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The map label for this gene is gidB

Identifier: 157373140

GI number: 157373140

Start: 5448157

End: 5448777

Strand: Direct

Name: gidB

Synonym: Spro_4909

Alternate gene names: 157373140

Gene position: 5448157-5448777 (Clockwise)

Preceding gene: 157373139

Following gene: NA

Centisome position: 99.99

GC content: 55.56

Gene sequence:

>621_bases
GTGCAGAAAAAATTAGATTCGTTGCTCAAAGCGGCAGGCATTACGCTTCCCGATCGGCAAAAACAGCAGTTGCTGGGCTA
TGTAGGCATGCTGGATAAGTGGAACAAGGCGTATAACCTCACTTCGGTGCGCGATCCGCAGCAAATGCTGGTTCGCCATA
TTCTCGACAGCATCGTAGTCAACCCGCATCTGCAGGGTAAGCGTTTTATCGACGTGGGCACCGGTCCCGGTTTGCCAGGC
ATTCCGCTGGCCATTGTTCGCCCCGATTCCCATTTCACCTTGCTCGACAGCCTGGGCAAGCGTGTACGCTTCCTGCGTCA
GGTGCAGCATGAACTGGGTTTGACCAACGTTGAACCGGTTCAGAGCCGCGTTGAGGAGTTTCCTGCGGAGCCGCCGTTTG
ACGGCGTCATCAGCCGCGCCTTCGCCTCATTGCAGGATATGCTGTCCTGGTGTCATCATTTGCCGGCAAAAGGGCAGGGG
CGTTTCTACGCGCTGAAAGGAGTACGCCCAGACGAAGAGCTTACGCAGTTGCCGCCGGGCATTGAGTTGGAATCAGTCGT
ACGTCTGCAGGTACCGGAACTCGAAGGTGAACGTCATCTGGTTATCCTTAAGGCAAACTGA

Upstream 100 bases:

>100_bases
CAAACGGCCGCTGACATCGTTTCTGGCCTGAATTATTATGGTCACCTTCTTGGGCAGCCCCTACCTGGCTTGCCGTTGTG
CGCCAGTCTAAGGACTCATT

Downstream 100 bases:

>100_bases
GTTTGTTTTTGATCAAGTAAGCGCGAATTAAAAGATAGTGCCCATGGTTAAAGTAAGCAGACTAATCAATGGGTGGTTTT
TTTAGTTAATTTACTTTTTA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MQKKLDSLLKAAGITLPDRQKQQLLGYVGMLDKWNKAYNLTSVRDPQQMLVRHILDSIVVNPHLQGKRFIDVGTGPGLPG
IPLAIVRPDSHFTLLDSLGKRVRFLRQVQHELGLTNVEPVQSRVEEFPAEPPFDGVISRAFASLQDMLSWCHHLPAKGQG
RFYALKGVRPDEELTQLPPGIELESVVRLQVPELEGERHLVILKAN

Sequences:

>Translated_206_residues
MQKKLDSLLKAAGITLPDRQKQQLLGYVGMLDKWNKAYNLTSVRDPQQMLVRHILDSIVVNPHLQGKRFIDVGTGPGLPG
IPLAIVRPDSHFTLLDSLGKRVRFLRQVQHELGLTNVEPVQSRVEEFPAEPPFDGVISRAFASLQDMLSWCHHLPAKGQG
RFYALKGVRPDEELTQLPPGIELESVVRLQVPELEGERHLVILKAN
>Mature_206_residues
MQKKLDSLLKAAGITLPDRQKQQLLGYVGMLDKWNKAYNLTSVRDPQQMLVRHILDSIVVNPHLQGKRFIDVGTGPGLPG
IPLAIVRPDSHFTLLDSLGKRVRFLRQVQHELGLTNVEPVQSRVEEFPAEPPFDGVISRAFASLQDMLSWCHHLPAKGQG
RFYALKGVRPDEELTQLPPGIELESVVRLQVPELEGERHLVILKAN

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=206, Percent_Identity=77.1844660194175, Blast_Score=329, Evalue=9e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_SERP5 (A8GLL1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001481129.1
- ProteinModelPortal:   A8GLL1
- SMR:   A8GLL1
- STRING:   A8GLL1
- GeneID:   5603165
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4909
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   SPRO399741:SPRO_4909-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23127; Mature: 23127

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKLDSLLKAAGITLPDRQKQQLLGYVGMLDKWNKAYNLTSVRDPQQMLVRHILDSIVV
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
NPHLQGKRFIDVGTGPGLPGIPLAIVRPDSHFTLLDSLGKRVRFLRQVQHELGLTNVEPV
CCCCCCCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QSRVEEFPAEPPFDGVISRAFASLQDMLSWCHHLPAKGQGRFYALKGVRPDEELTQLPPG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHCCCC
IELESVVRLQVPELEGERHLVILKAN
CCHHHHHEEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MQKKLDSLLKAAGITLPDRQKQQLLGYVGMLDKWNKAYNLTSVRDPQQMLVRHILDSIVV
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
NPHLQGKRFIDVGTGPGLPGIPLAIVRPDSHFTLLDSLGKRVRFLRQVQHELGLTNVEPV
CCCCCCCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QSRVEEFPAEPPFDGVISRAFASLQDMLSWCHHLPAKGQGRFYALKGVRPDEELTQLPPG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHCCCC
IELESVVRLQVPELEGERHLVILKAN
CCHHHHHEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA