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The map label for this gene is priA [H]

Identifier: 157373021

GI number: 157373021

Start: 5307481

End: 5309676

Strand: Direct

Name: priA [H]

Synonym: Spro_4789

Alternate gene names: 157373021

Gene position: 5307481-5309676 (Clockwise)

Preceding gene: 157373019

Following gene: 157373022

Centisome position: 97.41

GC content: 61.29

Gene sequence:

>2196_bases
ATGCCTGTTGTTCACGTTGCTTTGCCGGTCCCCCTCGCCCGCACCTTCGACTATCTGCTGCCGCAAGGCCTGCAGCCGGC
GGTCGGAGCGCGCGTAGGTGTGCCCTGGGGTAAGCAACGGGCGATCGGTATCGTCACCGGCTGCAGCGAAACCAGCGAAT
TGCCGCTGGACAAGCTTAAACCCATCGACAGCGTGCTCGACAGCGCCTCGCTGTTCTCACCCAGCCTGTGGCGCATTCTG
CACTGGGCCAGCGACTATTATCATTACCCCATCGGTGAAGTGCTGTTCCACGCATTGCCTATCCTGCTGCGTCAGGGAAA
ACCGGCTGAAGCGGCACCGCTGTGGCAGTGGTTCGCTACCGAACAGGGCCGCGCTACGCTGCCGGAAAGCCTGAAACGCG
CGCCCAAGCAGCAGCAAGCGCTGGCCGCGTTGCTGCAACGTCCGGTCTATCGCCATCAGGTCAGTGAACTGGAATTAACC
GAGAGCGCCTTGCAGGCATTGCGCGCCAAGGGGCTGATCGATTTACGCTCTCAGACGTTGGCGGTGCAGGACTGGCGGCC
CGGTTTTGAAGTGCTGGGCGAACGGCTGCGGCTGAACACCGAGCAGGCGACCGCCGTGGGTGCCATTCGCAGCGAAGACG
AGCAGTTCGCCACCTGGCTGCTGGCCGGGGTCACGGGTTCCGGCAAGACCGAGGTCTATCTCAGCGTACTGGAAAACATC
CTGGCGAAGGGCAAACAGGCATTGGTACTGGTGCCGGAAATCGGCCTGACGCCGCAGACCATTGCCCGTTTTCGCGAACG
CTTCAACGCCCCGGTGGAGGTGCTGCACTCCGGCCTTAACGACAGTGAACGGCTGGCAGTCTGGCTGCGCGCCCGCAGCG
GCGAGGCGGCAATTGTTATCGGCACCCGCTCGGCGCTGTTTACGCCGTTTTCGCGCCTGGGCGTGATCATCATCGATGAA
GAACACGACAGCTCTTATAAACAGCAGGAAGGCTGGCGCTACCATGCCCGCGATCTGGCGGTATTCCGTGCCCGCGAGGA
AAACATCCCGATGGTGATGGGCACCGCCACGCCGGCACTGGAAACGCTGCACAATGTGCAACTGGGTAAATATCGCCAGC
TCAAACTCACCCAGCGTGCAGGTAATGCCAAACCGGCGACGCAGCATCTGATCGATCTGAAAGGCCTGCCGCTGAAAGTG
GGTCTTTCGCAGCCGTTGCTGAAAAGCATGCAGCACCATTTGAAGGCCGGTAATCAGGTGATGCTGTTTCTTAACCGGCG
CGGCTACGCACCGGCGCTGTTGTGCCACGAGTGCGGCTGGATCGCCGAGTGTCAGCGCTGCGATCATTACTACACCTTCC
ACCAGAATCATCGTCAACTGCGCTGCCACCACTGCGACAGCCAGCGTCCGGTCCCCCACCAGTGCCCACAATGCGGCTCT
ACTCATCTGGTGTCGGTCGGCGTCGGCACAGAACAGTTGGAACATGAACTGGCGCCGCTGTTCCCGGAAACGCCGATCAC
TCGTATCGATCGCGACACCACCAGCCGCAAAGGCTCGCTGGAACAGCATCTGGCGGATATTCACCGCGGCGGCTCGCGCA
TTTTGATCGGCACCCAGATGCTGGCCAAGGGCCACCATTTCCCGGATGTGACGTTGGTAGCCCTGCTGGATGTCGACGGC
GCTCTGTTCTCAGCCGACTTCCGTTCCGCCGAGCGCTTTGCCCAGCTGTATACCCAGGTCTCCGGCCGCGCCGGCCGTGC
CGGTAAGCCCGGCGAGGTCTTGTTGCAGACTCACCACCCGGAACACCCACTGCTGCAAATTCTGCTGCAACAGGGTTACG
ACGCCTTTGCCAAACAGACGTTGGCGGAGCGCAACAGCGTGTTCCTGCCGCCCTATACCAGCCATATCATCGTGCGTTCG
GAAGATCACGATAACCAGCAGGCCCCCCAGTTCCTGCAGCAACTGCGCAATCTGCTGGAAGCCAGCCCGCTGAAAGACGA
CTCGCTATGGGTGATGGGGCCGGTCCCGGCGCTGCAGTCGAAACGCGGCGGTCGTTTCCGCTGGCAATTGCTGCTGCAAC
ACCCTTCCCGGCGGGTACTGCAACAGTTAATGAAAAGCTCCCTGCCGCTGGTCAGTACCCTGCCACAGGCACGAAAGGTG
AAATGGACGCTGGATATCGATCCAATAGACAGCTGA

Upstream 100 bases:

>100_bases
GCAAATTATACAGAATTAAAAGTACCGCGCAATCGGAACCGTACATTTGCCGATGCGCAGTGTAAACTGATGGACTCTTT
TTTTCACTCTGGATGACCCC

Downstream 100 bases:

>100_bases
ACCCAGAAAAGTCCGATTCAGTTCATCCAATGCAAACGTTTACCCTTGAAATTGCGAGCGAACGCTAAAAAACCTTCCCA
GGTCACACTTTTTATGCAAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MPVVHVALPVPLARTFDYLLPQGLQPAVGARVGVPWGKQRAIGIVTGCSETSELPLDKLKPIDSVLDSASLFSPSLWRIL
HWASDYYHYPIGEVLFHALPILLRQGKPAEAAPLWQWFATEQGRATLPESLKRAPKQQQALAALLQRPVYRHQVSELELT
ESALQALRAKGLIDLRSQTLAVQDWRPGFEVLGERLRLNTEQATAVGAIRSEDEQFATWLLAGVTGSGKTEVYLSVLENI
LAKGKQALVLVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLAVWLRARSGEAAIVIGTRSALFTPFSRLGVIIIDE
EHDSSYKQQEGWRYHARDLAVFRAREENIPMVMGTATPALETLHNVQLGKYRQLKLTQRAGNAKPATQHLIDLKGLPLKV
GLSQPLLKSMQHHLKAGNQVMLFLNRRGYAPALLCHECGWIAECQRCDHYYTFHQNHRQLRCHHCDSQRPVPHQCPQCGS
THLVSVGVGTEQLEHELAPLFPETPITRIDRDTTSRKGSLEQHLADIHRGGSRILIGTQMLAKGHHFPDVTLVALLDVDG
ALFSADFRSAERFAQLYTQVSGRAGRAGKPGEVLLQTHHPEHPLLQILLQQGYDAFAKQTLAERNSVFLPPYTSHIIVRS
EDHDNQQAPQFLQQLRNLLEASPLKDDSLWVMGPVPALQSKRGGRFRWQLLLQHPSRRVLQQLMKSSLPLVSTLPQARKV
KWTLDIDPIDS

Sequences:

>Translated_731_residues
MPVVHVALPVPLARTFDYLLPQGLQPAVGARVGVPWGKQRAIGIVTGCSETSELPLDKLKPIDSVLDSASLFSPSLWRIL
HWASDYYHYPIGEVLFHALPILLRQGKPAEAAPLWQWFATEQGRATLPESLKRAPKQQQALAALLQRPVYRHQVSELELT
ESALQALRAKGLIDLRSQTLAVQDWRPGFEVLGERLRLNTEQATAVGAIRSEDEQFATWLLAGVTGSGKTEVYLSVLENI
LAKGKQALVLVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLAVWLRARSGEAAIVIGTRSALFTPFSRLGVIIIDE
EHDSSYKQQEGWRYHARDLAVFRAREENIPMVMGTATPALETLHNVQLGKYRQLKLTQRAGNAKPATQHLIDLKGLPLKV
GLSQPLLKSMQHHLKAGNQVMLFLNRRGYAPALLCHECGWIAECQRCDHYYTFHQNHRQLRCHHCDSQRPVPHQCPQCGS
THLVSVGVGTEQLEHELAPLFPETPITRIDRDTTSRKGSLEQHLADIHRGGSRILIGTQMLAKGHHFPDVTLVALLDVDG
ALFSADFRSAERFAQLYTQVSGRAGRAGKPGEVLLQTHHPEHPLLQILLQQGYDAFAKQTLAERNSVFLPPYTSHIIVRS
EDHDNQQAPQFLQQLRNLLEASPLKDDSLWVMGPVPALQSKRGGRFRWQLLLQHPSRRVLQQLMKSSLPLVSTLPQARKV
KWTLDIDPIDS
>Mature_730_residues
PVVHVALPVPLARTFDYLLPQGLQPAVGARVGVPWGKQRAIGIVTGCSETSELPLDKLKPIDSVLDSASLFSPSLWRILH
WASDYYHYPIGEVLFHALPILLRQGKPAEAAPLWQWFATEQGRATLPESLKRAPKQQQALAALLQRPVYRHQVSELELTE
SALQALRAKGLIDLRSQTLAVQDWRPGFEVLGERLRLNTEQATAVGAIRSEDEQFATWLLAGVTGSGKTEVYLSVLENIL
AKGKQALVLVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLAVWLRARSGEAAIVIGTRSALFTPFSRLGVIIIDEE
HDSSYKQQEGWRYHARDLAVFRAREENIPMVMGTATPALETLHNVQLGKYRQLKLTQRAGNAKPATQHLIDLKGLPLKVG
LSQPLLKSMQHHLKAGNQVMLFLNRRGYAPALLCHECGWIAECQRCDHYYTFHQNHRQLRCHHCDSQRPVPHQCPQCGST
HLVSVGVGTEQLEHELAPLFPETPITRIDRDTTSRKGSLEQHLADIHRGGSRILIGTQMLAKGHHFPDVTLVALLDVDGA
LFSADFRSAERFAQLYTQVSGRAGRAGKPGEVLLQTHHPEHPLLQILLQQGYDAFAKQTLAERNSVFLPPYTSHIIVRSE
DHDNQQAPQFLQQLRNLLEASPLKDDSLWVMGPVPALQSKRGGRFRWQLLLQHPSRRVLQQLMKSSLPLVSTLPQARKVK
WTLDIDPIDS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=731, Percent_Identity=73.734610123119, Blast_Score=1124, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=222, Percent_Identity=29.2792792792793, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81820; Mature: 81689

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVHVALPVPLARTFDYLLPQGLQPAVGARVGVPWGKQRAIGIVTGCSETSELPLDKLK
CCEEEEEECCCHHHHHHHHCCCCCCCHHCCEECCCCCCCCEEEEEECCCCCCCCCHHHHC
PIDSVLDSASLFSPSLWRILHWASDYYHYPIGEVLFHALPILLRQGKPAEAAPLWQWFAT
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHC
EQGRATLPESLKRAPKQQQALAALLQRPVYRHQVSELELTESALQALRAKGLIDLRSQTL
CCCCCCCCHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEE
AVQDWRPGFEVLGERLRLNTEQATAVGAIRSEDEQFATWLLAGVTGSGKTEVYLSVLENI
EEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHEECCCCCCHHHHHHHHHHH
LAKGKQALVLVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLAVWLRARSGEAAIVI
HHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEECCCCEEEEE
GTRSALFTPFSRLGVIIIDEEHDSSYKQQEGWRYHARDLAVFRAREENIPMVMGTATPAL
ECCHHHHCCHHHCCEEEEECCCCCCHHHHCCCEEHHHHHHHHEECCCCCCEEEECCCHHH
ETLHNVQLGKYRQLKLTQRAGNAKPATQHLIDLKGLPLKVGLSQPLLKSMQHHLKAGNQV
HHHHHCCCCCCCEEEEHHHCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCEE
MLFLNRRGYAPALLCHECGWIAECQRCDHYYTFHQNHRQLRCHHCDSQRPVPHQCPQCGS
EEEEECCCCCCHHHHHHCCCHHHHHHCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCC
THLVSVGVGTEQLEHELAPLFPETPITRIDRDTTSRKGSLEQHLADIHRGGSRILIGTQM
CEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHH
LAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKPGEVLLQTHHP
HHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCC
EHPLLQILLQQGYDAFAKQTLAERNSVFLPPYTSHIIVRSEDHDNQQAPQFLQQLRNLLE
CCHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
ASPLKDDSLWVMGPVPALQSKRGGRFRWQLLLQHPSRRVLQQLMKSSLPLVSTLPQARKV
CCCCCCCCEEEEECCCHHHCCCCCCEEEHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCEE
KWTLDIDPIDS
EEEEECCCCCC
>Mature Secondary Structure 
PVVHVALPVPLARTFDYLLPQGLQPAVGARVGVPWGKQRAIGIVTGCSETSELPLDKLK
CEEEEEECCCHHHHHHHHCCCCCCCHHCCEECCCCCCCCEEEEEECCCCCCCCCHHHHC
PIDSVLDSASLFSPSLWRILHWASDYYHYPIGEVLFHALPILLRQGKPAEAAPLWQWFAT
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHC
EQGRATLPESLKRAPKQQQALAALLQRPVYRHQVSELELTESALQALRAKGLIDLRSQTL
CCCCCCCCHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEE
AVQDWRPGFEVLGERLRLNTEQATAVGAIRSEDEQFATWLLAGVTGSGKTEVYLSVLENI
EEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHEECCCCCCHHHHHHHHHHH
LAKGKQALVLVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLAVWLRARSGEAAIVI
HHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEECCCCEEEEE
GTRSALFTPFSRLGVIIIDEEHDSSYKQQEGWRYHARDLAVFRAREENIPMVMGTATPAL
ECCHHHHCCHHHCCEEEEECCCCCCHHHHCCCEEHHHHHHHHEECCCCCCEEEECCCHHH
ETLHNVQLGKYRQLKLTQRAGNAKPATQHLIDLKGLPLKVGLSQPLLKSMQHHLKAGNQV
HHHHHCCCCCCCEEEEHHHCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCEE
MLFLNRRGYAPALLCHECGWIAECQRCDHYYTFHQNHRQLRCHHCDSQRPVPHQCPQCGS
EEEEECCCCCCHHHHHHCCCHHHHHHCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCC
THLVSVGVGTEQLEHELAPLFPETPITRIDRDTTSRKGSLEQHLADIHRGGSRILIGTQM
CEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHH
LAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKPGEVLLQTHHP
HHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCC
EHPLLQILLQQGYDAFAKQTLAERNSVFLPPYTSHIIVRSEDHDNQQAPQFLQQLRNLLE
CCHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
ASPLKDDSLWVMGPVPALQSKRGGRFRWQLLLQHPSRRVLQQLMKSSLPLVSTLPQARKV
CCCCCCCCEEEEECCCHHHCCCCCCEEEHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCEE
KWTLDIDPIDS
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]