLHIGSARTALFNYLFARHNNGKFLLRIEDTDKERSTKAAVDAIFSGLKWLGLDWDAEVIFQSKRND
LYKKAALRLLKEGKAYYCFTSQEEIEKQRQKALENKQHFIFNSEWRDKEPDAYPTDVKPVIRLKTPREGRITIHDTLQGE
VVIENSHIDDMVLLRADGTATYMLAVVADDHDMGITHIIRGDDHLTNAARQIAIYQALGYEVPSMTHIPLIHGADGAKLS
KRHGALGVDAYKNMGYLPESLCNYLLRLGWSHGNDEIISMTQAIEWFNLDSLGKSPSRLDFAKMNSLNAYYLRMLDNDTL
TAKTVEILKQNYKISDKEVSYIKQAMSSLLVRSETLLDLAQLAQIYLVDSPIVYNQDAKEIIENCNKDLIKQVTEGLNKI
EKFDKESVQNKFKEIAAANDLKLSDIMKPVRALITGMDASPSVFAIAEILGKENILKRLETI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=31.006160164271, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI4826960, Length=171, Percent_Identity=30.9941520467836, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788743, Length=457, Percent_Identity=36.7614879649891, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI226510925, Length=250, Percent_Identity=37.2, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1786895, Length=128, Percent_Identity=32.03125, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=31.5352697095436, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=491, Percent_Identity=33.6048879837067, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324742, Length=108, Percent_Identity=36.1111111111111, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=110, Percent_Identity=34.5454545454545, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=31.9105691056911, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24649466, Length=118, Percent_Identity=33.0508474576271, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_RICAH (A8GPB2)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493745.1
- ProteinModelPortal:   A8GPB2
- SMR:   A8GPB2
- STRING:   A8GPB2
- GeneID:   5645637
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_04895
- NMPDR:   fig|293614.3.peg.915
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- ProtClustDB:   PRK01406
- BioCyc:   RAKA293614:A1C_04895-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52434; Mature: 52303

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVITRFAPSPTGFLHIGSARTALFNYLFARHNNGKFLLRIEDTDKERSTKAAVDAIFS
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHHH
GLKWLGLDWDAEVIFQSKRNDLYKKAALRLLKEGKAYYCFTSQEEIEKQRQKALENKQHF
CHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCE
IFNSEWRDKEPDAYPTDVKPVIRLKTPREGRITIHDTLQGEVVIENSHIDDMVLLRADGT
EECCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEECCCCCCEEEEEECCC
ATYMLAVVADDHDMGITHIIRGDDHLTNAARQIAIYQALGYEVPSMTHIPLIHGADGAKL
EEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCH
SKRHGALGVDAYKNMGYLPESLCNYLLRLGWSHGNDEIISMTQAIEWFNLDSLGKSPSRL
HHHCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCC
DFAKMNSLNAYYLRMLDNDTLTAKTVEILKQNYKISDKEVSYIKQAMSSLLVRSETLLDL
CHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQLAQIYLVDSPIVYNQDAKEIIENCNKDLIKQVTEGLNKIEKFDKESVQNKFKEIAAAN
HHHHHHEEECCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLKLSDIMKPVRALITGMDASPSVFAIAEILGKENILKRLETI
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHCC
>Mature Secondary Structure 
TNVITRFAPSPTGFLHIGSARTALFNYLFARHNNGKFLLRIEDTDKERSTKAAVDAIFS
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHHH
GLKWLGLDWDAEVIFQSKRNDLYKKAALRLLKEGKAYYCFTSQEEIEKQRQKALENKQHF
CHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCE
IFNSEWRDKEPDAYPTDVKPVIRLKTPREGRITIHDTLQGEVVIENSHIDDMVLLRADGT
EECCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEECCCCCCEEEEEECCC
ATYMLAVVADDHDMGITHIIRGDDHLTNAARQIAIYQALGYEVPSMTHIPLIHGADGAKL
EEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCH
SKRHGALGVDAYKNMGYLPESLCNYLLRLGWSHGNDEIISMTQAIEWFNLDSLGKSPSRL
HHHCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCC
DFAKMNSLNAYYLRMLDNDTLTAKTVEILKQNYKISDKEVSYIKQAMSSLLVRSETLLDL
CHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQLAQIYLVDSPIVYNQDAKEIIENCNKDLIKQVTEGLNKIEKFDKESVQNKFKEIAAAN
HHHHHHEEECCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLKLSDIMKPVRALITGMDASPSVFAIAEILGKENILKRLETI
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA