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The map label for this gene is cca [H]

Identifier: 157825180

GI number: 157825180

Start: 44786

End: 45943

Strand: Direct

Name: cca [H]

Synonym: A1C_00295

Alternate gene names: 157825180

Gene position: 44786-45943 (Clockwise)

Preceding gene: 157825179

Following gene: 157825181

Centisome position: 3.64

GC content: 29.62

Gene sequence:

>1158_bases
ATGCAAGTTATACATACAACTTTAAAAATCCCTTCAAAAGGATATAAAAAAATCTTGAGCCTTTTAAATAAAAAAGGACA
AGCAAGACTTATTGGAGGCTGCGTACGAGATGCGTTACTTGAAAAAAGCAGTTATGATATCGATATAGCTACTAATTTAA
TACCGGATGAAGTAACAAATATTTTATTGAAAGCTAATATCAAAACTATCCAGACCGGTTTAAAATTTGGTACGATTACG
GCTATTTTAAATAATGAGCAATTTGAGATTACAACTCTTAGAAAAGATATCGAATGCAACGGTAGACATGCCAAAGTAGT
ATTCACCGACGATTTTGCCGAAGATGCTGCAAGACGAGATTTTACTATTAATGCTTTAAGCTATTGCCCATTTAAGAACG
AGATATATGATTATTTTGAAGGCTTTAAGGATTTGCGGCAGGAAAAGGTTGTATTTATAGGGGAAGCTCTAGATAGAATT
AAAGAAGATTACTTACGAATCCTACGCTTTTTTCGGTTTTCTAGCTATTATACGAATCAGCTTGATGACGGCATCTTTAA
AGCATGTAAAGCTTTAAAAGACGGCTTAAAAACTTTATCACGAGAGCGAATTAAAAGCGAAATGGATAAAATTATCGTTT
CAAAAAGAGCGGAACAAATTTTAAAAGCTATGTTTGAAATAGGAATATTAGAACTAATATTCTCTATCCAAAATTATGAA
ATAAAATTTTTTGAGCAATCTAAAGCATTAGAATTAGCAACTAATTATGCTTTATTACTATATAATTTAAAAAACATAAA
TTCAAAGCTTCTTCTCGATTGGAAATTCACAAAACAGGAAGTAATACAAATATTATCAATAATAAACTTTTTGAATAACG
CTGAATGTGATATAAAAAAAATTTGGTTTGAAAAGAAGAACTATAAGGAATATTTATTGGCAGCTAGCATAATCGGTAAA
TTAAACCATTCACAAGTAAAAGAATTTATCCATAAATATGATCCATTATTACGTCCGAAATTCCAAGTAAACGGTAATGA
CTTATTAAATATGAATATAGAAAAAAAAGAGATAGGAGCAAAACTAGAATATCTAAAAAATTTTTGGATAGACCAGGATT
TCAAGCCTAGTAAATCTGAGTTGTTGGAAAAGTTATGA

Upstream 100 bases:

>100_bases
AATACTGGATTCCCGCCTACGCGGGAATGACATAAAGGAACGAGTTGATAACCCTAGAGTCTTTTTAAAAACTACCAGAT
ACTATAGGTAAAATCAAATA

Downstream 100 bases:

>100_bases
AGATTAAGATTTATAATATACTTCTAATGTCATCATGCGACTTGATCGCGGGATCCAAAAAAATGGATACCGTGGTCAAG
CCACGGTATAACACCGGAAA

Product: Poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MQVIHTTLKIPSKGYKKILSLLNKKGQARLIGGCVRDALLEKSSYDIDIATNLIPDEVTNILLKANIKTIQTGLKFGTIT
AILNNEQFEITTLRKDIECNGRHAKVVFTDDFAEDAARRDFTINALSYCPFKNEIYDYFEGFKDLRQEKVVFIGEALDRI
KEDYLRILRFFRFSSYYTNQLDDGIFKACKALKDGLKTLSRERIKSEMDKIIVSKRAEQILKAMFEIGILELIFSIQNYE
IKFFEQSKALELATNYALLLYNLKNINSKLLLDWKFTKQEVIQILSIINFLNNAECDIKKIWFEKKNYKEYLLAASIIGK
LNHSQVKEFIHKYDPLLRPKFQVNGNDLLNMNIEKKEIGAKLEYLKNFWIDQDFKPSKSELLEKL

Sequences:

>Translated_385_residues
MQVIHTTLKIPSKGYKKILSLLNKKGQARLIGGCVRDALLEKSSYDIDIATNLIPDEVTNILLKANIKTIQTGLKFGTIT
AILNNEQFEITTLRKDIECNGRHAKVVFTDDFAEDAARRDFTINALSYCPFKNEIYDYFEGFKDLRQEKVVFIGEALDRI
KEDYLRILRFFRFSSYYTNQLDDGIFKACKALKDGLKTLSRERIKSEMDKIIVSKRAEQILKAMFEIGILELIFSIQNYE
IKFFEQSKALELATNYALLLYNLKNINSKLLLDWKFTKQEVIQILSIINFLNNAECDIKKIWFEKKNYKEYLLAASIIGK
LNHSQVKEFIHKYDPLLRPKFQVNGNDLLNMNIEKKEIGAKLEYLKNFWIDQDFKPSKSELLEKL
>Mature_385_residues
MQVIHTTLKIPSKGYKKILSLLNKKGQARLIGGCVRDALLEKSSYDIDIATNLIPDEVTNILLKANIKTIQTGLKFGTIT
AILNNEQFEITTLRKDIECNGRHAKVVFTDDFAEDAARRDFTINALSYCPFKNEIYDYFEGFKDLRQEKVVFIGEALDRI
KEDYLRILRFFRFSSYYTNQLDDGIFKACKALKDGLKTLSRERIKSEMDKIIVSKRAEQILKAMFEIGILELIFSIQNYE
IKFFEQSKALELATNYALLLYNLKNINSKLLLDWKFTKQEVIQILSIINFLNNAECDIKKIWFEKKNYKEYLLAASIIGK
LNHSQVKEFIHKYDPLLRPKFQVNGNDLLNMNIEKKEIGAKLEYLKNFWIDQDFKPSKSELLEKL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=391, Percent_Identity=33.7595907928389, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI87081691, Length=232, Percent_Identity=28.8793103448276, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=420, Percent_Identity=31.4285714285714, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI281360112, Length=381, Percent_Identity=34.3832020997375, Blast_Score=178, Evalue=6e-45,
Organism=Drosophila melanogaster, GI21357337, Length=381, Percent_Identity=34.3832020997375, Blast_Score=178, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 44885; Mature: 44885

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVIHTTLKIPSKGYKKILSLLNKKGQARLIGGCVRDALLEKSSYDIDIATNLIPDEVTN
CCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHH
ILLKANIKTIQTGLKFGTITAILNNEQFEITTLRKDIECNGRHAKVVFTDDFAEDAARRD
HHHHCCHHHHHHCCCHHEEEEEECCCCEEEEEEHHCCCCCCCEEEEEEECCHHHHHHHCC
FTINALSYCPFKNEIYDYFEGFKDLRQEKVVFIGEALDRIKEDYLRILRFFRFSSYYTNQ
EEEEHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDGIFKACKALKDGLKTLSRERIKSEMDKIIVSKRAEQILKAMFEIGILELIFSIQNYE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IKFFEQSKALELATNYALLLYNLKNINSKLLLDWKFTKQEVIQILSIINFLNNAECDIKK
EEEEECCHHHHHHHHHEEEEEEHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCHHH
IWFEKKNYKEYLLAASIIGKLNHSQVKEFIHKYDPLLRPKFQVNGNDLLNMNIEKKEIGA
HHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCCEEECCCHHHHHHH
KLEYLKNFWIDQDFKPSKSELLEKL
HHHHHHHHCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MQVIHTTLKIPSKGYKKILSLLNKKGQARLIGGCVRDALLEKSSYDIDIATNLIPDEVTN
CCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHH
ILLKANIKTIQTGLKFGTITAILNNEQFEITTLRKDIECNGRHAKVVFTDDFAEDAARRD
HHHHCCHHHHHHCCCHHEEEEEECCCCEEEEEEHHCCCCCCCEEEEEEECCHHHHHHHCC
FTINALSYCPFKNEIYDYFEGFKDLRQEKVVFIGEALDRIKEDYLRILRFFRFSSYYTNQ
EEEEHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDGIFKACKALKDGLKTLSRERIKSEMDKIIVSKRAEQILKAMFEIGILELIFSIQNYE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IKFFEQSKALELATNYALLLYNLKNINSKLLLDWKFTKQEVIQILSIINFLNNAECDIKK
EEEEECCHHHHHHHHHEEEEEEHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCHHH
IWFEKKNYKEYLLAASIIGKLNHSQVKEFIHKYDPLLRPKFQVNGNDLLNMNIEKKEIGA
HHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCCEEECCCHHHHHHH
KLEYLKNFWIDQDFKPSKSELLEKL
HHHHHHHHCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA