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The map label for this gene is ubiF [H]

Identifier: 157825930

GI number: 157825930

Start: 781354

End: 782490

Strand: Reverse

Name: ubiF [H]

Synonym: A1C_04350

Alternate gene names: 157825930

Gene position: 782490-781354 (Counterclockwise)

Preceding gene: 157825931

Following gene: 157825929

Centisome position: 63.56

GC content: 31.84

Gene sequence:

>1137_bases
ATGAAAACAAAACAGATTGATACTATAATCTTAGGATGCGGTTTGAGTGGCATGCTAACCGCACTTTCTTTTGCAGAAAA
GGGTATTAAAACTACTATATTCGAGAGTCAATCGGTAAAAAGCCCAGAGTTTTTCAACGATATAAGAATTACTGCTTTAA
CGCCGCATTCCAAAAATTTCTTATTCTCTATTGATATATGGGCAGAGCTTGAAAAGTTTGTGGCAAAAATGCAAGAAATA
TATGTGGTAGATAATAAGGCTGCAGAGATATTAGATTTACGCAATGACGATGACTCTGTACTTGGATATGTGATTAAGAA
TAGTGATTTTAAAAAAATACTATTGTCAAGAATGACTAATAATCCGTGGATAACATTAATTGATCATAATCAATATCAAG
ATGTTATAAGCCATAATGACTACTCGATTATAAAATTTGATGATAAACACATTAAGTGTAGTTTATTAATTATATGTGAT
GGCGCAAATTCAAAAGTGAAGTCTCATTATTTTGCTAATGAAATTGAAAAATCTTATCAAACTGCTCTTACATTTAATAT
TAAGCATGAAAATCCACATGAAAATTGTGCTATGGAACATTTCCTGCCGCTTGGTCCTTTTGCTTTGCTGCCTTTAAAAA
ATCAATATGCTTCATCCGTAATATGGTCAACTTCTTCTGATCAGGCTGCTTTAATTGTTAATTTGCCTGTGGAAGAAGTT
AGATTTTTAACTCAAAGAAATGCCGGTAATTCTTTAGGTAAAATTACTATTGATAGTGAGATTAGTAGTTTTCCGCTAAA
AGCTCGTATAGCAAATAGATATTTTCATAATAAGATAGTGCTTATTGCCGACACTGCTCACACAATACATCCTTTAGCAG
GACAGGGGCTTAATCAGGGCATAAAGGATATAGAGACTTTGAGTATGATTGTTAGTAATAATGGTACATTACAAGAATAC
CAAAAGCTAAGGCAAGAGGATAATTTTATTATGTATAAGCTGACTGATGAGTTAAATAATATTTTTTCAAATTATGCCAA
AAATGTAAGATATTTAAGACAAATAGGATTTAAGGCGATAAATAATTTTAAGCCTATAAAAACCTTGATTACTGGCTATG
CGATGGGGAAGAGGTGA

Upstream 100 bases:

>100_bases
TACTCAGGCAATAGTTCATATTTGGATATAGCCATTATTTACTTTCTTTTAAGCTTTATAGCAACAAACGGATATTTAAA
ATATTTTATACATGAGTCAA

Downstream 100 bases:

>100_bases
GTGTTCCTATGTCATTACCGCGCAGGCGTGGATCCAGTAAAACATACAAAAAACTTGTTTTTTGGTTTATTTCACCAAAT
TATATAACTTCTATATAAAT

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKTKQIDTIILGCGLSGMLTALSFAEKGIKTTIFESQSVKSPEFFNDIRITALTPHSKNFLFSIDIWAELEKFVAKMQEI
YVVDNKAAEILDLRNDDDSVLGYVIKNSDFKKILLSRMTNNPWITLIDHNQYQDVISHNDYSIIKFDDKHIKCSLLIICD
GANSKVKSHYFANEIEKSYQTALTFNIKHENPHENCAMEHFLPLGPFALLPLKNQYASSVIWSTSSDQAALIVNLPVEEV
RFLTQRNAGNSLGKITIDSEISSFPLKARIANRYFHNKIVLIADTAHTIHPLAGQGLNQGIKDIETLSMIVSNNGTLQEY
QKLRQEDNFIMYKLTDELNNIFSNYAKNVRYLRQIGFKAINNFKPIKTLITGYAMGKR

Sequences:

>Translated_378_residues
MKTKQIDTIILGCGLSGMLTALSFAEKGIKTTIFESQSVKSPEFFNDIRITALTPHSKNFLFSIDIWAELEKFVAKMQEI
YVVDNKAAEILDLRNDDDSVLGYVIKNSDFKKILLSRMTNNPWITLIDHNQYQDVISHNDYSIIKFDDKHIKCSLLIICD
GANSKVKSHYFANEIEKSYQTALTFNIKHENPHENCAMEHFLPLGPFALLPLKNQYASSVIWSTSSDQAALIVNLPVEEV
RFLTQRNAGNSLGKITIDSEISSFPLKARIANRYFHNKIVLIADTAHTIHPLAGQGLNQGIKDIETLSMIVSNNGTLQEY
QKLRQEDNFIMYKLTDELNNIFSNYAKNVRYLRQIGFKAINNFKPIKTLITGYAMGKR
>Mature_378_residues
MKTKQIDTIILGCGLSGMLTALSFAEKGIKTTIFESQSVKSPEFFNDIRITALTPHSKNFLFSIDIWAELEKFVAKMQEI
YVVDNKAAEILDLRNDDDSVLGYVIKNSDFKKILLSRMTNNPWITLIDHNQYQDVISHNDYSIIKFDDKHIKCSLLIICD
GANSKVKSHYFANEIEKSYQTALTFNIKHENPHENCAMEHFLPLGPFALLPLKNQYASSVIWSTSSDQAALIVNLPVEEV
RFLTQRNAGNSLGKITIDSEISSFPLKARIANRYFHNKIVLIADTAHTIHPLAGQGLNQGIKDIETLSMIVSNNGTLQEY
QKLRQEDNFIMYKLTDELNNIFSNYAKNVRYLRQIGFKAINNFKPIKTLITGYAMGKR

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=406, Percent_Identity=26.3546798029557, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI32967307, Length=276, Percent_Identity=29.7101449275362, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786883, Length=395, Percent_Identity=26.8354430379747, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789273, Length=391, Percent_Identity=26.3427109974425, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789274, Length=391, Percent_Identity=25.3196930946292, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25146631, Length=441, Percent_Identity=25.3968253968254, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321694, Length=456, Percent_Identity=23.6842105263158, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19920760, Length=395, Percent_Identity=24.8101265822785, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42968; Mature: 42968

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKQIDTIILGCGLSGMLTALSFAEKGIKTTIFESQSVKSPEFFNDIRITALTPHSKNF
CCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCE
LFSIDIWAELEKFVAKMQEIYVVDNKAAEILDLRNDDDSVLGYVIKNSDFKKILLSRMTN
EEEEEHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCC
NPWITLIDHNQYQDVISHNDYSIIKFDDKHIKCSLLIICDGANSKVKSHYFANEIEKSYQ
CCEEEEEECCHHHHHHCCCCEEEEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHE
TALTFNIKHENPHENCAMEHFLPLGPFALLPLKNQYASSVIWSTSSDQAALIVNLPVEEV
EEEEEEEECCCCCCCHHHHHCCCCCCEEEEECCHHHHCEEEEECCCCCEEEEEECCHHHH
RFLTQRNAGNSLGKITIDSEISSFPLKARIANRYFHNKIVLIADTAHTIHPLAGQGLNQG
HHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHCCCCCCCHHHH
IKDIETLSMIVSNNGTLQEYQKLRQEDNFIMYKLTDELNNIFSNYAKNVRYLRQIGFKAI
HHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
NNFKPIKTLITGYAMGKR
CCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTKQIDTIILGCGLSGMLTALSFAEKGIKTTIFESQSVKSPEFFNDIRITALTPHSKNF
CCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCE
LFSIDIWAELEKFVAKMQEIYVVDNKAAEILDLRNDDDSVLGYVIKNSDFKKILLSRMTN
EEEEEHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCC
NPWITLIDHNQYQDVISHNDYSIIKFDDKHIKCSLLIICDGANSKVKSHYFANEIEKSYQ
CCEEEEEECCHHHHHHCCCCEEEEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHE
TALTFNIKHENPHENCAMEHFLPLGPFALLPLKNQYASSVIWSTSSDQAALIVNLPVEEV
EEEEEEEECCCCCCCHHHHHCCCCCCEEEEECCHHHHCEEEEECCCCCEEEEEECCHHHH
RFLTQRNAGNSLGKITIDSEISSFPLKARIANRYFHNKIVLIADTAHTIHPLAGQGLNQG
HHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHCCCCCCCHHHH
IKDIETLSMIVSNNGTLQEYQKLRQEDNFIMYKLTDELNNIFSNYAKNVRYLRQIGFKAI
HHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
NNFKPIKTLITGYAMGKR
CCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]