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The map label for this gene is def2 [H]

Identifier: 157825208

GI number: 157825208

Start: 68372

End: 68674

Strand: Reverse

Name: def2 [H]

Synonym: A1C_00435

Alternate gene names: 157825208

Gene position: 68674-68372 (Counterclockwise)

Preceding gene: 157825220

Following gene: 157825205

Centisome position: 5.58

GC content: 30.69

Gene sequence:

>303_bases
ATGCCTAAAACTATATGGATTAATCCATCATATAAACAGGTAGATATTGATAAACATGAAGATTATGAAGGATTTTTTTC
GGTTGAAAATGCTACAGGTCCAGTTGCAAGATTCAAAAAGATTCATTGTCACGCATATGATATAAATGGACATCAAATCC
AAGGCATCACAGAAGGATTTTTAGCAAGAGTTATCCAGCATGAAATCGATCATCTAAACGGAAAAGTATTTTTAGATTAT
GTAGCTCCCGAAAAAATAATGACAAAAGAAAAATATTTAGAAAAACGTAAAAAAGCTATGTAG

Upstream 100 bases:

>100_bases
GCTTGCTGCACCGCAAATAGATATCTCAAAATATATAATAATATTTGCAGTAAATGAAAATGAAGAGTTAAAAAAATGAC
GTCCTGATCTTAAGGATACA

Downstream 100 bases:

>100_bases
CAGGAAAATATAAAATCTTAGATTCATCCAAATTTCAAATATCATTCTAGCTAAATTTTAGTATGTCATGCCTCACTAAA
AGCTGCGTTATTGTATGAAA

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 100; Mature: 99

Protein sequence:

>100_residues
MPKTIWINPSYKQVDIDKHEDYEGFFSVENATGPVARFKKIHCHAYDINGHQIQGITEGFLARVIQHEIDHLNGKVFLDY
VAPEKIMTKEKYLEKRKKAM

Sequences:

>Translated_100_residues
MPKTIWINPSYKQVDIDKHEDYEGFFSVENATGPVARFKKIHCHAYDINGHQIQGITEGFLARVIQHEIDHLNGKVFLDY
VAPEKIMTKEKYLEKRKKAM
>Mature_99_residues
PKTIWINPSYKQVDIDKHEDYEGFFSVENATGPVARFKKIHCHAYDINGHQIQGITEGFLARVIQHEIDHLNGKVFLDYV
APEKIMTKEKYLEKRKKAM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 11690; Mature: 11558

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTIWINPSYKQVDIDKHEDYEGFFSVENATGPVARFKKIHCHAYDINGHQIQGITEGF
CCCEEEECCCCCEEECCCCCCCCCEEEECCCCCCHHHHHEEEEEEEECCCCEECCHHHHH
LARVIQHEIDHLNGKVFLDYVAPEKIMTKEKYLEKRKKAM
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKTIWINPSYKQVDIDKHEDYEGFFSVENATGPVARFKKIHCHAYDINGHQIQGITEGF
CCEEEECCCCCEEECCCCCCCCCEEEECCCCCCHHHHHEEEEEEEECCCCEECCHHHHH
LARVIQHEIDHLNGKVFLDYVAPEKIMTKEKYLEKRKKAM
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]