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The map label for this gene is spoT [H]

Identifier: 157373100

GI number: 157373100

Start: 5391421

End: 5393532

Strand: Direct

Name: spoT [H]

Synonym: Spro_4869

Alternate gene names: 157373100

Gene position: 5391421-5393532 (Clockwise)

Preceding gene: 157373099

Following gene: 157373101

Centisome position: 98.95

GC content: 54.69

Gene sequence:

>2112_bases
TTGTACCTGTTTGAAAGCCTGAATCTGCTGATTCAACGTTACCTGCCTGAGGAGCAGATTAAGCGCCTCAAACAGGCATA
CCTCGTTGCACGTGATGCTCACGAGGGACAGACACGCTCCAGCGGTGAGCCCTACATTACTCACCCGGTTGCCGTGGCCT
GCATTCTGGCGGAAATGCGTCTCGATCATGAGACGCTGATGGCAGCACTGCTGCACGACGTCATCGAAGATACCCCGGCC
ACCTACCAGGATATGGAACAGCTATTCGGCAAAAGCGTTGCCGAACTGGTTGAAGGCGTATCCAAGCTCGACAAGCTGAA
GTTCCAGGATAAAAAAGAAGCTCAGGCGGAAAACTTCCGCAAGATGATCATGGCGATGGTGCAGGATATCCGCGTCGTGC
TGATCAAGCTGGCCGACCGTACGCACAACATGCGCACTCTCGGTTCGCTGCGTCCTGACAAACGGCGGCGTATTGCACGT
GAAACCCTGGAAATCTACAGCCCCCTGGCCCATCGCCTGGGTATTCACCACCTGAAAACCGAGCTGGAAGAGCTGGGTTT
TGAGGCGTTGTACCCGAACCGCTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCACGCGGTAACCGTAAAGAGATGATCC
AGAAGATTCTCTCCGAGATCGAAGGGCGACTGACCGAGGCCGGCATTCCCTGCCGCGTCAGCGGACGGGAAAAACATCTG
TATTCCATCTACCTCAAGATGCACCTGAAAGAACAGCGTTTCCATTCGATTATGGATATCTACGCGTTCCGGGTGATCGT
GAAGGAAGTCGACACCTGCTACCGCGTGCTGGGCCAGGCTCACAGCCTGTACAAACCGCGTCCGGGCAGGGTCAAAGACT
ATATCGCCATTCCCAAGGCCAACGGCTATCAATCGCTGCACACCTCACTGATCGGCCCACATGGCGTACCGGTTGAAGTG
CAGATCCGTACCGAAGATATGGATCAGATGGCCGAAATGGGGGTCGCCGCGCACTGGGCTTATAAAGAAAAAGAACAGGG
CGAAACCGGCACCACCGCACAAATCCGCGCTCAGCGGTGGATGCAGAGCTTGCTGGAGCTGCAACAAAGCGCCGGCAGTT
CGTTTGAATTTATCGAGAGCGTAAAATCCGATCTGTTCCCGGATGAGATTTACGTTTTCACCCCGGAAGGCCGCATTGTT
GAATTGCCTGCGGGCGCAACGCCGGTCGACTTCGCCTACGCGGTGCACACCGATATCGGCCACGCCTGCGTTGGCGCACG
TGTTGATCGCCAGCCATACCCGCTGTCACAGTCCTTGACCAGCGGGCAAACGGTCGAAATCATCACGGCTCCTGGTGCAC
GGCCTAACGCCGCCTGGCTGAACTTTGTCGTCAGCTCAAAAGCGCGCGCAAAAATCCGCCAAATGCTGAAAAACCTCAAG
CGCGATGATTCAGTCGGCCTCGGCCGCCGCTTGTTAAATCATGCATTGGGCGGCAGTCGCAAACTGGCAGAGGTTCCGGC
AGAAAATATCCAACACGAGTTGGATCGCATGAAACTGGCGACGCTGGACGACCTGTTGGCTGAAATCGGCTTGGGCAATG
CCATGAGCGTGGTGGTAGCGAAAAACCTGCAAGGTGACCAGTCTAATCTGGGGGCCTCCTCTGGGGTTCGTAATCTGGCG
ATCAAGGGCTCAGACGGCGTGCTGATCACCTTCGCTAAATGCTGCCGACCTATTCCAGGCGATCCGATCATCGCCCACGT
CAGCCCAGGCAAAGGCCTGGTGATCCACCATGAGTCCTGCCGAAATATCCGCGGCTACCAGAAAGAACCTGAAAAATTCA
TGGCGGTAGAGTGGGATCAAGACATCGAACAGGAATTCATCGCCGAGATCAAAGTGGACATGTTTAATCATCAGGGGGCA
CTGGCCAACCTGACGGCAGCCATCAATGCGGCTGAGTCCAATATTCAAAGCCTGAATACCGAAGAAAAAGACGGCCGGGT
TTATAGTGCATTTATCCGTTTGACCACCCGCGATCGCATCCATCTGGCAAATATTATGCGTAAAATCCGTATCATGCCGG
ATGTGATTAAAGTTAACCGTAACCGAAATTAG

Upstream 100 bases:

>100_bases
GTTCGTGATCGTCAGGAGCAGCAAGAGCAGGAAGCCGCAGAGATTCAAGCGGTTACCGCCATTGCTGAAGGTCGTCGTTA
ATTAGACTGCGAGTCTGCCT

Downstream 100 bases:

>100_bases
CGCTTATGAGCCCTGAACGCTATGCGCGGATTTGTGAAATGCTGGCCACCCGGCAGCCGGATTTAACCGTCTGCCTGGAA
CAGGTTCATAAACCGCATAA

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMRLDHETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFQDKKEAQAENFRKMIMAMVQDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHLKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLTEAGIPCRVSGREKHL
YSIYLKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKANGYQSLHTSLIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEKEQGETGTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIV
ELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQSLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQMLKNLK
RDDSVGLGRRLLNHALGGSRKLAEVPAENIQHELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQGDQSNLGASSGVRNLA
IKGSDGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDQDIEQEFIAEIKVDMFNHQGA
LANLTAAINAAESNIQSLNTEEKDGRVYSAFIRLTTRDRIHLANIMRKIRIMPDVIKVNRNRN

Sequences:

>Translated_703_residues
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMRLDHETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFQDKKEAQAENFRKMIMAMVQDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHLKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLTEAGIPCRVSGREKHL
YSIYLKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKANGYQSLHTSLIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEKEQGETGTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIV
ELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQSLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQMLKNLK
RDDSVGLGRRLLNHALGGSRKLAEVPAENIQHELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQGDQSNLGASSGVRNLA
IKGSDGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDQDIEQEFIAEIKVDMFNHQGA
LANLTAAINAAESNIQSLNTEEKDGRVYSAFIRLTTRDRIHLANIMRKIRIMPDVIKVNRNRN
>Mature_703_residues
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMRLDHETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFQDKKEAQAENFRKMIMAMVQDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHLKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLTEAGIPCRVSGREKHL
YSIYLKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKANGYQSLHTSLIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEKEQGETGTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIV
ELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQSLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQMLKNLK
RDDSVGLGRRLLNHALGGSRKLAEVPAENIQHELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQGDQSNLGASSGVRNLA
IKGSDGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDQDIEQEFIAEIKVDMFNHQGA
LANLTAAINAAESNIQSLNTEEKDGRVYSAFIRLTTRDRIHLANIMRKIRIMPDVIKVNRNRN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=704, Percent_Identity=92.3295454545455, Blast_Score=1342, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=32.2108345534407, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25143118, Length=139, Percent_Identity=34.5323741007194, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24650996, Length=123, Percent_Identity=40.650406504065, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79267; Mature: 79267

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDHETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFQDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KMIMAMVQDIRVVLIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHLKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLTEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHH
YSIYLKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSLIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEKEQGETGTTAQIRAQRW
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHH
MQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHEEECCCCC
HACVGARVDRQPYPLSQSLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQMLKNLK
HHHHCCCCCCCCCCCCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RDDSVGLGRRLLNHALGGSRKLAEVPAENIQHELDRMKLATLDDLLAEIGLGNAMSVVVA
CCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEE
KNLQGDQSNLGASSGVRNLAIKGSDGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESC
CCCCCCCCCCCCCCCCCEEEEECCCCEEEEHHHHCCCCCCCCEEEEECCCCCEEEECHHH
RNIRGYQKEPEKFMAVEWDQDIEQEFIAEIKVDMFNHQGALANLTAAINAAESNIQSLNT
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
EEKDGRVYSAFIRLTTRDRIHLANIMRKIRIMPDVIKVNRNRN
CCCCCCEEEEEEEHHCCCHHHHHHHHHHHHHCHHHHHHCCCCC
>Mature Secondary Structure
MYLFESLNLLIQRYLPEEQIKRLKQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDHETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFQDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KMIMAMVQDIRVVLIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHLKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLTEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHH
YSIYLKMHLKEQRFHSIMDIYAFRVIVKEVDTCYRVLGQAHSLYKPRPGRVKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSLIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEKEQGETGTTAQIRAQRW
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHH
MQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHEEECCCCC
HACVGARVDRQPYPLSQSLTSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQMLKNLK
HHHHCCCCCCCCCCCCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RDDSVGLGRRLLNHALGGSRKLAEVPAENIQHELDRMKLATLDDLLAEIGLGNAMSVVVA
CCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEE
KNLQGDQSNLGASSGVRNLAIKGSDGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESC
CCCCCCCCCCCCCCCCCEEEEECCCCEEEEHHHHCCCCCCCCEEEEECCCCCEEEECHHH
RNIRGYQKEPEKFMAVEWDQDIEQEFIAEIKVDMFNHQGALANLTAAINAAESNIQSLNT
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
EEKDGRVYSAFIRLTTRDRIHLANIMRKIRIMPDVIKVNRNRN
CCCCCCEEEEEEEHHCCCHHHHHHHHHHHHHCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]