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The map label for this gene is ppdK [H]

Identifier: 157825797

GI number: 157825797

Start: 658545

End: 661181

Strand: Reverse

Name: ppdK [H]

Synonym: A1C_03640

Alternate gene names: 157825797

Gene position: 661181-658545 (Counterclockwise)

Preceding gene: 157825799

Following gene: 157825795

Centisome position: 53.71

GC content: 34.93

Gene sequence:

>2637_bases
ATGAAAAAATTAATTTATTATTTTGGCAGTAATGGTAGTGACGGAAACGCTAGCATGAAGAATGTCCTTGGTAACAAAGG
TGCCGGTCTTGCTGAAATGTCTAATCTAAAACTCCCTATTCCTGATGGTTTTACAATAACTACGGATCTATGTAATTACT
TTTATACCCACAATAATAACTTTCCTAAAAATTTTCAAAACGACTTAAAAAAGGCTATCACCAAACTTGAAATAACTACC
GGTAAAATTTTCGGCAGCACAAGCAATCCACTATTATTATCGGTTCGTTCGGGTTCTACGATTTCAATGCCTGGAATGAT
GGATACTATCCTTAATCTTGGCATGAATAACGAAGTGTGTAATGCTCTTGCGGATTCCTGTGGTGATAAACGATTTGCTC
TTGATAGTTACAAAAGATTTCTAGAAATGTATGGTTCAACGGTATTATCTATACCTAGTGATCTATTTGAACAAATTTAT
GAAAAGCACAAGATTCAAGCCGATATTCATAAAGATAGTGATATTACCGTAGAATTATTAGAAAAAATTATTGAAGATTT
TAAACGGTTGCATATTAAGTATACTAAGCAACTAATTAATGATCCTTATGAACAATTAGAATCGGCAATTAAAGCGGTGC
TGCATTCTTGGATGAGTAATAGAGCGGTTATATATAGAAAAATCAATAATATTTCGGAGTGTTTCGGTACGGCTATTAAT
ATCCAAGCAATGGTTTTCGGTAATTTAGGTAAAACTTCAGCTACGGGAGTTGCTTTTACTAGATCACCTGCTACAGGAGA
AAAAAAGCTCTTTGGAGAGTTTTTAATAAATGCTCAAGGTGAAGACATAGTATCTGGAACTAGAACACCTATGCCTATTA
TTGCCAATGGTTCAAATTCTATGCAAGCAATGATGCCGGAAGTGTTTAAGGAATTATCACAAATTGCGAGAAAGCTTGAA
GAACATTACTTAGATGTGCAGGATATAGAATTTACTATAGAAAATAATAAGCTATATATCTTACAAACACGTACTGCAAA
AAGAACAGCCATCGCTGCTATTAATATTGCCGTACAAATGGTAGAAGAAAAGCTTATTTCTAAAGAACAAGCCTTAATGC
GAATCGATCCGGAATCATTAAATCAATTATTGCATACAAGTATTGATTACAGTAAAGGGTTTACTTCTATTGCCGACGGG
CTTCCTGCGTCTCCAGGTGCTGCAACTGGTATTGCAGTATTCTCGCCTTATGATGCTGAAAAATTATCTCATCATCATAA
AGTAATTTTAGTACGTCATGATACAAGCCCCGAAGATATTAACGGAATGCATGTTTCTTCAGGTATTGTAACTATTAGAG
GCGGAATGACTTCACATGCCGCAGTTGTGGCAAGAGGTATGGGGAAGCCTTGTGTGTGCGGTACAAGTAATTTATCTATA
GATGAAAAAAAGCAAATATTAACAGCAGGTGATATAGTTATTAAACAAGGTGATATTATCACGATTGATGGAGGTAGCGG
TAAAATCTTCTTAGGTGAAATGCCGTTAATTCAACCTACTTTCAGTGAGGAATCAAAGTTAATTTTAGATTGGGCAGACG
AAATTAGTAGCTTAAAAGTTCGTGCAAATGCCGAAACGGTTAACGATGCTTTAGTATCTATAAAGTTGGGGGCTCAAGGT
ATAGGTTTGTGTCGTAGCGAACATATGTTTTTTGATAAAAATAAAATTCCTTTAGTAAGGGAAATGATTATTGCTCCTGA
TATAGAACGTAGAAAGCTTGCCGTGCAGAAATTATTGCCTCTGCAAACGGAGGATTTTAAAGCTTTATTCAGAGTGATGA
AAGATAAACCGGTAAATATTAGATTACTTGATCCGCCGTTACATGAGTTTTTACCTACTACTGAAGAAGATAAAAAGGAT
TTGGCAAACAGCTTAAATCTACCTTTGTCCATGATTAATCAACGTCTTCATGCTATGCATGAAGTAAATCCTATGCTCGG
TCACCGTGGATGTAGACTTGGTATATGTTCACCGGAAATTTACAAAATGCAAATTGAAGCAGTTTTTACGGCTATGTTTG
AGCTACATAAAAAAGAACATATAGAATGTAACTTAGAACTAATGATTCCTTTAATTAGCAATGTAGGTGAAATAAAAAAG
CTTAAAATGGATATTTATGAGGTAATAGAGGAATTAGAACAACGTTATAGATATAAATTTTCTTTTACGCTTGGTACTAT
GATAGAACTACCAAGGGCAGCGCTCGACAGTAAGAAAATAGCTAAGGAAGTTGACTATTTTAGTTTTGGTACTAATGATT
TAACTCAGACTACATATGGAATTTCTAGAGATGATATTGCATCTTTTCTTCCATATTATTTAGAAGAAAAGATTTTTGAA
TCCGATCCATTTACTACTCTAGATGAAGAAGGAGTAGGAGAATTAATTGAAATTGCTATAAAACGAGGGAAAAGCAGTAA
TGCTAATTTGAAACTCGGAGCTTGTGGGGAACATGCAGGAAACCCTGCTTCTATAGCATTTTTTCATAGAATGAATTTAA
AATATGTTTCTTGCTCTCCATACCGTATCCCTATAGCAAGAATCGCCGCAGCGCAAGCTAAGATTAAACACGGGTGA

Upstream 100 bases:

>100_bases
TATTCTTAAATTTAACATTGCTACTATTGTTTTCATACTGTATAAATATGTGTTTAATGCTTCTAAACACATAAATTGTT
AGCAAATTTTGATATATGAT

Downstream 100 bases:

>100_bases
ATAAAGGCGGTTGCACTCATGGTCAAGCCATGGGGGGTAACACAGTGGGTTTTATAGCTCCTCGCAATGACCAAAAATTT
ATTTACAAATTAATATGATA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 878; Mature: 878

Protein sequence:

>878_residues
MKKLIYYFGSNGSDGNASMKNVLGNKGAGLAEMSNLKLPIPDGFTITTDLCNYFYTHNNNFPKNFQNDLKKAITKLEITT
GKIFGSTSNPLLLSVRSGSTISMPGMMDTILNLGMNNEVCNALADSCGDKRFALDSYKRFLEMYGSTVLSIPSDLFEQIY
EKHKIQADIHKDSDITVELLEKIIEDFKRLHIKYTKQLINDPYEQLESAIKAVLHSWMSNRAVIYRKINNISECFGTAIN
IQAMVFGNLGKTSATGVAFTRSPATGEKKLFGEFLINAQGEDIVSGTRTPMPIIANGSNSMQAMMPEVFKELSQIARKLE
EHYLDVQDIEFTIENNKLYILQTRTAKRTAIAAINIAVQMVEEKLISKEQALMRIDPESLNQLLHTSIDYSKGFTSIADG
LPASPGAATGIAVFSPYDAEKLSHHHKVILVRHDTSPEDINGMHVSSGIVTIRGGMTSHAAVVARGMGKPCVCGTSNLSI
DEKKQILTAGDIVIKQGDIITIDGGSGKIFLGEMPLIQPTFSEESKLILDWADEISSLKVRANAETVNDALVSIKLGAQG
IGLCRSEHMFFDKNKIPLVREMIIAPDIERRKLAVQKLLPLQTEDFKALFRVMKDKPVNIRLLDPPLHEFLPTTEEDKKD
LANSLNLPLSMINQRLHAMHEVNPMLGHRGCRLGICSPEIYKMQIEAVFTAMFELHKKEHIECNLELMIPLISNVGEIKK
LKMDIYEVIEELEQRYRYKFSFTLGTMIELPRAALDSKKIAKEVDYFSFGTNDLTQTTYGISRDDIASFLPYYLEEKIFE
SDPFTTLDEEGVGELIEIAIKRGKSSNANLKLGACGEHAGNPASIAFFHRMNLKYVSCSPYRIPIARIAAAQAKIKHG

Sequences:

>Translated_878_residues
MKKLIYYFGSNGSDGNASMKNVLGNKGAGLAEMSNLKLPIPDGFTITTDLCNYFYTHNNNFPKNFQNDLKKAITKLEITT
GKIFGSTSNPLLLSVRSGSTISMPGMMDTILNLGMNNEVCNALADSCGDKRFALDSYKRFLEMYGSTVLSIPSDLFEQIY
EKHKIQADIHKDSDITVELLEKIIEDFKRLHIKYTKQLINDPYEQLESAIKAVLHSWMSNRAVIYRKINNISECFGTAIN
IQAMVFGNLGKTSATGVAFTRSPATGEKKLFGEFLINAQGEDIVSGTRTPMPIIANGSNSMQAMMPEVFKELSQIARKLE
EHYLDVQDIEFTIENNKLYILQTRTAKRTAIAAINIAVQMVEEKLISKEQALMRIDPESLNQLLHTSIDYSKGFTSIADG
LPASPGAATGIAVFSPYDAEKLSHHHKVILVRHDTSPEDINGMHVSSGIVTIRGGMTSHAAVVARGMGKPCVCGTSNLSI
DEKKQILTAGDIVIKQGDIITIDGGSGKIFLGEMPLIQPTFSEESKLILDWADEISSLKVRANAETVNDALVSIKLGAQG
IGLCRSEHMFFDKNKIPLVREMIIAPDIERRKLAVQKLLPLQTEDFKALFRVMKDKPVNIRLLDPPLHEFLPTTEEDKKD
LANSLNLPLSMINQRLHAMHEVNPMLGHRGCRLGICSPEIYKMQIEAVFTAMFELHKKEHIECNLELMIPLISNVGEIKK
LKMDIYEVIEELEQRYRYKFSFTLGTMIELPRAALDSKKIAKEVDYFSFGTNDLTQTTYGISRDDIASFLPYYLEEKIFE
SDPFTTLDEEGVGELIEIAIKRGKSSNANLKLGACGEHAGNPASIAFFHRMNLKYVSCSPYRIPIARIAAAQAKIKHG
>Mature_878_residues
MKKLIYYFGSNGSDGNASMKNVLGNKGAGLAEMSNLKLPIPDGFTITTDLCNYFYTHNNNFPKNFQNDLKKAITKLEITT
GKIFGSTSNPLLLSVRSGSTISMPGMMDTILNLGMNNEVCNALADSCGDKRFALDSYKRFLEMYGSTVLSIPSDLFEQIY
EKHKIQADIHKDSDITVELLEKIIEDFKRLHIKYTKQLINDPYEQLESAIKAVLHSWMSNRAVIYRKINNISECFGTAIN
IQAMVFGNLGKTSATGVAFTRSPATGEKKLFGEFLINAQGEDIVSGTRTPMPIIANGSNSMQAMMPEVFKELSQIARKLE
EHYLDVQDIEFTIENNKLYILQTRTAKRTAIAAINIAVQMVEEKLISKEQALMRIDPESLNQLLHTSIDYSKGFTSIADG
LPASPGAATGIAVFSPYDAEKLSHHHKVILVRHDTSPEDINGMHVSSGIVTIRGGMTSHAAVVARGMGKPCVCGTSNLSI
DEKKQILTAGDIVIKQGDIITIDGGSGKIFLGEMPLIQPTFSEESKLILDWADEISSLKVRANAETVNDALVSIKLGAQG
IGLCRSEHMFFDKNKIPLVREMIIAPDIERRKLAVQKLLPLQTEDFKALFRVMKDKPVNIRLLDPPLHEFLPTTEEDKKD
LANSLNLPLSMINQRLHAMHEVNPMLGHRGCRLGICSPEIYKMQIEAVFTAMFELHKKEHIECNLELMIPLISNVGEIKK
LKMDIYEVIEELEQRYRYKFSFTLGTMIELPRAALDSKKIAKEVDYFSFGTNDLTQTTYGISRDDIASFLPYYLEEKIFE
SDPFTTLDEEGVGELIEIAIKRGKSSNANLKLGACGEHAGNPASIAFFHRMNLKYVSCSPYRIPIARIAAAQAKIKHG

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=457, Percent_Identity=25.164113785558, Blast_Score=128, Evalue=2e-30,
Organism=Escherichia coli, GI1789193, Length=424, Percent_Identity=25, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788726, Length=455, Percent_Identity=23.956043956044, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1787994, Length=698, Percent_Identity=23.4957020057307, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI48994992, Length=355, Percent_Identity=25.0704225352113, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97777; Mature: 97777

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIYYFGSNGSDGNASMKNVLGNKGAGLAEMSNLKLPIPDGFTITTDLCNYFYTHNNN
CCEEEEEECCCCCCCCHHHHHHHCCCCCCCHHHCCCEECCCCCCEEHHHHHHHHEECCCC
FPKNFQNDLKKAITKLEITTGKIFGSTSNPLLLSVRSGSTISMPGMMDTILNLGMNNEVC
CCHHHHHHHHHHHHHEEEEECEEECCCCCCEEEEEECCCEEECCHHHHHHHHCCCCHHHH
NALADSCGDKRFALDSYKRFLEMYGSTVLSIPSDLFEQIYEKHKIQADIHKDSDITVELL
HHHHHHCCCCCEEHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCEEECCCCCCCHHHHH
EKIIEDFKRLHIKYTKQLINDPYEQLESAIKAVLHSWMSNRAVIYRKINNISECFGTAIN
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCEE
IQAMVFGNLGKTSATGVAFTRSPATGEKKLFGEFLINAQGEDIVSGTRTPMPIIANGSNS
EEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHCCCCCCCCEEECCCCH
MQAMMPEVFKELSQIARKLEEHYLDVQDIEFTIENNKLYILQTRTAKRTAIAAINIAVQM
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHH
VEEKLISKEQALMRIDPESLNQLLHTSIDYSKGFTSIADGLPASPGAATGIAVFSPYDAE
HHHHHHHHHHHHHEECHHHHHHHHHHHCCHHCCHHHHHCCCCCCCCCCCCEEEECCCCHH
KLSHHHKVILVRHDTSPEDINGMHVSSGIVTIRGGMTSHAAVVARGMGKPCVCGTSNLSI
HHCCCCEEEEEEECCCCCCCCCEEEECCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCC
DEKKQILTAGDIVIKQGDIITIDGGSGKIFLGEMPLIQPTFSEESKLILDWADEISSLKV
CHHHHHEEECCEEEECCCEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEEHHHHHHEEE
RANAETVNDALVSIKLGAQGIGLCRSEHMFFDKNKIPLVREMIIAPDIERRKLAVQKLLP
EECCHHHCCEEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCC
LQTEDFKALFRVMKDKPVNIRLLDPPLHEFLPTTEEDKKDLANSLNLPLSMINQRLHAMH
CCCHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
EVNPMLGHRGCRLGICSPEIYKMQIEAVFTAMFELHKKEHIECNLELMIPLISNVGEIKK
HCCHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEHEEEEHHHCCCHHHHH
LKMDIYEVIEELEQRYRYKFSFTLGTMIELPRAALDSKKIAKEVDYFSFGTNDLTQTTYG
HHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHCC
ISRDDIASFLPYYLEEKIFESDPFTTLDEEGVGELIEIAIKRGKSSNANLKLGACGEHAG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
NPASIAFFHRMNLKYVSCSPYRIPIARIAAAQAKIKHG
CCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLIYYFGSNGSDGNASMKNVLGNKGAGLAEMSNLKLPIPDGFTITTDLCNYFYTHNNN
CCEEEEEECCCCCCCCHHHHHHHCCCCCCCHHHCCCEECCCCCCEEHHHHHHHHEECCCC
FPKNFQNDLKKAITKLEITTGKIFGSTSNPLLLSVRSGSTISMPGMMDTILNLGMNNEVC
CCHHHHHHHHHHHHHEEEEECEEECCCCCCEEEEEECCCEEECCHHHHHHHHCCCCHHHH
NALADSCGDKRFALDSYKRFLEMYGSTVLSIPSDLFEQIYEKHKIQADIHKDSDITVELL
HHHHHHCCCCCEEHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCEEECCCCCCCHHHHH
EKIIEDFKRLHIKYTKQLINDPYEQLESAIKAVLHSWMSNRAVIYRKINNISECFGTAIN
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCEE
IQAMVFGNLGKTSATGVAFTRSPATGEKKLFGEFLINAQGEDIVSGTRTPMPIIANGSNS
EEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHCCCCCCCCEEECCCCH
MQAMMPEVFKELSQIARKLEEHYLDVQDIEFTIENNKLYILQTRTAKRTAIAAINIAVQM
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHH
VEEKLISKEQALMRIDPESLNQLLHTSIDYSKGFTSIADGLPASPGAATGIAVFSPYDAE
HHHHHHHHHHHHHEECHHHHHHHHHHHCCHHCCHHHHHCCCCCCCCCCCCEEEECCCCHH
KLSHHHKVILVRHDTSPEDINGMHVSSGIVTIRGGMTSHAAVVARGMGKPCVCGTSNLSI
HHCCCCEEEEEEECCCCCCCCCEEEECCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCC
DEKKQILTAGDIVIKQGDIITIDGGSGKIFLGEMPLIQPTFSEESKLILDWADEISSLKV
CHHHHHEEECCEEEECCCEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEEHHHHHHEEE
RANAETVNDALVSIKLGAQGIGLCRSEHMFFDKNKIPLVREMIIAPDIERRKLAVQKLLP
EECCHHHCCEEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCHHHHHHHHHHCC
LQTEDFKALFRVMKDKPVNIRLLDPPLHEFLPTTEEDKKDLANSLNLPLSMINQRLHAMH
CCCHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
EVNPMLGHRGCRLGICSPEIYKMQIEAVFTAMFELHKKEHIECNLELMIPLISNVGEIKK
HCCHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEHEEEEHHHCCCHHHHH
LKMDIYEVIEELEQRYRYKFSFTLGTMIELPRAALDSKKIAKEVDYFSFGTNDLTQTTYG
HHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHCC
ISRDDIASFLPYYLEEKIFESDPFTTLDEEGVGELIEIAIKRGKSSNANLKLGACGEHAG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
NPASIAFFHRMNLKYVSCSPYRIPIARIAAAQAKIKHG
CCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]