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The map label for this gene is mnmG

Identifier: 157373139

GI number: 157373139

Start: 5446130

End: 5448019

Strand: Direct

Name: mnmG

Synonym: Spro_4908

Alternate gene names: 157373139

Gene position: 5446130-5448019 (Clockwise)

Preceding gene: 157373138

Following gene: 157373140

Centisome position: 99.95

GC content: 55.87

Gene sequence:

>1890_bases
ATGTTTTATCCAGAGCAATTTGACGTCATCATCATCGGTGGTGGCCATGCCGGTACCGAAGCCGCTATGGCTGCGGCACG
TATGGGACGTCAGACTTTATTACTGACTCACAATATCGATACGCTGGGGCAAATGTCTTGCAACCCGGCGATTGGCGGTA
TTGGTAAGGGGCATCTGGTTAAGGAAATTGATGCACTTGGCGGCCTGATGGCCACGGCGATCGACCATGCCGGCATCCAG
TTTAGGATACTAAACGCCAGCAAAGGCCCTGCGGTACGAGCCACCCGGGCTCAGGCTGACCGAGTACTGTATCGTCAAGC
GGTACGTACTGCCCTGGAGAACCAGCCCAATCTGATGATCTTCCAGCAACCGGTCGAAGATCTGATCGTGGAAAACGATC
GCGTTGTCGGTGCCGTGACCCAAATGGGGCTGAAATTCCGCGCCAAGGCGGTAGTACTGACCGTAGGTACCTTCCTGGAC
GGCAAGATCCACATTGGTCTGGAAAACTACAGCGGTGGCCGTGCCGGAGATCCGCCATCGATCTCACTGTCACAGCGACT
GCGTGAATTGCCTTTGCGTGTTAACCGACTGAAAACCGGTACACCGCCGCGTATCGATGCCCGCAGCATAGATTTCAGCG
TATTGGCACCTCAACACGGTGATACACCGATCCCGGTGTTCTCGTTCATGGGCAACGCTGGGCAACACCCTGAACAAATG
GCGTGTTACATCACCTACACCAATGAAAAAACCCATGATGTGATCCGTAATAACCTCGATCGCAGCCCGATGTATGCCGG
GATCATCGAAGGGATCGGCCCGCGTTACTGCCCGTCGATCGAAGACAAGGTGATGCGTTTTGCCGATCGTAACGCGCACC
AGATCTTCCTTGAGCCGGAAGGCCTGACCAGTAATGAAATTTATCCAAACGGCATCTCCACCAGCCTGCCGTTTGACGTA
CAGATGCAGATCGTCCGTTCGATGGAAGGGATGCAAAATGCCCGGATCATTCGACCAGGCTATGCCATCGAGTATGATTT
CTTCGATCCTCGCGATCTGAAGCCGACGCTGGAAAGCAAATTTATTCAGGGCCTGTTCTTCGCCGGCCAGATCAACGGCA
CCACCGGTTACGAAGAAGCTGCAGCGCAAGGCCTGTTAGCCGGCCTGAATGCCGGGCGCTACGCCAATGAAGACGAAGGC
TGGTCACCACGCCGCGATCAGGCTTATCTGGGCGTGCTGGTGGACGACCTCAGCACCCTGGGCACCAAAGAGCCGTACCG
CATGTTCACATCACGTGCCGAATACCGCCTGATGTTACGTGAAGACAATGCCGATCTGCGCCTGACGGAAAAAGGCCGCG
AACTGGGCCTGGTGGACGATGCCCGCTGGGCACGCTTTAGCGAAAAACTCGAGCACATCGAACAGGAGCGTCAGCGCCTG
CGCGACATCTGGATGCATCCGCACGCCGAAAACGTCGAGCAGGTGAACGCGCTGCTGAAAGCACCGCTGTCGCGTGAAGC
CAACGGTGAAGAACTGCTTCGCCGCCCGGAAATGGATTACACCCAATTGACCTCCGTCGCGGCATTTGCGCCGCCGCTGG
CCGATACTCAGGCAGCCGAGCAGGTTGAGATCCAGGTAAAATACGAAGGTTATATTGCTCGCCAGCAGGAAGAGATCGAA
AAACAGCAGCGCAATGAGAACACCGTGCTGCCGTTGGATCTCGATTACCAGCAGGTTTCTGGCCTGTCGAATGAAGTGAT
CGCCAAGTTGAATGACCACAAGCCGAATTCGATCGGCCAGGCTTCACGCATTTCTGGCATCACGCCGGCGGCGATCTCAA
TTCTACTGATTTGGCTGAAAAAGCAGGGGCTGCTGCGCCGCAGCGCATAA

Upstream 100 bases:

>100_bases
CTCAAGGATCCTGACCACTTGGTCGATCGACTAAACTTGAGTAGAATCCACCACCCCAGGGCAAACAACGATCGTTCGCA
ATACGACGAGGTGCAGTTCC

Downstream 100 bases:

>100_bases
ATCCTGTTTTTGGGCGGCCGTTTGATTATTTGATCGGCAAACGGCCGCTGACATCGTTTCTGGCCTGAATTATTATGGTC
ACCTTCTTGGGCAGCCCCTA

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYPEQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGLMATAIDHAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLENYSGGRAGDPPSISLSQRLRELPLRVNRLKTGTPPRIDARSIDFSVLAPQHGDTPIPVFSFMGNAGQHPEQM
ACYITYTNEKTHDVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMEGMQNARIIRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRYANEDEG
WSPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEKGRELGLVDDARWARFSEKLEHIEQERQRL
RDIWMHPHAENVEQVNALLKAPLSREANGEELLRRPEMDYTQLTSVAAFAPPLADTQAAEQVEIQVKYEGYIARQQEEIE
KQQRNENTVLPLDLDYQQVSGLSNEVIAKLNDHKPNSIGQASRISGITPAAISILLIWLKKQGLLRRSA

Sequences:

>Translated_629_residues
MFYPEQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGLMATAIDHAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLENYSGGRAGDPPSISLSQRLRELPLRVNRLKTGTPPRIDARSIDFSVLAPQHGDTPIPVFSFMGNAGQHPEQM
ACYITYTNEKTHDVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMEGMQNARIIRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRYANEDEG
WSPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEKGRELGLVDDARWARFSEKLEHIEQERQRL
RDIWMHPHAENVEQVNALLKAPLSREANGEELLRRPEMDYTQLTSVAAFAPPLADTQAAEQVEIQVKYEGYIARQQEEIE
KQQRNENTVLPLDLDYQQVSGLSNEVIAKLNDHKPNSIGQASRISGITPAAISILLIWLKKQGLLRRSA
>Mature_629_residues
MFYPEQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGLMATAIDHAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLENYSGGRAGDPPSISLSQRLRELPLRVNRLKTGTPPRIDARSIDFSVLAPQHGDTPIPVFSFMGNAGQHPEQM
ACYITYTNEKTHDVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMEGMQNARIIRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRYANEDEG
WSPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEKGRELGLVDDARWARFSEKLEHIEQERQRL
RDIWMHPHAENVEQVNALLKAPLSREANGEELLRRPEMDYTQLTSVAAFAPPLADTQAAEQVEIQVKYEGYIARQQEEIE
KQQRNENTVLPLDLDYQQVSGLSNEVIAKLNDHKPNSIGQASRISGITPAAISILLIWLKKQGLLRRSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=633, Percent_Identity=46.2875197472354, Blast_Score=515, Evalue=1e-146,
Organism=Homo sapiens, GI19882217, Length=658, Percent_Identity=44.5288753799392, Blast_Score=499, Evalue=1e-141,
Organism=Homo sapiens, GI183227703, Length=673, Percent_Identity=43.5364041604755, Blast_Score=495, Evalue=1e-140,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=87.758346581876, Blast_Score=1137, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=639, Percent_Identity=41.1580594679186, Blast_Score=473, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321202, Length=622, Percent_Identity=45.6591639871383, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=46.2264150943396, Blast_Score=527, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_SERP5 (A8GLL0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001481128.1
- ProteinModelPortal:   A8GLL0
- SMR:   A8GLL0
- STRING:   A8GLL0
- GeneID:   5603164
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4908
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   SPRO399741:SPRO_4908-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR000048
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70044; Mature: 70044

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2; PS50096 IQ

Important sites: BINDING 125-125 BINDING 180-180 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYPEQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGLMATAIDHAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLENYSGGRAGDPPS
ECCCHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEECEECCEEEEEEECCCCCCCCCCCC
ISLSQRLRELPLRVNRLKTGTPPRIDARSIDFSVLAPQHGDTPIPVFSFMGNAGQHPEQM
CCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCE
ACYITYTNEKTHDVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPE
EEEEEECCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMEGMQNARIIRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRYANEDEGWSPRRDQAYLGVLVDDLSTL
HHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GTKEPYRMFTSRAEYRLMLREDNADLRLTEKGRELGLVDDARWARFSEKLEHIEQERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHH
RDIWMHPHAENVEQVNALLKAPLSREANGEELLRRPEMDYTQLTSVAAFAPPLADTQAAE
HHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
QVEIQVKYEGYIARQQEEIEKQQRNENTVLPLDLDYQQVSGLSNEVIAKLNDHKPNSIGQ
EEEEEEEECCEEHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGITPAAISILLIWLKKQGLLRRSA
HHHCCCCCHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MFYPEQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGLMATAIDHAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLENYSGGRAGDPPS
ECCCHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEECEECCEEEEEEECCCCCCCCCCCC
ISLSQRLRELPLRVNRLKTGTPPRIDARSIDFSVLAPQHGDTPIPVFSFMGNAGQHPEQM
CCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCE
ACYITYTNEKTHDVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNAHQIFLEPE
EEEEEECCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMEGMQNARIIRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRYANEDEGWSPRRDQAYLGVLVDDLSTL
HHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GTKEPYRMFTSRAEYRLMLREDNADLRLTEKGRELGLVDDARWARFSEKLEHIEQERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHH
RDIWMHPHAENVEQVNALLKAPLSREANGEELLRRPEMDYTQLTSVAAFAPPLADTQAAE
HHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
QVEIQVKYEGYIARQQEEIEKQQRNENTVLPLDLDYQQVSGLSNEVIAKLNDHKPNSIGQ
EEEEEEEECCEEHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGITPAAISILLIWLKKQGLLRRSA
HHHCCCCCHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA